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Article | 24-July-2017

Introduction to Nematode Genome and Transcriptome Announcements in the Journal of Nematology

The Journal of Nematology now offers publication of Nematode Genome Announcements (NGA) and Nematode Transcriptome Announcements (NTA). These brief reports announce the sequencing and assembly of a nematode genome or transcriptome resource, along with basic technical information on DNA sequencing and bioinformatic methods used. This publishing initiative offers a new avenue to openly and concisely communicate the availability and relevance of genome and transcriptome sequence resources to the

DEE R. DENVER, ERIK J. RAGSDALE, W. KELLEY THOMAS, INGA A. ZASADA

Journal of Nematology, Volume 49 , ISSUE 2, 125–126

original-paper | 14-May-2021

Genomic Analysis of the Mycoparasite Pestalotiopsis sp. PG52

DENGYUN ZHANG, JINDE YU, CHANGLE MA, LEI KONG, CHENGZHONG HE, JING LI

Polish Journal of Microbiology, Volume 70 , ISSUE 2, 189–199

Research Article | 24-July-2017

The Draft Genome of Globodera ellingtonae

Globodera ellingtonae is a newly described potato cyst nematode (PCN) found in Idaho, Oregon, and Argentina. Here, we present a genome assembly for G. ellingtonae, a relative of the quarantine nematodes G. pallida and G. rostochiensis, produced using data from Illumina and Pacific Biosciences DNA sequencing technologies.

WENDY S. PHILLIPS, DANA K. HOWE, AMANDA M. V. BROWN, SEBASTIAN EVES-VAN DEN AKKER, LEVI DETTWYLER, AMY B. PEETZ, DEE R. DENVER, INGA A. ZASADA

Journal of Nematology, Volume 49 , ISSUE 2, 127–128

research-article | 30-November-2019

Aphelenchus yinyuensis n. sp. (Tylenchina: Aphelenchoididae) found in Terminalia sp. in China

Gu Jianfeng, Munawar Maria, Yiwu Fang, Liu Lele, Xianfeng Chen, Bo Cai

Journal of Nematology, Volume 52 , 1–12

research-article | 30-November-2018

Description of Ektaphelenchus koreanus n. sp. (Nematoda: Ektaphelenchidae) with morphometrical notes on the Ektaphelenchus species

Jianfeng Gu, Munawar Maria, Yiwu Fang, Lele Liu

Journal of Nematology, Volume 51 , 1–13

Research Article | 03-December-2018

Description of Gracilacus paralatescens n. sp. (Nematoda:Paratylenchinae) found from the rhizosphere of Bamboo in Zhejiang, China

Munawar Maria, Ruihang Cai, Weimin Ye, Thomas O. Powers, Jingwu Zheng

Journal of Nematology, Volume 50 , ISSUE 4, 611–622

Research Article | 31-May-2018

Incidence of Oscheius onirici (Nematoda: Rhabditidae), a potentially entomopathogenic nematode from the marshlands of Wisconsin, USA

Weimin Ye, Shane Foye, Ann E. MacGuidwin, Shawn Steffan

Journal of Nematology, Volume 50 , ISSUE 1, 9–26

research-article | 30-November-2018

Updated description of Paratylenchus lepidus Raski 1975 and P. minor Sharma, Sharma and Khan, 1986 by integrating molecular and ultra-structural observations

Munawar Maria, Wentao Miao, Weimin Ye, Jingwu Zheng

journal of nematology, Volume 51 , 1–13

research-article | 18-March-2020

A new rare nematode Nothocriconemoides hangzhouensis n. sp. (Nematoda: Criconematidae) from Hangzhou, China

Munawar Maria, Wentao Miao, Ruihang Cai, Pablo Castillo, Jingwu Zheng

Journal of Nematology, Volume 52 , 1–14

research-article | 16-April-2020

A new stunt nematode, Geocenamus chengi n. sp. (Nematoda: Merliniinae) in the rhizosphere of tea (Camellia sinensis) from Zhejiang Province, China

Munawar Maria, Wentao Miao, Pablo Castillo, Jingwu Zheng

Journal of Nematology, Volume 52 , 1–13

Research Article | 23-May-2019

IMPLEMENTATION OF WHOLE GENOME SEQUENCING FOR BACTERIA GENOTYPING

The molecular typing methods are used to identify specific genetic targets and relationships between microbial isolates. In order to understand clonal relatedness between the microbial strains, classic phenotypic methods are used in line with modern molecular biology techniques. The development of genetics, especially new techniques like molecular typing, have revolutionized microbial research. After 1970, the development techniques, especially those referring to DNA sequencing, established

Daria Artyszuk, Tomasz Wołkowicz

Postępy Mikrobiologii - Advancements of Microbiology, Volume 57 , ISSUE 2, 179–193

original-paper | 08-September-2020

A Polyclonal Spread Emerged: Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from the Intensive Care Unit in a Chinese Tertiary Hospital

). The genes encoding carbapenemases were investigated by polymerase chain reaction (PCR) using a series of primers as previously reported (Queenan and Bush 2007; Nordmann et al. 2011). The amplification products were sent for DNA sequencing (Qingke Biotech, Hangzhou). Isolates genotyping. CRKP isolates in the study were genotyped by multilocus sequence typing (MLST). Seven housekeeping genes including gapA, infB, mdh, pgi, phoE, rpoB, and tonB of K. pneumoniae were amplified and sequenced based on

ZHENGZHENG WANG, FANGYOU YU, XIAOFEI SHEN, MEILAN LI

Polish Journal of Microbiology, Volume 69 , ISSUE 3, 311–319

Report | 26-October-2019

High-resolution melting analysis as an alternative method for human neutrophil antigen genotyping

genomic DNA samples were amplified via PCR with HNA-specific primers. Nucleotide substitutions in genes encoding HNAs were differentiated on the basis of the HRM curves, and the results of HRM and DNA sequencing analyses were determined to be in complete agreement. The gene frequency of HNA-1a, -1b, -1c, -3a, -3b, -4a, -4b, -5a, and -5b in the Japanese population was consistent with the previous reports. Our results suggest that HRM analysis can be used for genotyping HNA antigens determined by single

Kazuta Yasui, Mitsunobu Tanaka, Tomoya Hayashi, Nobuki Matsuyama, Ayumu Kuroishi, Rika. A. Furuta, Yoshihiko Tani, Fumiya Hirayama

Immunohematology, Volume 31 , ISSUE 1, 7–13

Article | 01-April-2020

A single base insertion of the 4-α-galactosyltransferase gene led to the deficiency of Gb3 biosynthesis

cDNAs for α 1,4 galactosyltransferase (A4GALT) have been isolated. To explore the molecular basis of the p phenotype in Japanese donors, we analyzed the A4GALT gene sequences of normal and p phenotype samples. The coding region in the A4GALT gene for DNA sequencing was amplified by PCR amplification. A4GALT expression vectors for individual mutants were constructed by PCR amplification of the coding region using primers and subsequent subcloning into an expression vector. The expression

Mitsunobu Tanaka, Naoko Yamashita, Junko Takahashi, Fumiya Hirayama, Yoshihiko Tani, Hirotoshi Shibata

Immunohematology, Volume 22 , ISSUE 1, 23–29

Original Paper | 04-December-2017

Characterization of Bacteriocin-Producing Lactic Acid Bacteria Isolated from Native Fruits of Ecuadorian Amazon

method the active synthetized components released in the neu­tralized and hydroxide peroxide eliminated cell-free supernatant were inhibited by proteolytic enzymes, while the activity was maintained stable after the treatment with catalase, lysozyme, α-amylase and lipase suggesting their proteinaceous nature. The inhibitory activity was stimulated by acidic conditions, upon exposure to high heat and maintained stable at different ranges of sodium chloride (4–10%). The DNA sequencing

Karina Garzón, Clara Ortega, Gabriela N. Tenea

Polish Journal of Microbiology, Volume 66 , ISSUE 4, 473–481

original-paper | 11-March-2020

Oligotrophic Nitrification and Denitrification Bacterial Communities in a Constructed Sewage Treatment Ecosystem and Nitrogen Removal of Delftia tsuruhatensis NF4

known from their culturable microorganisms. We know that traditional pure-culture methods can identify only a few microorganisms (Ward et al. 1990). Recently, the culture-independent molecular biological methods, such as Illumina HiSeq DNA sequencing has been used besides conventional isolation techniques for the analysis of microbial community structures (Watson et al. 2014). So far, there has been no report on the bacterial community in STE using both the Illumina HiSeq DNA sequencing and

RUILAN YANG, JING LI, LUYAO WEI-XIE, LIN SHAO

Polish Journal of Microbiology, Volume 69 , ISSUE 1, 99–108

Article | 18-October-2020

Fyx is associated with two missense point mutations in its gene and can be detected by PCR–SSP

;) samples of Tanzanian origin were correctly typed and of 300 random donors of Caucasian origin with known Fy phenotype, only four out of 59 Fy(a+b–) donors showed the discrepant DNA-type Fy(a+b+). Serologic reinvestigation by adsorption and elution techniques confirmed weakly expressed Fyb antigen in these cases and DNA sequencing of the entire Duffy gene revealed identical point mutations in all of them. Specific PCR reactions were used to reinvestigate the C265T (Arg89Cys) and G298A (Ala100Thr

Christoph Gassner, Richard L. Kraus, Tadeja Dovc, Susanne Kilga-Nogler, Irene Utz, Thomas Mueller, Friedrich Schunter, Diether Schoenitzer

Immunohematology, Volume 16 , ISSUE 2, 61–67

original-paper | 03-September-2019

Prevalence and Antifungal Susceptibility of the Emerging Fungal Species, Candida nivariensis, Isolated in a Teaching Hospital in Poland

MAGDALENA SIKORA, ROBERT KUTHAN, KATARZYNA PISKORSKA-MALOLEPSZA, MARLENA GOLAS-PRADZYNSKA, DARIUSZ DOMAŃSKI, EWA AUGUSTYNOWICZ-KOPEĆ, EWA SWOBODA-KOPEC

Polish Journal of Microbiology, Volume 68 , ISSUE 3, 303–308

Case report | 09-October-2019

Two cases of the variant RHD*DAU5 allele associated with maternal alloanti-D  

(HDFN) as D alloimmunization can occur with some D variants. Here, we describe two cases of the RHD*DAU5 allele associated with maternal alloanti-D in patients of African ancestry. Two obstetric patients were initially serologically classified as D+ with negative antibody detection tests on routine prenatal testing. Repeat testing at delivery identified anti-D in both patients with no history of RhIG administration or transfusion. DNA sequencing revealed that both patients possessed the RHD*DAU5

Jennifer A. Duncan, Susan Nahirniak, Rodrigo Onell, Gwen Clarke

Immunohematology, Volume 33 , ISSUE 2, 60–63

Report | 20-March-2020

A simple screening assay for the most common JK*0 alleles revealed compound heterozygosity in Jk(a–b–) probands from Guam

screening assay was cost-efficient when compared with DNA sequencing costs. Furthermore, selection of the more common JK*0 mutations was a practical approach that resulted in rapid identification of the genetic bases behind the Jk(a–b–) phenotypes in this unusual family. Although an obvious target for eventual inclusion into high-throughput genotyping platforms for clinical diagnostic services, current systems are very limited. Our approach provides a simple and inexpensive method for the

Elisabet Sjöberg Wester, Julia Gustafsson, Beverly Snell, Peggy Spruell, Åsa Hellberg, Martin L. Olsson, Jill R. Storry

Immunohematology, Volume 25 , ISSUE 4, 165–169

research-article | 26-April-2019

First report of Meloidogyne javanica on Ginger and Turmeric in the United States

Abolfazl Hajihassani, Weimin Ye, Brooke B. Hampton

Journal of Nematology, Volume 51 , 1–3

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