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review-article | 18-June-2021

Ultrasound of the neonatal hip as a screening tool for DDH: how to screen and differences in screening programs between European countries

acetabulum stimulates normal spherical development and central position of the femoral head, and vice versa. Ultrasound of the neonatal hip plays a role in screening, diagnosis, and (monitoring of) treatment of DDH. This article reviews the epidemiology, static and dynamic ultrasound techniques in screening for DDH, and differences in screening programs throughout Europe. Developmental dysplasia of the hip The incidence of DDH varies from 1.5 to 20 per 1,000 births, depending on the criteria used for

Iris Kilsdonk, Melinda Witbreuk, Henk-Jan Van Der Woude

Journal of Ultrasonography, Volume 21 , ISSUE 85, e147–e153

Report | 11-March-2020

Should we be screening for anti-Jsa?

We analyzed our historic patient database at North Shore University Hospital and determined both the overall frequency of anti-Jsa and the frequency at which it was detected in combination with other alloantibodies to red blood cell (RBC) antigens. Screening cells used currently are negative for Jsa. Our data suggest that anti-Jsa would not be detected in 30 to 40 percent of patients in which it is the sole antibody present. Since 1996 the antibody was only detected when other antibodies were

Nancy M. Nikolis, Fouad Boctor, William Andrew Heaton, James Martone

Immunohematology, Volume 27 , ISSUE 3, 104–106

Report | 01-December-2019

Single-center comparison of gel microcolumn and solid-phase methods for antibody screening

Our facility changed antibody screening methods from a gel microcolumn–based test (ID-Micro Typing System Gel Test; Ortho Clinical Diagnostics, Inc., Raritan, NJ) to an automated solid-phase test (Galileo/Capture-R Ready-Screen [I and II], Immucor, Inc., Norcross, GA). To determine whether detection rates for commonly encountered clinically significant red blood cell antibodies differed as a consequence of this change, preimplementation and postimplementation antibody identification

Anne Schmidt, Brenda J. Bendix, Eapen K. Jacob, Sandra C. Bryant, James R. Stubbs

Immunohematology, Volume 29 , ISSUE 3, 101–104

Research Article | 29-December-2017

The importance of community screening of asymptomatic elderly for peripheral arterial disease by Doppler ultrasound and ankle-brachial index

BackgroundPeripheral arterial disease is an atherosclerotic disease characterized by an increase in morbidity and mortality. For these reasons early diagnosis of peripheral arterial disease is important. Ankle-brachial systolic pressure index measurement is frequently used in screening studies. Evaluating waveforms of distal lower extremities with Doppler ultrasound can be used as a screening program and provides more accurate information on peripheral arterial disease.AimWe investigate the

Ayşe Keven, Mehmet Sedat Durmaz

Journal of Ultrasonography, Volume 17 , ISSUE 71, 235–240

Article | 26-October-2020

Provision of HPA-1a (PlA1)-negative platelets for neonatal alloimmune thrombocytopenia: screening, testing, and transfusion protocol

HPA-1a-negative platelet products are not routinely available for newborns with alloimmune thrombocytopenia. In this article we describe a program established to identify normal pheresis donors who are HPA-1a-negative and to organize their future donations so that our regional blood center would always have an HPA-1a-negative platelet product available. The solid phase red cell adherence assay was used for initial screening of platelet pheresis products. HPA-1a-negative donors were confirmed

Mary Munizza, Sandra Nance, Maryann Keashen-Schnell, William Sherwood, Scott Murphy

Immunohematology, Volume 15 , ISSUE 2, 71–74

original-paper | 12-May-2020

PCR-based Screening Approach: A Rapid Method to Detect the Biosynthetic Potential of Antimicrobials in Actinobacterial Strains

Abbreviations CYP– cytochrome P450 hydroxylase NRPS– non-ribosomal peptide synthase PKS-1– polyketide synthase Introduction The gene-based screening allows the rapid detection of biosynthetic gene clusters in the isolated strains (Wood et al. 2007). In the latest years, genome mining has been focused on Streptomyces and has become a novel and rapid method to identify the previously unidentified gene clusters (Xu et al. 2019). Genes that are involved in the biosynthesis of secondary

NAILA NOUREEN, MOHSIN TASSAWAR CHEEMA, SUMAIRA ANWAR, SHAHIDA HASNAIN, IMRAN SAJID

Polish Journal of Microbiology, Volume 69 , ISSUE 2, 139–149

research-article | 21-July-2020

ISOLATION AND SCREENING OF BACTERIA WITH ABILITY TO DECOLORIZE SELECTED SYNTHETIC DYES – PRELIMINARY RESULTS

multiplication of adapted microflora for the purpose to creating biopreparation for bioaugmentation of treatment systems may be an interesting solution [4, 16, 18, 23, 24, 25, 26, 27, 28]. The most popular sources of obtaining decolorizing bacteria are industrial sewages, especially textile wastewater [8, 17, 24]. The aim of the study was to show if other source materials may be used for izolation the bacteria with high abilities of decolorization of synthetic dyes. The screening was led for decolorization

Ewa ZABŁOCKA-GODLEWSKA, Wioletta PRZYSTAŚ, Maria GODLEWSKA

Architecture, Civil Engineering, Environment, Volume 13 , ISSUE 2, 75–86

Article | 14-October-2020

Antibody screening in 37°C saline. Is it safe to omit it using the indirect antiglobulin (gel) test?

pretransfusion samples were tested as they were received (from May 1998 to December 1999), in 37°C saline and by IAT using the DiaMed gel system. The screening tests were performed using 50 µL of 0.8% low-ionic-strength saline suspended RBCs and 50 µL of plasma. The tests were examined for agglutination and hemolysis. Two hundred and thirty three samples (9.81%) were reactive by IAT and 88 (3.70%) by 37°C saline. All 88 samples reactive by 37°C saline also reacted by IAT. These data

José A. Duran, Manuel Figueiredo

Immunohematology, Volume 18 , ISSUE 1, 13–15

Article | 17-February-2021

A prospective, observational study for optimization of antibody screening in pretransfusion compatibility testing

Antibody screening (AS) is an important component of a pretransfusion immunohematology workup. Type and screen (TS) followed by an immediate spin test (IST) crossmatch is considered superior to type and hold followed by an antihuman globulin (AHG) crossmatch. To make blood transfusions as safe as possible, it is desirable to perform AS for patients using a reagent red blood cell (RBC) panel called an antibody screening panel, which consists of two or more phenotyped RBC reagents. If a

P. Pandey, D. Setya, R. Srivastava, M.K. Singh

Immunohematology, Volume 36 , ISSUE 1, 19–28

Original Paper | 30-June-2018

Screening and Identification of Trichoderma Strains Isolated from Natural Habitats with Potential to Cellulose and Xylan Degrading Enzymes Production

A total of 123 Trichoderma strains were isolated from different habitats and tested for their ability to degrade cellulose and xylan by simple plate screening method. Among strains, more than 34 and 45% respectively, exhibited higher cellulolytic and xylanolytic activity, compared to the reference strain T. reesei QM 9414. For strains efficiently degrading cellulose, a highest enzyme activity was confirmed using filter paper test, and it resulted in a range from 1.01 to 7.15 FPU/ml. Based on

ROMAN MARECIK, LIDIA BŁASZCZYK, RÓŻA BIEGAŃSKA-MARECIK, AGNIESZKA PIOTROWSKA-CYPLIK

Polish Journal of Microbiology, Volume 67 , ISSUE 2, –

Article | 30-July-2021

Orthodontic screening and referral practices of dental therapists in New Zealand

Background: Timely referrals of appropriately screened cases are essential for the effective delivery of orthodontic treatment. Dental therapists are intimately involved with the orthodontic screening process in New Zealand, given that they are the primary oral health providers for child and adolescent patients. Objectives: (1) To investigate New Zealand dental therapists’ orthodontic screening and referral practices; and, (2) to quantify the perceived need for supplementary orthodontic

Nadarren Lim, Florence Bennani, Li Mei, William Murray Thomson, Mauro Farella, Joseph Safwat Antoun

Australasian Orthodontic Journal, Volume 33 , ISSUE 2, 158–169

Review Paper | 07-June-2016

Depression and anxiety in people with epilepsy: Why should we identify?

validity of simple screening tools for the detection of depression and anxiety. Review. Approximately one quarter of PWE have been known to be suffered from depression. The frequency of depression and anxiety was closely related to poor seizure control. Depression and anxiety have been reported to have a bidirectional relationship with epilepsy. The higher degree of depression and anxiety was more likely to elicit the suicidal ideation and attempt, adverse events and poor compliance of antiepileptic

Sung-Pa Park

Journal of Epileptology, Volume 24 , ISSUE 1, 57–62

research-article | 30-November-2020

Development and initial validation of the Odense Child Trauma Screening: a story stem screening tool for preschool and young schoolchildren

the internal world of young children in an age-appropriate and sensitive manner with the child as informant (10). This paper examines reliability and validity of a new, standardized story stem assessment tool, the Odense Child Trauma Screening (OCTS; 11), that was developed by the authors to screen for behavioural and play-based indicators of traumatization in children aged 4 to 8 years. Story stem assessment tools The technique of a narrative story stem assessment tool involves the child being

Sille Schandorph Løkkegaard, Mette Elmose, Ask Elklit

Scandinavian Journal of Child and Adolescent Psychiatry and Psychology, Volume 9 , 113–126

Article | 14-October-2020

MIMA-9, a valuable antibody for screening for rare donors

Since monoclonal antibodies (Mabs) are potentially available in an unlimited volume, they can be used to screen numerous donor blood samples to identify antigen-negative donors. We have used a Mab (MIMA-9) with characteristics that allow for the simultaneous screening of RBCs of any ABO group for high-incidence antigennegativity in the Kell and Gerbich blood group systems. MIMA-9, a murine IgG2a antibody, previously shown to facilitate the identification of K+k–, Kp(a+b–), K0

Edith Tossas, Ragnhild Øyen, Gregory R. Halverson, Harry Malyska, Marion E. Reid

Immunohematology, Volume 18 , ISSUE 2, 43–45

Article | 30-July-2021

A qualitative study of orthodontic screening and referral practices among dental therapists in New Zealand

Background: Appropriate screening and referral is important in delivering effective and timely orthodontic treatment. In New Zealand, dental therapists are usually the initial contact for oral health care (including the assessment of malocclusion) in child and adolescent patients. Objective: To investigate the orthodontic screening and referral practices of dental therapists in New Zealand. Methods: Ten registered dental therapists from Auckland and Dunedin were interviewed using a semi

Allan Tan, Florence Bennani, W. Murray Thomson, Mauro Farella, Li Mei

Australasian Orthodontic Journal, Volume 32 , ISSUE 2, 155–164

Article | 14-October-2020

Significance of platelet-reactive antibody screening for patients facing frequent platelet transfusions

It is not clear whether platelet-reactive antibody screening is clinically significant for patients facing frequent platelet transfusions. On the basis of data from 96 patients who had been examined for platelet-reactive antibodies by the mixed passive hemagglutination method for a variety of reasons, we investigated the following three issues retrospectively: (1) the relationship between platelet-reactive antibodies and the occurrence of problems in platelet transfusions, such as

Tetsunori Tasaki, Kieko Fujii, Kenji Gotoh, Shyukuko Satoh, Jyunko Takadate, Sakiko Sasaki, Mihoko Tachibana, Kimiko Yamamoto

Immunohematology, Volume 18 , ISSUE 4, 104–108

Report | 25-March-2020

Value of DNA-based  assays for donor screening and  regulatory issues

Hemagglutination, the gold standard method to detect the presence or absence of blood group antigens on RBCs, has served the transfusion community well for decades.  It is simple, and, when done correctly, it has a specificity and sensitivity that is appropriate for most testing in the vast majority of patients requiring blood transfusion.  The limitations of hemagglutination for screening donor blood include that both testing and data entry are labor-intensive, that the required

Donna Strauss, Marion E. Reid

Immunohematology, Volume 24 , ISSUE 4, 175–179

Article | 14-October-2020

Equivalence of spray-dried K2EDTA,spray-dried K3EDTA, and liquid K3EDTA anticoagulated blood samples for routine blood center or transfusion service testing

We compared the results of routine blood tests for 102 blood donors’samples and 100 patients’samples collected in spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes to evaluate the impact of changes in formulation of the anticoagulant (K2EDTA vs.K3EDTA), its application (liquid vs.spraydried), and tube material (glass vs. plastic). Methods for ABO/D testing, antibody screening, and antibody identification included direct hemagglutination/microplate

Stacie Leathem, Nicole Dodge Zantek, Marti Kemper, Laura Korte, Al Langeberg, S. Gerald Sandler

Immunohematology, Volume 19 , ISSUE 4, 117–121

Article | 06-December-2020

Cytomegalovirus antibody screening on the Olympus PK7100

of 99.9 percent and 100 percent respectively, for the Olympus PK™ CMV system. Forty percent of 1,807 random blood donors in this region had antibodies to CMV. CMV testing can be performed simultaneously with blood grouping and red cell antibody screening on the Olympus PK7100.

LioneI A. Mohabir

Immunohematology, Volume 8 , ISSUE 2, 41–43

research-article | 09-January-2020

The Use of Travel Time to Measure Geographic Accessibility to Breast Screening Services in New South Wales, Australia Short Paper

the proportion of breast cancers identified at an early stage, allowing for more effective treatment to be used and reducing the risks of death from breast cancer. Research by Roder et al. (2008) found that participation in screening was associated with a breast cancer mortality reduction of between 30 and 41%, depending on assumptions about screening self-selection bias. The BreastScreen Australia programme was introduced in 1991 and is funded and coordinated jointly through federal, state, and

Deborah van Gaans, Neil Coffee, Theo Niyonsenga, Catherine Miles, Matthew Warner-Smith, Mark Daniel, David Roder, Daniel J. Weiss

South Australian Geographical Journal, Volume 115 , ISSUE 1, 1–7

Report | 01-December-2019

Performance characteristics of two automated solid-phase red cell adherence systems for pretransfusion antibody screening:  a cautionary tale

Our institution has implemented two instruments, the Galileo and the Echo, that use different solid-phase red cell adherence assays for antibody screening in pretransfusion compatibility testing. During the initial implementation of these two instruments, we noticed very different problems: falsely positive results on the Galileo, and falsely negative results and lack of reproducibility on the Echo. Comparison of falsely positive antibody screen results from approximately equivalent numbers of

Karen Quillen, James Caron, Kate Murphy

Immunohematology, Volume 28 , ISSUE 4, 137–139

Research Article | 18-October-2018

Validity of the Brief Child and Family Phone Interview by comparison with Longitudinal Expert All Data diagnoses in outpatients

, oppositional defiant disorder, conduct disorder, separation anxiety disorder, generalized anxiety disorder, and major depressive disorder.Results:The criterion validity for BCFPI versus LEAD diagnoses was fair for oppositional defiant disorder (area under curve, 0.73), generalized anxiety disorder (0.73) and major depressive disorder (0.78), good for attention-deficit hyperactivity disorder (0.81) and conduct disorder (0.83), and excellent for separation anxiety disorder (0.90). The screening properties of

Markus Andersson, Martin Bäckström, Tord Ivarsson, Maria Råstam, Håkan Jarbin

Scandinavian Journal of Child and Adolescent Psychiatry and Psychology, Volume 6 , ISSUE 2, 83–90

Article | 15-April-2020

In search of the Holy Grail: comparison of antibody screening methods

Tony S. Casina

Immunohematology, Volume 22 , ISSUE 4, 196–202

Report | 01-December-2019

Should blood donors be routinely screened for irregular antibodies?

components to avoid transfusion-related acute lung syndrome. Total blood donor population between 2007 and 2009 was 60,309 (55.4% male and 44.6% female). Cells I and II were used for alloantibody screening following the Autovue protocol. Positive samples were identified by red blood cell (RBC) panels (Panel A, Panel B, and Panel C, Ortho Clinical Diagnostics, Raritan, NJ). Alloantibody and CSAA frequency were established for both sexes. The database for RBC antigens estimated for the Colombian population

Michel Andrés García, Leonardo Bautista, Fernando Palomino

Immunohematology, Volume 28 , ISSUE 2, 60–66

Article | 14-October-2020

Antibodies detected in samples from 21,730 pregnant women

Although anti-D is still the main cause of HDN, many other antibodies have been implicated. From September 1995 to April 2000,screening for RBC antibodies was performed on samples from 21,730 pregnant women regardless of RhD type. Standard tube and gel methods were used. Anti-D was identified in 254 samples;other antibody specificities were detected in 376 samples, for a total of 630 antibodies. For this study, 522 antibodies were considered clinically significant. The incidence of potentially

Snezana Jovanovic-Srzentic, Milan Djokic, Nenad Tijanic, Radmila Djordjevic, Nada Rizvan, Darko Plecas, Dejan Filimonovic

Immunohematology, Volume 19 , ISSUE 3, 89–92

research-article | 30-November-2018

Cell-based immunofluorescence assay for screening the neurogenesis potential of new drugs in adult hippocampal neural progenitor cells

be used to detect neurogenesis, including in vivo and in vitro labeling with the thymidine analogue, bromodeoxyuridine (BrdU), endogenous cell-cycle markers, and cell stage and lineage commitment markers (Kuhn et al., 2016). Each method has its own strengths and limitations; however, when several compounds require quick screening to determine whether they promote neurogenesis, these methods may be unsuitable as they require several reagents and are time-consuming. Therefore, we propose a cell

Kun Zhang, Bin Li, Peifang Li, Xiaoli Yang, Huixian Cui, Xiaoyun Liu

Acta Neurobiologiae Experimentalis, Volume 79 , ISSUE 3, 303–309

Research Article | 31-May-2018

Identification of HG Types of Soybean Cyst Nematode Heterodera glycines and Resistance Screening on Soybean Genotypes in Northeast China

Cui Hua, Chunjie Li, Yanfeng Hu, Yanzhi Mao, Jia You, Mingze Wang, Jingsheng Chen, Zhongyan Tian, Congli Wang

Journal of Nematology, Volume 50 , ISSUE 1, 41–50

Article | 14-October-2020

Variations in pretransfusion practices

autocontrol, and DAT, and immediate spin and 37°C microscopic readings. Nine percent never perform an Rh control with anti-D typing on patient samples. Various antibody screening and crossmatch methods are utilized. Individual laboratory test practices should be periodically assessed to ensure that they comply with standards, represent the recognized best practice, and are cost-effective. The survey responses indicate that many laboratories perform tests that are not necessary or cost-effective. These

Beverly J. Padget, Judith L. Hannon

Immunohematology, Volume 19 , ISSUE 1, 1–6

Article | 22-January-2021

Routine indirect antiglobulin testing of blood donors—a further step toward blood safety: an experience from a tertiary care center in northern India

transfusions, since the presence of unexpected antibodies in the donor plasma can lead to adverse transfusion reactions, particularly in cases in which a large amount of plasma is transfused (as in massive transfusions) and in pediatric patients.3 In this scientific article, we emphasize the importance of establishing a policy for antibody screening of all blood donors as a step to further improve blood safety. The policy of routine inclusion of the indirect antiglobulin test (IAT) was adopted in our

S. Malhotra, G. Negi, D. Kaur, S.K. Meinia, A.K. Tiwari, S. Mitra

Immunohematology, Volume 36 , ISSUE 3, 93–98

research-article | 30-November-2020

Evaluation of root-lesion nematode (Pratylenchus zeae) resistance assays for sugarcane accession lines

resistance in the progenies derived from crosses between Erianthus spp. and Saccharum spontaneum lines with commercial sugarcane. The first component of that work was to develop methods for screening large numbers of sugarcane lines against root-lesion nematodes. Initial research by the authors determined trial conditions, suitable potting media, and extraction methods for root-lesion nematodes (Bhuiyan et al., 2014). The other important aspect was to formulate an assessment method for nematode

S. A. Bhuiyan, K. Garlick

Journal of Nematology, Volume 53 , 1–10

Article | 17-November-2020

Flow cytometric phenotyping of platelet HPA-1a antigen: donor screening for a case of neonatal alloimmune thrombocytopenia due to anti-HPA-1a antibodies

screening. Using this method, several HPA-1anegative donors were identified, and one of them donated platelets that were successfully transfused to the thrombocytopenic newborn.

Johannes J.M.L. Hoffmann, Willy C.M. Janssen, Harry Y. von Hegedus

Immunohematology, Volume 11 , ISSUE 4, 125–128

research-article | 30-November-2020

Evaluation of root-knot nematode resistance assays for sugarcane accession lines in Australia

nematodes (Stirling et al., 2011) and were tolerant to water logging and drought (Foreman et al., 2007). A research project was commenced by Sugar Research Australia (SRA, formerly BSES) in 2011 to determine the level of nematode resistance in germplasm from domesticated and wild species and in the progenies derived from crosses between sugarcane and both Erianthus spp. and S. spontaneum lines with commercial sugarcane. The first component of that work was to develop methods for screening large numbers

S. A. Bhuiyan, K. Garlick

Journal of Nematology, Volume 53 , 1–11

Article | 03-November-2020

Rapid screening of platelet donors for PlA1 (HPA-1a) alloantigen using a solid-phase microplate immunoassay

indicated the platelets were PlA1-negative. Of 520 donors, 15 (2.88%) tested PlA1-negative, which correlates well with the reported PlA2,2 frequency in whites of 2.25 percent. Results were confirmed by DNA genotyping and/or immunoblotting. This screening technique permits phenotyping donors for PlA1 alloantigen with minimal specialized equipment. Confirmatory testing for PlA2 alloantigen can be reserved for donors that test negative for PlA1.

Jo L. Procter, Faye E. Vique, Ed Alegre, Junichi Honda, Kazuhiko Matsuo, Diane Reid

Immunohematology, Volume 14 , ISSUE 4, 141–145

Report | 14-March-2020

Comparison of gel test and conventional tube test for antibody detection and titration in D-negative pregnant women: study from a tertiary-care hospital in North India

Conventional tube testing was used for antibody screening and titration in D– pregnant women in our hospital until the recent introduction of the gel test. In this study we assessed the sensitivity of the gel test in our setup and tried to establish a correlation between these tests for determining antibody titer. We collected 652 blood samples from 223 antenatal D– women during a span of 1 year. The samples were tested separately by the conventional tube technique and the gel test

Manish K. Thakur, Neelam Marwaha, Praveen Kumar, Subhash C. Saha, Beenu Thakral, Ratti Ram Sharma, Karan Saluja, Hari Krishan Dhawan, Ashish Jain

Immunohematology, Volume 26 , ISSUE 4, 174–177

Report | 20-March-2020

A simple screening assay for the most common JK*0 alleles revealed compound heterozygosity in Jk(a–b–) probands from Guam

not routinely available. Identification of Jk(a–b–) patients and donors is most often performed serologically. However, ten JK*0 alleles have been identified, and this information can be used in DNA-based typing. We selected five JK*0 alleles that had been encountered by our reference laboratory in two or more samples from unrelated individuals and designed an allele-specific primer PCR assay for use as an initial screening tool. After in-house validation, we tested genomic DNA from a

Elisabet Sjöberg Wester, Julia Gustafsson, Beverly Snell, Peggy Spruell, Åsa Hellberg, Martin L. Olsson, Jill R. Storry

Immunohematology, Volume 25 , ISSUE 4, 165–169

Review | 31-August-2018

Ultrasound screening for pyelectasis in pregnant women. Clinical necessity or “art for art’s sake”?

Biology classification. The pyelectasis volume measuring method using three-dimensional ultrasound scanning included in the criteria for the assessment of asymptomatic bacteriuria was assessed as sensitive and specific. The ultrasound-based evaluation of the kidneys for the presence of pyelectasis and its grade in pregnant women has some clinical implications. It allows for identifying cases with an increased risk of asymptomatic bacteriuria, which requires treatment in pregnant women. Screening

Piotr Szkodziak

Journal of Ultrasonography, Volume 18 , ISSUE 73, 152–157

Research paper | 31-October-2017

MULTINOMIAL LOGISTIC REGRESSION APPROACH FOR THE EVALUATION OF BINARY DIAGNOSTIC TEST IN MEDICAL RESEARCH

Evaluating the effect of variables on diagnostic measures (sensitivity, specificity, positive, and negative predictive values) is often of interest to clinical researchers. Logistic regression (LR) models can be used to predict diagnostic measures of a screening test. A marginal model framework using generalized estimating equation (GEE) with logit/log link can be used to compare the diagnostic measures between two or more screening tests. These individual modeling approaches to each diagnostic

Alok Kumar Dwivedi, Indika Mallawaarachchi, Juan B. Figueroa-Casas, Angel M. Morales, Patrick Tarwater

Statistics in Transition New Series, Volume 16 , ISSUE 2, 203–222

Article | 18-October-2020

Human anti-Dia monoclonal antibodies for mass screening

Toru Miyazaki, Shinichiro Sato, Toshiaki Kato, Hisami Ikeda

Immunohematology, Volume 16 , ISSUE 2, 78–81

Article | 17-February-2021

Severe hemolytic disease of the fetus and newborn due to anti-E and anti-Jka

Red blood cell (RBC) alloimmunization to antigens other than D, such as C, c, E, e, and antigens in the Kell, MNS, and Duffy blood group systems, has emerged as an important cause of hemolytic disease of the fetus and newborn (HDFN).1 Antibody screening for these antibodies is not routinely practiced for all antenatal patients in developing countries, mainly because of financial constraints. We report a case of HDFN in a female baby due to maternal alloimmunization against Rh and Kidd blood

S. Mandal, S. Malhotra, G. Negi, A. Tiwari, S. Mitra, S. Basu, P. Singh

Immunohematology, Volume 36 , ISSUE 2, 60–63

Article | 14-October-2020

Nondetection of the S antigen due to the presence of sodium hypochlorite

Low concentrations of sodium hypochlorite (chlorine bleach) are known to destroy S antigen on intact fresh red blood cells (RBCs). Sodium hypochlorite is commonly used as a disinfectant. We report nondetection of the S antigen in tube and microplate saline indirect antiglobulin testing (SIAT) with a lot of commercial saline utilized in our donor screening and reference laboratories. Known S+s+ RBCs were found to be nonreactive with anti-S by SIAT in our reference laboratory. Our investigation

Anne Long, Lyne Tremblay, Lucie Richard, Réal Lemieux, Mindy Goldman

Immunohematology, Volume 18 , ISSUE 4, 120–122

Letter to Editor | 06-December-2020

Letter to the Editor: Donor Screening With Anti-AnWj

Trish Lukasavage

Immunohematology, Volume 9 , ISSUE 4, 112–112

Commentary | 09-November-2020

Commentary: testing for unexpected red cell antibodies - two or three reagent red cell samples?

W. John Judd

Immunohematology, Volume 13 , ISSUE 3, 90–92

Article | 10-April-2021

A fatal case of acute hemolytic transfusion reaction caused by anti-Wra: case report and review of the literature

Wra is the most common low-prevalence antigen (LPA) in the white population, and anti-Wra is the most common naturally occurring antibody.1 The first case of anti-Wra was described by Holman2 in 1953 in a child with severe hemolytic disease of the fetus and newborn (HDFN), requiring exchange transfusion. Anti-Wra is often identified when Wr(a+) red blood cells (RBCs) are available on the screening or identification panel RBCs, but the antibody is otherwise rarely involved in serious hemolytic

A. Espinosa, L.J. Garvik, N. Trung Nguyen, B. Jacobsen

Immunohematology, Volume 37 , ISSUE 1, 20–24

research-article | 17-March-2020

Silver nanoparticles as a potential nematicide against Meloidogyne graminicola

AgNP for 100% mortality was identified as 0.1 µg/ml by 12 hr. The J2 did not recover after rinsing with water. Trisodium citrate alone had no effect on nematode immobility/mortality; therefore, the nematode mortality is attributed to AgNP exclusively. Direct exposure of AgNP to M. graminicola in sand Unlike water screening, 0.1 µg/ml of AgNP proved ineffective even up to 72 hr (Table 1). Juvenile mortality started at 0.2 µg/ml, and it increased with increasing concentration of AgNP and time (p

Richa Baronia, Puneet Kumar, S. P. Singh, R. K. Walia

Journal of Nematology, Volume 52 , 1–9

Article | 17-February-2021

Two Thai Burmese descendants with A4GALT*01N.21, p phenotype, and anti-PP1Pk

patient attended the antenatal clinic of the hospital. Thereafter, the patient was admitted at 24 weeks’ gestation because of spontaneous abortion. Although she did not require a blood transfusion, a sample was sent for investigation. Initial serologic results indicated both patients to be group B, D+ with positive antibody detection tests (partial hemolysis at 37°C). The antibody detection test was positive with all screening cells (O1 and O2, National Blood Centre, Thai Red Cross Society, NBC-TRC

K. Intharanut, W. Sasikarn, W. Chusri, O. Nathalang

Immunohematology, Volume 36 , ISSUE 2, 64–68

Article | 14-October-2020

DNA analysis for donor screening of Dombrock blood group antigens

Jill R. Storry, Connie M. Westhoff, Dalisay Charles-Pierre, Maria Rios, Kim Hue-Roye, Sunitha Vege, Sandra Nance, Marion E. Reid

Immunohematology, Volume 19 , ISSUE 3, 73–76

Article | 20-December-2020

Tech tip: procedure for rapid antigen screening with LIM reagent

Irene E. Stocker

Immunohematology, Volume 6 , ISSUE 4, 97–98

Letter to Editor | 03-November-2020

Letters to the Editors: Yes, screening based on ethnicity is worthwhile

Judy Martin, Wendy Kluver, A.J. Hibbard

Immunohematology, Volume 14 , ISSUE 3, 125–125

Case report | 26-October-2019

Blocked D phenomenon and relevance of maternal  serologic testing

Ashish Jain, Vijay Kumawat, Neelam Marwaha

Immunohematology, Volume 31 , ISSUE 3, 116–118

Article | 14-October-2020

Screening for RBC antibodies - what should we expect from antibody detection RBCs

George Garratty

Immunohematology, Volume 18 , ISSUE 3, 71–77

Article | 01-April-2020

External quality assessment scheme in red blood cell serology: a 5-year experience in Thailand

From 2000 to 2004, 36, 58, 72, 78,and 86 laboratories participated in an external quality assessment scheme (EQAS) organized by the Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital. Each year the staff was requested to perform ABO grouping,D typing,antibody screening,antibody identification,and DATs on eight blood samples. Each participant received information on the correct test results and a coded summary. Regarding ABO grouping, the error .rate ranged from 0.3 to 1.3

Sasitorn Bejrachandra, Jariya Saipin, Oytip Nathalang, Usanee Siriboonrit, Ekaraj Rungroung, Sudjai Udee

Immunohematology, Volume 22 , ISSUE 1, 1–5

Article | 17-November-2020

The r' gene is overrepresented in hrB-negative individuals

A screening program was implemented to identify hrB-donors. D-C+, D-C-, and D+C- samples from African-American donors were typed with multiple examples of anti-hrB and and-hrB-like, and one example each of anti-V and anti-VS. Of 75 D-C+ donors, 4 (5%) typed as hrB-, and 14 others had weak or variable expression of hrB. Of these 18 individuals, 15 were V-VS+, and 3 were V+VS+. No hrB- sample was found in 90 C- donors, 26 of whom were V+VS+, and 1 was V-VS+. A review of our records of 44 hrB

Connie L. Beal, Delores Mallory, Cindy Oliver, Peter D. Issitt

Immunohematology, Volume 11 , ISSUE 3, 74–77

Article | 17-November-2020

A comparison of two solid phase systems for antibody detection

Two solid phase methods of antibody detection, Capture-R (CR) and Capture-R Ready-Screen (RS), were compared to determine their acceptability for use in prenatal antibody screening. Ninety-six serum samples, screened using a saline antiglobulin test, were coded and tested by CR and RS at two laboratory sites using a blinded study design. Thirty of the samples were free of antibody, and 66 samples contained antibody. Parallel testing was also performed in both laboratories on 648 prenatal

Gwen M. Haslam, Margaret Persaud, Yvette Fournier, Joanne Sajur, Janice Aulph, Nancy Heddle

Immunohematology, Volume 11 , ISSUE 1, 8–10

Original Paper | 10-December-2018

Comparison of PCR, Fluorescent in Situ Hybridization and Blood Cultures for Detection of Bacteremia in Children and Adolescents During Antibiotic Therapy

Abstract The gold standard in microbiological diagnostics of bacteremia is a blood culture in automated systems. This method may take several days and has low sensitivity. New screening methods that could quickly reveal the presence of bacteria would be extremely useful. The objective of this study was to estimate the effectiveness of these methods with respect to blood cultures in the context of antibiotic therapy. Blood samples from 92 children with sepsis were analyzed. Blood cultures were

TOMASZ W. ŹRÓDŁOWSKI, DANUTA JURKIEWICZ-BADACZ, AGNIESZKA SROKA-OLEKSIAK, DOMINIKA SALAMON, MAŁGORZATA BULANDA, TOMASZ GOSIEWSKI

Polish Journal of Microbiology, Volume 67 , ISSUE 4, 479–486

Short Report | 10-September-2020

Can pregnant women accurately report snoring? A pilot study

Sleep-Disordered Breathing (SDB) has been associated with possible negative outcomes, such as preeclampsia and foetal growth restriction. SDB screening tools have been developed for use within general populations. These included the use of self-reports and objective measurements. Seventeen pregnant women within their 34th to 37th week of pregnancy were recruited.  Participants undertook an overnight study within their home and SDB symptoms were monitored using the Watch-PAT 200 and an

Jane Warland, Jacinta Carpentieri, Jillian Dorrian

Eat, Sleep, Work, Volume 1 , ISSUE 1, 57–64

Report | 26-October-2019

A simple approach to screen rare donors in Brazil

Providing blood units for patients with an antibody to a highprevalence antigen or with multiple common antibodies is a constant challenge to the blood banks. Finding a compatible donor requires extensive screening, which incurs a large amount of investment. In this article, we share our experience of organizing a rare donor inventory with limited resources, we include the strategy used for finding rare donors, and we share the difficulties found during the implementation of the approach and

Carine Prisco Arnoni, Flavia R.M. Latini, Janaína Guilhem Muniz, Rosangela Duarte de Medeiros Person, Tatiane Aparecida de Paula Vendrame, Diana Gazito, Lilian Castilho

Immunohematology, Volume 31 , ISSUE 1, 20–23

Article | 17-November-2020

A simple screening method to evaluate the presence of alloantibodies with concomitant warm autoantibodies

Ragnhild Øyen, Maria L. Angeles

Immunohematology, Volume 11 , ISSUE 3, 85–87

original-paper | 01-May-2021

Screening of Human Immunodeficiency Virus (HIV) among Newly Diagnosed Tuberculosis Patients in Eastern Sudan

GADA MUSTAFA AHMED MUSTAFA, MUSTAFA ELTIGANI YASSIN, ASHWAG SHAMI, SAMAH ABDU RAHIM

Polish Journal of Microbiology, Volume 70 , ISSUE 2, 201–206

Review | 26-October-2020

Hemochromatosis, iron, and blood donation: a short review

Angela C. Fields, Alfred J. Grindon

Immunohematology, Volume 15 , ISSUE 3, 108–112

Article | 10-November-2020

The impact of a gel system on routine work in a general hospital blood bank

The gel system has been reported to be more sensitive and specific than the conventional tube indirect antiglobulin test (IAT) for anti­body screening. However, a major concern about the gel system is its cost. A cost analysis study was therefore conducted at our hospi­tal. The gel system costs more than the conventional tube IAT per test; however, the total staff and reagent costs per year were about equal, because of staff savings. Workload and cost per patient requir­ing blood

A. Chan, H.F. Wong, C.H. Chui, L. Wong, G. Cheng

Immunohematology, Volume 12 , ISSUE 1, 30–32

Article | 06-December-2020

Antibody screening and direct antiglobulin testing using BioVueTM column agglutination technology versus standard tube tests

Susan F. South

Immunohematology, Volume 9 , ISSUE 3, 78–80

Research Article | 30-November-2014

The Early Diagnosis of Preschool Children with Autism Spectrum Disorder in Norway: a Study of Diagnostic Age and Its Associated Factors

The early identification of autism spectrum disorder is important to ensure access to early intervention. Much research has focused on the identification of early behavioral symptoms and screening. This study examines referral for diagnostic assessment, diagnostic age, and factors associated with diagnostic age for children with autism spectrum disorder. The results of this study indicate that children are referred and diagnosed later than they should be. Previous research has identified a

Kenneth Larsen

Scandinavian Journal of Child and Adolescent Psychiatry and Psychology, Volume 3 , ISSUE 2, 136–145

short-communication | 28-April-2020

Extensively Drug-resistant Acinetobacter baumannii Belonging to International Clone II from A Pet Cat with Urinary Tract Infection; The First Report from Pakistan

ZEESHAN TAJ, MUHAMMAD HIDAYAT RASOOL, AHMAD ALMATROUDI, MUHAMMAD SAQALEIN, MOHSIN KHURSHID

Polish Journal of Microbiology, Volume 69 , ISSUE 2, 231–234

Article | 20-December-2020

Red cell antibody identification by solid phase red cell adherence utilizing dried RBC monolayers

Recent technological advances in the immobilization and drying of red cell monolayers for use in solid phase red cell adherence (SPRCA) assays have resulted in the development of reagent red cells for antibody screening and identification that are stable at room temperature. Panels consisting of twelve different RBC samples dried onto individual microplate wells were evaluated with 176 samples whose antibody specificities had previously been determined by conventional hemagglutination

Darryl L. Stone, Ralph A. Eatz, Susan D. Rolih, Seaborn J. Farlow, Gordon S. Hudson, Lyle T. Sinor

Immunohematology, Volume 6 , ISSUE 1, 12–17

Article | 16-February-2021

Acidification of plasma for detection of pH-dependent antibodies

screening more than 1000 random NN donors, Kathryn M. Beattie and Wolf W. Zuelzer found an additional 23 examples of this pH-dependent anti-M. The data presented by Beattie and Zuelzer support the hypothesis that the acidification of the serum, and therefore the addition of hydrogen ions, activates or enhances the charge of the antibody molecule facilitating agglutination. Subsequent alkalization and a decrease in hydrogen ions result in the reduction of the antibody charge and the disassociation of the

K.L. Bowman, B.C. Dunlap, L.M. Hawthorne, K.L. Billingsley

Immunohematology, Volume 35 , ISSUE 3, 116–118

Case report | 26-October-2019

Suspected acute hemolytic transfusion reaction mediated by anti-Dia

antibody screening has been unremarkable, particularly when electronic crossmatch is used, because of the potential for an alloantibody against a lowprevalence antigen.

Ashwini Bennett, Ray K. Boyapati, Frank S. Hong

Immunohematology, Volume 31 , ISSUE 4, 163–165

Article | 08-April-2018

Analysis and Study the Correlation of Genetic Loci

Based on information of 1000 samples provided by single nucleotide polymorphisms, analyzing the existing SNP data, using the optimized linear mixed model for data screening of correlation analysis, the coding mode of each given locus bases (A, T, C, G) are encoded into numerical encoding, with correlation analysis of collection of evolutionary algorithms, the corresponding figure in Manhattan and risk point and the position of the gene are obtained for further operations of the transferred

Liu Yang1,, Bai Xiaojun2, Kang Xiaofeng3

International Journal of Advanced Network, Monitoring and Controls, Volume 1 , ISSUE 2, 118–124

Article | 16-October-2019

Low-ionic-strength saline solution–antiglobulin test (LISS-AGT)

The use of low-ionic-strength saline (LISS) solution as an enhancement for antibody screening and crossmatching was first described by Löw and Messeter in 1974. This method allowed for a reduced incubation time while maintaining adequate specificity and sensitivity of the antiglobulin test (AGT). Since then, the LISS-AGT tube method has been widely used in antibody detection and identification, as well as compatibility testing. As initially described, the method used red blood cells

LeeAnn Walker

Immunohematology, Volume 34 , ISSUE 2, 57–60

Research Article | 30-March-2018

Utility of Doppler parameters at 36–42 weeks’ gestation in the prediction of adverse perinatal outcomes in appropriate-for-gestational-age fetuses

Michał Migda, Katarzyna Gieryn, Bartosz Migda, Marian Stanisław Migda, Marek Maleńczyk

Journal of Ultrasonography, Volume 18 , ISSUE 72, 22–28

Original Paper | 30-March-2017

Screening and Identification of Yeasts Antagonistic to Pathogenic Fungi Show a Narrow Optimal pH Range for Antagonistic Activity

Pei-Hua Chen, Jui-Yu Chou

Polish Journal of Microbiology, Volume 66 , ISSUE 1, 101–106

Original Paper | 10-December-2018

Epilithic Biofilms in Lake Baikal: Screening and Diversity of PKS and NRPS Genes in the Genomes of Heterotrophic Bacteria

ELENA SUKHANOVA, EKATERINA ZIMENS, OKSANA KALUZHNAYA, VALENTINA PARFENOVA, OLGA BELYKH

Polish Journal of Microbiology, Volume 67 , ISSUE 4, 501–516

Article | 06-December-2020

En(a-) phenotype in a Japanese blood donor

The first Japanese En(a-) individual (T.N.) was found by screening red cells from 250,000 Japanese blood donors with monoclonal anti-Ena. His serum contained no atypical antibodies and his partial red cell phenotype was M-N-S+s-, although a trypsin-resistant N antigen was detected. His red cells were En(a-) and Wr(b-), as determined by various human and mouse monoclonal antibodies. The absence of glycophorin A (GPA) and the presence of apparently normal glycophorin B (GPB) were demonstrated by

Yasuto Okubo, Taiko Seno, Hideo Yamaguchi, Yoshihisa Miyata, Carole A. Green, Geoffrey L. Daniels

Immunohematology, Volume 9 , ISSUE 4, 105–108

Article | 17-February-2021

May the FORS be with you: a system sequel

reported to reactivate the Forssman synthase in humans and give rise to antigen expression is still c.887G>A. Although screening studies for this mutation have only been performed in small cohorts, it is quite safe to say that this phenotype is very rare. This supposition is also supported by data from the Genome Aggregation Database (gnomAD), in which 5 of 282,088 GBGT1 alleles carried c.887G>A (rs375748588) and 32 of 281,956 alleles were positive for c.886C>T (rs14104392). In a study by Abusibaa et

A.K. Hult, M.L. Olsson

Immunohematology, Volume 36 , ISSUE 1, 14–18

Article | 14-October-2020

Detection of granulocyte antibodies by flow cytometry without the use of pure granulocyte isolates

Established methods used to detect serum antibodies to granulocytes require the isolation of granulocytes. Flow cytometric analysis of granulocytes with monoclonal antibodies eliminates the need for granulocyte isolation. The purpose of this study was to develop a method to evaluate reactions of antibodies to granulocytes without separating granulocytes from other leukocytes. Three screening cell samples for granulocyte antibody detection were prepared from whole-blood samples in which the red

Karen M. Kiekhaefer, Karen M. Cipolone, Jo L. Procter, Kazuhiko Matsuo, David F. Stroncek

Immunohematology, Volume 17 , ISSUE 3, 70–75

Article | 09-October-2019

Applications of selected cells in immunohematology in a developing country: case studies

, they can also be used for several other immunohematologic applications. In a developing country like India, the performance of antibody screening for unexpected antibodies on a routine basis is a comparatively new phenomenon, and those laboratories performing advanced immunohematologic testing would need to use selected cells to arrive at an accurate conclusion. This report defines selected cells and enumerates sources of these RBCs. Detailed immunohematologic applications are discussed with

Ravi C. Dara Dara, Aseem Kumar Tiwari, Dinesh Arora, Subhasis Mitra, Geet Aggarwal, Devi Prasad Acharya, Gunjan Bhardwaj

Immunohematology, Volume 33 , ISSUE 1, 27–35

Article | 17-February-2021

A resource-conserving serologic and high-throughput molecular approach to screen for blood donors with an IN:−5 phenotype

attempted a screening program on our blood donors for INRA (IN:5) to find units of antigen-negative blood to meet the specific requirements for transfusion. Materials and Methods Human Research Subjects Blood samples from 5261 healthy volunteers, aged 18 years and older, were collected with informed consent at the Lok Samarpan Raktadan Kendra Blood Bank in Surat, Gujarat, India. Buffy coats were extracted from EDTA-anticoagulated whole blood samples. The buffy coats were pooled in batches of 20 and

S.R. Joshi, S.B. Senjaliya, K. Srivastava, W.A. Flegel

Immunohematology, Volume 36 , ISSUE 4, 129–132

Original Paper | 26-August-2016

Partial Characterization of Bacteriocin Produced by Halotolerant Pediococcus acidilactici Strain QC38 Isolated from Traditional Cotija Cheese

During a screening of lactic acid bacteria producing bacteriocin from Cotija cheese, the strain QC38 was isolated. Based on the 16S rRNA gene nucleotide sequencing (516 pb accession no KJ210322) and phylogenetic analysis, the isolate was identified as Pediococcus acidilactici. Neutralized cell-free supernatant was tested for antimicrobial activity against 17 Gram-negative and Gram-positive pathogens. Growth inhibition was achieved against Listeria monocytogenes (supplier or indication or source

Aurea I. Morales-Estrada, Ahidé López-Merino, Nestor Gutierrez-Mendez, Enrico A. Ruiz, Araceli Contreras-Rodríguez

Polish Journal of Microbiology, Volume 65 , ISSUE 3, 279–285

original-paper | 08-September-2020

A Polyclonal Spread Emerged: Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from the Intensive Care Unit in a Chinese Tertiary Hospital

ZHENGZHENG WANG, FANGYOU YU, XIAOFEI SHEN, MEILAN LI

Polish Journal of Microbiology, Volume 69 , ISSUE 3, 311–319

Case report | 01-December-2019

Alloimmunization to Kell blood group system antigen owing to unmatched blood transfusion in a resource-poor setting

One of the major drawbacks of multiple blood transfusions in patients with thalassemia is the risk of development of alloimmunization to various red cell antigens within blood group systems such as Rh, Kell, Duffy, and Kidd. The problem is greater in developing countries because of lack of awareness and insufficient availability of specific typing antisera and antibody screening panels owing to financial constraints. It is of utmost importance to provide D, C, c, E, e, and K phenotype-matched

Sheetal Malhotra, Gagandeep Kaur, Sabita Basu, Ravneet Ravneet, Geetanjali Jindal

Immunohematology, Volume 28 , ISSUE 2, 45–48

Article | 30-November-2018

A New Method of Improving the Traditional Traffic Identification and Accuracy

Wang Zhongsheng, Gao Jiaqiong

International Journal of Advanced Network, Monitoring and Controls, Volume 3 , ISSUE 3, 53–60

Article | 14-October-2020

Confirmation of positive antibody screens by solid-phase red cell adherence assay using a tube technique method with polyethylene glycol enhancement

manufacturers’ inserts. Of 49,084 samples, 313 (0.64%) were positive by the SPRCA assay. Of these, 99 (31.6%) samples remained positive when tested with PEG enhancement. The remaining 214 (68.4%) were negative, giving specificity for the SPRCA assay of 99.6 percent (48,985/ 49,199). We report a high specificity for antibody screening using the SPRCA assay. However, it is cost effective to perform a confirmatory tube test with PEG enhancement because 214 SPRCA assay samples were interpreted as having a

Richard R. Gammon, Michele Lake, Norberto D. Velasquez, Alicia Prichard

Immunohematology, Volume 17 , ISSUE 1, 14–16

original-paper | 01-May-2021

First Description of Various Bacteria Resistant to Heavy Metals and Antibiotics Isolated from Polluted Sites in Tunisia

) and Chemical Fertilizers *– CWTP: Collector between wastewater treatment plant. Sample collection and HM resistant bacteria screening. A plankton net was used to recover water samples, which were transferred into sterile bottles and transported at 4°C to the laboratory. After a first filtration step to remove insoluble solids, a nitrocellulose filter (0.45 μm) was used to collect microorganisms. Filters were directly placed on Lysogeny Broth (LB) agar plates with ampicillin (AMP) 64 μg/ml

SAMAR BEN MILOUD, OLFA DZIRI, SANA FERJANI, MUNTASIR MD ALI, MOHAMED MYSARA, ILHEM BOUTIBA, ROB VAN HOUDT, CHEDLY CHOUCHANI

Polish Journal of Microbiology, Volume 70 , ISSUE 2, 161–174

Article | 18-October-2020

From kill to overkill: 100 years of (perhaps too much) progress

Peter D. Issitt

Immunohematology, Volume 16 , ISSUE 1, 18–25

Report | 01-December-2019

Transfusion of D+ red blood cells to D– individuals in trauma situations

To conserve D– red blood cells (RBCs), our facility developed a policy for transfusion of D+ units to D– patients, particularly in trauma situations. To our knowledge, this is the first study looking at D-mismatched RBC transfusion in trauma patients. We developed guidelines for the transfusion of D-mismatched RBCs. Patients were followed by antibody screening and direct antiglobulin testing. Twenty-six patients were identified, and 57.7 percent of the cases were the result of

Amanda Tchakarov, Rhonda Hobbs, Yu Bai

Immunohematology, Volume 30 , ISSUE 4, 149–152

research-article | 30-November-2020

The risk for paediatric obstructive sleep apnoea in rural Queensland

discomfort for the parent and child to be hospitalised overnight, and long waiting times between referral and testing have made PSG impractical for routine clinical use and research purposes.21 Several screening questionnaires have been developed to improve OSA detection so that the health system is not unnecessarily strained.22 The Paediatric Sleep Questionnaire (PSQ) introduced by Chervin et al. has been validated against the gold standard of PSG, with a sensitivity of 0.85 and specificity of 0.87. It

Marguerite A. Fischer, Ersan I. Karadeniz, Carlos Flores-Mir, Daniel Lindsay, Carmen Karadeniz

Australasian Orthodontic Journal, Volume 37 , ISSUE 2, 197–205

Original Research | 18-July-2017

Diversity of Root-knot Nematodes Associated with Tubers of Yam (Dioscorea spp.) Established Using Isozyme Analysis and Mitochondrial DNA-based Identification

Abstract The root-knot nematodes (RKN), Meloidogyne spp., represent an important threat to yam (Dioscorea spp.) production in West Africa. With the aim to establish the diversity of RKN species affecting yam tubers, for control and resistance screening purposes, surveys were conducted in the main yam producing areas of Nigeria. Galled tubers (N = 48) were collected from farmers’ stores and markets in nine states in Nigeria and in one district in Ghana. RKN isolated from yam tubers were

Yao A. Kolombia, Gerrit Karssen, Nicole Viaene, P. Lava Kumar, Nancy de Sutter, Lisa Joos, Danny L. Coyne, Wim Bert

Journal of Nematology, Volume 49 , ISSUE 2, 177–188

Research Article | 20-May-2019

FRANCISELLA TULARENSIS – REVIEW 

variable intergenic regions may be also applicable to detection, differentiation and determination of genetic variation among F. tularensis strains. In addition, the above methods could be successfully used in molecular characterization of tularaemia strains from humans and animals isolated in screening research, and during epizootic and epidemic outbreaks.

Piotr Cieślik, Józef Knap, Agata Bielawska-Drózd

Postępy Mikrobiologii - Advancements of Microbiology, Volume 57 , ISSUE 1, 58–67

research-article | 30-November-2018

A loop-mediated isothermal amplification assay for the plant-parasitic nematode Aphelenchoides besseyi in rice seedlings

Jiue-in Yang, Guan-yi Yu

Journal of Nematology, Volume 51 , 1–11

original-paper | 27-December-2020

Bioinformatics Analysis of Key micro-RNAs and mRNAs under the Hand, Foot, and Mouth Disease Virus Infection

SHENG LIN, LIU YANG, SHIBIAO WANG, BIN WENG, MIN LIN

Polish Journal of Microbiology, Volume 69 , ISSUE 4, 479–490

research-article | 30-November-2019

Effects of HTR1A rs6295 polymorphism on emotional attentional blink

the advancement of societal health-care policies by genotyping based screening practices. Activity in the dorso-lateral prefrontal cortex (DLPFC) has been shown to be involved in the control of attention when responding to threatening stimuli in the context of emotional attentional blink (EAB) assessment (Peers et al., 2013). It is also known that depression is characterized by hypoactivity of the DLPFC (Groenewold et al., 2013) and targeting DLPFC with non-invasive neuromodulation such as

Kadi Tulver, Madis Bachmann, Mariliis Vaht, Jaanus Harro, Talis Bachmann

Acta Neurobiologiae Experimentalis, Volume 80 , ISSUE 4, 389–399

Article | 14-October-2020

Loss of enzyme-sensitive antigens due to the presence of leukocytes, neomycin sulfate, and LISS

Previous studies have shown that RBCs with residual WBCs stored in LISS and neomycin sulfate develop characteristics associated with enzyme-treated RBCs. During a mass screening program to antigen type donor RBCs, we observed that the Fya antigens on a RBC sample from an in-house panel became non-detectable with anti-Fya after incubation overnight in Diluent 2 from Micro Typing Systems, Inc. (MTS, Pompano Beach, FL). In response to this observation, we initiated an investigation to determine

Randall W. Velliquette, Paula Howard, Harry Malyska, Marion E. Reid

Immunohematology, Volume 19 , ISSUE 4, 109–111

Article | 18-October-2020

Comparison of tube and gel techniques for antibody identification

There are several methods for antibody detection and each technique has advantages and limitations. We compared the performance of the tube (polyethylene glycol–indirect antiglobulin test [PEG-IAT]) and gel test technique for antibody identification. From January to May 1999, we performed antibody screening tests by gel and tube techniques on 10,123 random blood samples submitted to our reference laboratory. Six hundred and twentyeight (6.2%) reactive samples were tested for antibody

Marcia Cristina Zago Novaretti, Eduardo Jens Silveira, Edio da Costa Filho, Pedro Enrique Dorlhiac- Llacer, Dalton de Alencar Fischer Chamone

Immunohematology, Volume 16 , ISSUE 4, 138–141

Article | 26-October-2020

Anti-Lu9: the finding of the second example after 25 years

:-1,2,6,9 antibody-screening red blood cells (RBCs) using either a low-ionic-saline additive solution or polyethylene glycol for enhancement. Lu:6,9 RBCs were reactive with the serum when ficin- or EDTA/glycine-acid-treated, but nonreactive when trypsin- or α-chymotrypsin-treated. Six known examples of Lu:9 RBCs were reactive with the GR serum. His serum did not contain anti-Lua, anti-HLA-B7 (-Bga) or antibodies to 34 low-incidence antigens tested. We have identified the second example of anti-Lu9

Kayla D. Champagne, Marilyn Moulds, Jo Schmidt

Immunohematology, Volume 15 , ISSUE 3, 113–116

Article | 03-November-2020

A comprehensive IgA service provided by a blood transfusion center

. By screening new donors and directed call-up, group O, D– red blood cell (RBC) units are always in stock. During 1 year, the center supplied 79 units of RBCs and 64 units of fresh frozen plasma to a variety of patients with IgA deficiency, including three undergoing liver transplantation. The center also provides a reference service for IgA/anti-IgA status. The technique used (hemagglutination inhibition) has a sensitivity well below the threshold of standard quantitation methods. Samples

R. Munks, J.R. Booth, R.J. Sokol

Immunohematology, Volume 14 , ISSUE 4, 155–160

Article | 03-November-2020

K phenotyping using a PK-7200 automated analyzer

K (Kell) is one of the most immunogenic of the red blood cell (RBC) antigens. In order to select K− RBC units, we developed K phenotyping on the Olympus PK-7200 equipment to save labor, time, and costs. The Olympus PK-7200 is fully automated equipment used primarily for blood typing and syphilis screening. We tested 3,587 blood donor samples in EDTA using a commercial anti-K serum diluted in HP Hemagen Power SolutionR(1:40). The equipment was set to prepare a 1.7% RBC suspension in

Marcia C. Zago-Novaretti, Silvana P. Navarro, Pedro E. Dorlhiac-Llacer, Dalton A.F. Chamone

Immunohematology, Volume 14 , ISSUE 1, 22–25

Article | 17-November-2020

Analysis ofthe routine use of polyethylene glycol (PEG) as an enhancement medium

) involving an anti-K that was not detected with LISS but was retrospectively found to be reactive with PEG, an additional 151 samples received for antibody screening were prospectively evaluated in parallel using PEG and LISS. PEG detected all clinically significant antibodies in the 50 previously tested samples, with mean reactivity scores greater than LISS or A-IAT. In the prospective study, PEG detected 35 clinically significant antibodies and 10 clinically insignificant antibodies, while LISS

Vicki J. Barrett, James R. Stubbs, Karen Stuardi, Angela Hollis, Leslie Clear

Immunohematology, Volume 11 , ISSUE 1, 11–13

research-article | 30-November-2018

Screening of Cucurbita maxima and Cucurbita moschata Genotypes for Resistance Against Meloidogyne arenaria, M. incognita, M. javanica, and M. luci

Twenty Cucurbita genotypes having potential use as rootstocks or for breeding programs were evaluated to identify resistance levels against different RKN species in pot experiments. The screening of these genotypes for resistance is of particular interest in overcoming the lack of available genetic sources to control RKN populations. Resistance is defined as the ability of a plant to significantly reduce nematode reproduction and is a relative concept because the level of nematode resistance in a

Gökhan Aydınlı, Ertan Sait Kurtar, Sevilhan Mennan

journal of nematology, Volume 51 , 1–10

Review | 09-October-2019

The Vel blood group system: a review

. Clinically, anti-Vel is important and has caused severe transfusion reactions, although hemolytic disease of the fetus and newborn caused by anti-Vel is uncommon. However, while screening for Vel– blood donors has become easier, the function of SMIM1 is still unknown, and despite its well-conserved sequence across the animal kingdom, the enigma continues.

Jill R. Storry, Thierry Peyrard

Immunohematology, Volume 33 , ISSUE 2, 56–59

research-article | 19-March-2020

Natural occurrence and distribution of entomopathogenic nematodes (Steinernematidae, Heterorhabditidae) in Viti Levu, Fiji Islands

Sumeet Kour, Uma Khurma, Gilianne Brodie, Selcuk Hazir

journal of nematology, Volume 52 , 1–17

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