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Citation Information : Immunohematology. Volume 4, Issue 1, Pages 5-9, DOI: https://doi.org/10.21307/immunohematology-2019-1085
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Published Online: 29-December-2020
Both anaphylactic and anaphylactoid reactions to transfused blood products and plasma derivatives have occurred in IgA-deficient patients with antibodies to IgA. This study reports the concentration of IgA in various blood products to assist in the development of treatment protocols for IgA-deficient patients. Five types of blood products and seven types of plasma derivatives were tested for the presence of IgA using a semiquantitative hemagglutination inhibition method. The concentration of lgA was measured by radial immunodiffusion and enzyme-linked immunosorbent assay techniques. Blood products which consistently contained IgA less than 0.05 mg/dL (the present definition for IgA deficiency used by the American Red Cross Rare Donor Registry) were from IgA-deficient donors, deglycerolized red blood cells (RBCs) prepared with an extra wash cycle, and RBCs washed using a total volume of approximately 1300 mL of 0.9 percent sodium chloride.