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Article | 14-October-2020

False reactivity in GTI Pak Plus® ELISA kits due to the presence of anti-mouse antibody in patients' samples

The development of commercially available ELISA kits (GTI, Inc., Waukesha,WI) that use antigens adhered to microtiter plate wells by the use of mouse monoclonal antibodies made it possible for hospital transfusion service laboratories to test for platelet- and/or HLA-specific antibodies without reliance on reference laboratories. However, human anti-mouse antibodies (HAMAs) may cause false reactions in ELISAs. We designed a study to determine the impact of HAMAs on these ELISAs. Samples from

Miriam Fogg Leach, James P. AuBuchon

Immunohematology, Volume 19 , ISSUE 4, 112–116

Article | 09-November-2020

Semiautomation of platelet HPA-1a phenotyping by SPRCA and ELISA

An enzyme-linked immunosorbent assay (ELISA) and solid phase red cell adherence assay (SPRCA) were assessed for platelet HPA-1a typing in U well microplates. Both methods were partially automated by the use of the Tecan RSP 8051ID robotic sampler and the SLT 400 ATC plate reader with Soft2000 software. Pretreatment of the adherent platelets with chloroquine diphosphate or citric acid enabled anti-HPA-1a, even when contaminated with HLA class 1 antibodies, to be used for typing. Of 675 antenatal

Lionel A. Mohabir, Lynne Porter

Immunohematology, Volume 13 , ISSUE 2, 44–48

Original Paper | 09-March-2018

Comparison of Methods Used for the Diagnosis of Epstein-Barr Virus Infections in Children

The accurate diagnosis of Epstein-Barr virus (EBV) infections is important, as many other infectious agents or diseases can cause similar symptoms. In this study, sera of pediatric patients who were suspected to have an EBV infection, were sent to Eskisehir Osmangazi University Faculty of Medicine, Department of Clinical Microbiology, and investigated by IFA, ELISA, immunoblotting and Real-time PCR. The performances of these tests were compared with IFA. The rates of agreement between ELISA and

Nilgun Kasifoglu, Semra Oz, Ener Cagri Dinleyici, Tercan Us, Ozcan Bor, Gul Durmaz, Yurdanur Akgun

Polish Journal of Microbiology, Volume 67 , ISSUE 1, 81–88

original-paper | 27-March-2019

Clinical Interpretation of Detection of IgM Anti-Brucella Antibody in the Absence of IgG and Vice Versa; a Diagnostic Challenge for Clinicians

examination has led to consider other diagnostic techniques more useful in the diagnostic workup of brucellosis. Serologically, ELISA is the most popular and widely used diagnostic assay. Brucella-specific IgM antibodies are produced in the first week after the disease onset, reaching a maximum after two months. On the other hand, IgG antibodies are detected after the second week of infection, attaining a peak level of six to eight weeks later. While IgG response coincides more closely with the clinical


Polish Journal of Microbiology, Volume 68 , ISSUE 1, 51–57

Article | 18-October-2020

Quantitation of red cell-bound immunoglobulins and complement in lymphoma patients

Quantitative ELISA may be useful for determining the amount of red blood cell (RBC)-associated immunoglobulins (Igs) in patients with autoimmune hemolytic anemia (AIHA). In idiopathic AIHA, there is about 20 times more RBC-associated IgG and complement than in normal persons. In patients with low-grade lymphomas (particularly, B-CLL and splenic marginal zone lymphoma) autoimmune hemolysis is a component of their anemia. In highgrade malignant lymphomas (i.e, diffuse large B-cell lymphoma and

M. Podberezin, A. Levina, L. Romanova, O. Margolin, O. Nasibov, A.V. Pivnik

Immunohematology, Volume 16 , ISSUE 4, 147–153

original-paper | 01-May-2021

Screening of Human Immunodeficiency Virus (HIV) among Newly Diagnosed Tuberculosis Patients in Eastern Sudan

collected from each patient, then serum samples were separated (Tognon et al. 2020) and investigated for HIV antibodies by using fourth-generation enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions. Data was analyzed by IBM SPSS Statistics for Windows, Version 20 (Armonk, NY: IBM Corp) and iNZight (The University of Auckland New Zealand). Chi-square test was used to test the p-value, and it was deemed significant if it was less than 0.05. Ethical approval for this


Polish Journal of Microbiology, Volume 70 , ISSUE 2, 201–206

Article | 10-April-2021

An automated approach to determine antibody endpoint titers for COVID-19 by an enzyme-linked immunosorbent assay

immunosorbent assay (ELISA) approach with a key antigen as a surrogate for functional assays also allows direct examination of the antibody isotype, a feature of antibodies that dictates antibody localization and therefore a function not typically assessed in neutralization assays. This feature is especially important when considering that, while most assays examine total or immunoglobulin (Ig)G-specific antibody levels against SARS-CoV-2, few examine IgA, the isotype with the unique ability to be

A.D. Ho, H. Verkerke, J.W. Allen, B.J. Saeedi, D. Boyer, J. Owens, S. Shin, M. Horwath, K. Patel, A. Paul, S.-C. Wu, S. Chonat, P. Zerra, C. Lough, J.D. Roback, A. Neish, C.D. Josephson, C.M. Arthur, S.R. Stowell

Immunohematology, Volume 37 , ISSUE 1, 33–43

Article | 27-April-2020

Is there a relationship between anti-HPA-1a concentration and severity of neonatal alloimmune thrombocytopenia?

There is uncertainty about the relationship between anti-HPA-1a levels and severity of neonatal alloimmune thrombocytopenia (NAIT). To investigate this relationship further,the concentration of anti-HPA-1a in HPA-1b homozygous women was determined,using a newly developed quantitative ELISA that uses purified anti-HPA-1a to obtain a standard curve. Seventy-eight samples collected from 22 HPA-1b homozygous pregnant women at various stages of pregnancy were tested. These included five women who

Hagop Bessos, Marc L. Turner, Stanislaw J. Urbaniak

Immunohematology, Volume 21 , ISSUE 3, 102–108

Article | 06-December-2020

Cytomegalovirus antibody screening on the Olympus PK7100

(LAT). There were four discrepant results in the 1,495 donor plasmas tested by the two methods. Two of the three samples that were positive by the LAT and negative by the OHA test were also negative with the IMx enzyme-linked immunosorbent assay (ELISA) and complement fixation test (CFT). The OHA failed to detect antibodies to CMV in a sample that was positive by the IMx ELISA, CFT, and LAT. The IMx ELISA, which was regarded as the arbiter of true positivity, yielded a sensitivity and specificity

LioneI A. Mohabir

Immunohematology, Volume 8 , ISSUE 2, 41–43

Article | 09-November-2020

Measurement of red blood cell-bound C3b and C3d using an enzyme-linked direct antiglobulin test

J.D. Bellamy, D.J. Booker, N.T. James, R. Stamps, R.J. Sokol

Immunohematology, Volume 13 , ISSUE 4, 123–131

Article | 03-November-2020

Use of the MAIEA assay to demonstrate that Fy3 is on the same glycoprotein as Fy6, Fya, and Fyb

Jaw-Lin Tzeng, Roger Dodd, Delores Mallory

Immunohematology, Volume 14 , ISSUE 3, 113–116

Article | 06-July-2017


Olusanya Elisa Olubusoye, Grace Oluwatoyin Korter, Afees Adebare Salisu

Statistics in Transition New Series, Volume 17 , ISSUE 4, 659–670

Short Communication | 06-February-2018

In vivo stimulation of locus coeruleus: effects on amygdala subnuclei

Elisa Rodríguez-Ortega, Fernando Cañadas, Francisca Carvajal, Diana Cardona

Acta Neurobiologiae Experimentalis, Volume 77 , ISSUE 3, 261–268

Short Communication | 04-December-2017

Seroprevalence of Rubella and Cytomegalia in Young Women from Biała Podlaska District

The aim of this study was to analyze the seroprevalence of rubella and cytomegalia among young women. The study included 175 healthy women from the Biała Podlaska District, aged 16 to 35 years. Anti-rubella and anti-CMV IgG were determined by ELISA. 172 (98.3%) study subjects tested positive for rubella antibodies, 1 (0.6%) was seroindeterminate and 2 (1.1%) were seronegative. CMV antibodies were detected in 119 (68.0%) participants; the series included also 1 (0.6%) seroindeterminate and 55

Dorota Plewik, Małgorzata Tokarska-Rodak, Justyna Paszkiewicz, Adam Szepeluk

Polish Journal of Microbiology, Volume 66 , ISSUE 4, 543–545

original-paper | 28-June-2019

The Joint Effect of pH Gradient and Glucose Feeding on the Growth Kinetics of Lactococcus lactis CECT 539 in Glucose-Limited Fed-Batch Cultures


Polish Journal of Microbiology, Volume 68 , ISSUE 2, 269–280

Review | 18-May-2020

Review: IgA anaphylactic transfusion reactions. Part I. Laboratory diagnosis, incidence,and supply of IgAdeficient products

Ralph R. Vassallo

Immunohematology, Volume 20 , ISSUE 4, 226–233

Research paper | 06-February-2018

Assessment of type 1 and type 3 deiodinase expression levels in depressive disorders

. We aimed to investigate the levels of DIO1 and DIO3 in the patients suffering from recurrent depressive disorders (rDD). Data collected from 91 rDD patients and 105 healthy controls were analyzed. The diagnoses were made based on the ICD-10 criteria (F33.0–F33.8). The expression levels of DIO1 and DIO3 were estimated using the polymerase chain reaction method and the enzyme-linked immunosorbent assay (ELISA). The expression of DIO1 on mRNA/protein levels in the rDD patients was reduced in

Elżbieta Gałecka, Anna Kumor-Kisielewska, Agata Orzechowska, Michael Maes, Paweł Górski, Janusz Szemraj

Acta Neurobiologiae Experimentalis, Volume 77 , ISSUE 3, 225–235

Article | 31-December-2019


ten sposób diagnostyki zalecany jest przez Polskie Towarzystwo Epidemiologów i Lekarzy Chorób Zakaźnych od 2007 roku. Pierwszym etapem tego podejścia diagnostycznego jest czuły test immunoenzymatyczny (ELISA, enzyme-linked immunosorbent assay). Jeżeli test ELISA da wynik dodatni lub wątpliwy jako test drugiego stopnia (potwierdzający) stosuje się Western blot (WB) [2, 45]. Testy ELISA ze względu na swoją wysoką czułość pełnią rolę testów przesiewowych. Oparte są one głównie na całkowitych lizatach

Weronika Grąźlewska, Lucyna Holec-Gąsior

Postępy Mikrobiologii - Advancements of Microbiology, Volume 58 , ISSUE 4, 399–413

research-article | 15-October-2020

Antistress and antidepressant properties of dapoxetine and vortioxetine

day (30 day of the experiment) after last forced swimming test protocol all of the rats (24 NS and 24 RS rats) were sacrificed by decapitation, and trunk blood was collected to establish corticosterone level in each group of animals (treated by vehicle or VOR, DAP, VOR+DAP). Blood was collected immediately to plastic tubes containing 10% ethylenediaminetetraacetate (EDTA). Due to the sensitivity of the Corticosterone ELISA kit, the rats did not receive any anesthetic substances that could falsify

Piotr Ratajczak, Krzysztof Kus, Małgorzata Zielińska-Przyjemska, Beata Skórczewska, Tomasz Zaprutko, Dorota Kopciuch, Anna Paczkowska, Elżbieta Nowakowska

Acta Neurobiologiae Experimentalis, Volume 80 , ISSUE 3, 217–224

original-paper | 09-April-2019

Dengue Outbreaks in Khyber Pakhtunkhwa (KPK), Pakistan in 2017: An Integrated Disease Surveillance and Response System (IDSRS)-Based Report

were investigated for various signs and symptoms e.g. fever, body aches, nausea, vomiting, diarrhea, gum bleeding and bleeding of the nose, skin rashes, animals contact history, and platelet count at admission. ELISA. The data collected was obtained from medical records. About 120 948 patients were reviewed retrospectively for demographic and clinical as well as laboratory data. The patients’ serum was investigated for the presence of non-structural protein-1 (NS1) antigen in order to identify DENV


Polish Journal of Microbiology, Volume 68 , ISSUE 1, 115–119

original-paper | 04-June-2020

Performance Evaluation of Different Commercial Serological Kits for Diagnosis of Acute Hepatitis E Viral Infection

hepatitis E cases mainly depends on the serological detection of anti-HEV antibodies (Dreier and Juhl 2014). However, equivalence, sensitivity, and specificity in the results of the HEV Enzyme-linked Immunosorbent Assay (ELISA) kits tend to differ between manufacturers, leading to discrepancies in the rates of anti-HEV antibodies among different populations (Herremans et al. 2007; Drobeniuc et al. 2010), together with the HEV genome heterogeneity, and the different antigenic structure of HEV proteins


Polish Journal of Microbiology, Volume 69 , ISSUE 2, 217–222

Case report | 14-October-2020

Neonatal alloimmune thrombocytopenia due to HPA-3a antibodies: a case report

commerical antigencapture ELISA (GTI-PakPlus kit®). Anti-HPA-3a antibodies, while weakly reactive in the monoclonal antibody immobilization of platelet antigens (MAIPA) assay in the immediate postpartum serum, were readily detectable using this assay in a sample taken 4 weeks later. Genotyping for human platelet antigens (HPA) 1–5 by the polymerase chain reaction technique with sequence-specific primers (PCR-SSP) revealed the infant’s platelet genotype to be HPA-1a/1a, 3a/3b, while that

A. Davoren, G. Smith, G. Lucas, S. Rodgers, P. O’Donoghue, J. Crowley, C.A. Barnes, J. McKiernan

Immunohematology, Volume 18 , ISSUE 2, 33–36

research-article | 26-March-2021

Aminoguanidine ameliorates ovariectomy-induced neuronal deficits in rats by inhibiting AGE-mediated Aβ production

tested for two consecutive days; the correct escape rate was determined by the number of passes into the unsafe zones within ten repetitions, and the escape latency was determined by the time to the first pass into the safe zone from an unsafe zone. ELISA After the behavioural evaluation, all survived animals were humanely sacrificed at the 21th week of the experiment by an intraperitoneal injection of 5% chloral hydrate (0.4 mL/kg). The urine was collected and blood was sampled from the abdominal

Dan Di Zhang, Yan Gang Wang, Chun Yan Liu, Ze Hou Wang, Yue Fen Wang

Acta Neurobiologiae Experimentalis, Volume 81 , ISSUE 1, 10–20

Review | 09-October-2019

Rare donor program in Italy

Cinzia Paccapelo, Francesca Truglio, Maria Antonietta Villa, Nicoletta Revelli, Maria Cristina Manera, Elisa Erba, Maurizio Marconi

Immunohematology, Volume 32 , ISSUE 2, 47–48

research-article | 06-April-2020

Hindbrain metabolic deficiency regulates ventromedial hypothalamic nucleus glycogen metabolism and glucose-regulatory signaling

hypothesis that metabolic deficit-propelled NE input to the VMN stimulates GABA signaling. Here, groups of adult male rats were injected into the caudal fourth ventricle (CV4) with the monocarboxylate transporter inhibitor alpha-cyano-4-hydroxycinnamic acid (4CIN) or vehicle alone (Patil and Briski, 2005). Micropunch-dissected VMN tissue was analyzed by Western blotting and ELISA methods to determine 4CIN effects on VMN GAD65/67 protein expression and NE activity, respectively. A subset of subjects in

Karen P. Briski, Santosh K. Mandal

Acta Neurobiologiae Experimentalis, Volume 80 , ISSUE 1, 57–65

Article | 14-October-2020

Neonatal alloimmune thrombocytopenia due to anti-HPA-2b (anti-Koa)

of the hematoma was performed after transfusion. Testing with a solid phase ELISA revealed reactivity against GP1b/IX. MAIPA testing after platelet treatment with the protease inhibitor leupeptin demonstrated the presence of anti-HPA-2b. On PCR-SSP the mother was HPA-2a homozygous, the father was HPA-2a/2b. Antibodies against the HPA-2b antigen located on the GP1b/IX complex have been reported in rare cases of NAIT. Testing is complicated by proteolytic degradation of the antigen-bearing fragment

Mindy Goldman, Élise Trudel, Samir Khalife, Gwendoline M. Spurll

Immunohematology, Volume 19 , ISSUE 2, 43–46

Article | 20-December-2020

A simple method for inhibiting ABO antibodies in sera used for platelet crossmatching

by Neutr-AB when tested against group A or B platelets by solid-phase ELISA. The amount of A antigen on platelets and the levels of anti-A in group B or O sera can vary considerably, so we investigated the extent of the problem in platelet crossmatching. Sixty percent of 20 random group O sera reacted with platelets from a donor, who was selected because of the strong A antigen present on the platelets. Eighty-six percent of randomly selected group A platelets reacted with a group O serum

Nina Postoway, George Garratty

Immunohematology, Volume 6 , ISSUE 3, 68–70

Research Article | 18-January-2019

A collection of tributes to Linton C. Freeman

Russ Bernard, Elisa Bienenstock, Steve Borgatti, Ulrik Brandes, Ron Burt, Carter Butts, Pat Doreian, Tom Fararo, Katie Faust, Jeff Johnson, Alaina Michaelson Kanfer, David Krackhardt, John Skvoretz, Barry Wellman

Connections, Volume 38 , ISSUE 1, 1–16

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