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  • Immunohematology


Report | 09-October-2019

Trends of ABO and Rh phenotypes in transfusion-dependent patients in Pakistan

Nida Anwar, Munira Borhany, Saqib Ansari, Sana Khurram, Uzma Zaidi, Imran Naseer, Muhammad Nadeem, Tahir Shamsi

Immunohematology, Volume 32 , ISSUE 4, 170–173

Article | 27-December-2020

A simple alternative for Rh phenotyping red cells that have a persistently positive Rh control

lnvestigation of a patient's red cell sample with a persistently positive Rh control revealed that if the patient's red cells were treated with ZZAP, then incubated at 37°C with commercial Rh-typing reagents, then washed four times with saline, the positive Rh control could be circumvented and the Rh phenotype readily determined. One hundred red cell samples of known Rh phenotype were treated with ZZAP and coated with autoantibody to resemble the cells of the index case. Accurate

Jill T. Hardman, Malcolm L. Beck, Patricia Lamley

Immunohematology, Volume 5 , ISSUE 3, 83–85

Report | 01-December-2019

RHCE variant allele: RHCE*ce254G,733G

A novel RHCE allele was identified in a 53-year-old AfricanAmerican female blood donor with an Rh phenotype of D+ C– E– c+ e+ and a negative antibody screen. The donor’s cells typed e+ with all antisera tested. By gel-based genotyping and cDNA analysis, the two RHCE alleles in this donor were characterized. One allele was found to be the known allele RHCE*01.20.01 (RHCE*ce733G) and the second was novel: RHCE*01.06.02 (RHCE*ce254G,733G).

Jessica A. Keller, Trina Horn, Colleen Chiappa, Camilla Melland, Christine Vietz, Lilian Castilho, Margaret A. Keller

Immunohematology, Volume 30 , ISSUE 3, 121–122

Case report | 14-October-2020

Autoanti-D in a patient after cladribine treatment for lymphoplasmocytic lymphoma

We report the case of a 62-year-old woman who developed an autoanti-D after cladribine treatment. In May 2000, the patient underwent splenectomy for a stage IV-B lymphoplasmocytic lymphoma. She was transfused with ABO- and Rh(D)-matched blood. A month later, she received chemotherapy with cladribine. In February 2001, blood grouping showed her to be AB, D+ and the direct antiglobulin test was positive for IgG. An autoanti-D was identified in the eluate. Genotypic analysis confirmed the Rh

Joan Cid, Victor Beltran, L. Escoda, Enric Elies, Carmen Martin-Vega

Immunohematology, Volume 18 , ISSUE 1, 16–18

Case report | 09-October-2019

Autoanti-C in a patient with primary sclerosing cholangitis and autoimmune hemolytic anemia: a rare presentation

-reactive AIHA. Further testing showed the possibility of anti-C. The patient’s Rh phenotype was C+D+E–c– e+. Further testing with select cells, serial alloadsorption, and an elution confirmed anti-C specificity. The patient was transfused with two C–, crossmatch-compatible packed red blood cell units. The patient’s hemoglobin level and general condition showed improvement. This unique case report shows PSC associated with AIHA caused by autoanti-C. Usually, warm AIHA

Meenu Bajpai, Ashish Maheshwari, Shruti Gupta, Chhagan Bihari

Immunohematology, Volume 32 , ISSUE 3, 104–107

Article | 30-November-2020

Hemolytic transfusion reactions due to anti-e+f detectable only by nonstandard serologic techniques

. The patient’s RBCs typed as A, D+, probable Rh phenotype (cDE/cDE). The direct antiglobulin test was negative, and serum samples following the second transfusion were red/brown in color. Serologic investigations were inconclusive on all samples taken until February 13 (after the fourth transfusion). At this time, a weak anti-e reacting by manual polybrene technique and an anti-e+f reacting by two-stage papain technique were detected. The serum also contained potent HLA antibodies. The

Alan Devenish, Lesley A. Kay

Immunohematology, Volume 10 , ISSUE 4, 120–123

Article | 20-April-2020

Rh antigens and phenotype frequencies of the Ibibio, Efik, and Ibo ethnic nationalities in Calabar, Nigeria

nationalities were c (100%), e (96.38%), D (96.38%), E (15.22%), and C (3.62%) for the Ibibios;c (100%),e (95.60%),D (96.70%),E (21.98%),and C (0%) for the Efiks;and c (100%),e (94.29%),D (91.43%),E (28.57%), and C (2.86%) for the Ibos. The overall frequencies of the Rh antigens in these 720 individuals were c (100%), e (95.56%), D (94.44%),E (18.89%),and C (2.78%). Forty (5.56%) were found to be D–, while all were found to possess the c antigen. The most frequently occurring Rh phenotype was Dccee

Z. Awortu Jeremiah, Chris Odumody

Immunohematology, Volume 21 , ISSUE 1, 21–24

Article | 28-April-2020

The incidence of red cell alloantibodies underlying panreactive warm autoantibodies

alloantibodies not found within the Rh or Kell blood group systems. Antibodies identified included the following specificities:E (19),D (9),c (7),C (6),S (5),Fya (3),Jka (2),Jkb (2),K (2),Kpa (2),Fyb,Cw,N, and f (ce). This study reinforces the value of adsorption studies, whether using autologous or allogeneic RBCs, when panreactive warm autoantibodies are present. In addition, this study confirms that it is not appropriate in these cases simply to issue blood which is “least incompatible” or Rh

Martin Maley, David G. Bruce, Roderick G. Babb, Angus W. Wells, Mark Williams

Immunohematology, Volume 21 , ISSUE 3, 122–125

Report | 16-October-2019

Rh and Kell blood group antigen prevalence in a multi-ethnic cohort in Nigeria: implications for local transfusion service

conventional tube agglutination methods. The prevalence of the Rh antigens in the study cohort was found to be: D (92.7%), C (20.5%), c (97.7%), E (19.5%), and e (97.4%). Dce was the most common Rh phenotype (53.3%). The prevalence of K was 0 percent. For all antigens, there was no association between ethnicity and antigen prevalence. This study is the first to document the prevalence of the major Rh and K antigens in the Nigerian population, using a multi-ethnic cohort. Serologic testing demonstrates a

Ademola Samson Adewoyin, Grace Ming Lee, Titilope Adenike Adeyemo, Omolade Augustina Awodu

Immunohematology, Volume 34 , ISSUE 2, 61–65

Article | 27-April-2020

On a much higher than reported incidence of anti-c in R1R1 patients with anti-E

). Accordingly, all patients at our facility with an Rh antibody are now tested for those additional Rh antibodies they can make, as predicted from their Rh phenotype. The data from this study strongly support the selection of R1R1 RBCs for all c– patients with anti-E.

W. John Judd, Louann R. Dake, Robertson D. Davenport

Immunohematology, Volume 21 , ISSUE 3, 94–96

Report | 26-October-2019

Red cell antigen prevalence predicted by molecular testing in ethnic groups of South Texas blood donors

Scianna. Donors tested to be group O or A were serologically tested for the Rh (C, E, c, e) antigens. Those that tested as presumably R1R1, R2R2, or R0r were then genotyped. Donors constituted three major ethnicities: black (18.3%), Hispanic (36.3%), and Caucasian (41.1%); ethnicities comprised of Asian, American Indian, multiracial, and other accounted for the remaining donors (4.3%). The most likely common Rh phenotype for each ethnicity is as follows: black –R0r (44.4%), Hispanic –R1R1

Lorena I. Aranda, Linda A. Smith, Scott Jones, Rachel Beddard

Immunohematology, Volume 31 , ISSUE 4, 166–173

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