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  • Immunohematology


Report | 12-March-2020

Determination of optimal method for antibody identification in a reference laboratory

Methods commonly used for antibody identification are hemagglutination (tube), column agglutination (gel), and solid-phase red cell adherence. Our AABB immunohematology reference laboratory (IRL) conducted a study to determine which antibody identification testing method was optimal for detecting all clinically significant antibodies. Patient specimens were sent to our IRL from August 2008 to September 2009. Routine testing was performed by tube method and then by manual gel and manual solid

Jennifer R. Haywood, Marilyn K. Grandstaff Moulds, Barbara J. Bryant

Immunohematology, Volume 27 , ISSUE 4, 146–150

Article | 14-October-2020

Enzyme and DTT treatment of adherent RBCs for antibody identification by a solid phase immunoassay system

Treatment of RBCs with protease enzymes or dithiothreitol (DTT) causes denaturation of several RBC antigens and is regularly used in antibody identification. In this study, we have standardized enzyme and DTT treatment of adherent RBCs in the magnetic-mixed passive hemagglutination assay (M-MPHA) for antibody identification. We have also tried drying these treated RBCs. The optimal enzyme and DTT treatment conditions for intact adherent RBCs were determined, in addition to the optimal condition

Toyohiro Tamai, Toshio Mazda

Immunohematology, Volume 18 , ISSUE 4, 114–119

Article | 17-November-2020

Antibody identification reports: a microcomputer program

Antibody identification involves preparation of a comprehensive report for the benefit of the referring physician. These reports require technical, supervisory, and clerical time to produce a quality report. To standardize laboratory reporting of immunohematologic test results in an increasingly cost-conscious environment, we developed computer-generated laboratory report forms. These forms permit the reference laboratory technician to produce final reports with only a few keystrokes. Use of

Mercy Kuriyan, Laurence Marsh

Immunohematology, Volume 11 , ISSUE 2, 54–59

Article | 14-December-2020

Procedural errors in antibody identification

In experimental studies of students and line technologists perfoming antibody identification procedures, both groups made errors. These errors included, at times, either failing to identify an antibody or misidentifying the specficity(ies). A prospective study was undertaken to identify errors made in a laboratory setting. Errors were classified as 1) failing to follow protocol (procedural error) or 2) arriving at the wrong answer (misidentification error). Over a 1-year period, 1,057 workups

Patricia L. Strohm, Philip J. Smith, Jane M. Fraser, Thomas E. Miller, Sally V. Rudmann, Jack W. Smith, Jr., John R. Svirbely, Janice F. Blazina, Melanie S. Kennedy

Immunohematology, Volume 7 , ISSUE 1, 20–22

Article | 15-February-2021

Albumin-indirect antiglobulin test

. Reagents/Supplies Reagents Supplies Reagent RBCs for antibody detection and/or antibody identification 22% bovine albumin 0.9% saline or PBS pH 6.5–7.5 AHG (polyspecific or anti-IgG) IgG-coated reagent RBCs Test tubes (10 × 75 or 12 × 75 mm) Pipettes Calibrated serofuge and/or cell washer Calibrated timer RBCs = red blood cells; PBS = phosphate-buffered saline; AHG = antihuman globulin. Procedural Steps Add two drops plasma or serum to a labeled tube. Add one drop

J.R. Hamilton

Immunohematology, Volume 35 , ISSUE 2, 63–64

Article | 16-February-2021

Saline–indirect antiglobulin test

the IAT, can also be informative in antibody identification studies. A saline-IAT can be made more sensitive by adding more plasma/serum to increase the serum:cell ratio, since the increased plasma/serum provides more antibody for binding to the antigen sites. This modification for increased sensitivity cannot be used in tests potentiated by commercial preparations of low-ionic-strength solution (LISS) or polyethylene glycol (PEG), which is often prepared as a low-ionic-strength reagent. Any

J.R. Hamilton

Immunohematology, Volume 35 , ISSUE 4, 156–158

Article | 16-October-2019

Separation of multiple antibodies by adsorption with allogeneic red blood cells

Principle Antibody detection and identification are processes that are commonly performed in the transfusion service before the transfusion of allogeneic red blood cells (RBCs). Antibody identification usually follows the discovery of a positive antibody detection test, or other factors such as ABO serum/cell discrepancy or incompatible crossmatch.1 Antibody identification is a necessary practice in blood banking to determine blood products that are suitable for transfusion to an individual

E.M. Ekema

Immunohematology, Volume 33 , ISSUE 4, 155–158

Article | 18-October-2020

Comparison of tube and gel techniques for antibody identification

There are several methods for antibody detection and each technique has advantages and limitations. We compared the performance of the tube (polyethylene glycol–indirect antiglobulin test [PEG-IAT]) and gel test technique for antibody identification. From January to May 1999, we performed antibody screening tests by gel and tube techniques on 10,123 random blood samples submitted to our reference laboratory. Six hundred and twentyeight (6.2%) reactive samples were tested for antibody

Marcia Cristina Zago Novaretti, Eduardo Jens Silveira, Edio da Costa Filho, Pedro Enrique Dorlhiac- Llacer, Dalton de Alencar Fischer Chamone

Immunohematology, Volume 16 , ISSUE 4, 138–141

Article | 03-November-2020

The gel test: use in the identification of unexpected antibodies to blood group antigens

The IgG GEL test was compared with the LISS tube test (Löw and Messeter’s low-ionic-strength saline) for antibody identification. The suitability of red blood cells (RBCs) pretreated with ficin, dithiothreitol (DTT), or chloroquine diphosphate (CDP) also was assessed for use in the GEL test. In addition, time-in-motion studies were performed comparing GEL (12 panels per batch) with polyethylene glycol (PEG) tube tests (3 panels per batch). In 57 antibody identification studies, there

W. John Judd, E.Ann Steiner, Pamela C. Knaf, Colleen Masters

Immunohematology, Volume 14 , ISSUE 2, 59–62

Case report | 01-December-2019

Performance of an automated solid-phase  red cell adherence system compared with  that of a manual gel microcolumn assay for  the identification of antibodies eluted from  red blood cells

IgG antibodies coating red blood cells (RBCs) can be removed by elution procedures and their specificity determined by antibody identification studies. Although such testing is traditionally performed using the tube agglutination assay, prior studies have shown that the gel microcolumn (GMC) assay may also be used with comparable results. The purpose of this study was to compare an automated solid-phase red cell adherence (SPRCA) system with a GMC assay for the detection of antibodies eluted

Rachel H. Finck, Rebecca J. Davis, Shih-Mao Teng, Dennis Goldfinger, Alyssa F. Ziman, Qun Lu, Shan Yuan

Immunohematology, Volume 27 , ISSUE 1, 1–5

Review | 09-October-2019

How to recognize and resolve reagentdependent reactivity: a review

patient transfusion. It is imperative that reagent-dependent reactivity is recognized and resolved during the investigation of ABO discrepancies, positive RBC antibody screens and antibody identification panels, and crossmatch reactivity.

Gavin C. Patch, Charles F. Hutchinson, Nancy A. Lang, Ghada Khalife

Immunohematology, Volume 32 , ISSUE 3, 96–99

Article | 06-December-2020

Identifying blood group antibodies...can a computer help?

Antibody identification is one of the last areas of laboratory medicine to embrace computerization. This is due in part to the complex nature of antibody identification. Until recently, computer programs written to assist with antibody identification have been slow and cumbersome. However, technological advances in computer hardware have greatly improved the response time for these applications. Still, blood bank technologists sometimes shun assistance from the computer because they consider

Glen Dietz, Nancy J. Miller

Immunohematology, Volume 9 , ISSUE 2, 53–55

Article | 20-December-2020

Red cell antibody identification by solid phase red cell adherence utilizing dried RBC monolayers

Darryl L. Stone, Ralph A. Eatz, Susan D. Rolih, Seaborn J. Farlow, Gordon S. Hudson, Lyle T. Sinor

Immunohematology, Volume 6 , ISSUE 1, 12–17

Article | 14-October-2020

Evaluation of a new solid-phase immunoassay for alloantibody detection using bromelin-treated and untreated red blood cells

The enzyme test is used to detect certain antibodies or facilitate antibody identification. This study compares antibody reactivity with bromelin-treated red blood cells (RBCs) and untreated RBCs using a newly developed solid-phase immunoassay. The reactivity of irregular antibodies was tested by a magnetic-mixed passive hemagglutination assay (M-MPHA). In addition, antibody reactivity was tested with dried stroma of bromelin-treated RBCs and untreated RBCs (M-MPHA-Dry). Rh antibodies were

Toyohiro Tamai, Toshio Mazda

Immunohematology, Volume 17 , ISSUE 1, 17–21

Review | 01-December-2019

Warm autoadsorption with enzyme-treated red blood cells

Patients demonstrating warm autoantibody specificity present serologic challenges for laboratory staff performing antibody identification in the blood bank. Autoantibody can be removed from plasma or serum by adsorption onto autologous red blood cells (RBCs) provided the patient has not been transfused in the previous 3 months. The adsorption process can be enhanced by enzyme pretreatment of autologous RBCs.

Farai Tsimba-Chitsva, Susanne Bishop, Kelly Kezeor

Immunohematology, Volume 28 , ISSUE 3, 88–90

Article | 03-November-2020

Comparison of affinity column technology and LISS tube tests

Kayla D. Champagne, Peggy Spruell, Jane Chen, Leslie Voll, Gloria Schlanser, Marilyn Moulds

Immunohematology, Volume 14 , ISSUE 4, 149–151

Article | 01-April-2020

External quality assessment scheme in red blood cell serology: a 5-year experience in Thailand

From 2000 to 2004, 36, 58, 72, 78,and 86 laboratories participated in an external quality assessment scheme (EQAS) organized by the Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital. Each year the staff was requested to perform ABO grouping,D typing,antibody screening,antibody identification,and DATs on eight blood samples. Each participant received information on the correct test results and a coded summary. Regarding ABO grouping, the error .rate ranged from 0.3 to 1.3

Sasitorn Bejrachandra, Jariya Saipin, Oytip Nathalang, Usanee Siriboonrit, Ekaraj Rungroung, Sudjai Udee

Immunohematology, Volume 22 , ISSUE 1, 1–5

Article | 14-December-2020

Primary immune response to blood group antigens in burned children

positive direct antiglobulin test (DAT), or both. None of the 11 patients included in the study had been previously tranfused or pregnant. The average number of units transfused prior to antibody identification was 19. The average time elapsed between the first transfusion and antibody identification was 3.6 weeks. Anti-K and anti-E were the most frequently identified. Three patients had a decrease in hemoglobin (average 1.5 g/dL) and hematocrit at the time that a positive DAT was detected. Such

Nancy E. Bacon, Ethel D. Patten, Janet L. Vincent

Immunohematology, Volume 7 , ISSUE 1, 8–11

Article | 14-October-2020

Confirmation that the JAHK antigen is associated with the rG haplotype

Anti-JAHK, an antibody directed toward a low-incidence antigen in the Rh system, was detected during routine antibody identification in a male donor who had no history of transfusion. Examples of anti-JAHK have been found in sera containing multiple antibodies to low-incidence antigens. The first report of anti-JAHK was in 1995 and described the association of the JAHK antigen with the rG haplotype. Our results confirm this association.

Joanne Kosanke, Jill Storry, Marion Reid

Immunohematology, Volume 18 , ISSUE 2, 46–47

Article | 16-October-2019

Cold autoadsorption

Cold-reactive autoantibodies can mask the presence of underlying clinically significant alloantibodies in a patient’s plasma or serum. These autoantibodies are problematic when performing laboratory procedures such as ABO typing, red blood cell (RBC) crossmatching, antibody detection testing, and antibody identification. To avert the masking of clinically significant alloantibodies in a patient’s plasma or serum, adsorption studies can be performed at 4°C using autologous RBCs

Ernest M. Ekema

Immunohematology, Volume 34 , ISSUE 4, 158–160

Article | 29-December-2020

Use of a personal computer to determine the statistical validity of antibody identification by Fisher's exact method

Robert J. Eckrich

Immunohematology, Volume 4 , ISSUE 2, 34–37

Article | 14-October-2020

Equivalence of spray-dried K2EDTA,spray-dried K3EDTA, and liquid K3EDTA anticoagulated blood samples for routine blood center or transfusion service testing

We compared the results of routine blood tests for 102 blood donors’samples and 100 patients’samples collected in spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes to evaluate the impact of changes in formulation of the anticoagulant (K2EDTA vs.K3EDTA), its application (liquid vs.spraydried), and tube material (glass vs. plastic). Methods for ABO/D testing, antibody screening, and antibody identification included direct hemagglutination/microplate

Stacie Leathem, Nicole Dodge Zantek, Marti Kemper, Laura Korte, Al Langeberg, S. Gerald Sandler

Immunohematology, Volume 19 , ISSUE 4, 117–121

Article | 30-November-2020

A survey of management of blood donations with unexpectected red cell antibodies

A survey of 19 large blood centers, each with an average annual collection of 158,889 units, was conducted to identify current practices of management of red cell units that contain unexpected red cell antibodies. The routine antibody identification, distribution, and charge for such units varied widely among the 19 centers. In addition, although use of these units was low, costs were considerable. This survey can serve as a basis for other studies to arrive at cost-effective ways to manage

Marlene Simpson, Jana Julleis, Ram Kakaiya

Immunohematology, Volume 10 , ISSUE 3, 99–101

Article | 03-November-2020

Comparison of gel technology and red cell affinity column technology in antibody detection

Sauvai I. Chanfong, Sherri Hill

Immunohematology, Volume 14 , ISSUE 4, 152–154

Report | 01-December-2019

Seroprevalence of unexpected red blood cell antibodies among pregnant women in Uganda

identification. Of the 1001 blood samples tested, 48.9 percent, 26.4 percent, 21.0 percent, and 3.8 percent tested positive for blood groups O, A, B, and AB, respectively. Of these samples, 23 (2.3%) were negative for D, and 55 (5.5%) showed initial reactivity with at least one screening RBC. The RBC antibody screen was repeated on these 55 samples, and antibody identification was performed at the Johns Hopkins Hospital Blood Bank in Baltimore, Maryland. Twenty-one of the 55 samples were confirmed to have

Kristina Eipl, Clemensia Nakabiito, Kabali Bwogi, Mahnaz Motevalli, Angela Roots, Lorraine Blagg, J. Brooks Jackson

Immunohematology, Volume 28 , ISSUE 4, 115–117

Article | 09-November-2020

Anti-Jk3 with no clinical evidence of HDN

A sample was submitted to a reference lab from a 27-year-old Asian female, gravida 4 para 1, for antibody identification. Anti-Jk3 with an IgM component was identified. Subsequently, the antibody was eluted from the infant’s cord and venous red blood cells. Normal bilirubin and hematocrit levels ruled out hemolytic disease of the newborn (HDN). Anti-Jk3 has been implicated in two cases of mild HDN. In this case, this noncomplement-binding antibody caused a positive direct Coombs test

Celeste J. Hunter, Michael D. Ziebol

Immunohematology, Volume 13 , ISSUE 4, 136–137

Article | 22-January-2021

Freezing and recovering rare red blood cells using glycerol

identified in donors or patients, a blood bank or an IRL can freeze and store them, thawing them when needed for antibody identification or compatibility testing. RBCs can be frozen in aliquots so only the portion required for immediate testing need be thawed, thus preventing exposure to excessive freeze/thaw cycles. Procedures for freezing and thawing RBCs should provide the highest RBC recovered percentage to ensure a usable sample. Anyone who has performed a Lui freeze-thaw elution knows that putting

B. Eades

Immunohematology, Volume 36 , ISSUE 3, 85–88

Article | 06-December-2020

Effect of enzymes on and chemical modifications of high-frequency red cell antigens

Enzyme or chemical modification of intact red cells results in the destruction of some blood group antigens. The pattern of reactions of an antibody with red cells treated with various proteinases, with sialidase, and with the disulfide bond-reducing agent 2-aminoethylisothiouronium bromide (AET) can aid in antibody identification. This information can prove particularly beneficial with antibodies to antigens of very high frequency, where antigen-negative cells may be difficult to obtain

Geoff Daniels

Immunohematology, Volume 8 , ISSUE 3, 53–57

Article | 17-February-2021

Elimination of HLA antibodies by platelet adsorption

J. Jung, C. Barron

Immunohematology, Volume 36 , ISSUE 1, 1–3

Article | 03-November-2020

Implementation of gel column technology, including comparative testing of Ortho ID-MTS with standard polyethylene glycol tube tests

antibody enhancement. Tests were performed as described in the manufacturer’s guidelines and the current edition of the Technical Manual of the American Association of Blood Banks. Testing included antibody detection, antibody identification, direct antiglobulin tests (DATs), antigen phenotyping (K, Fya, Fyb, S, and s), and elution studies. These procedures were evaluated for sensitivity, specificity, and efficiency. Sixty-six samples that had been tested for antibody activity by PEG tube

Diane A. Derr, Stacy J. Dickerson, E.Ann Steiner

Immunohematology, Volume 14 , ISSUE 2, 72–74

Case report | 26-October-2020

Case report: passively acquired anti-D in a D+ pregnant patient

A sample was submitted for serologic evaluation from a pregnant patient with immune thrombocytopenic purpura (ITP) for possible transfusion in the future because of a decreased platelet count. Anti-D and -E were identified in the patient’s serum using several antibody identification techniques, and anti-D was recovered in an acid eluate prepared from the patient’s red cells. It was discovered that WinRho™ had been administered to treat the ITP. This product has been licensed

Marie P. Holub, Kirk D. Kitchen, Eugene Mensinger

Immunohematology, Volume 15 , ISSUE 2, 69–70

Article | 15-April-2020

In search of the Holy Grail: comparison of antibody screening methods

Tony S. Casina

Immunohematology, Volume 22 , ISSUE 4, 196–202

Article | 16-October-2019

Low-ionic-strength saline solution–antiglobulin test (LISS-AGT)

suspended in LISS. Modifications of the method led to development of the commercially prepared LISS additive solutions in use today. The LISS-AGT can be used effectively to detect alloantibodies of all major blood groups in antibody detection, antibody identification, and crossmatching procedures.

LeeAnn Walker

Immunohematology, Volume 34 , ISSUE 2, 57–60

Article | 10-April-2021

A fatal case of acute hemolytic transfusion reaction caused by anti-Wra: case report and review of the literature

. Immunohematologic results Test parameter Patient RBC unit 1 RBC unit 2 ABO, D A1, D– O, D– A, D– Wra phenotype Wr(a–) Wr(a–) Wr(a+b+) Anti-Wra titer IgM 32, IgG 1024 NA NA Antibody detection test Negative* Negative† NA NA Initial antibody identification Negative* Negative† NA NA DAT Negative* Negative† NA NA IS crossmatch NA Negative Positive (4+)* Negative† IAT crossmatch NA Negative Positive (4+)* Positive (3+)† *Results pre-transfusion reaction. †Results post-transfusion

A. Espinosa, L.J. Garvik, N. Trung Nguyen, B. Jacobsen

Immunohematology, Volume 37 , ISSUE 1, 20–24

Report | 16-October-2019

Method-specific and unexplained reactivity in automated solid-phase testing and their association with specific antibodies

antibodies (Abs). In this study, nonspecific reactivity (NS) is defined as methodspecific panreactivity detected by solid-phase testing only, with no reactivity in other methods. Unexplained reactivity (UR) is defined as reactivity present and detectable in all test methods after all clinically significant antibodies were ruled out following a standard antibody identification algorithm using selected cell panels. This retrospective study evaluated antibody detection tests of patients at a single center

Mary E. Harach, Joy M. Gould, Rosemary P. Brown, Tricia Sander, Jay H. Herman

Immunohematology, Volume 34 , ISSUE 3, 93–97

Article | 13-April-2020

Problems highlighted when using anticoagulated samples in the standard tube low ionic strength antiglobulin test

Within the UK blood transfusion services, there is currently no recommendation for the use of either clotted or anticoagulated samples for antibody identification testing. This report describes three cases in which the detection of IgM antibodies was impeded by the use of anticoagulated samples. Two patient samples,referred for compatibility testing, were both identified as having IgM complement-activating anti-S and the remaining case involved an antenatal patient with IgM complement

Amanda J Sweeney

Immunohematology, Volume 22 , ISSUE 2, 72–77

Article | 16-November-2020

Empirical evaluation of the transfusion medicine tutor

Previous research during the development of Antibody IDentification Assistant (AIDA) revealed that many medical technology students and other laboratory personnel have serious difficulties in determining the specificity of blood group alloantibodies, especially weak or multiple antibodies. Based on these previous results, AIDA was modified to provide a teaching environment for medical technology students. We report the results of a rigorous, objective evaluation of the resultant system, the

Jodi Heintz Obradovich, Philip J. Smith, Stephanie Guerlain, Sally Rudmann, Patricia Strohm, Jack Smith, John Svirbely, Larry Sacks

Immunohematology, Volume 12 , ISSUE 4, 169–174

Article | 22-January-2021

Anti-Ata in a renal transplant candidate: a case report

/L) and decreased total bilirubin of 0.2 mg/mL (normal 0.3–1.9 mg/dL). These abnormal chemistry results were attributed to renal failure. In her pretransplant evaluation, testing for antibodies to class I and class II human leukocyte antigens (HLAs) was performed. The results showed large amounts of HLA antibodies present, with levels of 99 and 98 percent panel reactive antibodies for class I and class II, respectively. Antibody Detection Testing and Antibody Identification Blood type and

J. Gao, S. Wise, S.H. Tinsley, J.F. Shikle

Immunohematology, Volume 36 , ISSUE 3, 104–107

Article | 30-November-2019

Inhibition of blood group antibodies by soluble substances

identification of antibodies to high-prevelance antigens that are single pass and glycosylphosphatidylinositol-linked proteins, thus leading to the detection of underlying alloantibodies.5–8 Unlike traditional soluble substances that are found naturally in human and other animal sources, rBGPs are manufactured.5–7 The result of the manufacturing is a very specific rBGP that could aid in antibody identification.7 Gone are the days when the only tools available to the investigational immunohematologist were

K.M. Byrne, C.M.C. Mercado, T.N. Nnabue, T.D. Paige, W.A. Flegel

Immunohematology, Volume 35 , ISSUE 1, 19–22

Report | 01-December-2019

Single-center comparison of gel microcolumn and solid-phase methods for antibody screening

Our facility changed antibody screening methods from a gel microcolumn–based test (ID-Micro Typing System Gel Test; Ortho Clinical Diagnostics, Inc., Raritan, NJ) to an automated solid-phase test (Galileo/Capture-R Ready-Screen [I and II], Immucor, Inc., Norcross, GA). To determine whether detection rates for commonly encountered clinically significant red blood cell antibodies differed as a consequence of this change, preimplementation and postimplementation antibody identification

Anne Schmidt, Brenda J. Bendix, Eapen K. Jacob, Sandra C. Bryant, James R. Stubbs

Immunohematology, Volume 29 , ISSUE 3, 101–104

Report | 12-March-2020

RHCE*ceAR encodes a partial c (RH4) antigen

procedures. RBCs from the patient typed C+c+ but his plasma contained alloanti-c. DNA analyses showed the presence of RHCE*Ce in trans to RHCE*ceAR with RHD*D and RHD*Weak D Type 4.2.2. The amino acid changes on RhceAR are such that a C+c+ patient made alloanti-c. This case shows that RhceAR carries a partial c antigen and illustrates the value of DNA testing as an adjunct to hemagglutination to aid in antibody identification in unusual cases.

Marion E. Reid, Christine Halter Hipsky, Christine Lomas-Francis, Akiko Fuchisawa

Immunohematology, Volume 26 , ISSUE 2, 57–59

Case report | 01-December-2019

A case of masquerading alloantibodies:  the value of a multitechnique approach

In an immunohematology reference laboratory, samples received for antibody identification react in many different ways requiring a variety of approaches. Sometimes, the clues from initial testing can lead to faulty assumptions and misdirection. Fortunately, a well-supplied reference laboratory will have access to a variety of techniques and reagents that, when used together, can reveal the true identity of the antibodies involved. We present a case of a patient sample with an apparent group AB

Paula M.S. Wennersten, Laurie J. Sutor

Immunohematology, Volume 30 , ISSUE 3, 117–120

Article | 10-April-2021

Acute hemolytic transfusion reaction caused by anti-Yta

nurses collected and arranged the transport of samples to the hospital. From the hematologic status at the nursing facility, the patient’s Hb was 12.7 g/dL. A few weeks later, samples that had been collected while the individual was in outpatient care were received for antibody identification and sent to the International Reference Laboratory (IRL). In the plasma of the patient, the following complex mixture of antibodies was revealed: anti-Yta, -D, -C, -Leab, and -HI. A variety of methods, including

M. Raos, N. Thornton, M. Lukic, B. Golubic Cepulic

Immunohematology, Volume 37 , ISSUE 1, 13–17

Article | 30-November-2020

Misidentification of anti-Vel due to inappropriate use of prewarming and adsorption techniques

-RBCs. Adsorptions with rabbit RBCs did not affect reactivity. Pretransfusion RBCs were nonreactive with three examples of anti-Vel at the IAT. The posttransfusion serum was grossly hemolyzed and anti-Vel was demonstrable, although weaker than in the pretransfusion sample. The antibody was subclassed as IgG1 and IgG3. In this case, the use of a prewarming technique precluded detection of hemolysis caused by the antibody prior to the IAT. This case stresses the importance of antibody identification

Jill Storry, Delores Mallory

Immunohematology, Volume 10 , ISSUE 3, 83–86

Case report | 26-October-2019

Anti-Jk3 in a Filipino man

ordered for transfusion. During subsequent pre-transfusion compatibility testing, the antibody screen was found to be positive (all screening cells reactive at the antihuman globulin phase). An antibody identification panel was performed. The patient’s serum was found to react with all panel cells tested, including the autocontrol tube. A direct antiglobulin test revealed the presence of both anti-IgG and anti-C3 coating the patient’s RBCs. The specimen was then sent to a reference

Shaina McCaskill, Scott Wise, Sheila Tinsley

Immunohematology, Volume 31 , ISSUE 3, 119–122

Article | 27-April-2020

On a much higher than reported incidence of anti-c in R1R1 patients with anti-E

A previous study involving tube IATs, untreated RBCs, and a lowionic-strength additive reagent revealed that approximately onethird of R1R1 patients with anti-E have a concomitant anti-c. However,the current study finds a much higher incidence of anti-c in such patients, using gel technology in conjunction with ficinpretreated RBCs. Results of antibody identification studies and transfusion records of 82 R1R1 patients with anti-E were reviewed. Serologic test methods included a LISS wash

W. John Judd, Louann R. Dake, Robertson D. Davenport

Immunohematology, Volume 21 , ISSUE 3, 94–96

Case report | 09-October-2019

A suspected delayed hemolytic transfusion reaction mediated by anti-Joa

A 32-year-old African-American woman with a history of sickle cell disease presented for surgical evaluation of left total hip arthroplasty due to avascular necrosis of the femoral head. In anticipation of a complex orthopedic procedure, pre-surgical blood work was ordered. The patient’s Fenwal blood sample typed as group O, D+. Although the patient had a history of anti-Fya, the antibody identification was inconclusive, so the workup was sent to a reference laboratory. The patient was

Ryan P. Jajosky, Wendy C. Lumm, Scott C. Wise, Roni J. Bollag, James F. Shikle

Immunohematology, Volume 33 , ISSUE 2, 73–75

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