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  • Journal Of Nematology

 

research-article | 30-November-2020

Cultivation of Caenorhabditis elegans on new cheap monoxenic media without peptone

may have included additional nutrients. Brood size assays Prior to the brood assays, L1 stage worms growing on NGM were transferred onto the test media to allow acclimation for one generation. From their progeny, a batch of 2 to 10 (mode = 7) synchronized L2 hermaphrodites (P0) were placed onto each bacterium-seeded test media plate. The P0s were transferred to a new plate every day until they stopped laying eggs. Eggs on each plate were allowed to hatch and develop until the L2 to L4 stages

Tho Son Le, T. T. Hang Nguyen, Bui Thi Mai Huong, H. Gam Nguyen, B. Hong Ha, Van Sang Nguyen, Minh Hung Nguyen, Huy-Hoang Nguyen, John Wang

Journal of Nematology, Volume 53 , 1–23

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