ABO and Rh(D) blood typing on the PK 7200 with ready-to-use kits

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 19 , ISSUE 2 (June 2003) > List of articles

ABO and Rh(D) blood typing on the PK 7200 with ready-to-use kits

Pierre Moncharmont / Annick Plantier / Véronique Chirat / Dominique Rigal

Keywords : ABO, Rh(D), PK 7200 automated blood grouping system

Citation Information : Immunohematology. Volume 19, Issue 2, Pages 54-56, DOI: https://doi.org/10.21307/immunohematology-2019-475

License : (Transfer of Copyright)

Published Online: 14-October-2020

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ABSTRACT

The performance of ready-to-use kits was evaluated on the PK 7200 blood grouping system. The Olymp Group (kit 1) and Olymp Group II (kit 2) containing anti-A, -B, -AB, and -D reagents were tested for first and second determinations of A, B, and D antigens. More than 500 RBC samples, including several variant ABO and D phenotypes, were evaluated for specificity, repeatability, reproducibility, and sensitivity. Specificity was tested with wellcharacterized reagent RBCs. Repeatability was established by at least 12 assays per run with three reagent RBCs,and reproducibility was established on one run per day for 5 days. No discrepancy was observed in ABO and D determinations with either kit. In repeatability, three discrepancies were found with group A and B RBCs with kit 1. In reproducibility, no discrepancies were observed. The kit 1 anti-A reagent detected A3 but not Ax RBCs and anti-AB detected both. A B3 RBC was detected by both kits. Among eight weak D phenotypes, six were positive with kit 1. With kit 2, only one of five weak D phenotypes was detected.

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