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  • Immunohematology

 

Report | 14-March-2020

Attempts to support an immune etiology in 800 patients with direct antiglobulin test–negative hemolytic anemia

antiglobulin sera (AGS) panel of anti-IgG, anti-C3, anti-IgM, and anti-IgA by a routine DAT. Additional tests included a direct Polybrene test to detect small amounts of RBCbound IgG, a cold-wash technique to detect low-affinity IgG, and a DAT by gel test with anti-IgG. A positive result was obtained with at least one method for 431 (54%) of 800 specimens tested. The AGS panel was positive for 400 (50%) of samples, with IgG or C3 or both accounting for reactivity in 48 percent. IgA alone was found on 2

Regina M. Leger, Asuncion Co, Penny Hunt, George Garratty

Immunohematology, Volume 26 , ISSUE 4, 156–160

Article | 14-October-2020

Fatal hemolytic transfusion reaction due to anti-Ku in a Knull patient

A fatal transfusion reaction due to anti-Ku in a Knull (Ko) patient is reported. The patient was transfused with 34 units of incompatible RBCs during 44 days of hospitalization. Apart from the first transfusion, all subsequent transfusions failed to raise the patient’s Hb. No serum antibody was identified until he was transferred to another hospital for dialysis. A compatibility test demonstrated a weak antibody and autocontrol reacting at room temperature by a manual polybrene method

Marie Lin, Chang Lin Wang, Fu-Sen Chen, Li-Hwa Ho

Immunohematology, Volume 19 , ISSUE 1, 19–21

Case report | 29-December-2020

Evaluation of a complement-dependent anti-Jka by various sensitization and detection methodologies: a case report

A 79-year-old woman with a diagnosis of lower gastrointestinal bleeding was found to have a complement-dependent anti-Jka in her serum. The anti-Jka was evaluated by the antiglobulin technique with polyspecific, anti-C3, and anti-IgG antihuman globulin (AHG). A variety of sensitization and detection methods were used, including the prewarmed saline technique, enzyme treatment of test cells, a low-ionic additive solution (LISS), 22 percent albumin, Polybrene, and an increased serum/cell ratio

E. Nicole DeLong

Immunohematology, Volume 4 , ISSUE 3, 59–63

Article | 30-November-2020

An example of anti-LWa in a 10-month-old infant

Blood samples from a 10-month-old male infant requiring transfusion were found to contain an allomtibody reacting at 37°C in saline, by indirect antiglobulin test (IAT), and with a manual polybrene technique. Preliminary results suggested anti-D and another weaker reacting antibody, but the patient had been previously transfused with only D- blood. His serum reacted more weakly by IAT against red cells treated with 0.2M dithiothreitol (DTT), and one D+, LW(a-) sample was nonreactive. The

Alan Devenish

Immunohematology, Volume 10 , ISSUE 4, 127–129

Article | 30-November-2020

Hemolytic transfusion reactions due to anti-e+f detectable only by nonstandard serologic techniques

. The patient’s RBCs typed as A, D+, probable Rh phenotype (cDE/cDE). The direct antiglobulin test was negative, and serum samples following the second transfusion were red/brown in color. Serologic investigations were inconclusive on all samples taken until February 13 (after the fourth transfusion). At this time, a weak anti-e reacting by manual polybrene technique and an anti-e+f reacting by two-stage papain technique were detected. The serum also contained potent HLA antibodies. The

Alan Devenish, Lesley A. Kay

Immunohematology, Volume 10 , ISSUE 4, 120–123

Article | 18-October-2020

From kill to overkill: 100 years of (perhaps too much) progress

Peter D. Issitt

Immunohematology, Volume 16 , ISSUE 1, 18–25

Article | 10-November-2020

A new form of polyagglutination related to Cad

;C, 20°C, 37°C, and anti-human globulin test), and was inhibitable by two examples of Sd(a+) urine, but not by Sd(a–) urine or dialysate from Sd(a+) urine. The donor’s RBCs reacted 1+ with Glycine max, but did not react with Dolichos biflorus, Leonurus cardiaca, Salvia horminum, or Arachis hypogaea. The first four of these lectins were reactive with five of five Cad+ RBCs, including one example of Cad 4 RBCs. Polybrene® aggregated the donor RBCs. Dilutions of nine samples

Regina M. Leger, Elfreda J. Lines, Keith Cunningham, George Garratty

Immunohematology, Volume 12 , ISSUE 2, 69–71

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