Identification and Localization of β-D-Glucosidase from Two Typical Oenococcus oeni Strains

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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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VOLUME 65 , ISSUE 2 (June 2016) > List of articles

Identification and Localization of β-D-Glucosidase from Two Typical Oenococcus oeni Strains

Yahui Li / Yanhong Ma * / Kaihong Huang / Hongzhi Zhang

Keywords : Oenococcus oeni, β-D-glucosidase, βG cloned, gene localization

Citation Information : Polish Journal of Microbiology. Volume 65, Issue 2, Pages 209-213, DOI: https://doi.org/10.5604/17331331.1204481

License : (CC BY-NC-ND 4.0)

Received Date : 02-March-2015 / Accepted: 06-September-2015 / Published Online: 07-June-2016

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ABSTRACT

β-D-glucosidase (βG) gene from Oenococcus oeni SD-2a and 31MBR was cloned, sequenced and analyzed, also intracellular βG of the two strains was further localized. The results showed that βG gene of the two strains was in high homology (> 99%) to reported βG gene, con­firming both strains possess βG activity at the molecular level. Intracellular βG of SD-2a is a mainly soluble protein, existing mostly in the cytoplasm and to some extent in the periplasm. While for 31MBR, intracellular βG is mainly insoluble protein existing in the cytoplasmic membrane. This study provides basic information for further study of the metabolic mechanism of βG from O. oeni SD-2a and 31MBR.

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