Glycine-EDTA treatment to prepare Ko red blood cells: preservation of high-frequency antigens

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 10 , ISSUE 2 (June 1994) > List of articles

Glycine-EDTA treatment to prepare Ko red blood cells: preservation of high-frequency antigens

Jana Julleis / Cindy Sapp / Ram Kakaiya

Citation Information : Immunohematology. Volume 10, Issue 2, Pages 64-65, DOI: https://doi.org/10.21307/immunohematology-2019-921

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Published Online: 22-November-2020

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ABSTRACT

Reactivity of high-incidence antigens after glycine-EDTA treatment of red blood cells (RBCs) to prepare artificial Ko RBCs was investigated. The treatment had little or no effect on Yta, JMH, Yka, Kna, and McCa. Hy antigen-positive-treated RBCs reacted less well with anti-Hy when compared to nontreated cells. Glycine-EDTA treatment to prepare Ko RBCs offers an advantage over AET treatment because it preserves some high-frequency antigens that AET treatment does not.

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