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  • Journal Of Nematology

 

Research Article | 26-September-2018

Occurrence of Sheraphelenchus sucus (Nematoda: Aphelenchoidinae) and Panagrellus sp. (Rhabditida: Panagrolaimidae) Associated with Decaying Pomegranate Fruit in Italy

rRNA gene, D2–D3 expansion domains of the 28S rDNA, the ITS region, and the partial mitochondrial COI were carried out. Sequences of the 18S rRNA gene, the D2–D3 domains, and the ITS were analyzed using several methods for inferring phylogeny to reconstruct the relationships among Sheraphelenchus and Bursaphelenchus species. The bacterial feeder Panagrellus sp. was characterized at the molecular level only. The D2–D3 expansion domains and ITS sequences of this Italian panagrolaimid were determined

ELENA FANELLI, ALBERTO TROCCOLI, NICOLA VOVLAS, GIANLUCA SCARCIA, ANNAMARIA MINCUZZI, SIMONA M. SANZANI, ANTONIO IPPOLITO, FRANCESCA DE LUCA

Journal of Nematology, Volume 49 , ISSUE 4, 418–426

Research Article | 03-September-2018

Nothotylenchus andrassy n. sp. (Nematoda: Anguinidae) from Northern Iran

anus distance); and elongate, conical tail with pointed tip. Nothotylenchus andrassy n. sp. is morphologically similar to five known species of the genus, namely Nothotylenchus geraerti, Nothotylenchus medians, Nothotylenchus affinis, Nothotylenchus buckleyi, and Nothotylenchus persicus. The results of molecular analysis of rRNA gene sequences, including the D2–D3 expansion region of 28S rRNA, internal transcribed spacer (ITS) rRNA and partial 18S rRNA gene are provide for the new species.

Parisa Jalalinasab, Mohsen Nassaj Hosseini, Ramin Heydari

Journal of Nematology, Volume 50 , ISSUE 2, 219–228

research-article | 24-April-2020

First report of molecular characterization and phylogeny of Trichuris fossor Hall, 1916 (Nematoda: Trichuridae)

relations among ruminant- and rodent-infecting species (Doležalová et al., 2015). However, the number of variants of RNA genes (including the ITS2 region) makes their utility in disentangling the phylogeny of Trichuris less opportune, particularly given that the amount of ploidy is unknown (Doležalová et al., 2015). The 18S rRNA gene has been used to infer the placement of trichurids within Nematoda as well as to elucidate relationships within Trichuridae and is less prone to result in unclear multiple

Malorri R. Hughes, Deborah A. Duffield, Dana K. Howe, Dee R. Denver

Journal of Nematology, Volume 52 , 1–6

research-article | 30-November-2020

Morphological and molecular characterization of Xiphinemella esseri Chitwood, 1957 (Dorylaimida: Leptonchidae) from Florida, with the first molecular study of the genus

D3B (5′-TCG GAA GGA ACC AGC TAC TA-3′) (Subbotin et al., 2006). The 18S rRNA gene was amplified using two sets of primers (two overlapping fragments): (i) forward G18SU (5′-GCT TGT CTC AAA GAT TAA GCC-3′) and reverse R18Tyl1 (5′-GGT CCA AGA ATT TCA CCT CTC-3′) and (ii) forward F18Tyl2 (5′-CAG CCG CGG TAA TTC CAG C-3′) and reverse R18Tyl2 (5′-CGG TGT GTA CAA AGG GCA GG-3′)(Chizhov et al., 2006). PCR products were purified using the QIAquick PCR purification Kit (Qiagen) and used for direct

Sergio Álvarez-Ortega, Sergei A. Subbotin, Renato N. Inserra

Journal of Nematology, Volume 53 , 1–9

research-article | 25-May-2020

Description and molecular phylogeny of Mesocriconema abolafiai n. sp. (Nematoda: Criconematidae) from Iran

For DNA amplification the protocol described by Tanha Maafi et al. (2003) was used. The D2 to D3 expansion regions of the 28S rRNA gene was amplified with the forward D2A (5´-ACAAGTACCGTGAGGGAAAGTTG-3´) and the reverse D3B (5´-TCGGAAGGAACCAGCTACTA-3´) primers (Nunn, 1992). The 18S rRNA was amplified as two partially overlapping fragments, using three universal and one nematode-specific primer (1912R). First 18S fragment forward primer 988F (5´-CTCAAAGATTAAGCCATGC-3´) and reverse primer 1912R (5

Hossein Mirbabaei Karani, Ali Eskandari, Reza Ghaderi, Akbar Karegar

Journal of Nematology, Volume 52 , 1–17

Article | 21-July-2017

Morphological and Molecular Characterization of Two Aphelenchoides Endophytic in Poplar Leaves

northwestern North America were discovered. Nematodes were identified and characterized microscopically and molecularly with 28S ribosomal RNA (rRNA) and 18S rRNA molecular markers. From P. angustifolia, Aphelenchoides saprophilus was inferred to be closest to another population of A. saprophilus among sequenced taxa in the 18S tree. From P. trichocarpa, Laimaphelenchus heidelbergi had a 28S sequence only 1 bp different from that of a Portuguese population, and 1 bp different from the

LYNN K. CARTA, SHIGUANG LI, ANDREA M. SKANTAR, GEORGE NEWCOMBE

Journal of Nematology, Volume 48 , ISSUE 1, 28–33

research-article | 24-April-2020

Mitochondrial COI gene is valid to delimitate Tylenchidae (Nematoda: Tylenchomorpha) species

glass slide. The nematode cuticle was broken by a needle and subsequently transferred to a 200 μl Eppendorf tube. After 1 min for freezing in liquid nitrogen, 1 μl proteinase K (1.0 mg/ml) was added and incubated for 1 h at 65˚C and 10 min at 95˚C. The 18S rRNA was amplified with primers 1096F (5´-GGT AAT TCT GGA GCT AAT AC-3´), 988F (5´-CTC AAA GAT TAA GCC ATG C-3´), 1912R (5´-TTT ACG GTC AGA ACT AGG G-3´), 1813F (5´-CTG CGT GAG AGG TGA AAT-3´), and 2646R (5´-GCT ACC TTG TTA CGA CTT TT-3

Mengxin Bai, Xue Qing, Kaikai Qiao, Xulan Ning, Shun Xiao, Xi Cheng, Guokun Liu

Journal of Nematology, Volume 52 , 1–12

Research Article | 17-October-2018

First Report of Stubby-Root Nematode, Paratrichodorus minor, on Onion in Georgia, U.S.A

of females (n = 20) included: body length 671.1 (570.1–785.3) µm; body width 32.5 (27.8–37.0) µm; onchiostyle 32.5 (31.1–34.8) µm; anterior end to esophagus-intestinal valve 117.6 (101.2–128.5) µm; a 21.5 (15.3–28.1) µm; b 5.2 (4.9–6.3) µm; V 52.9% (48.1–55.4%) µm; and vagina length 8.7 (7.8–10.7) µm. To confirm the identity of P. minor, DNA was extracted from single females (n = 3) using Extract-N-Amp™ Tissue PCR Kit (Sigma-Alredich Inc., St. Louis, MO). The partial 18S rRNA, the D2-D3 expansion

Abolfazl Hajihassani, Negin Hamidi, Bhabesh Dutta, Chris Tyson

Journal of Nematology, Volume 50 , ISSUE 3, 453–455

research-article | 30-November-2019

Description of Deladenus gilanica n. sp. (Hexatylina: Neotylenchidae) isolated from wood of black pine in Northern Iran

infective females. Molecular phylogeny and discussion Amplification of the partial 18S and 28S D2/D3 rRNA gene sequences from D. gilanica n. sp. specimens yielded single fragments of approximately 900 and 800 bp, respectively, based on gel size. The partial 18S rRNA gene sequence of D. gilanica n. sp. (GenBank Accession No. MF043926) was less than 96% homologous to any available DNA sequences from GenBank. The BlastN search revealed the highest match with sequences of a member of the Neotylenchidae

Parisa Jalalinasab, Mehrab Esmaeili, Weimin Ye, Ramin Heydari

Journal of Nematology, Volume 52 , 1–10

research-article | 30-November-2020

Steinernema sandneri n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Poland

Magdalena Lis, Ewa Sajnaga, Marcin Skowronek, Adrian Wiater, Kamila Rachwał, Waldemar Kazimierczak

Journal of Nematology, Volume 53 , 1–24

original-paper | 05-August-2020

Molecular Identification and Prevalence of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in Erbil City, Northern Iraq

column with a low salt buffer. DNA concentration was measured with a nanospectrophotometer; then, each sample was labeled and stored at –20°C. A nested PCR was performed. The first PCR targeted the Entamoeba genus by amplifying the 897 bp of the 18S rRNA gene, while the second PCR primers targeted E. histolytica, E. dispar, and E. moshkovskii by amplifying the 439 bp, 174 bp, and 553 bp respectively. This method was previously described by Khairnar and Parija (2007). The primers targeting the 18S

SHLER AKRAM FAQE MAHMOOD, HAWRI MUSTAFA BAKR

Polish Journal of Microbiology, Volume 69 , ISSUE 3, 263–272

research-article | 30-November-2018

Meloidogyne aegracyperi n. sp. (Nematoda: Meloidogynidae), a root-knot nematode parasitizing yellow and purple nutsedge in New Mexico

and samples were well mixed then stored at −20°C prior to PCR. Sequence-based identification of individual nematodes was performed using 18S rRNA and heat shock protein 90 (Hsp90) markers with primers used for amplification and sequencing listed in Table 1. Amplification of the 18S rRNA was performed using two primer sets; 79 F deg plus 1629 R deg which amplifies the majority of the 18S rRNA gene and 983 F deg plus Nema 28S R AG which amplifies the 3′ ~1/2 of the 18S rRNA gene and the ITS region

J. D. Eisenback, L. A. Holland, J. Schroeder, S. H. Thomas, J. M. Beacham, S. F. Hanson, V. S. Paes-Takahashi, P. Vieira

journal of nematology, Volume 51 , 1–16

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