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Report | 01-December-2019

Single-center comparison of gel microcolumn and solid-phase methods for antibody screening

Our facility changed antibody screening methods from a gel microcolumn–based test (ID-Micro Typing System Gel Test; Ortho Clinical Diagnostics, Inc., Raritan, NJ) to an automated solid-phase test (Galileo/Capture-R Ready-Screen [I and II], Immucor, Inc., Norcross, GA). To determine whether detection rates for commonly encountered clinically significant red blood cell antibodies differed as a consequence of this change, preimplementation and postimplementation antibody identification

Anne Schmidt, Brenda J. Bendix, Eapen K. Jacob, Sandra C. Bryant, James R. Stubbs

Immunohematology, Volume 29 , ISSUE 3, 101–104

Report | 01-December-2019

Performance characteristics of two automated solid-phase red cell adherence systems for pretransfusion antibody screening:  a cautionary tale

Our institution has implemented two instruments, the Galileo and the Echo, that use different solid-phase red cell adherence assays for antibody screening in pretransfusion compatibility testing. During the initial implementation of these two instruments, we noticed very different problems: falsely positive results on the Galileo, and falsely negative results and lack of reproducibility on the Echo. Comparison of falsely positive antibody screen results from approximately equivalent numbers of

Karen Quillen, James Caron, Kate Murphy

Immunohematology, Volume 28 , ISSUE 4, 137–139

Article | 09-November-2020

Comparison of hemagglutination and solid phase titration methods for determination of critical prenatal antibody titers

The hemagglutination (HA) tube method is the standard method for determination of antibody titer in prenatal samples. Most facilities use a titer between 8 and 32 as their definition of a critical value when amniocentesis may be considered. This study determined if there is a relationship between the results of HA tube and solid phase (SP) titers performed on the same sample. Forty-six paired samples containing known antibody were titrated by both HA tube and SP methods and the results

Brenda C. Alder, Stephanie H. Summers, Virginia Hare

Immunohematology, Volume 13 , ISSUE 3, 84–89

Article | 14-October-2020

Evaluation of a new solid-phase immunoassay for alloantibody detection using bromelin-treated and untreated red blood cells

The enzyme test is used to detect certain antibodies or facilitate antibody identification. This study compares antibody reactivity with bromelin-treated red blood cells (RBCs) and untreated RBCs using a newly developed solid-phase immunoassay. The reactivity of irregular antibodies was tested by a magnetic-mixed passive hemagglutination assay (M-MPHA). In addition, antibody reactivity was tested with dried stroma of bromelin-treated RBCs and untreated RBCs (M-MPHA-Dry). Rh antibodies were

Toyohiro Tamai, Toshio Mazda

Immunohematology, Volume 17 , ISSUE 1, 17–21

Article | 14-October-2020

Confirmation of positive antibody screens by solid-phase red cell adherence assay using a tube technique method with polyethylene glycol enhancement

Our blood bank routinely screens donors for antibodies using a solid-phase red cell adherence (SPRCA) assay. Positive results are then confirmed using a tube technique with polyethylene glycol (PEG) enhancement due to reported higher specificity than with SPRCA. Over a 5-month period, 49,084 donor serum or plasma samples were tested using the SPRCA assay. Further identification of positive samples was performed using a PEG enhancement method. Testing was performed with strict adherence to the

Richard R. Gammon, Michele Lake, Norberto D. Velasquez, Alicia Prichard

Immunohematology, Volume 17 , ISSUE 1, 14–16

Report | 12-March-2020

Determination of optimal method for antibody identification in a reference laboratory

Methods commonly used for antibody identification are hemagglutination (tube), column agglutination (gel), and solid-phase red cell adherence. Our AABB immunohematology reference laboratory (IRL) conducted a study to determine which antibody identification testing method was optimal for detecting all clinically significant antibodies. Patient specimens were sent to our IRL from August 2008 to September 2009. Routine testing was performed by tube method and then by manual gel and manual solid

Jennifer R. Haywood, Marilyn K. Grandstaff Moulds, Barbara J. Bryant

Immunohematology, Volume 27 , ISSUE 4, 146–150

Report | 16-October-2019

Method-specific and unexplained reactivity in automated solid-phase testing and their association with specific antibodies

The inherent tradeoff between sensitivity and specificity in the detection of unexplained antibodies has been the objective of many studies, editorials, and journal articles. Many publications note that no method is capable of detecting all clinically significant antibodies while avoiding all clinically insignificant antibodies. This study describes the frequency of nonspecific reactivity and unexplained reactivity in solid-phase testing, along with the subsequent development of specific

Mary E. Harach, Joy M. Gould, Rosemary P. Brown, Tricia Sander, Jay H. Herman

Immunohematology, Volume 34 , ISSUE 3, 93–97

Article | 14-December-2020

Antibody detection using pooled sera and a solid phase system

The purpose of the study was to evaluate the feasibility of substituting the Immucor Capture™-R solid phase (SP) antibody detection system for our routine donor antibody screen. Our routine procedure (RP) used a 12-drop pool of six donor sera and one drop of pooled reagent red cells, with 37°C incubation and indirect antiglobulin test readings. The SP system was used according to the manufacturer’s directions except that one drop of the pooled sera (rather than an individual

Malcolm L. Beck, Jill T. Hardman, Alicia M. Briseño

Immunohematology, Volume 7 , ISSUE 3, 73–75

Article | 20-December-2020

Red cell antibody identification by solid phase red cell adherence utilizing dried RBC monolayers

Recent technological advances in the immobilization and drying of red cell monolayers for use in solid phase red cell adherence (SPRCA) assays have resulted in the development of reagent red cells for antibody screening and identification that are stable at room temperature. Panels consisting of twelve different RBC samples dried onto individual microplate wells were evaluated with 176 samples whose antibody specificities had previously been determined by conventional hemagglutination

Darryl L. Stone, Ralph A. Eatz, Susan D. Rolih, Seaborn J. Farlow, Gordon S. Hudson, Lyle T. Sinor

Immunohematology, Volume 6 , ISSUE 1, 12–17

Article | 17-November-2020

Detection of Lewis, P1, and some MNS blood group system antibodies by a solid phase assay

Some solid phase red cell adherence (SPRCA) assays are designed to detect IgG antibodies to red blood cell (RBC) antigens. These assays use anti-IgG-coated red cells as the indicator. It is reported that most antibodies to Lea, Leb, P1, M, and N fail to react by solid phase (SP), presumably because they are IgM antibodies. Those detected are assumed to be IgG. In one year, during routine testing using SPRCA to screen patients for intended RBC transfusion, 28 of 59 such examples were found

Susan Rolih, Ronald Thomas, Lyle Sinor

Immunohematology, Volume 11 , ISSUE 3, 78–80

Article | 17-November-2020

A comparison of two solid phase systems for antibody detection

Two solid phase methods of antibody detection, Capture-R (CR) and Capture-R Ready-Screen (RS), were compared to determine their acceptability for use in prenatal antibody screening. Ninety-six serum samples, screened using a saline antiglobulin test, were coded and tested by CR and RS at two laboratory sites using a blinded study design. Thirty of the samples were free of antibody, and 66 samples contained antibody. Parallel testing was also performed in both laboratories on 648 prenatal

Gwen M. Haslam, Margaret Persaud, Yvette Fournier, Joanne Sajur, Janice Aulph, Nancy Heddle

Immunohematology, Volume 11 , ISSUE 1, 8–10

Case report | 17-November-2020

Case report: solid-phase platelet crossmatching to support the alloimmunized patient

Platelet crossmatching by a solid-phase red cell adherence assay was used to provide compatible platelets for two alloimmunized patients with leukemia. In this study, a successful platelet transfusion was defined as giving a corrected count increment (CCI) of >7,500 in a posttransfusion sample. For patient A, a total of 205 random platelet concentrates (PCs) were crossmatched. Eleven were considered compatible. These 11 PCs were transfused during five transfusion episodes. Four of the five

Bernadette A. O’Connell

Immunohematology, Volume 11 , ISSUE 4, 150–152

Article | 14-October-2020

Enzyme and DTT treatment of adherent RBCs for antibody identification by a solid phase immunoassay system

Toyohiro Tamai, Toshio Mazda

Immunohematology, Volume 18 , ISSUE 4, 114–119

Article | 17-November-2020

Detection of drug-dependent platelet antibodies by use of solid-phase red cell adherence techniques

solid-phase red cell adherence assay was used to detect drug-dependent platelet antibodies active by either the immune complex or the hapten mechanism. Three cases were evaluated for the presence of drug-dependent platelet antibodies. Two patients presented with thrombocytopenia that could not be attributed to other causes. The third case was evaluated for the presence of drug-dependent antibodies after poor responses to platelet transfusions. In these three cases, discontinuation of the implicated

Miriam F. Leach, Linda K. Cooper, James P. AuBuchon

Immunohematology, Volume 11 , ISSUE 4, 143–149

Article | 10-November-2020

Detection of tube agglutination 37°C-only antibodies by solid-phase red cell adherence

There are no published data on the detection of tube agglutination (TA) 37°C-only antibodies by solid-phase (SP) red cell adherence assays using anti-IgG-coated indicator red cells. Thirteen examples of TA 37°C-onIy antibodies were tested by conventional SP methods. Four TA 37°C-only antibodies failed to react by SP. Three were anti-Lea, considered clinically insignificant, and one was anti-E, an anti­body of potential clinical significance. The remaining nine TA 37°C-only

Susan Rolih, Fern Fisher, Dolores Fiqueroa, Gwenn Lindsay

Immunohematology, Volume 12 , ISSUE 1, 27–29

Article | 03-November-2020

Rapid screening of platelet donors for PlA1 (HPA-1a) alloantigen using a solid-phase microplate immunoassay

effectiveness of a solid-phase microplate immunoassay for this purpose. Platelet-rich donor plasmas were tested using the Capture-P® kit (Immucor, Norcross, GA). Platelet monolayers in microtiter wells were incubated with anti-PlA1, washed, and exposed to red blood cells (RBCs) precoated with anti-human IgG. Adherence of RBCs in a diffuse pattern across the well surface indicated the attachment of anti-PlA1 to PlA1-positive platelets whereas sedimentation of unattached RBCs into a central pellet

Jo L. Procter, Faye E. Vique, Ed Alegre, Junichi Honda, Kazuhiko Matsuo, Diane Reid

Immunohematology, Volume 14 , ISSUE 4, 141–145

Article | 26-October-2020

Naturally-occurring anti-Jka in infant twins

Anti-Jka was detected by solid-phase red cell adherence (SPRCA) anti-body detection and identification tests in the plasma of a 9-month-old female infant during a routine presurgical evaluation. The patient and her nonidentical twin sister who also had anti-Jka in her plasma, were products of an uncomplicated in vitro fertilization, full-term pregnancy and vaginal delivery. Neither twin had been transfused, recently infected, or treated with medication. Their mother had no prior pregnancies or

Dawn H. Rumsey, Sandra J. Nance, Mary Rubino, S. Gerald Sandler

Immunohematology, Volume 15 , ISSUE 4, 159–162

Article | 15-April-2020

In search of the Holy Grail: comparison of antibody screening methods

improved sensitivity,with the occasional negative impact on relevant results. The focus on improving efficiency through automation, and personnel resourcing challenges of the transfusion service,have led laboratories to select methods tailored to meet their needs. This review compares the newer methods used in the gel test and solid phase test with commonly used tube methods. Both of the newer methods were developed with future adaptation to automation in mind. Further literature reviews about

Tony S. Casina

Immunohematology, Volume 22 , ISSUE 4, 196–202

Article | 18-October-2020

Comparison of human platelet antigen (HPA)-1a typing by solid phase red cell adherence to HPA-1 allotypes determined by allelespecific restriction enzyme analysis

Phenotype results for human platelet antigen (HPA)-1 by CaptureP®‚ (Immucor, Inc., Norcross, GA) solid phase red cell adherence (SPRCA) were compared to results of allele-specific restriction enzyme analysis (ASRA) for the determination of HPA-1 allotype. Because the expression of HPA-1a and HPA-1b is determined by a single nucleotide substitution of thymine‘cytosine at position 196 of the gene encoding membrane glycoprotein (GP)-IIIa, it is possible to distinguish the alternate

Michael J. McGann, Jo L. Procter, Junichi Honda, Kazuhiko Matsuo, David F. Stroncek

Immunohematology, Volume 16 , ISSUE 2, 68–73

Case report | 01-December-2019

Performance of an automated solid-phase  red cell adherence system compared with  that of a manual gel microcolumn assay for  the identification of antibodies eluted from  red blood cells

IgG antibodies coating red blood cells (RBCs) can be removed by elution procedures and their specificity determined by antibody identification studies. Although such testing is traditionally performed using the tube agglutination assay, prior studies have shown that the gel microcolumn (GMC) assay may also be used with comparable results. The purpose of this study was to compare an automated solid-phase red cell adherence (SPRCA) system with a GMC assay for the detection of antibodies eluted

Rachel H. Finck, Rebecca J. Davis, Shih-Mao Teng, Dennis Goldfinger, Alyssa F. Ziman, Qun Lu, Shan Yuan

Immunohematology, Volume 27 , ISSUE 1, 1–5

Article | 06-December-2020

Antibody detection errors due to acidic or unbuffered saline

Isotonic saline solutions, buffered with potassium phosphate or sodium phosphate salts, were evaluated in parallel with unbuffered saline to determine if they improved antibody detection by solid phase red cell adherence or hemagglutination methods. Saline buffered to a pH of 7.0 to 7.5, when used to suspend red cells or to wash sensitized red cells in preparation for the antiglobulin test, produced the best positive solid phase and hemagglutination results. The pH range of commercially

Susan Rolih, Ron Thomas, Fern Fisher, Joanne Talbot

Immunohematology, Volume 9 , ISSUE 1, 15–18

Original Paper | 09-October-2019

Use of standard laboratory methods to obviate routine dithiothreitol treatment of blood samples with daratumumab interference

, dithiothreitol (DTT) treatment of the patient’s RBCs should be performed. The medical charts of four patients treated with daratumumab were reviewed. The individual number of doses ranged from 1 to 14; patient age ranged from 55 to 78 years; two men and two women were included in the review. Type and screen data were obtained from samples collected over 33 encounters with a range of 1 to 13 encounters per patient. All samples were tested initially by automated solid-phase testing. Any reactivity with

Nicholas J. Lintel, Debra K. Brown, Diane T. Schafer, Farai M. Tsimba-Chitsva, Scott A. Koepsell, Sara M. Shunkwiler

Immunohematology, Volume 33 , ISSUE 1, 22–26

Article | 17-November-2020

Trimeresurus venom inhibition of anti-HPA-1a and anti-HPA-1b antibody binding to human platelets

A solid-phase red cell adherence assay was used to demonstrate the specific inhibitory effect of seven species of Trimeresurus snake venom on the binding of HPA-1a- and HPA-1b-specific platelet antibodies. Trimeresurus venom did not inhibit the binding of HLA-, HPA-3a-, HPA-3b-, HPA-4a-, HPA-5a-, and HPA-5b-specific platelet antibodies. Venom from other genera of snakes, including representatives from Agkistrodon, Ancistrodon, Bitis, Bothrops, Bungarus, Causus, Crotalus, Dendroaspis, Ecis

Steve J. Wlodar, Darryl L. Stone, Lyle T. Sinor

Immunohematology, Volume 11 , ISSUE 4, 129–132

Review | 20-March-2020

Detection and identification of platelet antibodies and antigens in the clinical laboratory

As a result of the unique functional properties of platelets, morerobust methods were required for detection of antibodies raised against them. Immunofluorescence detection by flow cytometry, solid-phase red cell adherence, and antigen capture ELISAs are some of the current tests that have been developed to meet the challenges of platelet antibody detection and identification and antigen phenotyping. Recently developed protein liquid bead arrays are becoming the next-generation platelet

Brian R. Curtis, Janice G. McFarland

Immunohematology, Volume 25 , ISSUE 3, 125–135

Article | 09-November-2020

Semiautomation of platelet HPA-1a phenotyping by SPRCA and ELISA

An enzyme-linked immunosorbent assay (ELISA) and solid phase red cell adherence assay (SPRCA) were assessed for platelet HPA-1a typing in U well microplates. Both methods were partially automated by the use of the Tecan RSP 8051ID robotic sampler and the SLT 400 ATC plate reader with Soft2000 software. Pretreatment of the adherent platelets with chloroquine diphosphate or citric acid enabled anti-HPA-1a, even when contaminated with HLA class 1 antibodies, to be used for typing. Of 675 antenatal

Lionel A. Mohabir, Lynne Porter

Immunohematology, Volume 13 , ISSUE 2, 44–48

Case report | 09-October-2019

Postpartum acute hemolytic transfusion reactions associated with anti-Lea in two pregnancies complicated by preeclampsia

Lewis blood group antibodies, which are mostly naturally occurring and considered clinically insignificant, have rarely been documented as a cause of acute hemolytic transfusion reactions (AHTRs). This report presents two cases of AHTRs caused by anti-Lea occurring in postpartum black females (one group B, one group AB) whose pregnancies were complicated by preeclampsia. Neither anti-Lea was detected by automated solid-phase red cell adherence technology in pre-transfusion testing. Therefore

Marcia Marchese

Immunohematology, Volume 33 , ISSUE 3, 114–118

Review | 09-October-2019

How to recognize and resolve reagentdependent reactivity: a review

Gavin C. Patch, Charles F. Hutchinson, Nancy A. Lang, Ghada Khalife

Immunohematology, Volume 32 , ISSUE 3, 96–99

Article | 18-October-2020

From kill to overkill: 100 years of (perhaps too much) progress

Peter D. Issitt

Immunohematology, Volume 16 , ISSUE 1, 18–25

Case report | 26-October-2019

Weak D type 67 in four related Canadian blood donors

donors are investigated by other methods. We describe four weak D type 67 (RHD*01W.67) donors whose samples tested as D– by automated microplate and manual methods but were later determined to be D+ by automated solid-phase and RHD gene analysis. Solidphase serologic and molecular typing results of all four donors were identical. It was identified that the donors are of EnglishIrish descent; two are brothers and the others are cousins.  Transfusion of blood from one of these donors likely

Philip Berardi, Emma Bessette, Michiko Ng, Nancy Angus, Debra Lane, Lise Gariepy, Katerina Pavenski, Gorka Ochoa-Garay, Jacqueline Cote, Mindy Goldman

Immunohematology, Volume 31 , ISSUE 4, 159–162

research-article | 30-November-2017


of organic compounds occurring in swimming pool water. The tests were performed under laboratory conditions by the use of two UV lamps with a different irradiation spectrum. To examine the effectiveness of the UV irradiation process, chromatographic analysis preceded by solid-phase extraction was used. 2. EXPERIMENTAL 2.1. Research methodology The subject of the study was swimming pool water collected from an indoor sport pool. The implemented water treatment system based on coagulation


Architecture, Civil Engineering, Environment, Volume 11 , ISSUE 3, 131–138

Article | 26-October-2020

Provision of HPA-1a (PlA1)-negative platelets for neonatal alloimmune thrombocytopenia: screening, testing, and transfusion protocol

HPA-1a-negative platelet products are not routinely available for newborns with alloimmune thrombocytopenia. In this article we describe a program established to identify normal pheresis donors who are HPA-1a-negative and to organize their future donations so that our regional blood center would always have an HPA-1a-negative platelet product available. The solid phase red cell adherence assay was used for initial screening of platelet pheresis products. HPA-1a-negative donors were confirmed

Mary Munizza, Sandra Nance, Maryann Keashen-Schnell, William Sherwood, Scott Murphy

Immunohematology, Volume 15 , ISSUE 2, 71–74

Article | 14-October-2020

Equivalence of spray-dried K2EDTA,spray-dried K3EDTA, and liquid K3EDTA anticoagulated blood samples for routine blood center or transfusion service testing

from two different manufacturers. All patients’samples were tested in parallel by solid phase/microplate method (Immucor® ABS 2000) and the standard manual tube method. All test results for routine blood bank tests on donors’ and patients’ samples were concordant, demonstrating the equivalence of spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes for routine donor center or transfusion service testing.

Stacie Leathem, Nicole Dodge Zantek, Marti Kemper, Laura Korte, Al Langeberg, S. Gerald Sandler

Immunohematology, Volume 19 , ISSUE 4, 117–121

Article | 17-November-2020

Application of the proteolytic enzyme papain in routine platelet serology

, -5a, -5b, and -Naka were examined. HLA antibodies were also included. All sera were tested by a solid-phase red cell adherence technique in parallel with untreated platelets (UP) and platelets treated with papain (PP) for 15 minutes at 37°C. The reactivity of anti-HPA-2b was eliminated and that of anti-HPA-3a was either eliminated or almost eliminated with PP. Antisera specific for the other alloantigens tested reacted similarly or more strongly with PP compared with UP. These findings were

John A.G. Lown, Brian J. Dale

Immunohematology, Volume 11 , ISSUE 4, 140–142

Report | 14-October-2020

Development of anti-Bw6 reactivity in patients receiving r-GCSF: a preliminary report

anomalous reactions in solid phase red cell adherence (SPRCA) assays. SPRCA tests were positive only when platelets were adhered to the polystyrene plates in the presence of glucose; when other simple sugars were used, the patients’ sera failed to react. HLA Bw6-positive platelets were more likely than HLA Bw6-negative platelets to be reactive (þ < .001). The transfusion of HLA Bw6-positive platelets to patients displaying this in vitro reactivity (positive patients) resulted in a 50

Miriam Fogg Leach, James P. AuBuchon

Immunohematology, Volume 17 , ISSUE 3, 63–69

Article | 26-October-2020

Frequency of thrombocytopenia associated with gentamicin therapy

Many drugs, including antibiotics such as gentamicin, have been associated with the development of a drug-induced thrombocytopenia. Serologic methods for detection of drug-dependent plateIet antibod­ies (DDPAs) are not routinely performed, and the incidence of such antibody-mediated thrombocytopenia is not known. As we routinely perform solid-phase red cell adherence assays for the detection of DDPAs, a study was designed to determine the incidence of gentam­icin-associated platelet

Miriam Fogg Leach, James P. AuBuchon

Immunohematology, Volume 15 , ISSUE 4, 167–170

Report | 01-December-2019

Identifying D-positive donors using a second automated testing platform

Because of the variability of D expression, one method may be inadequate to correctly classify donors with variant RHD alleles. We evaluated the use of a solid-phase automated platform (ImmucorGamma Galileo) to confirm D– test results obtained on first-time donors on the Beckman Coulter PK7300 automated microplate test system. Samples with discordant results were analyzed by serologic tube methods, RHD genotyping using the BLOODchip platform (Progenika), and, if necessary, sequencing. We

Mindy Goldman, Ilona Resz, Jacqueline Cote, Gorka Ochoa, Nancy Angus

Immunohematology, Volume 29 , ISSUE 3, 97–100

Review | 09-October-2019

A Caucasian JK*A/JK*B woman with Jk(a+b–) red blood cells, anti-Jkb, and a novel JK*B allele c.1038delG

The Kidd blood group on the red blood cell (RBC) glycoprotein urea transporter-B has a growing number of weak and null alleles in its gene SLC14A1 that are emerging from more widespread genotyping of blood donors and patients. We investigated a 64-year-old Caucasian woman of Polish-Czech descent who developed anti-Jkb detected in solid-phase RBC adherence testing within 12 days after 7 units of RBCs were transfused. Her RBCs subsequently typed Jk(a+b–) by licensed reagents and human

Glenn Ramsey, Ricardo D. Sumugod, Paul F. Lindholm, Jules G. Zinni, Jessica A. Keller, Trina Horn, Margaret A. Keller

Immunohematology, Volume 32 , ISSUE 3, 91–95

Article | 14-October-2020

Neonatal alloimmune thrombocytopenia due to anti-HPA-2b (anti-Koa)

of the hematoma was performed after transfusion. Testing with a solid phase ELISA revealed reactivity against GP1b/IX. MAIPA testing after platelet treatment with the protease inhibitor leupeptin demonstrated the presence of anti-HPA-2b. On PCR-SSP the mother was HPA-2a homozygous, the father was HPA-2a/2b. Antibodies against the HPA-2b antigen located on the GP1b/IX complex have been reported in rare cases of NAIT. Testing is complicated by proteolytic degradation of the antigen-bearing fragment

Mindy Goldman, Élise Trudel, Samir Khalife, Gwendoline M. Spurll

Immunohematology, Volume 19 , ISSUE 2, 43–46

Article | 03-November-2020

Immunoprophylaxis using intravenous Rh immune globulin should be standard practice when selected D-negative patients are transfused with D-positive random donor platelets

A 67-year-old female developed excessive bleeding and thrombocytopenia following cardiovascular surgery. Her blood type was group A, D–. The only platelet products available in the transfusion service were random donor platelet concentrates from D+ donors. She was transfused with a pool of 6 D+ random donor platelet concentrates. Anti-D undetected in her pretransfusion serum by solid-phase antibody screen was present 11 days later. Retrospectively, the patient provided a history of having

Clinton A. Ewing, Dawn H. Rumsey, Albert F. Langeberg, S. Gerald Sandler

Immunohematology, Volume 14 , ISSUE 4, 133–137

Article | 20-December-2020

A simple method for inhibiting ABO antibodies in sera used for platelet crossmatching

by Neutr-AB when tested against group A or B platelets by solid-phase ELISA. The amount of A antigen on platelets and the levels of anti-A in group B or O sera can vary considerably, so we investigated the extent of the problem in platelet crossmatching. Sixty percent of 20 random group O sera reacted with platelets from a donor, who was selected because of the strong A antigen present on the platelets. Eighty-six percent of randomly selected group A platelets reacted with a group O serum

Nina Postoway, George Garratty

Immunohematology, Volume 6 , ISSUE 3, 68–70

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