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  • Journal Of Nematology

 

research-article | 30-November-2020

First report of Cactodera milleri Graney and Bird, 1990 from Colorado and Minnesota

county, Colorado, USA. A, B: J2 anterior ends; C, D: J2 tails; E: J2 lateral field; F: Cyst posterior (ventral view); G: Cyst cone mount. Representative J2 from two CO isolates and three MN isolates were used for molecular confirmation of the species, using two ribosomal genes (internal transcribed spacer, ITS 1 and 2 and large ribosomal subunit, 28S), one nuclear gene (partial heat shock protein 90, Hsp90), and one mitochondrial gene (partial cytochrome oxidase I, COI). Markers were amplified

Andrea M. Skantar, Zafar A. Handoo, Mihail R. Kantor, Saad L. Hafez, Maria N. Hult, Kathryn Kromroy, Kimberly Sigurdson, Michelle Grabowski

Journal of Nematology, Volume 53 , 1–7

research-article | 24-April-2020

Mitochondrial COI gene is valid to delimitate Tylenchidae (Nematoda: Tylenchomorpha) species

et al., 2006; Subbotin et al., 2006). However, rRNA genes are problematic in Tylenchidae phylogeny and the unresolved status is unlikely to be improved by intensive species sampling (Qing et al., 2017; Qing and Bert, 2019). Therefore, finding a proper molecular marker gene is crucial for the Tylenchidae study. In this study we examined the mitochondrial Cytochrome Oxidase I gene (COI) of 12 species belong to Tylenchidae (sensu (Geraert, 2008)), the goal is to evaluate the potential of COI

Mengxin Bai, Xue Qing, Kaikai Qiao, Xulan Ning, Shun Xiao, Xi Cheng, Guokun Liu

Journal of Nematology, Volume 52 , 1–12

research-article | 30-November-2020

Genetic variation within a species of parasitic nematode, Skrjabingylus chitwoodorum, in skunks

oxidase I gene for 44 samples of Skrjabingylus. Maximum likelihood analysis was performed using the best-fitting model, Hasegawa-Kishino-Yano of DNA substitution with Gamma distribution. Bootstrap values are based on 1000 replicates and values ≥70 are shown on branches. Number with prefix ASK identifies the specific host from which the sample was collected. Prefix KP is a Genbank accession number. Within S. chitwoodorum samples, there was no obvious pattern of close geographic relationships other

Allie N. Denham, Malorri R. Hughes, Robert C. Dowler, Nicholas J. Negovetich, Loren K. Ammerman

Journal of Nematology, Volume 53 , 1–8

research-article | 30-March-2020

Characterization of Vittatidera zeaphila (Nematoda: Heteroderidae) from Indiana with molecular phylogenetic analysis of the genus

 μl 50 mM MgCl2, and 2.5 µl PCR buffer in a total volume of 25 µl. PCR cycling conditions were 95°C for 3 min, 35X (94°C for 30 sec, 50°C for 40 sec, 72°C for 70 sec), 72°C for 5 min, 4°C until finish. COI: Mitochondrial cytochrome oxidase I (COI) was amplified with primers Het-coxiF [5′-TAGTTGATCGTAATTTTAATGG-3′] and Het-coxiR [5′-CCTAAAACATAATGAAAATGWGC-3′]. Amplifications were performed in 25 µl reactions with 1x PicoMaxx (Agilent) buffer, 0.2 mM dNTPs, 0.3 µM each primer, 0.125 µl Dream Taq

Andrea M. Skantar, Zafar A. Handoo, Mihail R. Kantor, Lynn K. Carta, Jamal Faghihi, Virginia Ferris

Journal of Nematology, Volume 52 , 1–8

research-article | 30-November-2019

Morphological and molecular characterization of Pratylenchus species from Yam (Dioscorea spp.) in West Africa

Yao A. Kolombia, Oluwadamilola Ogundero, Emmanuel Olajide, Nicole Viaene, P. Lava Kumar, Danny L. Coyne, Wim Bert

Journal of Nematology, Volume 52 , 1–25

Research Article | 17-October-2018

First Report of the Yellow Nutsedge Cyst Nematode, Heterodera cyperi, in Georgia, U.S.A.

extracted from single cysts (n = 3) and internal transcribed spacer (ITS) of rRNA and partial cytochrome oxidase I (COI) genes were amplified with primers TW81/AB28 and Het-coxiF/Het-coxiR, respectively (Subbotin et al., 2001; Subbotin, 2015) and sequenced. The resulting sequences were deposited into the GenBank database (Accession no. MG825344 and MG857126) and also subjected to BLAST searches in the database. ITS sequence of H. cyperi showed 100% similarity (100% coverage) with that of a H. cyperi

Abolfazl Hajihassani, Bhabesh Dutta, Ganpati B. Jagdale, Sergei A. Subbotin

Journal of Nematology, Volume 50 , ISSUE 3, 456–458

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