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  • Journal Of Nematology

 

research-article | 30-November-2020

Cultivation of Caenorhabditis elegans on new cheap monoxenic media without peptone

The nematode Caenorhabditis elegans is a lab workhorse and one of the best studied animals at the molecular and genetic levels. While C. elegans can be found in rotting fruits and leaves (Barriere and Felix, 2006; Felix and Braendle, 2010; Felix et al., 2013; Kiontke et al., 2011; Petersen et al., 2014; Samuel et al., 2016; Schulenburg and Felix, 2017), its distribution and ecology are poorly understood (Frezal and Felix, 2015; Kiontke et al., 2011). Even less natural history is known for other

Tho Son Le, T. T. Hang Nguyen, Bui Thi Mai Huong, H. Gam Nguyen, B. Hong Ha, Van Sang Nguyen, Minh Hung Nguyen, Huy-Hoang Nguyen, John Wang

Journal of Nematology, Volume 53 , 1–23

Research Article | 26-September-2018

Modeling Host-Microbiome Interactions in Caenorhabditis elegans

The microbiome influences host processes including nutritional availability, development, immunity, and behavioral responses. Caenorhabditis elegans is a powerful model to study molecular mechanisms of host–microbial interactions. Recent efforts have been made to profile the natural microbiome of C. elegans, laying a foundation for mechanistic studies of host–microbiome interactions in this genetically tractable model system. Studies using single-species microbes, multi-microbial systems, and

TEKLU K. GERBABA, LUKE GREEN-HARRISON, ANDRE G. BURET

Journal of Nematology, Volume 49 , ISSUE 4, 348–356

Research Article | 31-May-2018

Chemotaxis of Caenorhabditis elegans toward volatile organic compounds from Stropharia rugosoannulata induced by amino acids

A variety of natural substances including both volatile organic compounds and water-soluble compounds play a significant role in the chemotactic behaviors of the model nematode Caenorhabditis elegans. We observed chemotactic behaviors of C. elegans with respect to response to attractants produced by nematode parasitic fungus Stropharia rugosoannulata, which were partially induced by specific amino acids. The results of gas chromatography-mass spectrometer analysis suggested that 1-octen-3-ol

Jing Bian, Haili Zhang, Shuai Meng, Yajun Liu

Journal of Nematology, Volume 50 , ISSUE 1, 3–8

Research Article | 26-September-2018

A Model for Evolutionary Ecology of Disease: The Case for Caenorhabditis Nematodes and Their Natural Parasites

Many of the outstanding questions in disease ecology and evolution call for combining observation of natural host–parasite populations with experimental dissection of interactions in the field and the laboratory. The “rewilding” of model systems holds great promise for this endeavor. Here, we highlight the potential for development of the nematode Caenorhabditis elegans and its close relatives as a model for the study of disease ecology and evolution. This powerful laboratory model was

AMANDA K. GIBSON, LEVI T. MORRAN

Journal of Nematology, Volume 49 , ISSUE 4, 357–372

research-article | 06-November-2020

Competitive fitness analysis using Convolutional Neural Network

interest (e.g., evolved vs. ancestral populations in experimental evolution studies) is often impractical because of the difficulty, or even impossibility, in distinguishing their progeny in the population. Thus, relative fitness is often assessed by competition between a population of interest and a common ‘tester’ strain with distinct morphology (reviewed in Teotónio et al., 2017). Caenorhabditis nematodes are increasingly commonly used models in evolutionary and ecological studies, which enable a

Joanna K. Palka, Krzysztof Fiok, Weronika Antoł, Zofia M. Prokop

Journal of Nematology, Volume 52 , 1–15

Research Article | 31-May-2018

Postembryonic Ventral Nerve Cord Development and Gonad Migration in Steinernema carpocapsae

the dauer stage of Caenorhabditis elegans. Here, we used in vitro cultures of S. carpocapsae to examine the early development of the ventral nerve cord (VNC). Similar to C. elegans, S. carpocapsae hatches as a J1 with a VNC containing only a fraction of the neurons found in later developmental stages. During J1 development, S. carpocapsae adds additional cells to the VNC to establish the complete set of neurons. During our examination of the VNC, we also noted variable gonad arm development among

Hung Xuan Bui, Nathan E. Schroeder

Journal of Nematology, Volume 50 , ISSUE 1, 27–32

Article | 21-July-2017

Assessment of Globodera pallida RNA Extracted from Solanum Roots

The introduction of high-throughput sequencing technologies has made transcriptome analyses of plant–pathogen interactions almost routine. Nevertheless, it is still challenging to obtain RNA from populations made up of two species. An RNA extraction method that worked well on free-living Caenorhabditis elegans failed when applied to isolated Globodera pallida J2 larva. Furthermore, alternative protocols that extracted RNA from free-living J2 larva produced less satisfactory

N. CAROL CASAVANT, JOSEPH C. KUHL, FANGMING XIAO, ALLAN B. CAPLAN, LOUISE-MARIE DANDURAND

Journal of Nematology, Volume 49 , ISSUE 1, 12–20

research-article | 30-November-2019

Detoxification-related gene expression accompanies anhydrobiosis in the foliar nematode (Aphelenchoides fragariae)

Zhen Fu, Paula Agudelo, Christina E. Wells

Journal of Nematology, Volume 52 , 1–12

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