Biocontrol of Gray Mold Decay in Pear by Bacillus amyloliquefaciens Strain BA3 and its Effect on Postharvest Quality Parameters


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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology


ISSN: 1733-1331
eISSN: 2544-4646





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VOLUME 65 , ISSUE 2 (June 2016) > List of articles

Biocontrol of Gray Mold Decay in Pear by Bacillus amyloliquefaciens Strain BA3 and its Effect on Postharvest Quality Parameters

Hui Qu / Longyu Zhao / Fengchun Zhao / Yufang Liu / Zhengyou Yang *

Keywords : Bacillus amyloliquefaciens, Botrytis cinerea, biocontrol agents, gray mold, quality parameters

Citation Information : Polish Journal of Microbiology. Volume 65, Issue 2, Pages 171-176, DOI:

License : (CC BY-NC-ND 4.0)

Received Date : 29-October-2015 / Accepted: 30-November-2015 / Published Online: 07-June-2016



The economic losses caused by postharvest fruits diseases have attracted global attention. Traditional chemical fungicide could not meet the need of humans. In recent years, microbial agent which has begun to take the place of chemical fungicide comes into people’s vision. The aim of this paper was to investigate the potential of Bacillus amyloliquefaciens strain BA3 for its biocontrol capability on gray mold decay of pears and its effect on postharvest quality of pears. Compared with other treatments, the inhibition effect on gray mold of washed cell suspension of B. amyloliquefaciens was the best. Consequently it was utilized in subsequent experiments. Spore germination and germ tube length of Botrytis cinerea was 18.72% and 12.85 μm treated with BA3, while the control group was 62.88% and 30.44 μm. We confirmed that increase of the concentration of B. amyloliquefaciens, improved the efficacy of BA3 in controlling gray mold decay of pears. Colonization variation of BA3 in wounds of pears was recorded. To begin with, the populations of B. amyloliquefaciens increased rapidly and remained stable. On the fourth day, there was a declining trend , after that the population increased to 4 × 105 CFU/wound and remained stable. BA3 had no significant effect on mass loss, titratable acidity, firmness and total soluble solids of pears that were stored at 25°C for 7 days comparing with control group. However, the effect of B. amyloliquefaciens on ascorbic acid was significantly higher than that of the control group. Our study indicates that B. amyloliquefaciens has a potential as postharvest biocontrol agent on pears.

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AOAC. 1995. Official Methods of Analysis, 16th edn. 45.1.14. AOAC, Arlington, Virginia.


Arguelles-Arias A., M. Ongena, B. Halimi, Y. Lara, A. Brans, B. Joris and P. Fickers. 2009. Bacillus amyloliquefaciens GA1 as a source of potent antibiotics and other secondary metabolites for biocontrol of plant pathogens. Microb. Cell Fact. 8: 63–74.


Askarne L., I. Talibi, H. Boubaker, E.H. Boudyach, F. Msanda, B. Saadi and A. Ait Ben Aoumar. 2012. Use of Moroccan medicinal plant extracts as botanical fungicide against citrus blue mould. Lett. Appl. Microbiol. 56: 37–43.


Chen X.H., R. Scholz, M. Borriss, H. Junge, G. Mogel, S. Sunz and R. Borriss. 2009. Difficidin and bacilysin produced by plant-associated Bacillus amyloliquefaciens are efficient in controlling fire blight disease. J. Biotechnol. 140: 38–44.


Fan Q. and S.P. Tian. 2000. Postharvest biological control of rhizopus rot of nectarine fruits by Pichia membranefaciens. Plant Dis. 84: 1212–1216.


Feng L.Y., F.W. Wu, J. Li, Y.M. Jiang and X.W. Duan. 2011. Antifungal activities of polyhexamethylene biguanide and poly-hexamethylene guanide against the citrus sour rot pathogen Geotrichum citri-aurantii in vitro and in vivo. J. Basic Microbiol. 61: 160–164.


Hu H., F.J. Yan, C. Wilson, Q. Shen and X.D. Zheng. 2015. The ability of a cold-adapted Rhodotorula mucilaginosa strain from Tibet to control blue mold in pear fruit. 0.1007%2Fs10482-015-0593-1. 2015.10.10.


Jamalizadeh M., H.R. Etebarian, H. Aminian and A. Alizadeh. 2011. A review of mechanisms of action of biological control organisms against post-harvest fruit spoilage. Bulletin OEPP/EPPO 41: 65–71.


Li R., H. Zhang, W. Liu and X.D. Zheng. 2011. Biocontrol of postharvest gray and blue mold decay of apples with Rhodotorula mucilaginosa and possible mechanisms of action. Int. J. Food Microbiol. 146: 151–156.


Liu H.M., J.H. Guo, Y.J. Cheng, L. Luo, P. Liu, B.Q. Wang, B.X. Deng and C.A. Long. 2010. Control of gray mold of grape by Hanseniaspora uvarum and its effects on postharvest quality parameters. Ann. Microbiol. 60: 31–35.


Liu J., Y. Sui, M. Wisniewski, S. Droby and Y. Liu. 2013. Review: Utilization of antagonistic yeasts to manage postharvest fungal diseases of fruit. Int. J. Food Microbiol. 167: 153–160.


Luo S.S., B. Wan, S.H. Feng and Y.Z. Shao. 2015. Biocontrol of Postharvest Anthracnose of Mango Fruit with Debaryomyces Nepalensis and Effects on Storage Quality and Postharvest Physiology. 50-3841.13087/full. 2015.10.07.


Lutz M.C., C.A. Lopes, M.E. Rodriguez, M.C. Sosa and M.P. Sangorrín. 2013. Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pathogens of pear. Int. J. Food Microbiol. 164: 166–172.


Manso T. and C. Nunes. 2011. Metschnikowia andauensis as a new biocontrol agent of fruit postharvest diseases. Postharvest Biol. Technol. 61: 64–71.


Özden Ç. and L. Bayindirli. 2002. Effects of combinational use of controlled atmosphere, cold storage and edible coating applications on shelf life and quality attributes of green peppers. Eur. Food Res. Technol. 214: 320–326.


Sansone G., I. Rezza, V. Calvente, D. Benuzzi and M.I.S. Tosetti. 2005. Control of Botrytis cinerea strains resistant to iprodione in apple with rhodotorulic acid and yeasts. Postharvest Biol. Technol. 35: 245–251.


Sharma R., D. Singh and R. Singh. 2009. Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: a review. Biol. Control. 50: 205–221.


Spadaro D., A. Lore, A. Garibaldi and M.L. Gullino. 2013. A new strain of Metschnikowia fructicola for postharvest control of Penicillium expansum and patulin accumulation on four cultivars of apple. Postharvest Biol. Technol. 75: 1–8.


Solanki M.K., A.S. Robert, R.K. Singh, S. Kumar, A.K. Pandey, A.K. Srivastava and D.K. Arora. 2012. Characterization of mycolytic enzymes of Bacillus strains and their bio-protection role against Rhizoctonia solani in tomato. Curr. Microbiol. 65: 330–336.


Solanki M.K., R.K. Singh, S. Srivastava, S. Kumar, P.L. Kashyap and A.K. Srivastava. 2013. Characterization of antagonistic-potential of two Bacillus strains and their biocontrol activity against Rhizoctonia solani in tomato. J. Basic Microbiol. 53: 1–9.


Sugar D. and S.R. Basile. 2011. Orchard calcium and fungicide treatments mitigate effects of delayed postharvest fungicide applications for control of postharvest decay of pear fruit. Postharvest Biol. Technol. 60: 52–56.


Yang Y.N., G.F. Yao, W.Q. Yue, S.L. Zhang and J. Wu. 2015. Transcriptome profiling reveals differential gene expression in proanthocyanidin biosynthesis associated with red/green skin color mutant of pear (Pyrus communis L.). Front. Plant Sci. 6: 795.


Yu T., C. Yu, H.P. Lu, M. Zunun, F.X. Chen, T. Zhou, K. Sheng and X.D. Zheng. 2012. Effect of Cryptococcus laurentii and calcium chloride on control of Penicillium expansum and Botrytis cinerea infections in pear fruit. Biol. Control. 61:169–175.


Yu C., T. Zhou, K. Sheng, L.Z. Zeng, C.Z. Ye, T. Yu and X.D. Zheng. 2013. Effect of pyrimethanil on Cryptococcus laurentii, Rhodosporidium paludigenum, and Rhodotorula glutinis biocontrol of Penicillium expansum infection in pear fruit. Int. J. Food Microbiol. 164: 155–160.


Zhang H.Y., L. Wang, Y. Dong, S. Jiang, H.H. Zhang and X.D. Zheng. 2008a. Control of postharvest pear diseases using Rhodotorula glutinis and its effects on postharvest quality parameters. Int. J. Food Microbiol. 126: 167–171.


Zhang H.Y., L.C. Ma, L. Wang, S. Jiang, Y. Dong and X.D. Zheng. 2008b. Biocontrol of gray mold decay in peach fruit by integration of antagonistic yeast with salicylic acid and their effects on postharvest quality parameters. Biol. Control. 47: 60–65.