Share / Export Citation / Email / Print / Text size:

Architecture, Civil Engineering, Environment

Silesian University of Technology

Subject: Architecture, Civil Engineering, Engineering, Environmental


ISSN: 1899-0142





Volume / Issue / page

Related articles

VOLUME 9 , ISSUE 1 (March 2016) > List of articles


Justyna Michalska * / Danuta Wojcieszyńska / Izabela Greń

Keywords : Activated sludge, Functional diversity indices, Biolog® Ecoplate, Excess sludge, Wastewater Treatment Plant

Citation Information : Architecture, Civil Engineering, Environment. Volume 9, Issue 1, Pages 119-126, DOI:

License : (BY-NC-ND 4.0)

Published Online: 09-August-2018



Activated sludge method dominates among the currently used biological wastewater treatment systems. The dynamic development of this technology implies need for research, most of which are carried out in laboratory conditions. Biolog system seems to be an effective tool that allows a quick assessment of microbial activity of activated sludge. The aim of this study was to verify Biolog® EcoPlates’ usage for comparing the functional and metabolic diversity of activated and excess sludge microbial communities, collected from WWTP “Klimzowiec” in Chorzów with the excess sludge subjected to the purification process, and then long-term adaptation in laboratory conditions. Changes in the functional diversity of activated sludge
during the adaptation in the lab-scale were verified. It was found that the acclimatization process plays a crucial role in sludge adaptation to the laboratory environment. In addition, it has been shown that subjecting the excess sludge to purification may lead to changes in functional diversity of this community.


Content not available PDF Share



Amman R., Lemmer H. Wagner M.; Monitoring the community structure of wastewater treatment plants: a comparison of old and new techniques. FEMS
Microbiology Ecology, 25, 1998; p.205-215

Keakewipat K., Grady Jr. C.P.L.; Microbial population dynamics in labolatory-scale activated sludge reactors. Water Science and Technology, 46, 2002; p.19-27

Al-Mutairi N.Z.; Variable distributional characteristics of substrate utilization patterns in activated sludge plants in Kuwait Bioresource Technology, 100,
2009; p.1524-1532

Yang C., Zhang W., Liu R., Li Q., Li B., Wang S., Song C., Qiao C., Mulchandani A.; Phylogenetic diversity phylogenetic diversity and metabolic potential of activated sludge microbial communities in full-scale wastewater treatment plants. Environmental Science and Technology, 45, 2011; p.7408-7415

Barbusinski K.; Adaptation of activated sludge to laboratory research conditions. Environmental Protection Engineering, 17, 1991; p.57-65

van der Gast C.J., Whiteley A.S., Thompson I.P.; Temporal dynamics and degradation activity of an bacterial inoculum for treating waste metal-working
fluid. Environmental Microbiology, 6(3), 2004; p.254-263

Forney L. J., Liu W. T., Guckert J. B., Kumagai Y., Namkung E., Nishihara T., Larson R. J.; Structure of microbial communities in activated sludge: potential
implications for assessing the biodegradability of chemicals. Ecotoxicology and Environmental Safety, 49, 2001; p.40-53

Kim T.S, Jeong J.Y, Wells G.F., Park H.D.; General and rare bacterial taxa demonstrating different temporal dynamic patterns in an activated sludge bioreactor. Applied Microbiology and Biotechnology, 97, 2013; p.1755-1765

Jenkins D., Richard M. G., Daigger G. T.; Manual on the causes and control of activated sludge bulking and foaming. Lewis Publishers, Michigan, USA, 1993
Kaiser S., Guckert J., Gledhill D.; Comparison of activated sludge microbial communities using BiologTM microplates. Water Science and Technology, 37(4-5), 1998; p.57-63

Garland J. L., Mills A. L.; Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization. Applied and Environmental Microbiology, 57(8), 1991; p.2351-2359

Insam H., Rangger A.; Microbial communities: functional versus structural approaches. Springer-Verlag, Berlin, Germany, 1997

Preston-Mafham J., Boddy L., Randerson P. F.;Analysis of microbial community functional diversity using sole-carbon-source utilisation profiles – a critique. FEMS Microbiology Ecology, 42, 2002; p.1-14

Papadimitriou CH., Palaska G., Lazaridou M., Samaras P., Sakellaropoulos, G.P.; The effects of toxic substances on the activated sludge microfauna.
Desalination, 211, 2007; p.177-191

American Public Health Association, American Water Works Association, and Water Environment Federation; Standard Methods for the Examination
of Water and Wastewater, 20th edition. American Public Health Association, Washington, D.C., 1998

Garland J.L.; Analysis and interpretation of community-level physiological profiles in microbial ecology. FEMS Microbiology Ecology, 24, 1997; p.289-300

Allen M. J., Edberg S. C., Reasoner D., J.; Heterotrophic plate count bacteria-what is their significance in drinking water? International Journal of Food Microbiology, 92(3), 2004; p.265-274

Wagner M., Loy A.; Bacterial community composition and function in sewage treatment systems. Current Opinion in Biotechnology, 13, 2002; p.218-227

van der Broeck R.M.R., van Impe J.F.M., Smets I.Y.M.; Assessment of activated sludge stability in lab-scale experiments. Journal of Biotechnology, 141, 2009; p.147-154

Zawani Z., Chuah-Abdullah L., Ahmadun F.R., Abdan K.; Acclimatization process of microorganisms from activated sludge in Kenaf-Retting Wastewater.
Developments in Sustainable Chemical and Bioprocess Technology, 2013; p.59-64

Urbain V., Pys E., Block J.C., Manem J.; Composition and activity of activated sludge under starvation conditions. Environmental Technology, 14, 1993; p.731-740

Chetty M., Mudaly D.D., Bux F.; Manipulation of activated sludge for the direct detection of polyphosphate accumulating bacteria. WISA 2000 Biennial
Conference, Sun City, South Africa, 2000

Mendrycka M., Stawarz M.; Possibilities of applications of biocomponent for biological processes of tanning sewage by activated sludge-aided method.
Inżynieria Ekologicza, 28, 2012; p.43-56

Babko R., Kuzmina T., Jaromin-Gleń K., Bieganowski A.; Bioindication assessment of activated sludge adaptation in a lab-scale experiment. Ecological Chemistry and Engineering, 21(4), 2014; p.605-616

Muszyński A., Załęska-Radziwiłł M.; Polyphosphate accumulating organisms in treatment plants with different wastewater composition. Architecture Civil Engineering Environment, 4, 2015; p.605-616

Webber K.P., Legge R.L.; One-dimensional metric for Fu Methods 79, 2009; p.55-61


Wilson W.A., Roach P.J., Montenero M., Baroja-Fernández E., Muñoz J., Eydallin G., Viale A.M., Pozueta-Romero J.; Regulation of glycogen metabolism in yeast and bacteria. FEMS Microbiology Reviews, 34(6), 2010; p.952-985

Palm J., Jenkins D.; Relationship between organic loading, dissolved oxygen concentration and sludge settleability in the completely mixed activated sludge process. Journal of Water Pollution Control Federation, 52(10),1980; p.2484-2506