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Report | 01-December-2019

Effects of pH changes of stock normal saline solution on 5 percent red cell suspension

The red cell suspension (RCS) is a universally used indicator system to demonstrate antigen and antibody reactions in vitro. Saline solutions that are used in its preparation are preferred to be fresh to avoid changes in pH that may affect the results. Thus, buffered saline such as phosphate-buffered saline (PBS) is the ideal diluent because its pH is maintained for a certain period. However, normal saline solution (NSS) is more commonly used because it is inexpensive and easy to make. pH

Gregorio L. Martin, Princess Joy M. Caraan, Jared Jenrik S. Chua, Jules Albert L. Crescini, Joseph Martin C. Diokno, Christopher Bernard Dlr. Javier, Kristina Barbara O. Reyes, Rianne Y. Soliven

Immunohematology, Volume 30 , ISSUE 3, 126–134

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