A comparison of a new affinity column system with a conventional tube LISS-antiglobulin test for antibody detection

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 15 , ISSUE 2 (June 1999) > List of articles

A comparison of a new affinity column system with a conventional tube LISS-antiglobulin test for antibody detection

R. Sue Shirey / Joan S. Boyd / Christine Barrasso / Karen E. King / Paul M. Ness

Keywords : affinity column, microcolumn, antibody detection

Citation Information : Immunohematology. Volume 15, Issue 2, Pages 75-77, DOI: https://doi.org/10.21307/immunohematology-2019-618

License : (Transfer of Copyright)

Published Online: 26-October-2020

ARTICLE

ABSTRACT

A recently introduced system for antibody detection (ReACT™) consists of affinity columns (AFC) that contain protein A and protein Gcoated agarose. We compared the ReACT™ system to a conventional tube low-ionic-strength saline antiglobulin test (LISS-AGT). We selected 100 LISS-AGT positive samples with clinically important and benign antibodies of varying strengths and 130 LISS-AGT negative samples to evaluate by the AFC method. AFC tests were positive with all 84 clinically important antibodies, including 36 antibodies that reacted ≤ 1+ at LISS-AGT (0% falsely negative). Eleven of 16 (69%) clinically benign antibodies reacted by AFC. Five samples (2 anti-Sda, 2 anti-I, and 1 inconclusive) were negative by AFC. AFC tests were negative with all 130 samples that were negative by LISS-AGT (0% falsely positive). The AFC method showed results comparable with results obtained with a conventional tube LISS-AGT for detection of clinically important antibodies. Some unwanted, clinically benign antibodies may not be detected by the AFC method.

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