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Report | 01-December-2019

Validation of a blood group genotyping method based on high-resolution melting curve analysis

The detection of polymorphism is the basis of blood group genotyping and phenotype prediction. Genotyping may be useful to determine blood groups when serologic results are unclear. The development and application of different methods for blood group genotyping may be needed as a substitute for blood group typing. The purpose of this study is to establish an approach for blood group genotyping based on a melting curve analysis of realtime polymerase chain reaction (PCR). Using DNA extracted

Tianxiang Gong, Ying Hong, Naihong Wang, Xuemei Fu, Changhua Zhou

Immunohematology, Volume 30 , ISSUE 4, 161–165

Report | 12-March-2020

Application of real-time PCR and melting curve analysis in rapid Diego blood group genotyping

The paucity of appropriate reagents for serologic typing of the Diego blood group antigens has prompted the development of a real-time PCR and melting curve analysis for Diego blood group genotyping. In this study, we phenotyped 4326 donor blood samples for Dia using semiautomated equipment. All 157 Di(a+) samples were then genotyped by PCR using sequence-specific primers (PCR-SSP) for DI*02 because of anti-Dib scarcity. Of the 4326 samples, we simultaneously tested 160 samples for Dia and Dib

Marcia C. Zago Novaretti, Azulamara da Silva Ruiz, Pedro Enrique Dorlhiac-Llacer, Dalton Alencar Fisher Chamone

Immunohematology, Volume 26 , ISSUE 2, 66–70

research-article | 30-November-2019

Molecular characterization of the Pratylenchus vulnus populations on cereals in Turkey

3 min at 95°C, 35 cycles of amplification were DNA denaturation for 10 sec at 95°C, annealing for 60 sec at 63°C, and extension for 30 sec at 72°C. DNA melting curve analysis of the amplicon was performed by increasing the temperature from 72 to 95°C at 0.2 to 0.5°C/sec (ramp). The fluorescent signal was measured using the FAM or SYBR/FAM channel, after every cycle and after every temperature increment of the PCR melting curve. Sequence alignment Sequence alignments of one of the P. vulnus

Mehmet Sait Karaca, Elif Yavuzaslanoglu, Gul Imriz, Ozlem Ates Sonmezoglu

Journal of Nematology, Volume 52 , 1–4

Review | 20-March-2020

Detection and identification of platelet antibodies and antigens in the clinical laboratory

antibody tests. Fueled by development of PCR and determination of the molecular basis of the PlA1 human platelet antigen (HPA), serologic platelet typing has now been replaced by genotyping of DNA. Allele-specific PCR, melting curve analysis, and 5′-nuclease assays are now evolving into more high-throughput molecular tests. Laboratory testing for the diagnosis of immune platelet disorders has advanced considerably from its humble beginnings.

Brian R. Curtis, Janice G. McFarland

Immunohematology, Volume 25 , ISSUE 3, 125–135

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