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  • Immunohematology


Article | 01-April-2020

H-deficient Bombay and paraBombay red blood cells are most strongly agglutinated by the galactophilic lectins of Aplysia and Pseudomonas aeruginosa that detect I and P1 antigens

europaeus (UEA-I) and Pseudomonas aeruginosa lectin (PAIIL), as well as with those achieved with anti-I serum. The results revealed that, in contrast to UEA-I and PA-IIL, which preferentially agglutinated H+ RBCs, and to MPL and PNA, which similarly agglutinated all examined RBCs, AGL, PA-IL, and the anti-I serum agglutinated the H-deficient RBCs more strongly than did the H+ RBCs. These findings could be attributed to increased levels of I and P1 antigens on those RBCs resulting from the use of the

Nechama Gilboa-Garber, Dvora Sudakevitz, Cyril Levene, Naomi Rahimi-Levene, Vered Yahalom

Immunohematology, Volume 22 , ISSUE 1, 15–22

Report | 29-October-2019

I-int phenotype among three individuals of a Parsi community from Mumbai, India

present study was undertaken. The RBCs of randomly selected subjects were screened with anti-I and anti-i reagents by a saline tube technique at 22°C. Individuals with unusual I and i antigen reactivity patterns were further tested by a semi-quantitative method with a battery of anti-I and anti-i reagents, followed by family studies. Three of the 5864 donors tested showed an elevated strength of i antigen. Further study revealed an intermediate strength of both I and i antigens compared with those

Sanmukh R. Joshi

Immunohematology, Volume 30 , ISSUE 1, 11–13

Article | 31-December-2020

Cold Autoimmune Hemolytic Anemia With Auto-Anti-AI Specificity: 51Chromium Studies

A 65-year-old woman was found to have severe autoimmune hemolytic anemia. The patient was group A1, RhoD positive. The direct antiglobulin test was strongly positive with anti-C3 and negative with anti-IgG. The serum contained two distinct IgM antibodies, auto-anti-I and auto-anti-AI. Both were reactive at 22°C. However, the anti-AI also was reactive in saline and in albumin at 37°C. An eluate revealed anti-AI and a weak anti-I. Sequential 51Chromium survival studies were done with

May H. McGinniss, Richard J. Davey, Richard A. Binder, Arthur N. Kales

Immunohematology, Volume 3 , ISSUE 2, 20–22

Article | 03-November-2020

Comparison of gel technology and red cell affinity column technology in antibody detection

anti-D due to Rh immune globulin but missed one anti-Jka. ReACT missed one anti-D and one anti-Jkb. MTS-Gel detected one anti-I and one anti-H whereas ReACT detected two antiH but not anti-I. No false positive reactions were found by either method. Sensitivity based on this study for MTS-Gel is 93.3% and ReACT is 86.7%. Advantages for MTS-Gel included the small volume needed for testing, and the reaction was stable for 48 hours; for ReACT, there was less spin time and no special pipette was needed

Sauvai I. Chanfong, Sherri Hill

Immunohematology, Volume 14 , ISSUE 4, 152–154

Article | 26-October-2020

A comparison of a new affinity column system with a conventional tube LISS-antiglobulin test for antibody detection

important antibodies, including 36 antibodies that reacted ≤ 1+ at LISS-AGT (0% falsely negative). Eleven of 16 (69%) clinically benign antibodies reacted by AFC. Five samples (2 anti-Sda, 2 anti-I, and 1 inconclusive) were negative by AFC. AFC tests were negative with all 130 samples that were negative by LISS-AGT (0% falsely positive). The AFC method showed results comparable with results obtained with a conventional tube LISS-AGT for detection of clinically important antibodies. Some unwanted

R. Sue Shirey, Joan S. Boyd, Christine Barrasso, Karen E. King, Paul M. Ness

Immunohematology, Volume 15 , ISSUE 2, 75–77

Case report | 21-March-2020

Hyperhemolytic transfusion reaction attributable to anti-Fy3 in a patient with sickle cell disease

possible anti-I specificity. A DAT performed on her RBCs was negative.RBC units that lacked E,C,Jkb,N,V,and S were transfused. Posttransfusion Hb was 8.9 g/dL. On day 10 she developed a fever of 103°F,and on day 11 her Hb decreased to 6.4 g/dL. She complained of severe back pain and dark urine. In addition,she became hypertensive,tachycardic,and jaundiced. The DAT indicated the presence of IgG on the patient’s RBCs. Anti-Fy3 was identified in the serum and eluate. During the next 24 hours,her

Meredith A. Reyes, Orieji C. Illoh

Immunohematology, Volume 24 , ISSUE 2, 45–51

Article | 03-November-2020

Improved detection of weak, clinically significant antibodies by supplementation of polyethylene glycol with a low-ionic solution

. With the exception of anti-P1, anti-I, and an anti-Lea, PEG-IAGTs detected all the antibodies tested compared with 72.3% and 77.4% for saline-IAGTs and RAM-IAGTs, respectively. The end-point titers of at least 82% of antibodies detected by PEG-IAGTs were 1–3 dilutions higher than those by saline- and RAM-IAGTs. When specificity of PEG-IAGTs was tested using 268 randomly selected, fresh (< 1 day old) blood samples, PEG-IAGT detected 11 out of 268 samples as positive compared with 7 out of

Kim Swee Low, Yew-Wah Liew, Peter M. Bradley

Immunohematology, Volume 14 , ISSUE 2, 68–71

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