Esteya vermicola Controls the Pinewood Nematode, Bursaphelenchus xylophilus, in Pine Seedlings

Sign In

  1. Home
  2. Publications
  3. Journal of Nematology
  4. Publication Articles
  5. Article

Publications

Share / Export Citation / Email / Print / Text size:

Journal of Nematology

Society of Nematologists

Subject: Life Sciences

GET ALERTS DONATE

ISSN: 0022-300X
eISSN: 2640-396X

DESCRIPTION

Article Metrics
59
Reader(s)
117
Visit(s)
0
Comment(s)
0
Share(s)

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue / page

Archive
Volume 54 (2022)
Volume 53 (2021)
Volume 52 (2020)
Volume 51 (2019)
Volume 50 (2018)
Volume 49 (2017)
Volume 48 (2016)
Related articles

VOLUME 49 , ISSUE 1 (March 2017) > List of articles

Esteya vermicola Controls the Pinewood Nematode, Bursaphelenchus xylophilus, in Pine Seedlings

ZHEN WANG * / YONGAN ZHANG / CHUNYAN WANG / YUNBO WANG / CHUNGKEUN SUNG

Keywords : biological control, Esteya vermicola, pinewood nematode, survival rate, wound

Citation Information : Journal of Nematology. Volume 49, Issue 1, Pages 86-91, DOI: https://doi.org/10.21307/jofnem-2017-048

License : (CC BY 4.0)

Received Date : 22-November-2016 / Published Online: 21-July-2017

  • ARTICLE
  • FIGURES & TABLES
  • REFERENCES
  • EXTRA FILES
  • COMMENTS

ARTICLE

ABSTRACT

Esteya vermicola (Ophiostomataceae) is an endoparasitic fungus that has great potential as a biological control agent
against the pinewood nematode Bursaphelenchus xylophilus which causes pine wilt disease.We tested E. vermicola for control of pine wilt disease by spraying E. vermicola conidia on artificial wounds on pine seedlings, and the optimum E. vermicola treatment density and application time were also investigated in the greenhouse. The wounds were similar to those made by sawyer beetles (Coleoptera:Cerambycidae). Esteya vermicola treatments significantly increased the survival rate of pine seedlings that were infected by pinewood nematodes.Wounded plants sprayed with 107 CFU/ml E. vermicola had a 73.0% greater survival rate than nonwounded pine seedlings treated similarly. The treatment of pine seedlings with 107 CFU/ml E. vermicola 14 d before nematode infection increased their survival rate by 90.0%. The number of pinewood nematodes isolated from dead pine seedlings sprayed with E. vermicola was 76% less than the number of pinewood nematodes in the controls. Moreover, infected nematodes and the hyphae of E. vermicola were detected in the dead or wilting pine seedlings. Therefore, spraying E. vermicola on the wounds of pine seedlings made by sawyer beetles provides good control of the pine wilt disease that is caused by pinewood nematodes.

PDF Share

Content not available PDF Share

FIGURES & TABLES

REFERENCES

  1. Abelleira, A., Picoaga, A., Mansilla, J. P., and Aguin, O. 2011. Detection of Bursaphelenchus xylophilus, causal agent of pine wilt disease on Pinus pinaster in Northwestern Spain. Plant Disease 95:776.
  2. Bouchier, R. J. 1961. Laboratory studies on crofungi isolated from the stems of living lodgepole pine Pinus contorta Dougl. Canadian Journal of Botany 39:1373–1385.
  3. Donald, P. A., Stamps, W. T., and Linit, M. J. 2003. Pine wilt disease in APSnet Plant Disease Instructor. St. Paul, MN: The American Phytopathological Society. DOI:10.1094/PHI-I-2003-0130-01.
  4. Dwinell, L. D. 1997. The pinewood nematode: Regulation and mitigation. Annual Review of Phytopathology 35:153–166.
  5. Fukushige, H. 1991. Propagation of Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) on fungi growing in pine-shoot segments. Applied Entomology and Zoology 26:371–376.
  6. Kawazu, K., Zhang, H., Yamashita, H., and Kazaki, H. 1996. Relationship between the pathogenicity of the pine wood nematode, Bursaphelenchus xylophilus, and phenylacetic acid production. Bioscience,Biotechnology, and Biochemistry 60:1413–1415.
  7. Kazuya, T., Toshio, S., and Kazuyoshi, K. 2003. Development and preventative effect against pine wilt disease of a novel liquid formulation of emamectin benzoate. Pest Management Science 59:365–370.
  8. Kikuchi, T., Shibuya, H., and Jones, J. T. 2005. Molecular and biochemical characterization of an endo-b-1,3-glucanase from the pinewood nematode Bursaphelenchus xylophilus acquired by horizontal gene transfer from bacteria. Biochemical Journal 389:117–125.
  9. Kiyohara, T., and Tokushige, Y. 1971. Inoculation experiments of a nematode, Bursaphelenchus sp., onto pine trees (in Japanese with English summary). Journal of the Japanese Forest Society 53:210–218.
  10. Kobayashi, T., Sasaki, K., and Mamiya, Y. 1974. Fungi associated with Bursaphelenchus lignicolus, the pine wood nematode (in Japanese with English abstract). Journal of the Japanese Forest Society 56:136–145.
  11. Kong, J. O., Park, I. K., Choi, K. S., Shin, S. C., and Ahn, Y. J. 2007. Nematicidal and propagation activities of thyme red and white oil compounds toward Bursaphelenchus xylophilus (Nematoda: Parasitaphelenchidae).
    Journal of Nematology 39:237–242.
  12. Kwon, T. S., Shin, J. H., Lim, J. H., Kim, Y. K., and Lee, E. J. 2011. Management of pine wilt disease in Korea through preventative silvicultural control. Forest Ecology and Management 261:562–569.
  13. Kwon, T. S., Song, M. Y., Shin, S. C., and Park, Y. S. 2005. Effects of aerial insecticide sprays on ant communities to control pine wilt disease in Korean pine forests. Applied Entomology and Zoology 40:563–574.
  14. Lee, S. M., Chung, Y. J., Moon, Y. S., Lee, S. G., Lee, D. W.,Choo, H. Y., and Lee, C. K. 2003. Insecticidal activity and fumigation conditions of several insecticides against Japanese pine sawyer (Monochamus alternatus) larvae. Journal of Korean Forestry Society 92:191–198.
  15. Lin, F., Ye, J. L., Wang, H. G., Zhang, A. J., and Zhao, B. G. 2013. Host deception: Predaceous fungus, Esteya vermicola, entices pinewood nematode by mimicking the scent of pine tree for nutrition.PLoS One 8:e71676.
  16. Liou, J. Y., Shih, J. Y., and Tzean, S. S. 1999. Esteya, a new nematophagous genus from Taiwan, attacking the pinewood nematode (Bursaphelenchus xylophilus). Mycological Research 103:242–248.
  17. Luıs, F. B., Edmundo, S., Pedro, N., Maria, L. I., Joana, H., Manuel, M., Pedro, B., Mike, J. D., and Stanislas, B. 2013. Efficacy of sulfuryl fluoride against the pinewood nematode, Bursaphelenchus xylophilus (Nematoda: Aphelenchidae), in Pinus pinaster boards. Pest Management Science 70:6–13.
  18. Maehara, N. 2008. Reduction of Bursaphelenchus xylophilus (Nematoa:Parasita phelenchidae) population by inoculating Trichoderma spp. into pine wilt-killed trees. Biological Control 44:61–66.
  19. Maehara, N., and Futai, K. 2000. Population changes of the pinewood nematode, Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae), on fungi growing in pine-branch segments. Applied Entomology
    and Zoology 35:413–417.
  20. Mamiya, Y., and Enda, N. 1972. Transmission of Bursaphelenchus lignicolus (Nematoda: Aphelenchoididae) by Monochamus alternatus (Coleoptera: Cerambycidae). Nematologica 18:159–162.
  21. Meyer, S. L. F., Massoud, S. I., Chitwood, D. J., and Roberts, D. P.2000. Evaluation of Trichoderma virens and Burkholderia cepacia for antagonistic activity against root-knot nematode, Meloidogyne incognita. Nematology 2:871–879.
  22. Nose, M., and Shiraishi, S. 2008. Breeding for resistance to pine wilt disease Pp. 334–350. in B. G Zhao, K. Futai, J. R. Sutherland, and Y. Takeuchi, eds. Pine wilt disease. Tokyo, Japan: Springer.
  23. Ogura, N., and Nakashima, T. 2002. In vitro occurrence of dispersal fourth stage juveniles in Bursaphelenchus xylophilus co-incubated with Monochamus alternates. Japanese Journal of Nematology 2:53–59.
  24. Reid, R. W., Whitney, H. S., and Watson, J. A. 1967. Reaction of lodgepole pine to attack by Dendroctonus ponderosae Hopkins and blue stain fungi. Canadian Journal of Botany 45:1115–1126.
  25. Saiki, H., Saito, T., Yoneda, K., Kaijo, M., Uchida, K., and Yamanaka, K. 1984. Biological control of the pinewood nematode by spraying a nematode-trapping fungus. Journal of the Japanese Forest Society 66:30–32.
  26. Wang, L. P. 2004. Study on the biological characteristic of Monochamus Alternatus hope (in Chinese). Journal of Fujian Forestry Science and Technology 31(3):23–26.
  27. Wang, C. Y., Fang, Z. M., Sun, B. S., Gu, L. J., Zhang, K. Q., and Sung, C. K. 2008. High Infectivity of an endoparasitic fungus strain, Esteya vermicola, against nematodes. The Journal of Microbiology 46:380–389.
  28. Wang, C. Y., Fang, Z. M., Wang, Z., Zhang, D. L., Gu, L. J.,Lee, M. R., Liu, L., and Sung, C. K. 2011a. Biological control of the pinewood nematode Bursaphelenchus xylophilus by application of the endoparasitic fungus Esteya vermicola. BioControl 56:91–100.
  29. Wang, Z., Wang, C. Y., Fang, Z. M., Zhang, D. L., Liu, L., Lee, M. R., Li, Z., Li, J. J., and Sung, C. K. 2010. Advances in research of pathogenic mechanism of pine wilt disease. African Journal of Microbiology Research 4:437–442.
  30. Wang, Z., Wang, C. Y., Gu, L. J., Wang, Y. B., Zhang, Y. A., and Sung, C. K. 2011b. Growth of Esteya vermicola in media amended with nitrogen sources yields conidia with increased predacity and resistance to environmental stress. Canadian Journal of Microbiology 57:838–843.

EXTRA FILES

COMMENTS