Genotyping for Dombrock blood group alleles in Northern Pakistani blood donors

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 37 , ISSUE 3 (Sep 2021) > List of articles

Genotyping for Dombrock blood group alleles in Northern Pakistani blood donors

S.A. Jadoon * / N. Salamat / S.A. Khan / M.S. Yazdani / N. Khatak / M.A. Naeem

Keywords : Dombrock, red blood cell antigens, sequence-specific primer polymerase chain reaction (PCR-SSP), genotyping

Citation Information : Immunohematology. Volume 37, Issue 3, Pages 113-117, DOI: https://doi.org/10.21307/immunohematology-2021-016

License : (Transfer of Copyright)

Published Online: 30-September-2021

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ABSTRACT

Genotyping can be used to identify rare blood group antigens and to solve suspected blood group discrepancies, particularly when serologic methods are limited. Unfortunately, only a few such studies have been performed in Pakistan. The present study was conducted to determine the frequency of Dombrock blood group alleles by genotyping samples from blood donors from the north of Pakistan. Blood samples were taken with consent from 300 blood donors; DNA was extracted and tested for DO*01 and DO*02 alleles by sequence-specific primer polymerase chain reaction (PCR-SSP), followed by gel electrophoresis. Allele frequencies were calculated. The observed and expected genotype frequencies were compared using the χ2 test. The allele frequencies for DO*01 and DO*02 were 0.40 and 0.60, respectively. Genotype frequencies were in Hardy-Weinberg equilibrium. This study in Pakistani blood donors provides Dombrock blood group allele frequencies by PCR-SSP. This approach is efficient and economical and can be applied in developing countries. The findings can contribute to the development of in-house red blood cell panels, identification of rare blood types, and establishment of a national rare blood donor program.

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