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  • Immunohematology

 

Article | 18-October-2020

A gel technology system to determine postpartum RhIG dosage

Failures of Rh immune globulin (RhIG) prophylaxis occur when the dose is too small. We report a test using a gel technology (GT) method to replace the Kleihauer–Betke (K–B) test to assess fetomaternal hemorrhage (FMH) and assist in determining the minimum necessary dose of RhIG. Cord blood (O, D+) was mixed with adult blood (O D–) to mimic an FMH of 10 mL, 20 mL, 28 mL, and 40 mL. Test samples were incubated with anti-D at known concentrations and centrifuged. The supernatant

John R. Fernandes, Ronny Chan, Ahmed S. Coovadia, Marciano D. Reis, Peter H. Pinkerton

Immunohematology, Volume 16 , ISSUE 3, 115–119

Article | 14-October-2020

Comparison of three low-ionic diluents for dilution and storage of reagent A1 and B cells for testing in gel technology

Currently, ABO serum grouping performed by gel technology employs a red cell diluent containing EDTA (MTS Diluent 2 Plus™) that does not permit extended storage of the red cell suspensions. A diluent currently used for suspension and long-term storage of reagent red cells for antibody detection and identification (Ortho 0.8% Red Cell Diluent™) was evaluated for use with A1 and B cells. Because this diluent does not contain EDTA, testing was limited to EDTA samples. As a comparison

E. Ann Steiner, LouAnn Dake

Immunohematology, Volume 17 , ISSUE 2, 53–56

Letter to Editor | 18-October-2020

Letters to the Editors Re: Gel technology for RhIG dosage

Stephen Apfalroth

Immunohematology, Volume 16 , ISSUE 4, 161–161

Article | 14-October-2020

Comparative testing for weak expression of D antigen: manual tube testing vs. a semiautomated IgG gel system

Bill A. Martinez, Liz A. Crews, Arlene M. Dowd, Melissa McMahan

Immunohematology, Volume 19 , ISSUE 1, 7–9

Article | 03-November-2020

Comparison of gel technology and red cell affinity column technology in antibody detection

Sauvai I. Chanfong, Sherri Hill

Immunohematology, Volume 14 , ISSUE 4, 152–154

Article | 01-April-2020

Application of gel technology in the serologic characterization of autoantibody in DAT-positive autoimmune diseases

Sudipta Sekhar Das, Rajendra K. Chaudhary

Immunohematology, Volume 23 , ISSUE 2, 59–62

Report | 29-October-2019

Demonstration of IgG subclass (IgG1 and IgG3) in patients with positive direct antiglobulin tests

hemolysis when IgG1 or IgG1-IgG3 both were present. Gel technology is helpful to demonstrate red blood cell–bound autoantibodies and their characterization with regard to class, subclass, and titer. This information is useful to identify patients with AIHA who are at risk of severe hemolysis with adverse prognosis.

Ashutosh Singh, Archana Solanki, Rajendra Chaudhary

Immunohematology, Volume 30 , ISSUE 1, 24–27

Article | 27-April-2020

On a much higher than reported incidence of anti-c in R1R1 patients with anti-E

A previous study involving tube IATs, untreated RBCs, and a lowionic-strength additive reagent revealed that approximately onethird of R1R1 patients with anti-E have a concomitant anti-c. However,the current study finds a much higher incidence of anti-c in such patients, using gel technology in conjunction with ficinpretreated RBCs. Results of antibody identification studies and transfusion records of 82 R1R1 patients with anti-E were reviewed. Serologic test methods included a LISS wash

W. John Judd, Louann R. Dake, Robertson D. Davenport

Immunohematology, Volume 21 , ISSUE 3, 94–96

Report | 01-December-2019

Comparison of estimation of volume of fetomaternal hemorrhage using KleihauerBetke test and microcolumn gel method in D-negative nonisoimmunized mothers

volume of FMH in the remaining 18 (22.5%) cases by KB was 8.3 ± 1.7 mL. Fifteen (83.3%) of these 18 cases had FMH of at least 0.4 percent (approximately 8 mL) by gel technology. Three cases (16.7%) that differed from KB results had FMH of 0.2 percent by microcolumn gel method with a maximal FMH of 6.4 mL by KB. FMH was significantly increased in cesarean delivery (mean FMH 9.5 ± 0.8 mL, range 7.9–10.4 mL, p = 0.001) and antepartum hemorrhage (mean FMH 9.5 ± 0.9 mL, range

Kshitija Mittal, Neelam Marwaha, Praveen Kumar, Subhash C. Saha, Beenu Thakral

Immunohematology, Volume 29 , ISSUE 3, 105–109

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