Sulfhydryl treatment of serum or plasma for the reduction of IgM antibodies

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 34 , ISSUE 4 (December 2018) > List of articles

Sulfhydryl treatment of serum or plasma for the reduction of IgM antibodies

Lorraine N. Blagg *

Keywords : dithiothreitol, 2-mercaptoethanol, IgM antibodies

Citation Information : Immunohematology. Volume 34, Issue 4, Pages 135-139, DOI: https://doi.org/10.21307/immunohematology-2018-021

License : (Transfer of Copyright)

Published Online: 16-October-2019

ARTICLE

ABSTRACT

Dithiothreitol (DTT) and 2-mercaptoethanol (2-ME) are sulfhydryl compounds that can be used to treat serum or plasma to denature IgM antibody reactivity. By using sulfhydryl agents, IgG and IgM antibodies can be separated, the relative amount of IgM and IgG antibodies can be determined, and the risk of hemolytic disease of the fetus and newborn can be assessed.

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