• Select Article Type
  • Abstract Supplements
  • Blood Group Review
  • Call to Arms
  • Communications
  • Hypothesis
  • In Memoriam
  • Interview
  • Introduction
  • Letter to the Editor
  • Short Report
  • abstract
  • Abstracts
  • Article
  • book-review
  • case-report
  • case-study
  • Clinical Practice
  • Commentary
  • Conference Presentation
  • conference-report
  • congress-report
  • Correction
  • critical-appraisal
  • Editorial
  • Editorial Comment
  • Erratum
  • Events
  • in-memomoriam
  • Letter
  • Letter to Editor
  • mini-review
  • minireview
  • News
  • non-scientific
  • Obituary
  • original-paper
  • original-report
  • Original Research
  • Pictorial Review
  • Position Paper
  • Practice Report
  • Preface
  • Preliminary report
  • Product Review
  • rapid-communication
  • Report
  • research-article
  • Research Communicate
  • research-paper
  • Research Report
  • Review
  • review -article
  • review-article
  • review-paper
  • Review Paper
  • Sampling Methods
  • Scientific Commentary
  • serologic-method-review
  • short-communication
  • short-report
  • Student Essay
  • Varia
  • Welome
  • Select Journal
  • Polish Journal Of Microbiology
  • Journal Of Nematology
  • Immunohematology


original-paper | 18-February-2020

Assessing the Microbial Communities in Four Different Daqus by Using PCR-DGGE, PLFA, and Biolog Analyses

rise to distinctive flavors. In the past, culture-dependent methods were the commonest approach to microbial profiling analysis. However, microorganisms identified using these methods amount to ≤ 10% of the total environmental microorganisms and do not reflect the actual microbial profile distribution within Daqu (Liu et al. 2017). Culture-independent methods such as polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis are highly useful to detect the whole microbial


Polish Journal of Microbiology, Volume 69 , ISSUE 1, 27–37

Original Paper | 28-June-2017

Molecular Study of Indigenous Bacterial Community Composition on Exposure to Soil Arsenic Concentration Gradient

Community structure of bacteria present in arsenic contaminated agricultural soil was studied with qPCR (quantitative PCR) and DGGE (Denaturing Gradient Gel Electrophoresis) as an indicator of extreme stresses. Copy number of six common bacterial taxa (Acidobacteria, Actinobacteria, α-, β- and γ-Proteobacteria, Firmicutes) was calculated using group specific primers of 16S rDNA. It revealed that soilcontaminated with low concentration of arsenic was dominated by both

Semanti Basu, Tanima Paul, Priya Yadav, Abhijit Debnath, Keka Sarkar

Polish Journal of Microbiology, Volume 66 , ISSUE 2, 209–221

Research Article | 03-December-2018

Effector gene vap1 based DGGE fingerprinting to assess variation within and among Heterodera schachtii populations

, and sequence this gene from diverse species and populations of cyst nematodes. This resulted in a high diversity of sequences for H. schachtii, and allowed to design non-degenerated primers to amplify a fragment suitable for sequence dependent separation of gene variants in denaturing gradient gel electrophoresis (DGGE). The developed primers span a highly variable intron and part of a slightly variable exon. A marker comprised of the 14 mostly detected gene variants was established for gel-to-gel

Rasha Haj Nuaima, Johannes Roeb, Johannes Hallmann, Matthias Daub, Sandra Otte, Holger Heuer

Journal of Nematology, Volume 50 , ISSUE 4, 517–528

Original Paper | 26-August-2016

Characterization of a Highly Enriched Microbial Consortium Reductively Dechlorinating 2,3-Dichlorophenol and 2,4,6-Trichlorophenol and the Corresponding cprA Genes from River Sediment

acceptors. When acetate, formate, or pyru­vate were used as electron donors, dechlorination activity was lost. Only lactate can replace dihydrogen as an electron donor. However, the dechlorination potential was decreased after successive transfers. To reveal chlororespiring species, the microbial community structure of chlorophenol-reductive dechlorinating enrichment cultures was analyzed by PCR-denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments. Eight dominant bacteria were

Wael S. El-Sayed

Polish Journal of Microbiology, Volume 65 , ISSUE 3, 341–352

Original Paper | 09-March-2018

Natural Attenuation Potential of Polychlorinated Biphenyl-Polluted Marine Sediments

petroleum hydrocarbons (TPHs). Then we adopted both culture-dependent and culture-independent (PCR-DGGE) approaches to identify bacterial inhabitants of the polluted marine sediments from Shuwaikh harbor. The chemical analysis revealed spatial variation among the sampling stations in terms of total amount of PCBs, TPHs and the PCB congener fingerprints. Moreover, in all analyzed sediments, the medium-chlorine PCB congeners were more abundant than the low-chlorine and high-chlorine counterparts. PCR-DGGE

Sarah Aldhafiri, Huda Mahmoud, Mohammed Al-Sarawi, Wael A. Ismail

Polish Journal of Microbiology, Volume 67 , ISSUE 1, 37–48

Article | 16-November-2020

Rhmod phenotype: a parentage problem solved by denaturing gradient gel electrophoresis of genomic DNA

support this hypothesis, DNA analysis of the RHD and RHCE genes was performed on the five family members. Polymerase chain reaction (PCR) products from exons 2 and 5 were analyzed by denaturing gradient gel electrophoresis (DGGE). The DNA results corroborated the serologic findings and refuted the exclusion of paternity.

Fiona J. Steers, Maura Wallace, Marialuisa Mora, Ben Carritt, Patricia Tippett, Geoff Daniels

Immunohematology, Volume 12 , ISSUE 4, 154–158

No Record Found..
Page Actions