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  • Immunohematology


Article | 26-October-2020

Modern approaches to pretransfusion testing

W. John Judd

Immunohematology, Volume 15 , ISSUE 1, 41–52

Article | 26-October-2020

Precipitation of serum proteins by polyethylene glycol (PEG) in  pretransfusion testing

Jack Hoffer, William P. Koslosky, Elizabeth S. Gloster, Therese M. Dimaio, Marion E. Reid

Immunohematology, Volume 15 , ISSUE 3, 105–107

Article | 14-October-2020

Variations in pretransfusion practices

Beverly J. Padget, Judith L. Hannon

Immunohematology, Volume 19 , ISSUE 1, 1–6

Article | 18-October-2020

Comparison of tube and gel techniques for antibody identification

Marcia Cristina Zago Novaretti, Eduardo Jens Silveira, Edio da Costa Filho, Pedro Enrique Dorlhiac- Llacer, Dalton de Alencar Fischer Chamone

Immunohematology, Volume 16 , ISSUE 4, 138–141

Report | 24-March-2020

Automation in the transfusion service

Suzanne H. Butch

Immunohematology, Volume 24 , ISSUE 3, 86–92

Article | 09-November-2020

The gel test: sensitivity and specificity for unexpected antibodies to blood group antigens

W. John Judd, E. Ann Steiner, Pamela C. Knaf

Immunohematology, Volume 13 , ISSUE 4, 132–135

Article | 06-December-2020

Evaluation of patients with positive direct antiglobulin tests and nonreactive eluates discovered during pretransfusion testing

Jerald A. Clark, Patricia C. Tanley, Charles H. Wallas

Immunohematology, Volume 8 , ISSUE 1, 9–12

Article | 16-October-2019

Warm autoadsorption using ZZAP

The masking of clinically significant alloantibodies by warm autoantibodies presents challenges in pretransfusion testing. The adoption of transfusion practices such as the issuing of “least incompatible” red blood cells (RBCs) without a complete antibody workup is potentially unsafe for patients. Several autoadsorption methods can be used to remove autoantibody reactivity. ZZAP treatment of autologous RBCs is an efficient way to prepare the cells for autoadsorption. Autoadsorbed

Farai M. Tsimba-Chitsva, Amy Caballero, Becky Svatora

Immunohematology, Volume 34 , ISSUE 1, 1–3

Article | 15-April-2020

Chimerism and mosaicism are important causes of ABO phenotype and genotype discrepancies

Discrepancies between blood group genotype and RBC phenotype are important to recognize when implementing DNA-based blood grouping techniques. This report describes two such cases involving the ABO blood group in the Korean population. Propositus #1 was a 22-year-old healthy man undergoing pretransfusion testing for minor surgery. Propositus #2 was a 23year-old male blood donor. RBCs from both propositi were determined to be group AB and demonstrated unusual agglutination patterns on forward

Duck Cho, Jin Sol Lee, Mark Harris Yazer, Jong Won Song, Myung Geun Shin, Jong Hee Shin, Soon Pal Suh, Mee Jeong Jeon, Ji Young Kim, Jong Tae Park, Dong Wook Ryang

Immunohematology, Volume 22 , ISSUE 4, 183–187

Article | 16-October-2019

Use of the prewarm method for detecting clinically significant alloantibodies in the presence of cold autoantibodies

interfere with standard testing methods by reacting at the 37°C and antihuman globulin test phases. When using the PW method, it is important to identify underlying, potentially clinically significant alloantibodies during pretransfusion testing to ensure the most appropriate component will be selected for transfusion.

Stephanie Dupuis

Immunohematology, Volume 34 , ISSUE 4, 148–150

Case report | 29-December-2020

Evaluation of a complement-dependent anti-Jka by various sensitization and detection methodologies: a case report

. The anti-Jka was detected only when polyspecific AHG and anti-C3 were used compared to anti-IgG, regardless of the selected enhancement technique. Anti-C3 gave weaker reactions than polyspecific AHG. IgG subclassing was inconclusive. The anti-Jka was not detected when plasma was substituted for serum. The use of polyspecific versus IgG AHG in pretransfusion testing is discussed.

E. Nicole DeLong

Immunohematology, Volume 4 , ISSUE 3, 59–63

Article | 15-February-2021

Rh immune globulin: an interfering substance in compatibility testing

DAT is positive or negative, may detect anti-D for a few weeks after infusion of IV RhIG. Serologic Challenges The encounter of true alloimmunization due to D+ RBCs resulting in anti-D is generally considered an unusual event in today’s pretransfusion testing setting, especially with current practices in place in transfusion therapy and obstetrical services to prevent the development of the antibody. Nevertheless, the detection of anti-D often prompts questions and actions by the transfusion

T.S. Casina, S.G. Sandler, S.M. Autenrieth

Immunohematology, Volume 35 , ISSUE 2, 51–60

Article | 06-December-2020

Reactive lysis - a phenomenon of delayed hemolytic transfusion reactions

A 62-year-old female with Gaucher's disease demonstrated alloanti-c on pretransfusion testing. She was transfused with five units of c-negative red blood cells (RBCs) preoperatively and intraoperatively. The hemoglobin (Hb) level was slightly lower initially, but was markedly lower on day 10 posttransfusion. Serologic results indicated a delayed hemolytic transfusion reaction (DHTR) due to alloanti-s, -Fya, and -Jkb, present both on the RBCs and in the serum. As late as day 35

Deborah L. Greene, Sanobar Khan

Immunohematology, Volume 9 , ISSUE 3, 74–77

Case report | 20-March-2020

New serologic findings in a patient with ulcerative colitis and a warm autoantibody

of ulcerative colitis but no signs of hemolytic anemia. A case of IgG-RBC sensitization associated with serine proteases and a warm autoantibody in a 14-yearold Hispanic girl with ulcerative colitis is reported. The patient was admitted for severe anemia (Hb, 6.9 g/dL). On admission, pretransfusion testing of the patient’s serum and RBCs showed an ABO/Rh discrepancy between the forward typing and reverse grouping. The phenomenon of IgG-RBC sensitization associated with serine proteases was

Thomas G. Lightfoot, Laurie Delia VanThof

Immunohematology, Volume 25 , ISSUE 4, 160–164

Case report | 28-April-2020

Case report: immune anti-D stimulated by transfusion of fresh frozen plasma

D antigen is highly immunogenic and the level of RBC contamination of FFP is not always known. This case highlights that transfusion of FFP is a stimulus for RBC antibodies and that when a patient has had a recent transfusion of FFP,consideration should be given to obtaining a sample for pretransfusion testing within 3 days before a scheduled RBC transfusion. In addition,the D status of FFP should be considered before administering FFP to premenopausal D– women.

Marian Connolly, Wendy N. Erber, Dianne E. Grey

Immunohematology, Volume 21 , ISSUE 4, 149–151

Case report | 01-December-2019

Anti-Ge2: further evidence for lack of clinical significance

hemoglobin concentration of 6.3 g/dL. During pretransfusion testing, anti-Ge2 was identified in his serum. Only a single unit of compatible, Ge:–2 frozen red blood cells (RBCs) could be provided by the blood supplier. A second unit of crossmatched, least-incompatible, leukocyte-reduced RBCs, presumably Ge:2, was also transfused. The transfusion was completed without incident, and the patient’s hemoglobin concentration rose appropriately. Posttransfusion values for haptoglobin, lactate

Deepthi Karunasiri, Frederick Lowder, Nora Ostrzega, Dennis Goldfinger

Immunohematology, Volume 30 , ISSUE 4, 156–157

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