Sampling Error in Relation to Cyst Nematode Population Density Estimation in Small Field Plots

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Journal of Nematology

Society of Nematologists

Subject: Life Sciences

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ISSN: 0022-300X
eISSN: 2640-396X

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VOLUME 49 , ISSUE 2 (June 2017) > List of articles

Sampling Error in Relation to Cyst Nematode Population Density Estimation in Small Field Plots

VESNA ZUPUNSKI * / RADIVOJE JEVTIC / VESNA SPASIC JOKIC / LJUBICA ZUPUNSKI / MIRJANA LALOSEVIC / MIHAJLO CIRIC / ZIVKO CURCIC

Keywords : cyst nematodes, detection, Heterodera schachtii, population density, small field plots.

Citation Information : Journal of Nematology. Volume 49, Issue 2, Pages 150-155, DOI: https://doi.org/10.21307/jofnem-2017-059

License : (CC BY 4.0)

Received Date : 11-December-2016 / Published Online: 24-July-2017

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ABSTRACT

Cyst nematodes are serious plant-parasitic pests which could cause severe yield losses and extensive damage. Since there is still very little information about error of population density estimation in small field plots, this study contributes to the broad issue of population density assessment. It was shown that there was no significant difference between cyst counts of five or seven bulk samples taken per each 1-m2 plot, if average cyst count per examined plot exceeds 75 cysts per 100 g of soil. Goodness of fit of data to probability distribution tested with x2 test confirmed a negative binomial distribution of cyst counts for 21 out of 23 plots. The recommended measure of sampling precision of 17% expressed through coefficient of variation (cv) was achieved if the plots of 1m2 contaminated with more than 90 cysts per 100 g of soil were sampled with 10-core bulk samples taken in five repetitions. If plots were contaminated with less than 75 cysts per 100 g of soil, 10-core bulk samples taken in seven repetitions gave cv higher than 23%. This study indicates that more attention should be paid on estimation of sampling error in experimental field plots to ensure more reliable estimation of population density of cyst nematodes.

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REFERENCES

  1. Barker, K. R., and Campbell, C. L. 1981. Sampling nematode populations.Pp. 451–473 in B. M. Zuckerman and R. A. Rohde, eds. Plant parasitic nematodes, vol. 3. London: Academic Press.
    [CROSSREF]
  2. Been, T. H., and Schomaker, C. H. 2000. Development and evaluation of sampling methods for fields with infestation foci of potato cyst nematode (Globodera rostochiensis and G. pallida). Phytopathology 90:647–656.
    [CROSSREF]
  3. Campbell, C. L., and Benson, D. M. 1994. Spatial aspects of the development of root disease epidemics. Pp. 195–243 in C. L. Campbell and D. M. Benson, eds. Epidemiology and management of root diseases. Berlin Heidelberg: Springer-Verlag.
    [CROSSREF]
  4. Ferris, H. 1984. Nematode damage functions: The problems of experimental and sampling error. Journal of Nematology 16(1):1–9.
  5. Francl, L. J. 1986. Spatial Analysis of Heterodera glycines populations in field plots. Journal of Nematology 18(2):183–189.
  6. Heijbroek, L., Munning, R. G., and van Swaaij, A. C. P. M. 2002. The effect of different levels of beet cyst nematodes (Heterodera schachtii) 154 Journal of Nematology, Volume 49, No. 2, June 2017 and beet necrotic yellow vein virus (BNYVV) on single and double resistant sugar beet cultivars. European Journal of Plant Pathology 108:735–744.
    [CROSSREF]
  7. Hurlbert, S. H. 1990. Spatial distribution of the montane unicorn.Oikos 58:257–271.
    [CROSSREF]
  8. Lane, A., and Trudgill, D. 1999. Potato cyst nematode: A management guide. London: MAFF Publications.
  9. Noe, J. P., and Barker, K. R. 1985. Overestimation of yield loss of tobacco caused by the aggregated spatial pattern of Meloidogyne incognita. Journal of Nematology 17(3):245–251.
  10. Seinhorst, J. W. 1986. Measuring field populations of cyst nematodes. Pp. 105–117 in F. Lamberti and C. E. Taylor, eds. Cyst nematodes. NATO ASI series. Series A, Life sciences, vol. 121. New York: Plenum Press.
    [CROSSREF]
  11. Seinhorst, J. W. 1973. The relation between nematode distribution in a field and loss in yield at different average nematode densities. Nematologica 19:421–427.
    [CROSSREF]
  12. Schomaker, C. H., and Been, T. H. 1998. Errors due to subsampling of soil samples with Globodera rostochiensis and G. pallida, and to other laboratory procedures. Submitted to Journal of Nematology. Pp. 35–71 in T. H. Been and C. H.Schomaker, eds. Quantitative studies on the management of potato cyst nematodes (Globodera spp) in The Netherlands. PhD Thesis Wageningen Agricultural University, Wageningen,The Netherlands.
    [CROSSREF]
  13. Schomaker, C. H., and Been, T. H. 1999. A Model for infestation foci of potato cyst nematodes Globodera rostochiensis and G. pallida. Phytopathology 89(7):583–590.
    [CROSSREF]
  14. Schomaker, C. H., and Been, T. H. 2006. Plant growth and population dynamics. Pp. 276–301 in R. Perry and M. Moens, eds. Plant Nematology. Wallingford, UK: CABI.
    [CROSSREF]
  15. van Bezooijen, J. 2006. Methods and techniques for nematology.Wageningen, Netherlands: Wageningen University.

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