Antibody screening and direct antiglobulin testing using BioVueTM column agglutination technology versus standard tube tests

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 9 , ISSUE 3 (September 1993) > List of articles

Antibody screening and direct antiglobulin testing using BioVueTM column agglutination technology versus standard tube tests

Susan F. South

Citation Information : Immunohematology. Volume 9, Issue 3, Pages 78-80, DOI: https://doi.org/10.21307/immunohematology-2019-963

License : (Transfer of Copyright)

Published Online: 06-December-2020

ARTICLE

ABSTRACT

The BioVue™ column agglutination technology (CAT) was evaluated simultaneously with standard tube test (STT) methodology for use in indirect antiglobulin testing (IAT) and direct antiglobulin testing (DAT). One thousand thirty-five blood specimens were used for the IAT comparison, and 44 blood specimens were used for the DAT comparison. Both polyspecific antiglobulin and anti-IgG antiglobulin reagents were used in the tube testing and the CAT testing. For IAT, sensitivity was 100 percent for CAT and 99.6 percent for STT; sensitivity was 97.9 percent for CAT and 100 percent for STT. In addition, a 67 percent labor savings was realized with CAT versus STT. Specificity and sensitivity of both methodologies were 100 percent for the DAT. BioVue™ proved to be a reliable and efficient alternative to standard test tube methods for doing IATs and DATs.

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