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  • Immunohematology


Article | 14-December-2020

Decreased ABH blood group antigen expression associated with preleukemic conditions and acute leukemia: loss of detectable B, then A antigens in a group AB patient progressing from a myelodysplastic syndrome to leukemia

Decreased or absent expression of blood group antigens is well known to occur in acute leukemia. In some carcinomas, the malignant solid tumor cells have also been shown to lose normal blood group antigen expression. In both carcinoma and hematologic malignancies, these findings have been associated with a more aggressive behavior of the neoplasm. A 34-year-old, group AB, Rh positive woman was diagnosed with a preleukemic condition, myelodysplastic syndrome, in December 1988. In April 1989 B

Kaaron Benson

Immunohematology, Volume 7 , ISSUE 4, 89–93

Report | 17-March-2020

Southeast Asian ovalocytosis is associated with increased expression of Duffy antigen receptor for chemokines (DARC)

Ian J. Woolley, Paul Hutchinson, John C. Reeder, James W. Kazura, Alfred Cortés

Immunohematology, Volume 25 , ISSUE 2, 63–66

Report | 25-March-2020

Rapid, single-subject genotyping to predict red blood cell antigen expression

Stefanie L. Slezak, Sharon Adams, Hallie Lee-Stroka, Joshua E. Martin, Lorraine Caruccio, David F. Stroncek

Immunohematology, Volume 24 , ISSUE 4, 154–159

Article | 30-November-2020

Loss and reappearance of RhO(D) antigen on the red blood cells of an individual with acute myelogenous leukemia

Complete loss of Rho(D) antigen from red blood cells (RBCs) of individuals with hematologic disorders, though not frequent, has been reported. This case reports the loss of D antigen on the RBCs of a patient with acute myelogenous leukemia and its reappearance when he was in remission. Loss of D antigen expression coincided with worsening clinical and cytogenetic disease. At the time of D antigen loss, the patient also had cytogenetic abnormalities in the bone marrow cells. When he was in

Kala Mohandas, Vesna Najfeld, Harriet Gilbert, Penny Azar, Donna Skerrett

Immunohematology, Volume 10 , ISSUE 4, 134–135

Case report | 01-December-2019

Molecular RH blood group typing of serologically D–/CE+ donors: the use of a polymerase chain reaction–sequence-specific primer test kit with pooled samples

Donatella Londero, Mauro Fiorino, Valeria Miotti, Vincenzo de Angelis

Immunohematology, Volume 27 , ISSUE 1, 25–28

Report | 09-October-2019

A detailed flow cytometric method for detection of low-level in vivo red blood  cell–bound IgG, IgA, and IgM

Flow cytometric methods are commonly used to analyze white blood cell surface antigen expression. We developed a flow cytometric method to detect red blood cell (RBC)-bound immunoglobulin (Ig)G, IgA, and IgM. RBCs were washed; incubated with fluorescein isothiocyanate (FITC)-conjugated anti-IgG, -IgA, or -IgM; washed; and analyzed on the flow cytometer. The method was optimized by determining the dilution of FITC-conjugated anti-IgG, -IgA, and -IgM providing the greatest amount of fluorescence

Wendy Beres, Geralyn M. Meny, Sandra Nance

Immunohematology, Volume 32 , ISSUE 4, 161–169

Article | 22-January-2021

The P1PK blood group system: revisited and resolved

L. Stenfelt, Å. Hellberg, J.S. Westman, M.L. Olsson

Immunohematology, Volume 36 , ISSUE 3, 99–103

Article | 26-October-2019

Mass-scale donor red cell genotyping using real-time array technology

Blood centers are in the unique position to evaluate large numbers of blood donations for antigen-negative blood types. The limitations with the use of hemagglutination, however, can be circumvented with red cell genotyping. The reagents used for genotyping are synthesized and can be designed for any of the known blood group antigen single nucleotide polymorphisms that are associated with blood group antigen expression. There is interest in the application of mass-scale red cell genotyping of

Gregory A. Denomme, Michael J. Schanen

Immunohematology, Volume 31 , ISSUE 2, 69–74

Article | 22-November-2020

Use of monoclonal Jka and Jkb reagents in phenotyping red cells with a positive direct antiglobulin test

an indirect antiglobulin test. The Jka and Jkb MAbs consistently gave the same phenotype results both on untreated DAT-positive red cells and on the same cells after CDP treatment. Two of the CDPtreated samples had diminished antigen expression with the MAbs, a finding that may have been caused by the CDP treatment. One untreated sample, which spontaneously agglutinated in a lowprotein medium, was incorrectly phenotyped with the anti-Jka MAb, but both MAbs and PAbs gave the same correct results

Judy L. Brazell

Immunohematology, Volume 10 , ISSUE 1, 16–18

Article | 10-November-2020

Do monocyte ADCC assays accurately predict the severity of hemolytic disease of the newborn caused by antibodies to high-frequency antigens?

related to fetal antigen expression and tissue distribution. The anti-Inb was further investigated using mono­cyte binding and inhibition studies. The absence of HDN in the pres­ence of potent anti-U remains unexplained. In general, antibodies with low ADCC activity do not cause HDN, but high ADCC activity does not always indicate severe HDN.

Stephen F. Garner, Alan Devenish

Immunohematology, Volume 12 , ISSUE 1, 20–26

Report | 29-October-2019

I-int phenotype among three individuals of a Parsi community from Mumbai, India

The red blood cells (RBCs) of most adult individuals display an I+i– phenotype, whereas those of newborns and some rare adult individuals are typed as I–i+. The phenotype in the latter category, designated as adult i, is under genetic influence as the RBCs of I+i+ individuals  display strengths of I and i antigen expression intermediate to that of ordinary adults and ii-adults. As there was no information on the occurrence of adult i phenotype in the Indian population, the

Sanmukh R. Joshi

Immunohematology, Volume 30 , ISSUE 1, 11–13

Article | 16-November-2020

A method to detect McLeod phenotype red blood cells

flow cytometry (control cells 441). Monoclonal anti-k (F7) and human polyclonal anti-k (C30A-1) gave stronger reactions by hemagglutination with RBCs from McLeod males and were not appropriate to differentiate RBCs with the McLeod phenotype from RBCs with normal Kell antigen expression. Crisp mixed-field agglutination was obtained in tests with monoclonal anti-K14 versus RBCs from the 12 obligate carrier females. Flow cytometry using anti-K14 also clearly identified two RBC populations (median

Ragnhild Øyen, Marion E. Reid, Pablo Rubinstein, Harold Ralph

Immunohematology, Volume 12 , ISSUE 4, 160–163

Article | 16-February-2021

Comparison of ABO genotyping methods: a study of two low-resolution polymerase chain reaction assays in a clinical testing laboratory

J.A. Keller, T. Horn, S. Scholz, S. Koenig, M.A. Keller

Immunohematology, Volume 35 , ISSUE 4, 149–153

Article | 16-October-2019

Assessment of common red blood cell pretreatments to yield an accurate serologic antigen phenotype compared with genotype-predicted phenotype

been sourced, increasing the complexity because of the lower incidence of the e– phenotype. If this testing were to be used to provide antigen-matched blood, it could potentially cause delays in blood selection because of the perceived need for more antigens to be negative than is needed. This study shows the advantages of using genotyping to predict RBC antigen expression and confirms that it is preferable in difficult patient samples. Our results show that RBC manipulation can result in serologic

T. Horn, J. Hamilton, J. Kosanke, V.W. Hare, W. Kluver, W. Beres, S. Nance, M.A. Keller

Immunohematology, Volume 33 , ISSUE 4, 147–151

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