DNA analysis for donor screening of Dombrock blood group antigens

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 19 , ISSUE 3 (September 2003) > List of articles

DNA analysis for donor screening of Dombrock blood group antigens

Jill R. Storry / Connie M. Westhoff / Dalisay Charles-Pierre / Maria Rios / Kim Hue-Roye / Sunitha Vege / Sandra Nance / Marion E. Reid

Keywords : Dombrock blood group system, Dombrock polymorphisms Doa /Dob , PCR-RFLP, typing donors

Citation Information : Immunohematology. Volume 19, Issue 3, Pages 73-76, DOI: https://doi.org/10.21307/immunohematology-2019-480

License : (Transfer of Copyright)

Published Online: 14-October-2020

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ABSTRACT

Due to the scarcity of reliable antibodies, RBC typing for Doa and Dob is notoriously difficult. Inaccurate typing can place patients at risk for hemolytic transfusion reactions. The molecular basis of the DOA/DOB polymorphism is associated with three nucleotide changes:378 C>T,624 T>C, and 793 A>G of DO. While the 378 C>T and 624 T>C are silent mutations, the 793A>G polymorphism in codon 265 encodes asparagine for Doa and aspartic acid for Dob . We describe here the use of a PCR-RFLP assay as an alternative to traditional hemagglutination for typing donor blood for Dombrock. Primers were designed to amplify the region of DO containing the 793A>G polymorphism. DNA samples from blood donors were amplified and subjected to RFLP analysis. A total of 613 samples were tested for the Dombrock polymorphism (793 A>G) by PCRRFLP. PCR-RFLP can be used to screen for Do(a–) or Do(b–) donors. This approach overcomes the scarcity of the reagents required for testing by hemagglutination.

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