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research-article | 30-November-2019

Potential of entomopathogenic nematodes against the pupal stage of the apple maggot Rhagoletis pomonella (Walsh) (Diptera: Tephritidae)

. Short-term exposure bioassay This experiment as well as the others described below were organized as completely randomized designs (CRD) and conducted at USDA-ARS research station in Byron, GA. Bioassay procedures were based on prior EPN virulence assays (Shapiro-Ilan et al., 2002). The experimental arena consisted of a lidded 30 ml plastic cup filled with 10 g of autoclaved sand with 0% soil moisture content. Each cup contained one pupa (recently pupated) at the bottom of the cup underneath the

Muhammad Usman, Sehrish Gulzar, Waqas Wakil, Jaime C. Piñero, Tracy C. Leskey, Laura J. Nixon, Camila Oliveira-Hofman, Shaohui Wu, David Shapiro-Ilan

Journal of Nematology, Volume 52 , 1–9

research-article | 19-March-2020

Effect of an Alltech soil health product on entomopathogenic nematodes, root-knot nematodes and on the growth of tomato plants in the greenhouse

were used for experimentation no more than two weeks post emergence. EPN bioassay to determine lethal and sub-lethal concentrations of ACS 5075, Alltech® All the treatment studies were conducted in 96-well plates. Freshly cultured EPN, not older than two weeks post emergence, were used for the study. EPN were kept in room temperature for 30 min before conducting the experiments to check their viability. The four EPN strains were concentrated by transferring the cultures into sterile and clean

Anusha Pulavarty, Karina Horgan, Thomais Kakouli-Duarte

Journal of Nematology, Volume 52 , 1–10

research-article | 06-March-2020

Control of the tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) larvae in laboratory using entomopathogenic nematodes from subtropical environment

157-C Citrus orchard Friedenheim, Mpumalanga EU625295 685 (570–740) 29 (24–33) Malan et al. (2011) S. jeffreyense J194 Guava tree Jeffrey’s Bay, Eastern Cape KC897093 924 (784–1043) 35 (23–43) Malan et al. (2016) Laboratory bioassay Virulence experiments were conducted in 24-well bioassay plates (flat-bottom, Nunce, Cat. No.144530, Thermo Fisher Scientific (Pty) Ltd, Gauteng, Johannesburg, South Africa). The 24-well bioassay protocol was used to test the potential of Steinernema

Bonginkhosi E. Dlamini, Nelisiwe Dlamini, Michael T. Masarirambi, Nxumalo Kwanele A.

Journal of Nematology, Volume 52 , 1–8

Article | 05-December-2017

Vertical Distribution of Pasteuria penetrans Parasitizing Meloidogyne incognita on Pittosporum tobira in Florida

RICHARD BAIDOO, TESFAMARIAM MEKETE MENGISTU, JANETE A. BRITO, ROBERT MCSORLEY, ROBERT H. STAMPS, WILLIAM T. CROW

Journal of Nematology, Volume 49 , ISSUE 3, 311–315

Research Article | 03-December-2018

A novel in vitro chemotaxis bioassay to assess the response of Meloidogyne incognita towards various test compounds

Tagginahalli N. Shivakumara, Tushar K. Dutta, Uma Rao

Journal of Nematology, Volume 50 , ISSUE 4, 487–494

Research Article | 03-December-2018

Nematotoxic coumarins from Angelica pubescens Maxim. f. biserrata Shan et Yuan roots and their physiological effects on Bursaphelenchus xylophilus

The ethanol extracts from the roots of Angelica pubescens Maxim. f. biserrata Shan et Yuan was toxic against the pine wood nematode Bursaphelenchus xylophilus. The ethyl acetate-soluble fraction derived from this extract increased its potency with a mortality of 95.25% in 72 hr at 1.0 mg/mL. Four nematotoxic coumarins were obtained from the ethyl acetate extract by bioassay-guided isolation. These were identified as osthole 1, columbianadin 2, bergapten 3 and xanthotoxin 4 by mass and nuclear

Qun-Qun Guo, Gui-Cai Du, Yong-Xing Li, Chen-Yan Liang, Chao Wang, Ya-Nan Zhang, Rong-Gui Li

Journal of Nematology, Volume 50 , ISSUE 4, 559–568

research-article | 30-November-2018

Movement of seed- and soil-applied fluopyram in soil columns

were drenched with 0.12 µl water/mm3 soil at a base rate of water infiltration of 20.0 mm/d on day two and incubated overnight in a resealable plastic bag. Plastic tubes were cut into 5-cm-long segments on day three and the soil within each segment dislodged into a 5-ml centrifuge tube (Eppendorf Ag, Hamburg, Germany) that contained 1 ml sterilized distilled water and vortexed for 30 sec. The supernatant was used immediately in a nematode motility bioassay. These bioassays were performed in 24-well

Travis R. Faske, Katherine Brown

Journal of Nematology, Volume 51 , 1–8

research-article | 30-November-2019

Evaluation of fluopyram for the control of Ditylenchus dipsaci in sugar beet

performed with R. A logistic dose-response curve was used in the motility bioassay to describe the relationship between inactive D. dipsaci after 24, 48, 72, 96 h, and 72+rinse (y) and the concentration (x) of the active ingredient fluopyram (Hutchinson et al., 1999; Kiewnick and Sikora, 2006). The logistic curve can be expressed as follows: (1) y = c + [ d − c 1 + ( x EC 50 ) ] b , (1)where c and d are the lower and upper limits of the curve, respectively. The EC50 is the

Alan Storelli, Andreas Keiser, Reinhard Eder, Samuel Jenni, Sebastian Kiewnick

Journal of Nematology, Volume 52 , 1–10

Original Paper | 30-March-2017

Suppressive Effect of Trichoderma spp. on toxigenic Fusarium species

cerealis, Fusarium culmorum, Fusarium graminearum and Fusarium temperatum). Dual-culture bioassay on potato dextrose agar (PDA) medium clearly documented that all of the Trichoderma strains used in the study were capable of influencing the mycelial growth of at least four of all five Fusarium species on the fourth day after co-inoculation, when there was the first apparent physical contact between antagonist and pathogen. The qualitative evalu­ation of the interaction between the colonies after 14

Lidia Błaszczyk, Aneta Basińska-Barczak, Hanna Ćwiek-Kupczyńska, Karolina Gromadzka, Delfina Popiel, Łukasz Stępień

Polish Journal of Microbiology, Volume 66 , ISSUE 1, 85–100

Research Article | 26-September-2018

Control of Globodera spp. Using Brassica juncea Seed Meal and Seed Meal Extract

traditional B. juncea seed meal, as alternate eradication strategies. This is the first report on the efficacy of B. juncea seed meal extract against plant-parasitic nematodes. Rates of B. juncea seed meal greater than 2.2 t/ha and 4.5 t/ha for G. pallida and G. ellingtonae, respectively, were required for egg hatch suppression, as determined by a potato root diffusate (PRD) bioassay. Reproduction of G. pallida on potato after exposure to B. juncea seed meal at a rate of 2.2 t/ha was also significantly

LOUISE-MARIE DANDURAND, MATT J. MORRA, INGA A. ZASADA, WENDY S. PHILLIPS, INNA POPOVA, COLE HARDER

Journal of Nematology, Volume 49 , ISSUE 4, 437–445

research-article | 30-November-2020

The impact of chemical nematicides on entomopathogenic nematode survival and infectivity

above. There were 12 replicates, and the experiment was conducted twice on different dates. Virulence, penetration efficiency, and reproduction of EPNs IJ infectivity was assessed against G. mellonella using a sand-well bioassay in 24-well plates (Touray et al., 2020). During the assessment of IJ survival, 50 live IJs exposed to a given nematicide for 24 and 48 h were collected from each treatment and rinsed with water and then were transferred with 60 μl distilled water into wells containing 0.5

Mustapha Touray, Harun Cimen, Sebnem H. Gulsen, Derya Ulug, Dolunay Erdogus, David Shapiro-Ilan, Selcuk Hazir

Journal of Nematology, Volume 53 , 1–17

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