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  • Immunohematology


Report | 01-December-2019

Effects of pH changes of stock normal saline solution on 5 percent red cell suspension

The red cell suspension (RCS) is a universally used indicator system to demonstrate antigen and antibody reactions in vitro. Saline solutions that are used in its preparation are preferred to be fresh to avoid changes in pH that may affect the results. Thus, buffered saline such as phosphate-buffered saline (PBS) is the ideal diluent because its pH is maintained for a certain period. However, normal saline solution (NSS) is more commonly used because it is inexpensive and easy to make. pH

Gregorio L. Martin, Princess Joy M. Caraan, Jared Jenrik S. Chua, Jules Albert L. Crescini, Joseph Martin C. Diokno, Christopher Bernard Dlr. Javier, Kristina Barbara O. Reyes, Rianne Y. Soliven

Immunohematology, Volume 30 , ISSUE 3, 126–134

Article | 17-November-2020

ABO discrepancy with monoclonal ABO reagents caused by a pH-dependent autoantibody

ABO discrepancy was noted when a patient’s unwashed saline red cell suspension was tested with monoclonal ABO reagents. The discrepancy was resolved when a washed (x 3) saline red cell suspension was used in repeat testing. The patient’s serum contained an autoagglutinin that reacted optimally below pH 7.0. The discrepancy occurred when monoclonal ABO reagents, formulated at a low pH, lowered the pH of the reaction mixture, and autoagglutination was observed.

Melanie S. Kennedy, Abdul Waheed, Jane Moore

Immunohematology, Volume 11 , ISSUE 3, 71–73

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