• Select Article Type
  • Abstract Supplements
  • Blood Group Review
  • Call to Arms
  • Hypothesis
  • In Memoriam
  • Interview
  • Introduction
  • Letter to the Editor
  • Short Report
  • abstract
  • Abstracts
  • Article
  • book-review
  • case-report
  • case-study
  • Clinical Practice
  • Commentary
  • Conference Presentation
  • conference-report
  • congress-report
  • Correction
  • critical-appraisal
  • Editorial
  • Editorial Comment
  • Erratum
  • Events
  • Letter
  • Letter to Editor
  • mini-review
  • minireview
  • News
  • non-scientific
  • Obituary
  • original-paper
  • original-report
  • Original Research
  • Pictorial Review
  • Position Paper
  • Practice Report
  • Preface
  • Preliminary report
  • Product Review
  • rapid-communication
  • Report
  • research-article
  • Research Communicate
  • research-paper
  • Research Report
  • Review
  • review -article
  • review-article
  • review-paper
  • Review Paper
  • Sampling Methods
  • Scientific Commentary
  • short-communication
  • short-report
  • Student Essay
  • Varia
  • Welome
  • Select Journal
  • Polish Journal Of Microbiology
  • Journal Of Nematology
  • Immunohematology
  • Acta Neurobiologiae Experimentalis


Article | 09-November-2020

A modified PCR-RFLP genotyping method demonstrates the presence of the HPA-4b platelet alloantigen in a North American Indian population

the HPA-4 antigen system does not involve a common naturally occurring restriction enzyme site. This paper describes a new genotyping method for HPA-4 (polymerase chain reaction–restriction fragment length polymorphism [PCR-RFLP]) that involves restriction enzyme digestion of PCR-amplified genomic DNA using a modified PCR primer to create an artificial TaqI restriction site that is present in the HPA-4a but not in the HPA-4b DNA sequence. The HPA-4 PCR-RFLP method was validated by testing a

Alexander P. Reiner, Gayle Teramura

Immunohematology, Volume 13 , ISSUE 2, 37–43

Article | 14-October-2020

DNA analysis for donor screening of Dombrock blood group antigens

PCR-RFLP assay as an alternative to traditional hemagglutination for typing donor blood for Dombrock. Primers were designed to amplify the region of DO containing the 793A>G polymorphism. DNA samples from blood donors were amplified and subjected to RFLP analysis. A total of 613 samples were tested for the Dombrock polymorphism (793 A>G) by PCRRFLP. PCR-RFLP can be used to screen for Do(a–) or Do(b–) donors. This approach overcomes the scarcity of the reagents required for

Jill R. Storry, Connie M. Westhoff, Dalisay Charles-Pierre, Maria Rios, Kim Hue-Roye, Sunitha Vege, Sandra Nance, Marion E. Reid

Immunohematology, Volume 19 , ISSUE 3, 73–76

Research paper | 31-July-2017

Analysis of methionine synthase (rs1805087) gene polymorphism in autism patients in Northern Iran

this study was to analyze the association of MTR A2756G gene polymorphism (rs1805087) and the risk of autism in a population in northern Iran. The prevalence of MTR A2756G polymorphism was determined in 108 children with autism and 130 controls in northern Iran. Genotypes and allele frequencies were determined in patients and controls by polymerase chain reaction‑restriction fragment length polymorphism (PCRRFLP). The prevalence of genotype frequencies of AA, AG and GG in autistic children were

Rosa Haghiri, Farhad Mashayekhi, Elham Bidabadi, Zivar Salehi

Acta Neurobiologiae Experimentalis, Volume 76 , ISSUE 4, 318–323

Article | 16-February-2021

Comparison of ABO genotyping methods: a study of two low-resolution polymerase chain reaction assays in a clinical testing laboratory

the most likely result is A1 but that rare alleles cannot be ruled out. PCR-RFLP interrogating of nucleotides c.261G/delG, c.467C/T, c.703G/A, and c.1096G/A, designed to discriminate between the common alleles A1, A2, B, O1, and O2, was performed as described by Olsson and Chester.5 In a subset of cases, Sanger sequencing of ABO exons 1–7 was performed (Grifols Immunohematology Center, Grifols Diagnostic Solutions, San Marcos, TX). In one case, PCR products of ABO gene fragments were cloned into

J.A. Keller, T. Horn, S. Scholz, S. Koenig, M.A. Keller

Immunohematology, Volume 35 , ISSUE 4, 149–153

Report | 25-March-2020

Molecular studies of DO alleles reveal that JO is more prevalent than HY in Brazil, whereas HY  is more prevalent in New York

Because of the scarcity of anti-Hy and anti-Joa, hemagglutination typing for the Dombrock blood group system antigens, Hy and Joa, is not feasible.  The molecular bases associated with these antigens have been determined, making it possible to distinguish HY and JO from wild-type DO.  This provides a tool to predict the probable phenotype of patients and to screen for antigen-negative donors.  PCR-RFLP assays and a microchip assay were used to determine the frequency of HY and JO

Lilian Castilho, Wilson Baleotti, Edith Tossas, Kim Hue-Roye, Karina R. Ribeiro, Christine Lomas-Francis, Daisy Charles-Pierre, Marion E. Reid

Immunohematology, Volume 24 , ISSUE 4, 135–137

Article | 14-October-2020

Studies on the Dombrock blood group system in non-human primates

phenotypes have been determined. The purpose of this study was to perform DNA-based assays on the DO homolog in non-human primates to determine the degree of conservation in the DO gene. Murine MoAbs to Dombrock protein were developed by standard hybridoma technologies and used to test RBCs from non-human primates by hemagglutination. PCR-RFLP analysis for the six singlenucleotide polymorphisms (SNPs) that have been defined in human alleles were performed on DNA extracted from fresh or frozen blood

Cristina Mogos, Alissa Schawalder, Gregory R. Halverson, Marion E. Reid

Immunohematology, Volume 19 , ISSUE 3, 77–82

original-paper | 05-August-2020

Yeasts Associated with Various Amazonian Native Fruits


Polish Journal of Microbiology, Volume 69 , ISSUE 3, 251–261

Article | 14-October-2020

Easy method for determining the frequency of O1 and O2 alleles in Brazilian blood donors by PCR-RFLP analysis

Ana C. Batissoco, Marcia C.Z. Novaretti, Valdecir J. Bueno, Pedro E. Dorlhiac-Llacer, Dalton A.F. Chamone

Immunohematology, Volume 17 , ISSUE 4, 111–116

Article | 16-November-2020

ABO genotyping - identification of O1, O1*, and O2 alleles using the polymerase chain reaction– sequence specific oligonucleotide (PCR-SSO) technique

ABO polymorphism at the gene level has been investigated by molecular methods, predominantly sequencing and restriction fragment length polymorphism (RFLP). We describe the application of the polymerase chain reaction–sequence specific oligonucleotide (PCRSSO) method, which is considered to be more versatile for large sample numbers, compared with conventional ABO genotyping by PCR-RFLP. PCR-SSO, while maintaining accurate and reliable results, reduces costs and labor. A population of 155

Nicole A. Mifsud, Albert P. Haddad, Jennifer A. Condon, Rosemary L. Sparrow

Immunohematology, Volume 12 , ISSUE 4, 149–153

Article | 16-November-2020

ABO genotyping by polymerase chain reaction-restriction fragment length polymorphism

Genotyping enables the identification of both maternally and paternally derived alleles. A number of protocols have been described for the genotyping of the ABO blood group system. Generally, these methods have a number of disadvantages including the use of hazardous reagents, being technically demanding, and the excessive use of materials. In this study, a relatively simple polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method is described. Four different

Nicole A. Mifsud, Albert P. Haddad, Jennifer A. Condon, Rosemary L. Sparrow

Immunohematology, Volume 12 , ISSUE 4, 143–148

Original Paper | 26-August-2016

Characterization of Rhizobial Bacteria Nodulating Astragalus corrugatus and Hippocrepis areolata in Tunisian Arid Soils

Fifty seven bacterial isolates from root nodules of two spontaneous legumes (Astragalus corrugatus and Hippocrepis areolata) growing in the arid areas of Tunisia were characterized by phenotypic features, 16S rDNA PCR-RFLP and 16S rRNA gene sequencing. Phenotypically, our results indicate that A. corrugatus and H. areolata isolates showed heterogenic responses to the different phenotypic features. All isolates were acid producers, fast growers and all of them used different compounds as sole

Mosbah Mahdhi, Nadia Houidheg, Neji Mahmoudi, Abdelhakim Msaadek, Mokhtar Rejili, Mohamed Mars

Polish Journal of Microbiology, Volume 65 , ISSUE 3, 331–339

Report | 12-March-2020

RHCE*ceAR encodes a partial c (RH4) antigen

The Rh blood group system is highly complex both in the number of discrete antigens and in the existence of partial antigens, especially D and e.  Recently, several partial c antigens have been reported. Here we report findings on an African American man with sickle cell disease whose RBCs typed C+c+ and whose plasma contained anti-c. Hemagglutination tests, DNA extraction, PCR-RFLP, reticulocyte RNA isolation, RT-PCR cDNA analyses, cloning, and sequencing were performed by standard

Marion E. Reid, Christine Halter Hipsky, Christine Lomas-Francis, Akiko Fuchisawa

Immunohematology, Volume 26 , ISSUE 2, 57–59

Article | 21-April-2020

Analysis of SERF in Thai blood donors

DAF. This study reports on PCR-RFLP analysis of the SERF allele with BstNI restriction endonuclease on more than one thousand Thai blood donor samples. One new donor homozygous (647T) and 21 donors heterozygous (647C/T) for the SERF allele were found. Among this cohort of random Thai blood donors,the SERF allele frequency was 1.1 percent. Thus, like other alleles in the Cromer blood group system, SERF is found in a certain ethnic group.

Poonsub Palacajornsuk, Kim Hue-Roye, Oytip Nathalang, Srisurang Tantimavanich, Sasitorn Bejrachandra, Marion Reid

Immunohematology, Volume 21 , ISSUE 2, 66–69

Article | 21-July-2017

Mitochondrial Haplotype-based Identification of Root-knot Nematodes (Meloidogyne spp.) on Cut Foliage Crops in Florida


Journal of Nematology, Volume 48 , ISSUE 3, 193–202

Report | 16-March-2020

The polymorphism nt 76 in exon 2 of SC is more frequent in Whites than in Blacks

African American donors was tested by polymerase chain reaction (PCR)restriction fragment length polymorphism (RFLP) using the restriction enzyme NlaIII. In selected samples, sequencing of exon 2 was performed. PCR-RFLP results for samples from 100 donors (mostly Caucasian) and 100 African American donors (400 alleles) showed the nucleotide 76T variant had a prevalence of 25 percent in Whites and 5 percent in African Americans. In 11 samples (2 C/C, 3 C/T, and 6 T/T) sequencing of exon 2 confirmed the

Akiko Fuchisawa, Christine Lomas-Francis, Kim Hue-Roye, Marion E. Reid

Immunohematology, Volume 25 , ISSUE 1, 18–19

research-article | 30-November-2020

First report of morphological and molecular characterization of Moroccan populations of Globodera pallida

is the most reliable method for the detection of PCN species (Powers, 2004). PCR with specific primers used in single or multiplex reactions and PCR-RFLP (Restriction Fragment Length Polymorphisms) are among the tests developed to identify Globodera species (Fullaondo et al., 1999; Blok et al., 1998; Bulman and Marshall, 1997; Vejl et al., 2002). The present study was conducted in different regions known to be the main potato production areas in Morocco. The main objective was to carry out a

A. Hajjaji, R. Ait Mhand, N. Rhallabi, F. Mellouki

Journal of Nematology, Volume 53 , 1–8

Article | 01-April-2020

An alloantibody to a highprevalence MNS antigen in a person with a GP.JL/Mk phenotype

. Immunoblotting showed the presence of monomer and dimer forms of a GP(A-B) hybrid and an absence of GPA and GPB. Sequencing of DNA and PCR-RFLP using the restriction enzyme RsaI confirmed the presence of a hybrid GYP(AB). The patient’s antibody was determined to be anti-EnaFR. She is the first person reported with the GP.JL phenotype associated with a deletion of GYPA and GYPB in trans to GYP.JL.

John Ratliff, Susan Veneman, Joan Ward, Christine Lomas-Francis, Kim Hue-Roye, Randall W. Velliquette, Laima Sausais, Twilla Maldonado, Janet Miyamoto, Yolanda Martin, David Slater, Marion E. Reid

Immunohematology, Volume 23 , ISSUE 4, 146–149

Case report | 14-October-2020

Moderate hemolytic disease of the newborn (HDN) due to anti-Rh17 produced by a black female with an e variant phenotype

cause mild to fatal HDN. We report an example of anti-Rh17 produced by a black female with an e variant RBC phenotype that caused moderate HDN. A panel of seven monoclonal anti-e demonstrated her RBCs carried a variant e antigen, and her genotype was RHD, RHce by PCR-RFLP analysis. Amniotic fluid with ΔOD450 values from 30 to 35 weeks’ gestation predicted moderate HDN probability by the Liley method. At 38+ weeks, a viable 3165 g female infant was delivered. The infant’s direct

Marla C. Brumit, Gary E. Carnahan, James R. Stubbs, Jill R. Storry, Marion E. Reid

Immunohematology, Volume 18 , ISSUE 2, 40–42

Case report | 25-March-2020

RHD deletion in a patient with chronic myeloid leukemia

primers designed to amplify RHD exons 3, 4, or 7.  Two assays that detect the hybrid Rhesus box showed deletion of RHD.  Amplification of RHCE in the patient’s DNA was as efficient as that of control samples, and multiplex and PCR-RFLP assays predicted her RBCs would be C–E–c+e+.  Based on finding a hybrid Rhesus box and absence of D-specific exons, we conclude that DNA from the patient’s WBCs carries a deleted RHD.  This explains the molecular mechanism

Ann Murdock, Deborah Assip, Kim Hue-Roye, Christine Lomas-Francis, Zong Hu, Sunitha Vege, Connie M. Westhoff, Marion E. Reid

Immunohematology, Volume 24 , ISSUE 4, 160–164

Report | 01-December-2019

SC*994C>T causes the Scnull phenotype in Pacific Islanders and successful transfusion of Sc3+ blood to a patient with anti-Sc3  

African American donors (n = 99) were tested using the Tsp45I PCR-RFLP assay; all gave a banding pattern that was consistent with the SC*994C/C consensus sequence. In all five samples, our analyses showed homozygosity for the nonsense nucleotide change SC*994C>T in an allele carrying the nucleotide associated with Sc1. Further investigation determined that one of the probands reported previously with the SC*994C>T change was from the Marshall Islands (which form part of the Micronesian Pacific

Marion E. Reid, Kim Hue-Roye, Randall W. Velliquette, Kathleen Larimore, Sue Moscarelli, Nicolas Ohswaldt, Christine Lomas-Francis

Immunohematology, Volume 29 , ISSUE 2, 69–72

No Record Found..
Page Actions