research-article | 03-August-2021
bacterial communities were screened by 16S rDNA sequencing in the four common species of jellyfish including Phyllorhiza punctata, Cyanea capillata, Chrysaora melanaster and Aurelia coerulea, to evaluate the diversity and richness as well as their potential functions involving the life of the four different jellyfish species.
Experimental
Materials and Methods
Jellyfish samples. Individuals of four jellyfish species (P. punctata, C. capillata, C. melanaster, and A. coerulea) were collected alive from
QING LIU,
XINTONG CHEN,
XIAOYA LI,
JIANPING HONG,
GUIXIAN JIANG,
HONGYU LIANG,
WENWEN LIU,
ZHENG XU,
JING ZHANG,
WEI WANG,
LIANG XIAO
Polish Journal of Microbiology, Volume 68 , ISSUE 4, 465–476
Original Paper | 30-June-2018
A bacterial isolate MR-CH-I2 [KC809939] isolated from soil contaminated mainly by high nickel concentrations in southwest Slovakia was previously found carrying nccA-like heavy-metal resistance determinant, marked as MR-CH-I2-HMR [KF218096]. According to phylogenetic analysis of short (696 bp) 16S rDNA (16S rRNA) sequences this bacterium was tentatively assigned to Uncultured beta proteobacterium clone GC0AA7ZA05PP1 [JQ913301]. nccA-like gene product was on the same base of its partial (581 bp
MATEJ REMENÁR,
ANNA KAMLÁROVÁ,
JANA HARICHOVÁ,
MARCEL ZÁMOCKÝ,
PETER FERIANC
Polish Journal of Microbiology, Volume 67 , ISSUE 2, 191–201
Original Paper | 28-June-2017
Community structure of bacteria present in arsenic contaminated agricultural soil was studied with qPCR (quantitative PCR) and DGGE (Denaturing Gradient Gel Electrophoresis) as an indicator of extreme stresses. Copy number of six common bacterial taxa (Acidobacteria, Actinobacteria, α-, β- and γ-Proteobacteria, Firmicutes) was calculated using group specific primers of 16S rDNA. It revealed that soilcontaminated with low concentration of arsenic was dominated by both
Semanti Basu,
Tanima Paul,
Priya Yadav,
Abhijit Debnath,
Keka Sarkar
Polish Journal of Microbiology, Volume 66 , ISSUE 2, 209–221
Original Paper | 26-August-2016
Wael S. El-Sayed
Polish Journal of Microbiology, Volume 65 , ISSUE 3, 341–352
original-paper | 28-March-2019
restriction analysis of the amplified product. These genes are conserved among bacteria but show small variations that allow LAB species identification (Mohania et al. 2008). Using ARDRA of 16S rDNA it is possible to differentiate the main LAB present in wine fermentation (Rodas et al. 2003), but to ensure the identification many authors have used the sequencing of the complete 16S rDNA gene (Reginensi et al. 2013). Although the sequencing of 16S rRNA genes is still considered the gold standard for
JOHANNA SÁNCHEZ,
CARLOS VEGAS,
AMPARO IRIS ZAVALETA,
BRAULIO ESTEVE-ZARZOSO
Polish Journal of Microbiology, Volume 68 , ISSUE 1, 127–137
Original Paper | 27-September-2017
Investigations of bacterial communities and characterization of mineralogy of the environment in the Złoty Stok As-Au deposit werecarried out. PXRD analysis revealed the presence of picropharmacolite as the most common secondary arsenic mineral in the mine. Total DNA was extracted from slime streams or slime biofilms samples to investigate the bacterial communities. PCR amplification of 16S rDNA was performed followed by subcloning of its products. Over 170 clones were analyzed by means of RFLP
Tomasz Cłapa,
Dorota Narożna,
Rafał Siuda,
Andrzej Borkowski,
Marek Selwet,
Cezary J. Mądrzak,
Ewa Koźlecka
Polish Journal of Microbiology, Volume 66 , ISSUE 3, 375–381
Original Paper | 28-June-2017
The purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in Enterococcus spp. isolated from different cheeses and also investigate some of their virulence factors. Enterococcus strains were isolated from 33 different cheeses. Enterococcus faecium (6 strains) and Enterococcus faecalis (5 strains) enterocin-producing strains were identified by 16S rDNA analyses. Structural genes entA, entB, entP and entX were detected in some isolates
Mine Avci,
Banu Özden Tuncer
Polish Journal of Microbiology, Volume 66 , ISSUE 2, 223–233
Original Paper | 26-August-2016
Fifty seven bacterial isolates from root nodules of two spontaneous legumes (Astragalus corrugatus and Hippocrepis areolata) growing in the arid areas of Tunisia were characterized by phenotypic features, 16S rDNA PCR-RFLP and 16S rRNA gene sequencing. Phenotypically, our results indicate that A. corrugatus and H. areolata isolates showed heterogenic responses to the different phenotypic features. All isolates were acid producers, fast growers and all of them used different compounds as sole
Mosbah Mahdhi,
Nadia Houidheg,
Neji Mahmoudi,
Abdelhakim Msaadek,
Mokhtar Rejili,
Mohamed Mars
Polish Journal of Microbiology, Volume 65 , ISSUE 3, 331–339
Original Paper | 07-June-2016
Two hundred and fifty bacterial strains were isolated from pinyon rhizosphere and screened for biosurfactants production. Among them, six bacterial strains were selected for their potential to produce biosurfactants using two low cost wastes, crude glycerol and lactoserum, as raw material. Both wastes were useful for producing biosurfactants because of their high content in fat and carbohydrates. The six strains were identified by 16S rDNA with an identity percentage higher than 95%, three
Arnoldo Wong-Villarreal,
Lizbeth Reyes-López,
Hipólito Corzo González,
Cristina Blanco González,
Gustavo Yáñez-Ocampo
Polish Journal of Microbiology, Volume 65 , ISSUE 2, 183–189
original-paper | 08-September-2020
rates in PVK liquid medium were 88 mg P/l * d and 115 mg P/l * d for bacterial strains A2 and A3, respectively. Therefore, bacterial strain A3 was selected because it was able to grow with tricalcium phosphate being the only phosphorus source on both solid and liquid media, and it solubilized orthophosphates to a higher rate than bacterial strain A2.
The strain identification based on the 16S rRNA gene sequence. The 16S rDNA sequence was analyzed using the BLASTn algorithm and showed that bacterial
GUSTAVO YAÑEZ-OCAMPO,
MARTHA E. MORA-HERRERA,
ARNOLDO WONG-VILLARREAL,
DENISSE M. DE LA PAZ-OSORIO,
NADIA DE LA PORTILLA-LÓPEZ,
JORGE LUGO,
ROCIO VACA-PAULÍN,
PEDRO DEL ÁGUILA
Polish Journal of Microbiology, Volume 69 , ISSUE 3, 357–365
Original Paper | 10-December-2018
Abstract
Paeonia ostii is known for its excellent medicinal values as Chinese traditional plant. To date, the diversity of culturable endophytes associated with P. ostii is in its initial phase of exploration. In this study, 56 endophytic bacteria and 51 endophytic fungi were isolated from P. ostii roots in China. Subsequent characterization of 56 bacterial strains by 16S rDNA gene sequence analysis revealed that nine families and 13 different genera were represented. All the fungal strains
RUI-XIAN YANG,
SHAO-WEN ZHANG,
DONG XUE,
JUN-HAO XUAN,
YUAN-BO ZHANG,
BIAO-BIAO PENG
Polish Journal of Microbiology, Volume 67 , ISSUE 4, 441–454