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Article | 15-April-2020

Chimerism and mosaicism are important causes of ABO phenotype and genotype discrepancies

Discrepancies between blood group genotype and RBC phenotype are important to recognize when implementing DNA-based blood grouping techniques. This report describes two such cases involving the ABO blood group in the Korean population. Propositus #1 was a 22-year-old healthy man undergoing pretransfusion testing for minor surgery. Propositus #2 was a 23year-old male blood donor. RBCs from both propositi were determined to be group AB and demonstrated unusual agglutination patterns on forward

Duck Cho, Jin Sol Lee, Mark Harris Yazer, Jong Won Song, Myung Geun Shin, Jong Hee Shin, Soon Pal Suh, Mee Jeong Jeon, Ji Young Kim, Jong Tae Park, Dong Wook Ryang

Immunohematology, Volume 22 , ISSUE 4, 183–187

Article | 03-November-2020

Evaluation of the GTI-ASP-1 platelet antigen genotyping kit for the determination of the HPA-1 genotype

Tobias J. Legler, Christopher Hagner, Michael Köhler

Immunohematology, Volume 14 , ISSUE 1, 19–21

original-paper | 22-October-2019

Molecular Epidemiology of Hepatitis B Virus in Turkish Cypriot

colonization, the dominance of genotype D/D3 is observed (Paoli et al. 2018). A similar study was performed before in the same region, and once again, genotype D/D3 was found to be the most relevant genotype (Chacha et al. 2017). The prevalence of HBV can be classified into three regions; low (< 2%), middle (2–7%), and high (> 8%) endemities. Turkey is categorized as middle endemicity with a prevalence rate of 0.8–5.7%, while the Turkish Republic of Northern Cyprus (TRNC) falls into a low category with a


Polish Journal of Microbiology, Volume 68 , ISSUE 4, 449–456

Short Communication | 30-March-2017

Use of Amplification Fragment Length Polymorphism to Genotype Pseudomonas stutzeri Strains Following Exposure to Ultraviolet Light A

Changes in ultraviolet light radiation can act as a selective force on the genetic and physiological traits of a microbial community. Two strains of the common soil bacterium Pseudomonas stutzeri, isolated from aquifer cores and from human spinal fluid were exposed to ultraviolet light. Amplification length polymorphism analysis (AFLP) was used to genotype this bacterial species and evaluate the effect of UVA-exposure on genomic DNA extracted from 18 survival colonies of the two strains

Lisa Lombardi, Marina Zoppo, Cosmeri Rizzato, Colin Gerard Egan, Roberto Scarpato, Arianna Tavanti

Polish Journal of Microbiology, Volume 66 , ISSUE 1, 107–111

Article | 16-October-2019

Assessment of common red blood cell pretreatments to yield an accurate serologic antigen phenotype compared with genotype-predicted phenotype

EGA treatment in removing IgG from RBCs. This study aimed to compare the accuracy of the results obtained from the commonly used RBC pretreatment methods with the results from a genotype-predicted phenotype using the BeadChip platform. Table 1. Blood group antigens predicted by the HEA BeadChip genotyping panel Blood group system Antigens RH C, c, E, e, V, VS FY Fya, Fyb, Fyb silenced DO Doa, Dob, Hy, Joa SC Sc1, Sc2 DI Dia, Dib LW LWa, LWb CO Coa, Cob JK Jka, Jkb KEL

T. Horn, J. Hamilton, J. Kosanke, V.W. Hare, W. Kluver, W. Beres, S. Nance, M.A. Keller

Immunohematology, Volume 33 , ISSUE 4, 147–151

Article | 14-October-2020

Blood group antigen profile predicted by molecular biology - use of real-time polymerase chain reaction to genotype important KEL, JK, RHD, and RHCE alleles

-genotype correlations were obtained. The potential use of the presented methods can be predicted in clinical transfusion medicine,allowing appropriate monitoring, early intervention, and improved care. When blood group genotyping techniques are necessary, this methodology is highly competitive for a routine laboratory.

Fernando Manuel Ferreira Araújo, Christiana Pereira, Fátima Monteiro, Isabel Henriques, Elsa Meireles, Pedro Lacerda, Ana Aleixo, Regina Celeste, Luis M. Cunha-Ribeiro, Maria J. Rodrigues

Immunohematology, Volume 18 , ISSUE 3, 59–64

research-article | 30-November-2018

Screening of Cucurbita maxima and Cucurbita moschata Genotypes for Resistance Against Meloidogyne arenaria, M. incognita, M. javanica, and M. luci

ovule culture (Kurtar et al., 2018) (Table 1). Seeds were placed on wet filter paper in Petri dishes and germinated for 3 days at 24 ± 2°C in the dark. Pre-germinated seeds were sown singly in 300 ml pots filled with sterilized sandy soil. Pots were maintained in a plant growth room at 24 ± 2°C with a 16 hr/8 hr (light/dark) cycle. Two weeks after sowing, the seedlings were inoculated with 5000 eggs (Pi) at the second true leaf stages. Each RKN species-plant genotype combination was replicated four

Gökhan Aydınlı, Ertan Sait Kurtar, Sevilhan Mennan

journal of nematology, Volume 51 , 1–10

short-communication | 30-November-2018

Comparison of Performance Characteristics of DxN VERIS System versus Qiagen PCR for HBV Genotype D and HCV Genotype 1b Quantification

striking differences in the amount final product. These reasons indicate the necessity of inventing fully standardized, reproducible and sensitive new assays for monitoring of HBV and HCV infections. The aim of the study was to compare the analytical performance of random access testing (Beckman Coulter DxN VERIS) with routine diagnostic PCR kit (artus Qiagen). A total of 67 chronic hepatitis B (CHB) patients with genotype D and 44 chronic hepatitis C (CHC) patients infected with genotype 1b from


Polish Journal of Microbiology, Volume 68 , ISSUE 1, 139–143

Review | 17-March-2020

The ABO blood group system revisited: a review and update

Jill R. Storry, Martin L. Olsson

Immunohematology, Volume 25 , ISSUE 2, 48–59

Article | 26-October-2019

Mass-scale donor red cell genotyping using real-time array technology

blood donors to find rare phenotypes and rare combinations of antigens. When performed on donors who are predicted to donate again after testing, integrating the genotype information with existing donor data and demographics provides the blood center with real-time information to identify the common clinically relevant blood group antigens demanded by hospital transfusion services. This review outlines a red cell genotype methodology using TaqMan chemistry and existing algorithms and data handling

Gregory A. Denomme, Michael J. Schanen

Immunohematology, Volume 31 , ISSUE 2, 69–74

Research Article | 26-September-2018

Oat, Wheat, and Sorghum Genotype Reactions to Meloidogyne incognita and Meloidogyne javanica


Journal of Nematology, Volume 49 , ISSUE 4, 386–389

Article | 26-October-2019

An overview of the Progenika ID CORE XT: an automated genotyping platform based on a fluidic microarray system

highly automated and includes integrated analysis software that produces a file and a report with genotype and predicted phenotype results.

Mindy Goldman, Núria Nogués, Lilian M. Castilho

Immunohematology, Volume 31 , ISSUE 2, 62–68

research-article | 23-April-2019

Fusarium wilt of cotton may commonly result from the interaction of Fusarium oxysporum f. sp. vasinfectum with Belonolaimus longicaudatus

manufacturer’s instructions. A 24 μl sample of 40–68 ng/µl of PCR product and 12 μl of each 3 μM primer were sent to Quintara Biosciences (2600 Hilltop Dr, Building B, R332, Richmond, CA) for sequencing. Sequences were manually checked and aligned with known Fov sequences from GenBank database and private database to identify race or genotype. For 2016 samples, Fov was isolated by washing cotton stems in soapy water, then surface sanitizing for 1 min in 95% ethanol, 2 min in sodium hypochlorite solution and

Mychele B. da Silva, Richard F. Davis, Hung K. Doan, Robert L. Nichols, Robert C. Kemerait, Hannah C. Halpern, Marin T. Brewer, Ganpati Jagdale, Peng W. Chee

Journal of Nematology, Volume 51 , 1–10

Review | 01-December-2019

Immunosuppressive protocols for transplantation and certain hematologic malignancies can prevent the primary immune response to the D blood group antigen

A review of the published literature on Rh alloimmunization reveals that its incidence varies with the volume of infused D+ red blood cells (RBCs), the probable Rh genotype of the RBCs, and the immune competency of the D– recipient. Among the reports of Rh alloimmunization in different clinical circumstances, we identified five studies in which a combined total of 62 D– recipients of hematopoietic stem cell or solid-organ transplants were transfused with D+ RBCs and none (0%) formed

Adair Seager, S. Gerald Sandler

Immunohematology, Volume 29 , ISSUE 3, 110–114

Article | 08-April-2018

Analysis and Study the Correlation of Genetic Loci

numerical language, and finally got the image of the corresponding computing program operation and results. By carrying out statistical analysis and inspection for results, the score genotype data are more reasonable and accurate to explain theoretically the rationality of the discovered disease locus and gene.

Liu Yang1,, Bai Xiaojun2, Kang Xiaofeng3

International Journal of Advanced Network, Monitoring and Controls, Volume 1 , ISSUE 2, 118–124

Report | 12-March-2020

Consortium for Blood Group Genes (CBGG): 2009 report

involving blood group genetics. This year witnessed a change in the standing committee membership and the institution of a representative for the human platelet antigens group. Looking forward, the consortium sees challenges for the nomenclature of blood group alleles and user-required specifi cations for laboratory information systems to store genotype information.

Gregory A. Denomme, Connie M. Westhoff, Lilian Maria de Castilho, Maryse St-Louis, Vagner Castro, Marion E. Reid

Immunohematology, Volume 26 , ISSUE 2, 47–50

Article | 15-April-2020

Molecular characterization of GYPB and RH in donors in the American Rare Donor Program

Transfusion of patients with sickle cell disease (SCD) has been a challenge in clinical transfusion medicine, especially when the required donor RBCs must be U– and negative for high-prevalence Rh phenotypes (hrB,hrS). It is now possible to genotype donors to identify or confirm Uvar and U– phenotypes,as well as Rh hrB– and hrS– phenotypes,and to characterize the different RH backgrounds found in these donors. In a preliminary study of donors registered in the American

Sunitha Vege, Connie M. Westhoff

Immunohematology, Volume 22 , ISSUE 3, 143–147

Article | 20-April-2020

Expression of Duffy antigen receptor for chemokines during reticulocyte maturation:using a CD71 flow cytometric technique to identify reticulocytes

to determine the expression of Fy on CD71-positive and -negative reticulocytes and to correlate serology and genotype. A reduction of 13 percent was seen in Fy6 expression between CD71-positive reticulocytes and RNA-positive reticulocytes. CD71 disappears early during reticulocyte maturation, while Fy6 expression is relatively preserved. CD71 is an alternative to staining for RNA for reticulocyte assays relating to Fy6 expression.

Ian J. Woolley, Erica M. Wood, R. Michael Sramkoski, Peter A. Zimmerman, John P. Miller, James W. Kazura

Immunohematology, Volume 21 , ISSUE 1, 15–20

original-paper | 27-December-2020

Identification and Characterization of a Novel Recombinant Porcine Astrovirus from Pigs in Anhui, China


Polish Journal of Microbiology, Volume 69 , ISSUE 4, 471–478

Article | 21-July-2017

Reproduction and Damage Potential of Five Geographical Ditylenchus africanus Populations on Peanut

. africanus geographic populations from five different areas in the peanut production area of South Africa and to assess whether PC254K1 is resistant to all five D. africanus populations. Reproduction of the D. africanus populations was evaluated on peanut callus in growth cabinets at 218C, 288C, and 358C. The peanut cv. Sellie was included in the study as the D. africanus-susceptible reference genotype in the greenhouse and microplots. Reproduction potential of all five of the D


Journal of Nematology, Volume 48 , ISSUE 2, 72–78

research-article | 30-November-2020

Mycobacterium chimaera as An Underestimated Cause of NTM Lung Diseases in Patients Hospitalized in Pulmonary Wards

positive respiratory cultures for M. intracellulare or MAC. Experimental Materials and Methods Respiratory strains of either M. intracellulare or MAC, identified from 2011 through 2020 by the Geno-Type CM test (Hain Lifescience) in various respiratory wards, have been re-evaluated with GenoType NTM-DR test (Hain Lifescience). This method identifies various MAC species via the DNA-STRIP method, analyzing the 23S rRNA gene polymorphism. The data concerning the number of specimens tested and the number


Polish Journal of Microbiology, Volume 70 , 1–6

Report | 01-December-2019

Validation of a blood group genotyping method based on high-resolution melting curve analysis

obtained genotype distribution is in complete concordance with existing data for the Chinese population. There are several advantages for this approach of blood group genotyping: lower contamination rates with PCR products in the laboratory, ease of performance, automation potential, and rapid cycling time.

Tianxiang Gong, Ying Hong, Naihong Wang, Xuemei Fu, Changhua Zhou

Immunohematology, Volume 30 , ISSUE 4, 161–165

Article | 20-April-2020

FCGR3B polymorphism in three ethnic Chinese populations

by PCR using sequence specific primers (PCR-SSP). The results showed the gene frequencies were 0.55 for FCGR3B*1 and 0.45 for FCGR3B*2 in 177 Han individuals,0.69 for FCGR3B*1 and 0.31 for FCGR3B*2 in 87 She individuals,and 0.35 for FCGR3B*1 and 0.65 for FCGR3B*2 in 99 Tajik individuals,respectively. The FCGR3Bnull genotype was not found,but the FCGR3B*3 allele was identified in only three individuals in the Tajik population. DNA clone and sequencing confirmed that these individuals had the C

Lixing Yan, Faming Zhu, Lei Jin, Qinfeng Lv, Qihua Fu

Immunohematology, Volume 21 , ISSUE 1, 25–28

research-article | 31-December-2019

Gallbladder diseases in pregnancy: Sonographic findings in an indigenous African population

institutional board approved the study. All the participants were recruited consecutively after informed consent had been obtained. Only asymptomatic and clinically stable women were enrolled. Biodata was obtained by oral interviews. Hemoglobin genotype and blood group were retrieved from the subjects’ antenatal clinic cards. Siemens ultrasound scanner model GM-6800A2E00 (Siemens AG, Erlangen, Germany) with a convex transducer (frequency range = 3.5–6.0 MHz) was used for the sonographic examinations

Bukunmi Michael Idowu, Stephen Olaoluwa Onigbinde, Isaiah Uzezi Ebie, Michael Temidayo Adeyemi

Journal of Ultrasonography, Volume 19 , ISSUE 79, 269–275

Case report | 01-December-2019

Molecular RH blood group typing of serologically D–/CE+ donors: the use of a polymerase chain reaction–sequence-specific primer test kit with pooled samples

genotyped samples, 12 were found to be PCR positive (5.1%), exhibiting DEL genotype and RHD-CE-D hybrid alleles. Our data demonstrate that the use of a PCR-SSP commercial test kit with pooled samples is a helpful and valid method to correctly detect RHD alleles. As a consequence, we reclassified our donors as carriers of potentially immunogenic alleles.

Donatella Londero, Mauro Fiorino, Valeria Miotti, Vincenzo de Angelis

Immunohematology, Volume 27 , ISSUE 1, 25–28

Case report | 14-October-2020

Neonatal alloimmune thrombocytopenia due to HPA-3a antibodies: a case report

commerical antigencapture ELISA (GTI-PakPlus kit®). Anti-HPA-3a antibodies, while weakly reactive in the monoclonal antibody immobilization of platelet antigens (MAIPA) assay in the immediate postpartum serum, were readily detectable using this assay in a sample taken 4 weeks later. Genotyping for human platelet antigens (HPA) 1–5 by the polymerase chain reaction technique with sequence-specific primers (PCR-SSP) revealed the infant’s platelet genotype to be HPA-1a/1a, 3a/3b, while that

A. Davoren, G. Smith, G. Lucas, S. Rodgers, P. O’Donoghue, J. Crowley, C.A. Barnes, J. McKiernan

Immunohematology, Volume 18 , ISSUE 2, 33–36

Case report | 14-October-2020

Moderate hemolytic disease of the newborn (HDN) due to anti-Rh17 produced by a black female with an e variant phenotype

cause mild to fatal HDN. We report an example of anti-Rh17 produced by a black female with an e variant RBC phenotype that caused moderate HDN. A panel of seven monoclonal anti-e demonstrated her RBCs carried a variant e antigen, and her genotype was RHD, RHce by PCR-RFLP analysis. Amniotic fluid with ΔOD450 values from 30 to 35 weeks’ gestation predicted moderate HDN probability by the Liley method. At 38+ weeks, a viable 3165 g female infant was delivered. The infant’s direct

Marla C. Brumit, Gary E. Carnahan, James R. Stubbs, Jill R. Storry, Marion E. Reid

Immunohematology, Volume 18 , ISSUE 2, 40–42

Research paper | 31-July-2017

Analysis of methionine synthase (rs1805087) gene polymorphism in autism patients in Northern Iran

this study was to analyze the association of MTR A2756G gene polymorphism (rs1805087) and the risk of autism in a population in northern Iran. The prevalence of MTR A2756G polymorphism was determined in 108 children with autism and 130 controls in northern Iran. Genotypes and allele frequencies were determined in patients and controls by polymerase chain reaction‑restriction fragment length polymorphism (PCR‑RFLP). The prevalence of genotype frequencies of AA, AG and GG in autistic children were

Rosa Haghiri, Farhad Mashayekhi, Elham Bidabadi, Zivar Salehi

Acta Neurobiologiae Experimentalis, Volume 76 , ISSUE 4, 318–323

original-paper | 27-March-2019

Interferon Gamma Release Assays in Patients with Respiratory Isolates of Non-Tuberculous Mycobacteria – a Preliminary Study

centrifuged at 3000 × g for 15 min. The pellet was suspended in 2 ml of phosphate buffer. The strains were cultured on solid media: egg-based L-J medium, Stonebrink medium and in automated system MGIT (Becton Dickinson) (Klatt et al. 2015). Identification of culture was performed using the niacin test and a molecular method GenoType CM test (Hain Lifescience). The GenoType CM test, using the DNA-STRIP method allowed the identification of M. tuberculosis complex strains and 14 clinically relevant NTM


Polish Journal of Microbiology, Volume 68 , ISSUE 1, 15–19

Original Paper | 28-December-2016

Genotyping and Clinicoepidemiological Characterization of Rotavirus Acute Gastroenteritis in Egyptian Children

and emergence of G9 RVA highlight the need to apply vaccines against this genotype in Egypt.

Niveen Saudy, Walaa Othman Elshabrawy, Ahmed Megahed, Mona F. Foad, Aly F. Mohamed

Polish Journal of Microbiology, Volume 65 , ISSUE 4, 433–442

Original Paper | 04-December-2017

Study of Patterns and Markers of Human Immune Deficiency Virus -1 (HIV-1) Progression and Unemployment Rate among Patients from Alexandria, Egypt

Middle East and North Africa (MENA) new HIV cases show the highest increase among all regions in the world. Even though Egypt has a low prevalence among the general population (< 0.02%), a national HIV epidemic occurs in certain population risk groups. The current study was conducted to asses clinical and immunological disease progression; following up viral load (VL) and detecting delta-32 CCR5 genotype polymorphism in selected cases, determining unemployment rate and identify predictors of

Faika M. Ghoneim, May M. Raouf, Noha S. Elshaer, Sarah M. Abdelhamid, Reem A. Noor Eldeen

Polish Journal of Microbiology, Volume 66 , ISSUE 4, 519–527

Case report | 16-March-2020

Autoantibody formation after alloimmunization inducing bystander immune hemolysis

was present in the eluate, and the antibody investigation revealed the presence of anti-Jkb in addition to the previously identified alloanti-c. Her genotype was determined, and, based on the findings, two additional units were selected, found to be compatible, and transfused without incident. Transfusions were discontinued, and she was treated with IVIG and corticosteroids. Her Hb increased to 9.7 g/dL, and the patient made an uneventful recovery. It was concluded that transfusion of incompatible

Mariza Mota, C. Bley, M.G. Aravechia, N. Hamerschlak, A. Sakashita, J.M. Kutner, L. Castilho

Immunohematology, Volume 25 , ISSUE 1, 9–12

Research Article | 03-December-2018

Broad-based root-knot nematode resistance identified in cowpea gene-pool two

%). Resistant genotypes had more effective resistance than the Rk-based resistance in cowpea genotype CB46 against Mj and Mi. Root-galling was correlated across isolates (Avr-Mi/Mj: r = 0.72; Mi/Mj: r = 0.98), and RG was correlated with EM (r = 0.60), indicating resistance to RG and EM is under control by the same genetic factors. These new sources of resistance identified in cowpea gene-pool two provide valuable target traits for breeders to improve cowpea production on RKN-infested fields.

Arsenio D. Ndeve, William C. Matthews, Jansen R. P. Santos, Bao Lam Huynh, Philip A. Roberts

Journal of Nematology, Volume 50 , ISSUE 4, 545–558

Report | 01-December-2019

Molecular background of RH in Bastiaan, the RH:–31,–34 index case, and two novel  RHD alleles

were weakly reactive or nonreactive, MS21 was strongly reactive, and HIRO38, HIRO41, and HIRO43 were nonreactive. Results show that Bastiaan has RHD*DIIIa150C and RHD*DIIIa-CE(4-7)-D. Tests on 605 samples from random African Americans revealed a frequency of 0.036 for RHCE*ce 48C, 733G, 1006T and revealed two novel alleles: RHD*186T and RHD*DIIIa 150C. The Bastiaan phenotype is encoded by RHD*DIIIa150C-RHCE*ce 48C, 733G, 1006T and RHD*DIIIa-CE(4-7)-D-RHCE*ce 48C, 733G, 1006T; thus, this genotype is

Marion E Reid, Christine Halter Hipsky, Randall W. Velliquette, Christine Lomas-Francis, Kathleen Larimore, Coral Olsen

Immunohematology, Volume 28 , ISSUE 3, 97–103

Report | 12-March-2020

Application of real-time PCR and melting curve analysis in rapid Diego blood group genotyping

blood donor was found to be homozygous for DI*01 in this study. The calculated DI*01 and DI*02 allele frequencies were 0.0181 (95% CI, 0.0173–0.0189) and 0.9819 (95% CI, 0.9791–0.9847), respectively, showing a good fit for the Hardy-Weinberg equilibrium. There was full concordance among Diego phenotype results and Diego genotype results by PCR-SSP and real-time PCR. DI*01 and DI*02 allele determination with SYBR Green I and thermal cycler technology are useful methods for Diego

Marcia C. Zago Novaretti, Azulamara da Silva Ruiz, Pedro Enrique Dorlhiac-Llacer, Dalton Alencar Fisher Chamone

Immunohematology, Volume 26 , ISSUE 2, 66–70

Report | 26-October-2019

First example of an FY*01 allele associated with weakened expression of Fya on red blood cells

from the proband’s parents indicated that the father had the same FY genotype as the fetus. Flow cytometry, which has been previously demonstrated as a useful method to study antigen strength on cells, was used to determine if this new FY*01 allele was associated with reduced Fya expression on the father’s RBCs. Median fluorescence intensity of the father’s RBCs (after incubation with anti-Fya and fluorescein-labeled anti-IgG) was similar to known FY*01 heterozygotes and

Patricia A. Arndt, Trina Horn, Jessica A Keller, Rochelle Young, Suzanne M. Heri, Margaret A. Keller

Immunohematology, Volume 31 , ISSUE 3, 103–107

Review Paper | 07-April-2017

Genetic epilepsies. remarks on the proposed “organization of the Epilepsies”

, electroclinical syndromes and their genetics and genetic testing in the epilepsies. Methods. The updated review is based on OMIM™ (Online Mendelian Inheritance in Man). Review and remarks. Because of the vast genetic and phenotypic heterogeneity, bridging genotype and phenotype remains a major challenge in epilepsy genetics. The so-called “idiopathic” epilepsies are genetically determined. The new ILAE proposal on the “organization” of the epilepsies takes into account the

Heinz Gregor Wieser

Journal of Epileptology, Volume 22 , ISSUE 1, 11–23

Original Paper | 09-October-2019

Use of standard laboratory methods to obviate routine dithiothreitol treatment of blood samples with daratumumab interference

Daratumumab is an antibody currently used in the treatment of patients with refractory multiple myeloma. Blood samples from patients being treated with daratumumab may show panreactivity during pre-transfusion testing. To facilitate the provision of blood components for such patients, it is recommended that a baseline phenotype or genotype be established prior to starting treatment with daratumumab. If patient red blood cells (RBCs) require phenotyping after the start of daratumumab treatment

Nicholas J. Lintel, Debra K. Brown, Diane T. Schafer, Farai M. Tsimba-Chitsva, Scott A. Koepsell, Sara M. Shunkwiler

Immunohematology, Volume 33 , ISSUE 1, 22–26

Article | 17-February-2021

A resource-conserving serologic and high-throughput molecular approach to screen for blood donors with an IN:−5 phenotype

assay was devised to identify the frequency of the allele (IN*02.–05; p.150His) encoding the INRA– phenotype (IN:−5).10 Primers 5´-GCGGGCCTCTCTCCCAGCTATTGTTAACCA-3´ and 5´-ATTCTCCTTTCTGGACATAGCGGG-3´ were designed to genotype c.449G>A (p.Arg150His; rs771323886) single nucleotide variation (SNV) in exon 5 of the CD44 gene and applied to genomic DNA (gDNA). Controls included in each plate were heterozygous for c.449G>A from the children of the proband (INRA, IN:−5), homozygous for c.449G from a random

S.R. Joshi, S.B. Senjaliya, K. Srivastava, W.A. Flegel

Immunohematology, Volume 36 , ISSUE 4, 129–132

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