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  • Immunohematology

 

Article | 16-November-2020

Empirical evaluation of the transfusion medicine tutor

Transfusion Medicine Tutor (TMT). The results show that the students who were taught by an instructor using TMT to provide the instructional environment went from 0 percent correct on a pretest case to 87 percent correct on posttests (n = 15). This increase compares with an improvement rate of 20 percent by a control group (n = 15) who used a passive version of the system with the tutoring functions turned off.

Jodi Heintz Obradovich, Philip J. Smith, Stephanie Guerlain, Sally Rudmann, Patricia Strohm, Jack Smith, John Svirbely, Larry Sacks

Immunohematology, Volume 12 , ISSUE 4, 169–174

Report | 25-March-2020

The potential of blood group genotyping for transfusion medicine practice

Molecular diagnostics is the fastest growing area of clinical laboratory medicine.  The ability to rapidly amplify genes of bacterial, viral, or human origin, and the development of DNA array platforms, are driving a technology revolution in the clinical laboratory.  A DNA-based testing approach is particularly applicable to blood bank and transfusion medicine for rapid, cost-effective antigen typing.  Experience with DNA-based methods during the past decade has shown that these

Connie M. Westhoff

Immunohematology, Volume 24 , ISSUE 4, 190–195

Review | 28-April-2020

Review: complement receptor 1 therapeutics for prevention of immune hemolysis

The complement system plays a crucial role in fighting infections and is an important link between the innate and adaptive immune responses. However, inappropriate complement activation can cause tissue damage, and it underlies the pathology of many diseases. In the transfusion medicine setting, complement sensitization of RBCs can lead to both intravascular and extravascular destruction. Moreover, complement deficiencies are associated with autoimmune disorders, including autoimmune hemolytic

Karina Yazdanbakhsh

Immunohematology, Volume 21 , ISSUE 3, 109–118

Book Review | 14-December-2020

BOOK REVIEW: Handbook of Transfusion Medicine

James P. AuBuchon

Immunohematology, Volume 7 , ISSUE 3, 77–78

Book Review | 14-October-2020

BOOK REVIEW: Information Technology in Transfusion Medicine

S. Gerald Sandler

Immunohematology, Volume 19 , ISSUE 3, 93–93

Book Review | 14-October-2020

BOOK REVIEW: Policy Alternatives in Transfusion Medicine

Christopher D. Hillyer

Immunohematology, Volume 18 , ISSUE 3, 86–86

Book Review | 14-October-2020

BOOK REVIEW: Practical Guide to Transfusion Medicine

S. Gerald Sandler

Immunohematology, Volume 18 , ISSUE 2, 48–48

Book Review | 14-October-2020

BOOK REVIEW: Evidence-Based Practice of Transfusion Medicine

Ruth Mougey

Immunohematology, Volume 19 , ISSUE 1, 26–26

Book Review | 14-October-2020

BOOK REVIEW: Cellular Therapy: New Frontiers in Transfusion Medicine

John D. Roback

Immunohematology, Volume 19 , ISSUE 2, 62–62

Introduction | 26-October-2019

The role of red cell genotyping in transfusion medicine

Margaret A. Keller

Immunohematology, Volume 31 , ISSUE 2, 49–52

Case report | 01-December-2019

Posttransplant maternal anti-D: a case study and review

Lisa Senzel, Cecilia Avila, Tahmeena Ahmed, Harjeet Gill, Kim Hue-Roye, Christine Lomas-Francis, Marion E. Reid

Immunohematology, Volume 28 , ISSUE 2, 55–59

Article | 17-February-2021

An outcome-based review of an accredited Specialist in Blood Banking (SBB) program: 25 years and counting

Committee on Accreditation reviews these reports and correction plans and provides feedback allowing for ongoing process improvement. With continuous advancements in transfusion medicine, educational programs such as SBB programs and Transfusion Medicine fellowship programs may benefit from a periodic internal review of their program’s performance. This type of review provides data to analyze and determine distinct characteristics and attributes of their program. When data are available for review

K.M. Byrne, T.D. Paige, W.A. Flegel

Immunohematology, Volume 36 , ISSUE 1, 7–13

Book Review | 26-October-2020

BOOK REVIEW: Emerging Legal Issues in Blood Banking and Transfusion Medicine

William C. Sherwood

Immunohematology, Volume 15 , ISSUE 1, 52–53

Book Review | 26-October-2020

BOOK REVIEW: Emerging Legal Issues in Blood Banking and Transfusion Medicine (reprint)

William C. Sherwood

Immunohematology, Volume 15 , ISSUE 2, 81–81

Article | 15-April-2020

Transfusion practices for patients with sickle cell disease at a major academic medical center

care units),cardiothoracic surgery, pediatrics, the operating room, the emergency department, labor and delivery, dialysis, and outpatient services. UNC is recognized for its expertise in coagulation,transfusion medicine,and hematology,particularly in sickle cell disease (SCD). The sickle cell center at UNC,which began in 1980 and continues today,in conjunction with our neighboring institution,Duke University Medical Center,is designated as part of a National Institutes of Health comprehensive

Araba Afenyi-Annan, Nicholas Bandarenko

Immunohematology, Volume 22 , ISSUE 3, 103–107

Article | 14-October-2020

Blood group antigen profile predicted by molecular biology - use of real-time polymerase chain reaction to genotype important KEL, JK, RHD, and RHCE alleles

The most clinically important blood group systems in transfusion medicine, excluding the ABO system, are the RH, Kell, and Kidd systems. Alloantibodies to antigens of these systems may be produced following blood transfusion or during pregnancy and can result in serious hemolytic transfusion reactions and hemolytic disease of the newborn.We developed rapid and robust techniques for RHD, RHCE, KEL, and JK genotyping with the use of a real-time polymerase chain reaction instrument. Two

Fernando Manuel Ferreira Araújo, Christiana Pereira, Fátima Monteiro, Isabel Henriques, Elsa Meireles, Pedro Lacerda, Ana Aleixo, Regina Celeste, Luis M. Cunha-Ribeiro, Maria J. Rodrigues

Immunohematology, Volume 18 , ISSUE 3, 59–64

Case report | 13-April-2020

Case report: DNA testing resolves unusual serologic results in the Dombrock system

Diane MacFarland, Kim Hue-Roye, Scott Carter, Dawn Moreau, James Barry, Marilyn K. Moulds, Christine Lomas-Francis, Marion E. Reid

Immunohematology, Volume 22 , ISSUE 2, 69–71

Report | 25-March-2020

DNA-based assays for patient testing: their application, interpretation, and correlation of results

DNA analysis for the prediction of RBC phenotype has broad implication in transfusion medicine.  Hemagglutination testing, long the gold standard for immunohematology testing, has significant limitations.  DNA analysis affords a useful addition to the arsenal of methods used to resolve complex serologic investigations.  This report discusses the interpretation of results obtained by DNA analyses and their correlation with serologic results.  Some current applications to

Christine Lomas-Francis, Helene DePalma

Immunohematology, Volume 24 , ISSUE 4, 180–190

Article | 15-February-2021

An update on the Knops blood group system

J.M. Moulds

Immunohematology, Volume 35 , ISSUE 1, 16–18

Report | 24-March-2020

Transfusion service disaster planning

needs during a crisis, including the need for blood and transfusion medicine services.  The MEICS plan was developed to supplement our current local emergency preparedness procedures and provide a mechanism for responding to the escalating severity of an emergency to deal with situations of a magnitude that is outside the normal experience.  A plan was developed to interface the existing Transfusion Medicine disaster plan standard operating procedures (SOP) with the institutional and

Kevin L. Bundy, Mary L. Foss, James R. Stubbs

Immunohematology, Volume 24 , ISSUE 3, 93–101

Article | 06-December-2020

The use of computers in teaching clinical laboratory science

The Transfusion Medicine Tutor (TMT) has been designed to study the use of computers in teaching concepts and problem-solving skills important in the field of clinical laboratory science. This system provides students with an opportunity to gain experience by solving a wide range of actual cases, and coaches these students when they are having difficulties. This system is designed specifically to detect and respond to a variety of errors that students may make while solving cases, and to

T.E. Miller, P.J. Smith, S. Gross, S. Guerlain, S. Rudmann, P.L. Strohm, J.W. Smith, J.R. Svirbely

Immunohematology, Volume 9 , ISSUE 1, 22–27

Report | 25-March-2020

Principles of PCR-based assays

molecular testing, popular because they analyze multiple nucleotides in a single assay and have a high-throughput potential.  This review briefly describes the principles of PCR-based assays that are commonly used in transfusion medicine.

Kim Hue-Roye, Sunitha Vege

Immunohematology, Volume 24 , ISSUE 4, 170–175

Article | 16-November-2020

Platelet transfusion: a review of key concepts

Many clinical situations involve the platelet transfusion support of patients with thrombocytopenia. Among these are patients with leukemia, oncology patients receiving chemotherapy, patients undergoing bone marrow transplantation, patients with idiopathic thrombocytopenia purpura (ITP), and the acute trauma patient. The Transfusion Medicine service plays a key role in the care of patients in that they are expected to provide quality blood products and aid in clinical decision making. Issues

Alan J. Sheinbaum, Jay H. Herman

Immunohematology, Volume 12 , ISSUE 3, 123–126

Article | 16-February-2021

Clinical approach after identification of a rare anti-Ena in a prenatal sample

Introduction The MNS blood group system (ISBT 002) consists of red blood cell (RBC) antigens located on glycophorin A (GPA) and glycophorin B (GPB). The GYPA and GYPB genes encoding these glycophorins are located on the long arm of chromosome 4.1,2 The most frequently encountered antibodies to antigens in this system by a transfusion medicine service are those directed against M, N, S, and s. In rare cases, individuals may lack GPA or both GPA and GPB on their RBCs because of gene deletion, and

P.J. Howard, L. Guerra, D.K. Kuttner, M.R. George

Immunohematology, Volume 35 , ISSUE 4, 159–161

Article | 14-October-2020

Rh antigen and phenotype frequencies and probable genotypes for the four main ethnic groups in Port Harcourt, Nigeria

Rh is the most complex and polymorphic of the RBC group systems and is of major importance in transfusion medicine. Data are not available on the frequency of Rh antigens D, C, E, c, and e in Port Harcourt, Nigeria. Two mL of venous blood was collected into an EDTA tube from each of 400 persons of mixed ethnic groups recruited for the study. The study population comprised 167 Ijaws (41.8%), 141 Ikwerres (35.2%), 50 Ekpeyes (12.5%), and 42 Ogonis (10.5%). The RBCs were phenotyped for D, C, E, c

Zac Awortu Jeremiah, F.I. Buseri

Immunohematology, Volume 19 , ISSUE 3, 86–88

Review | 26-October-2019

Kell and Kx blood group systems

polymorphisms have not been reported. Together these two blood group systems share an integral relationship in transfusion medicine, neurology, and musculoskeletal biology.

Gregory A. Denomme

Immunohematology, Volume 31 , ISSUE 1, 14–19

Article | 01-April-2020

External quality assessment scheme in red blood cell serology: a 5-year experience in Thailand

From 2000 to 2004, 36, 58, 72, 78,and 86 laboratories participated in an external quality assessment scheme (EQAS) organized by the Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital. Each year the staff was requested to perform ABO grouping,D typing,antibody screening,antibody identification,and DATs on eight blood samples. Each participant received information on the correct test results and a coded summary. Regarding ABO grouping, the error .rate ranged from 0.3 to 1.3

Sasitorn Bejrachandra, Jariya Saipin, Oytip Nathalang, Usanee Siriboonrit, Ekaraj Rungroung, Sudjai Udee

Immunohematology, Volume 22 , ISSUE 1, 1–5

Review | 29-October-2019

Raph blood group system

cancer progression, the significance in transfusion medicine is limited to one report of a hemolytic transfusion reaction in Subject 5.

Michele Hayes

Immunohematology, Volume 30 , ISSUE 1, 6–10

Article | 30-November-2020

State association-sponsored program for SBB candidates

administration, and transfusion medicine) except infectious disease testing and management issues. The latter were presented during two free-standing one-day seminars (for which a fee was charged for those not registered for the lecture series). Educational techniques were covered in a self-study module. Two lectures were presented one morning each week from March through December, with no presentations during July. Lecturers were local volunteer experts, and detailed learning objectives were established for

W. John Judd, Louise Eisenbrey, Marty Weaver, Marliesa Back, Sharon Cisco, Janet M. Deters, Jerry M. Gray, Janis Hamilton, Mary Beth Hartrick, Miki Morrison

Immunohematology, Volume 10 , ISSUE 3, 95–98

Report | 25-March-2020

From DNA to blood groups

Marion E. Reid

Immunohematology, Volume 24 , ISSUE 4, 166–169

Case report | 16-October-2019

Management of pregnancy sensitized with anti-Inb with monocyte monolayer assay and maternal blood donation

community blood supply. Involvement of obstetric, transfusion medicine, anesthesia, and neonatology providers was imperative for a favorable outcome. The antibody did not cause clinically significant anemia in this infant.

Raj Shree, Kimberly K. Ma, Lay See Er, Meghan Delaney

Immunohematology, Volume 34 , ISSUE 1, 7–10

Article | 17-November-2020

A practice guideline and decision aid for blood transfusion

transfusions covering 628 patients were measured. In Phase 1, RBC transfusion behavior was observed without intervention. In Phase 2, a special order form for RBCs that suggested a transfusion threshold of 8.6 g/dL of Hg was introduced. In Phase 3, the suggested threshold was lowered to 7.0 g/dL and required all transfusions that did not meet the new guideline to be prospectively reviewed by a transfusion medicine physician. The Hg level at transfusion fell from 8.5 g/dL to 8.2 g/dL (p = 0.008). The use of

Benjamin Littenberg, Howard Corwin, Andrew Gettinger, Joshua Leihter, James P. AuBuchon

Immunohematology, Volume 11 , ISSUE 3, 88–94

Article | 22-January-2021

Routine indirect antiglobulin testing of blood donors—a further step toward blood safety: an experience from a tertiary care center in northern India

center at the time we established an immunohematology (IH) workup laboratory. We also report the incidence of RBC alloimmunization in healthy blood donors from a tertiary care center in northern India. Material and Methods A cross-sectional prospective study was conducted in the Department of Transfusion Medicine at a tertiary care referral and teaching institute from September 2017 to January 2019 to assess the incidence of RBC alloimmunization in healthy blood donors. The blood donors were

S. Malhotra, G. Negi, D. Kaur, S.K. Meinia, A.K. Tiwari, S. Mitra

Immunohematology, Volume 36 , ISSUE 3, 93–98

Article | 15-April-2020

Molecular characterization of GYPB and RH in donors in the American Rare Donor Program

Transfusion of patients with sickle cell disease (SCD) has been a challenge in clinical transfusion medicine, especially when the required donor RBCs must be U– and negative for high-prevalence Rh phenotypes (hrB,hrS). It is now possible to genotype donors to identify or confirm Uvar and U– phenotypes,as well as Rh hrB– and hrS– phenotypes,and to characterize the different RH backgrounds found in these donors. In a preliminary study of donors registered in the American

Sunitha Vege, Connie M. Westhoff

Immunohematology, Volume 22 , ISSUE 3, 143–147

Article | 16-February-2021

Comparison of ABO genotyping methods: a study of two low-resolution polymerase chain reaction assays in a clinical testing laboratory

J.A. Keller, T. Horn, S. Scholz, S. Koenig, M.A. Keller

Immunohematology, Volume 35 , ISSUE 4, 149–153

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