research-article | 30-November-2018
) and observed under a scanning electron microscope (Jeol JSM-6390) at 10 kV.
Molecular study
For molecular analysis, DNA was extracted with DNeasy Blood and Tissue Kit (Qiagen Inc., Valencia, CA). Two rRNA fragments, i.e. the LSU D2–D3 and ITS regions, were amplified. Primers for D2–D3 segments amplification were D2A (5′-ACAAGTACCGTGAGGGAAAGTTG-3′) and D3B (5′-TCGGAAGGAACCAGCTACTA-3′). Primers for ITS amplification were TW81 (5′-GTTTCCGTAGGTGAACCTGC-3′) and AB28 (5′-ATATGCTTAAGTTCAGCGGGT-3
Heonil Kang,
Jongmin Seo,
Garam Han,
Donggeun Kim,
Insoo Choi
Journal of Nematology, Volume 51 , 1–12