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Article | 21-July-2017

Characterization of Lilium longiflorum cv. ‘Nellie White’ Infection with Root-lesion Nematode Pratylenchus penetrans by Bright-field and Transmission Electron Microscopy

Lilium longiflorum cv. Nellie White, commonly known as Easter lily, is an important floral crop with an annual wholesale value of over $26 million in the United States. The root-lesion nematode, Pratylenchus penetrans, is a major pest of lily due to the significant root damage it causes. In this study, we investigated the cytological aspects of this plant–nematode interaction using bright-field and transmission electron microscopy. We took advantage of an in vitro culture method


Journal of Nematology, Volume 49 , ISSUE 1, 2–11

Article | 21-July-2017

Acetic Acid, 2-Undecanone, and (E)-2-Decenal Ultrastructural Malformations on Meloidogyne incognita

metabolites, that is acetic acid, (E )-2-decenal, and 2-undecanone. The commercial nematicide fosthiazate acting on acetylcholinesterase was used as control.For this reason, scanning electron microscopy and transmission electron microscopy have been employed. The acetic acid mainly harmed the cuticle, degenerated the nuclei of pseudocoel cells, and vacuolised the cytoplasm. The (E)-2-decenal and 2-undecanone did neither harm to the cuticle nor the somatic muscles but they degenerated the


Journal of Nematology, Volume 48 , ISSUE 4, 248–260

research-article | 30-November-2020

Introduction to Pristionchus pacificus anatomy

extend away from the lateral midline. The nervous system As with C. elegans and other nematodes, the majority of P. pacificus neurons are located in the head and tail. The anterior nervous system of P. pacificus, including the pharynx and anterior amphid sensory neurons, have been reconstructed from serial-section electron microscopy (Bumbarger et al., 2013; Hong et al., 2019). The nervous system of P. pacificus is remarkably similar to that of C. elegans. Both the anterior amphid sensory sensilla

Nathan E. Schroeder

Journal of Nematology, Volume 53 , 1–9

Research Article | 03-September-2018

Stauratostoma shelleyi n. gen., n. sp. (Nematoda: Rhabditida: Thelastomatidae) from Appalachian Polydesmid Millipedes (Polydesmida: Xystodesmidae)

Stauratostoma shelleyi n. gen., n. sp. is described from the midgut and hindgut of nine species of the millipede family Xystodesmidae collected in the southern Appalachian regions of North Carolina, Tennessee and Alabama. Specimens of S. shelleyi were morphologically examined with differential interference contrast, phase contrast, and scanning electron microscopy. The head of S. shelleyi differs from other thelastomatid nematodes in having a head region mushroom-shaped in profile; cruciform

Gary Phillips, Robert J. Pivar, Xiocaun Sun, John K. Moulton, Ernest C. Bernard

Journal of Nematology, Volume 50 , ISSUE 2, 133–146

Article | 21-July-2017

Coronostoma claireae n. sp. (Nematoda: Rhabditida: Oxyuridomorpha: Coronostomatidae) from the Indigenous Milliped Narceus gordanus (Chamberlain, 1943) (Diplopoda: Spirobolida) in Ocala National Forest, Florida

Twenty-four individuals of Narceus gordanus (Diplopoda: Spirobolidae) were collected in Ocala National Forest, FL, between November 2013 and July 2014. Each specimen was dissected to extract the intestine, which was removed and examined for parasitic nematodes. Coronostoma claireae n. sp. was collected from the hindgut and midgut of 10 specimens, and its morphology was examined with brightfield, differential interference contrast, phase contrast, and scanning electron microscopy. This species


Journal of Nematology, Volume 48 , ISSUE 3, 159–169

Research paper | 13-December-2017


This paper presents material and gas sensing properties of Pt/SnO2 nanowires/SiC metal oxide semiconductor devices towards hydrogen. The SnO2 nanowires were deposited onto the SiC substrates by vapor-liquid-solid growth mechanism. The material properties of the sensors were investigated using scanning electron microscopy, transmission electron microscopy and X-ray photoelectron spectroscopy. The current-voltage characteristics have been analyzed. The effective change in the barrier height for 1

M. Shafiei, K. Kalantar-zadeh, W. Wlodarski, E. Comini, M. Ferroni, G. Sberveglieri, S. Kaciulis, L. Pandolfi

International Journal on Smart Sensing and Intelligent Systems, Volume 1 , ISSUE 3, 771–783

Article | 01-September-2012


Nanocrystalline ZrO2 (Zirconia) has been synthesized by a conventional precipitation method. The structural, morphological, microstructural, optical and gas-sensing properties of ZrO2 were investigated by using X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-vis spectroscopy and static gas sensing unit, respectively. X-ray diffraction pattern and TEM of the synthesized product reveal their nano-crystalline nature with grain size

Pratap G. Patil, D. D. Kajale, V. P. Patil, G. E. Patil, G. H. Jain

International Journal on Smart Sensing and Intelligent Systems, Volume 5 , ISSUE 3, 673–684

Article | 01-March-2012


ZnO nanorods with different sizes and shapes have been successfully synthesized via a simple hydrothermal route, using zinc acetate and Cetyltriammonium bromide (CTAB) as the reactants. The thick films of as prepared ZnO were prepared by screen-printing technique in desired pattern. The films are characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The gas sensing properties of the materials have been investigated for various

Sarika D. Shinde, G. E. Patil, D. D. Kajale, D. V. Ahire, V. B. Gaikwad, G. H. Jain

International Journal on Smart Sensing and Intelligent Systems, Volume 5 , ISSUE 1, 57–70

Research Article | 20-May-2019


In this article, the secondary metabolism as a basis for antibiotics production by industrial strains of Streptomyces, Penicillium chrysogenum and Acremonium chrysogenum is discussed. Images from transmission electron microscopy reveal some important features of the mycelial cells which are related to antibiotics biosynthesis. This discovery is important for further industrial strain improvement and has economic significance. Possibilities of new strategies for antimicrobial treatment are

Wiesław Kurzątkowski, Joanna Kuczerowska

Postępy Mikrobiologii - Advancements of Microbiology, Volume 56 , ISSUE 4, 422–428

Research Article | 15-February-2020

Palladium Nanotubes for Optical Hydrogen Sensing

Arrays of freestanding upright palladium nanotubes have been fabricated using a bottom-up method of electrodepositing into anodised alumina pores. Optical transmission measurements carried out with a range of postfabrication treatment methods suggest that hydrogen diffuses into both the inner and outer surface areas of the tube structure. Furthermore, scanning electron microscopy of the same sample before and after cycling hydrogen exposure show that the structures appear undamaged by the

M. B. McAuley, A. Murphy, R. Pollard

International Journal on Smart Sensing and Intelligent Systems, Volume 7 , ISSUE 5, 1–4

research-paper | 12-August-2019

Short-term exposure to high-intensity sound induces hearing loss and apoptosis in guinea pigs

removed under a dissecting microscope and perfused with a phosphate buffer solution and fixed in 1% osmium acid for 1.5 h to prepare the samples for scanning electron microscopy (SEM) observation. The S-4800 SEM (Hitachi, Japan) was used and pictures were taken. The cochleae were isolated, washed with phosphate buffer and fixed with 1% osmium acid for 1 h. Then, the samples were dehydrated by gradient alcohol and embedded in epoxy resin to prepare the area for transmission electron microscopy (TEM

Yanqing Liu, Hui Wang, Ziquan Liu, Yanjun Gu, Limin Xin, Jinyang Liu, Haojun Fan

Acta Neurobiologiae Experimentalis, Volume 79 , ISSUE 2, 155–168

Article | 01-March-2012

Humidity sensor based on silver nanoparticles embedded in a polymeric coating

In this work, it is presented a novel optical fiber humidity sensor based on silver nanoparticle-loaded polymeric coatings built onto an optical fiber core. The polymeric film was fabricated using the Layer-by-Layer assembly technique. The silver nanoparticles (Ag NPs) were characterized using transmission electron microscopy (TEM and UV-VIS spectroscopy. A Localized Surface Plasmon Resonance (LSPR) attenuation band is observed when the thickness of the coating increases, and showed a very good

Pedro J. Rivero, Aitor Urrutia, Javier Goicoechea, Francisco J. Arregui, Ignacio R. Matías

International Journal on Smart Sensing and Intelligent Systems, Volume 5 , ISSUE 1, 71–83

Article | 01-September-2012


MoO3 thin films have been prepared by a simple spray pyrolysis technique at substrate temperature 250oC. The structure and morphology of thin films are characterized by X-ray powder diffraction (XRD), scanning electron microscopy and UV-vis spectroscopy. The gas sensing properties of MoO3 thin film is studied at gas concentration 400 ppm and working temperature of 100–400 oC. It was found that the sensitivity depended on the working temperatures and also H2S gas concentration. The results show

D. V. Ahire, S. D. Shinde, G. E. Patil, K. K. Thakur, V. B. Gaikwad, V. G. Wagh, G. H. Jain

International Journal on Smart Sensing and Intelligent Systems, Volume 5 , ISSUE 3, 592–605

Research paper | 01-June-2011


In this paper, composition analysis using scanning electron microscopy/energy-dispersive X-ray spectroscopy analysis (SEM-EDX) and laser-induced breakdown spectroscopy (LIBS) for particulate matter in diesel exhaust is presented. Conventionally, scanning mobility particle sizer (SMPS) has been widely used to obtain particle size distribution of the suspended particles. However, real-time analysis of the composition of diesel particulate matter is difficult because of the small sized particles

S. Ikezawa, M. Wakamatsu, T. Ueda

International Journal on Smart Sensing and Intelligent Systems, Volume 4 , ISSUE 2, 174–188

Research Article | 03-December-2018

Description of Gracilacus paralatescens n. sp. (Nematoda:Paratylenchinae) found from the rhizosphere of Bamboo in Zhejiang, China

Munawar Maria, Ruihang Cai, Weimin Ye, Thomas O. Powers, Jingwu Zheng

Journal of Nematology, Volume 50 , ISSUE 4, 611–622

Article | 21-July-2017

Redefinition of Genus Malenchus Andrassy, 1968 (Tylenchomorpha: Tylenchidae) with Additional Data on Ecology

Malenchus is the second specious genus in Tylenchidae. In the presented study, we examined 22 populations including 12 type/paratype species. Detailed morphology was recovered using light microscopy, scanning- and transmission- electron microscopy. All population and type slides were recorded as picture and video vouchers, which are available online. We have compared inter- or intraspecific variations and extracted taxonomically informative traits. Amended definitions of the Malenchus as well


Journal of Nematology, Volume 49 , ISSUE 2, 189–206

Article | 28-August-2018


improve fresh concrete properties, density and corrosion resistance of concrete. The chemical reasons of such effect were explained by the means of x-ray diffraction, differential thermogravimetry and scanning electron microscopy. This development should be widely used in several projects in Ukraine, where sulphate-resistant concrete is needed.


Architecture, Civil Engineering, Environment, Volume 10 , ISSUE 4, 101–106

Research Article | 15-February-2020

Optical Detection of Herbicides in Water using Dye- Modified Single Walled Carbon Nanotubes

Hybrid materials were produced by mixing CuPcR4 with acidified single-walled carbon nanotubes (SWCNTs) and characterised by UV-Vis absorption spectroscopy, scanning electron microscopy and atomic force microscopy. Thin films of pristine CuPcR4 and SWCNT/CuPcR4 were prepared by spin coating onto gold-coated glass slides and applied as active layers to detect pentachlorophenol (PCP), 2-chlorophenol, diuron and simazine in water utilizing Total Internal Reflection Ellipsometry (TIRE) as an optical

Hikmat Banimuslem, Aseel Hassan, Tamara Basova, Asuman Dakoğlu Gülmez, Mahmut Durmuş, Ayşe Gül Gürek, Vefa Ahsen

International Journal on Smart Sensing and Intelligent Systems, Volume 7 , ISSUE 5, 1–5

research-article | 30-November-2019

First report of Ovomermis sinensis (Nematoda: Mermithidae) parasitizing fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) in China

Bingjiao Sun, Fen Li, Xiaorui He, Fengqin Cao, Elizabeth Bandason, David Shapiro-Ilan, Weibin Ruan, Shaoying Wu

Journal of Nematology, Volume 52 , 1–7

research-article | 30-November-2018

Updated description of Paratylenchus lepidus Raski 1975 and P. minor Sharma, Sharma and Khan, 1986 by integrating molecular and ultra-structural observations

: Scanning electron microscopy of Paratylenchus lepidus (Raski, 1975a, 1975b). (A-C) Lip regions; (D, E) vulval regions; (F, G) posterior region arrows showing position of vulva; (H-K) female tails (Scale bars, A-C = 5 μm; D-K = 10 μm). Measurements Measurements of the females of P. lepidus Taizhou population are given in Table 1. Table 1. Morphometric data for Paratylenchus lepidus (Raski, 1975a, 1975b). Type Additional type pop. This study Raski (1975b) Raski (1975b

Munawar Maria, Wentao Miao, Weimin Ye, Jingwu Zheng

journal of nematology, Volume 51 , 1–13

research-article | 30-November-2018

Meloidogyne aegracyperi n. sp. (Nematoda: Meloidogynidae), a root-knot nematode parasitizing yellow and purple nutsedge in New Mexico

made from specimens mounted on 5% water agar pads and paralyzed with 0.1 m sodium azide (Eisenback, 2012). Females and J2 were prepared for scanning electron microscopy (SEM) according to Eisenback (1985). Perineal patterns were prepared for SEM according to Charchar and Eisenback (2001). They were observed and photographed by LM with negative contrast as reported by Eisenback (2012). Eggs were measured in fresh tap water mounted on agar pads. All LM observations except for perineal patterns were

J. D. Eisenback, L. A. Holland, J. Schroeder, S. H. Thomas, J. M. Beacham, S. F. Hanson, V. S. Paes-Takahashi, P. Vieira

journal of nematology, Volume 51 , 1–16

research-article | 18-March-2020

A new rare nematode Nothocriconemoides hangzhouensis n. sp. (Nematoda: Criconematidae) from Hangzhou, China

spherical, filled with sperm. Vagina is straight, vulva is closed, and vulval lips do not project above body contour. The ventral side of postvulval annuli is inverted, in majority of individuals. Anus is indistinct and located on the next annuli posterior to vulva. Tail is short and conoid, longitudinal striations are more prominent on the terminal annulus that gives the appearance of forked or branched terminus. Figure 4: Scanning electron microscopy of Nothocriconemoides hangzhouensis n. sp. Female

Munawar Maria, Wentao Miao, Ruihang Cai, Pablo Castillo, Jingwu Zheng

Journal of Nematology, Volume 52 , 1–14

research-article | 16-April-2020

A new stunt nematode, Geocenamus chengi n. sp. (Nematoda: Merliniinae) in the rhizosphere of tea (Camellia sinensis) from Zhejiang Province, China

. sp. Male. A: entire body; pharyngeal regions, arrow pointing on the excretory pore (exp); C: tail with bursa; D: gonad; E, F: tail in ventral view; G, I: male tail arrows pointing on phasmid (ph) (Scale bars = A = 100 μm; B-I = 10 μm). Figure 4: Scanning electron microscopy of Geocenamus chengi n. sp. A: anterior region; B-C: en face view; D-F: vulval regions; G, H: female tail regions arrows pointing anus (a); I: anal area at higher resolution, arrow pointing on the position of anus; J

Munawar Maria, Wentao Miao, Pablo Castillo, Jingwu Zheng

Journal of Nematology, Volume 52 , 1–13

Research Article | 15-February-2020

Method to Convert a Horizontal Furnace to Grow ZnO Nanowires for Gas Sensing by the VLS Method

. Under a constant flow of Argon gas (200mL/min), the substrates along with a mixture of ZnO and graphite powder are placed in a modified horizontal furnace which uses a 2.54 cm internally mounted quartz tube and are exposed to high temperature (527°C-1000°C) for different growth times. This reaction causes the formation of ZnO nanowires on the substrates. scanning electron microscopy is used to measure the size of the nanowires.

Sushma Nandhyala, Michael Haji-Sheikh, Martin Kocanda, Suma Rajashankar

International Journal on Smart Sensing and Intelligent Systems, Volume 7 , ISSUE 5, 1–5

Research Article | 17-October-2018

Characterization of Meloidogyne indica (Nematoda: Meloidogynidae) Parasitizing Neem in India, with a Molecular Phylogeny of the Species

juveniles, males and females were carried out by light compound and scanning electron microscopy. Gross morphology and measurements were found consistent with the original description of M. indica infecting citrus by Whitehead (1968). The neem population was found to infect and reproduce on citrus. Additionally, evolutionary relationship was deduced by Maximum likelihood method using ITS rRNA, D2D3 expansion segment of 28S rRNA and mitochondrial COI sequences. Phylogenetic analyses based on these

Victor Phani, Satyapal Bishnoi, Amita Sharma, Keith G. Davies, Uma Rao

Journal of Nematology, Volume 50 , ISSUE 3, 387–398

Research Article | 03-December-2018

Nematotoxic coumarins from Angelica pubescens Maxim. f. biserrata Shan et Yuan roots and their physiological effects on Bursaphelenchus xylophilus

magnetic resonance spectral data analysis. The LC50 values against B. xylophilus in 72 hr were 489.17, 406.74, 430.08, and 435.66 μM, respectively. These compounds also altered the smooth morphology of the B. xylophilus exoskeleton to a rough and pitted appearance as visualized by electron microscopy. The coumarins 1-4 possessed significant acetylcholinesterase inhibitory activities but had negligible effects on amylase and cellulase. This research provides additional clues to the nematotoxic mechanism

Qun-Qun Guo, Gui-Cai Du, Yong-Xing Li, Chen-Yan Liang, Chao Wang, Ya-Nan Zhang, Rong-Gui Li

Journal of Nematology, Volume 50 , ISSUE 4, 559–568

Article | 01-March-2012


ZnO nano-particles have been synthesized by simple chemical route using a starting solution consisting of zinc acetate and citric acid as a surfactant agent. The structural properties of the prepared ZnO nano-particles annealed at different temperatures have been characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM) analyses. The XRD patterns show ZnO-wurtize phase in the nano-powders, and size of crystals increases by increasing the annealing temperatures. The TEM

Sarika D. Shinde, G. E. Patil, D. D. Kajale, V. G. Wagh, V. B. Gaikwad, G. H. Jain

International Journal on Smart Sensing and Intelligent Systems, Volume 5 , ISSUE 1, 277–294

research-article | 30-November-2019

Ultrastructure of Hirschmanniella diversa early-stage infection in browning rhizomes of Indian lotus

known about these properties in H. diversa (Liu and Yu, 2002; Uematsu et al., 2015, 2016). Liu and Yu (2002) observed the morphology of H. diversa using scanning electron microscopy, and Uematsu et al. (2015) described three weed species parasitized by H. diversa in Indian lotus fields. Light and electron microscopy analysis of Indian lotus rootlets revealed all stages of the H. diversa lifecycle, from the egg to the juvenile and adult forms (Uematsu et al., 2016). However, the parasitic behavior of

Shigeru Uematsu, Tetsuo Yabu, Mitsuyoshi Yao, Takayuki Kurihara, Hironori Koga

Journal of Nematology, Volume 52 , 1–9

research-article | 30-November-2019

Additional notes on the morphology and molecular data of the Kikuyu root-knot nematode, Meloidogyne kikuyensis (Nematoda: Meloidogynidae)

nodule-like gall were elucidated by light, scanning, and transmission electron microscopy (Eisenback and Dodge, 2012; Dodge, 2014). The galls induced by M. kikuyensis are unique and more complex than those caused by most root-knot nematode species. The vascular tissues that supply the giant cells with nutrients occur at a right angle to the vascular cylinder in the main root. Unlike most species of root-knot nematodes, feeding cells of M. kikuyensis appear to be formed by the dissolution of cell

J. D. Eisenback, P. Vieira

Journal of Nematology, Volume 52 , 1–13

research-article | 30-November-2020

First detection of Gongylonema species in Geotrupes mutator in Europe

: Scanning electron microscopy (SEM) of Gongylonema sp. (1) Anterior portion of a specimen L3 without cuticular bosses and highlighting the lateral ala (La). (2) Central view of the cephalic end, highlighting the amphids (Am), 4 cervical papillae (Cp), 4 internal papillae (Ip), 6 inner lateral papilla (ILp), peribuccal collar (Pc) and cuticular plates on the margins of the buccal opening. (3) Detail of lateral alae (La) and excretory pore (Ep). (4) Details of excretory pore (Ep). (5) Posterior portion of

Daniel Bravo-Barriga, Manuel Martín-Pérez, Jorge M. Lobo, Ricardo Parreira, Juan Enrique Pérez-Martín, Eva Frontera

Journal of Nematology, Volume 53 , 1–9

research-article | 24-April-2020

Cephalenchus driekieae n. sp. (Nematoda: Tylenchidae) from South Africa, a new member of the genus with a long pharyngeal overlap

as template to redraw in CorelDRAW® software version 2018. Scanning electron microscopy (SEM) For SEM, the nematodes mounted on permanent slides were cleaned and hydrated in distilled water, dehydrated in a graded ethanol-acetone series, critical point dried, coated with gold, and observed with a Zeiss Merlin microscope (5 kV) (Zeiss, Oberkochen, Germany) (Abolafia, 2015). DNA extraction, PCR reaction, and gel electrophoresis DNA extraction and PCR conditions followed Rashidifard et al. (2019

Milad Rashidifard, Gerhard Du Preez, Joaquín Abolafia, Majid Pedram

Journal of Nematology, Volume 52 , 1–10

research-article | 22-February-2021

Rotylenchus wimbii n. sp. (Nematoda: Hoplolaimidae) associated with finger millet in Kenya

Kenya. The potential importance of this nematode as a pest of finger millet was evidenced by its dense population in the soil sample and its presence observed in the finger millet root system (revealed by the fuchsin staining method). Nematode characterization was carried out based on morphological information obtained from light microscopy (LM) and scanning electron microscopy (SEM) studies. Illustrations, morphometrics, and molecular information of ITS, 18S, and 28S of rDNA and COI of mtDNA are

Phougeishangbam Rolish Singh, Gerrit Karssen, Kelvin Gitau, Cecilia Wanjau, Marjolein Couvreur, Njira Njira Pili, Godelieve Gheysen, Wim Bert

Journal of Nematology, Volume 53 , 1–14

research-article | 11-March-2021

Morphological and molecular characters of Scutellonema brachyurus (Steiner, 1938) Andrássy, 1958 from South Africa

drawing tube (camera lucida) attached to an Omax microscope (China). Pictures were taken with a Nikon Eclipse 80i light microscope provided with differential interference contrast optics (DIC) and a Nikon Digital Sight DS-U1 camera (Nikon, Tokyo, Japan). Micrographs were edited using Adobe® Photoshop® CS. The terminology used for the morphology of stoma follows the proposals by Baldwin et al. (2004). Scanning electron microscopy (SEM) Specimens preserved in glycerine were selected for observation

Ebrahim Shokoohi

journal of nematology, Volume 53 , 1–13

Research Article | 03-September-2018

First Report of Carrot Cyst Nematode Heterodera carotae in Mexico: Morphological, Molecular Characterization, and Host Range Study

was made using light and scanning electron microscopy of the second stage juveniles, females, males and cysts, and the host range study, was performed with nine different plants from five families. The molecular identification was made by sequencing and analysing the ITS rRNA and partial COI genes. It was shown that using presently available molecular tools it is not possible to make an accurate differentiation of H. carotae from H. cruciferae. The host range test allowed to distinguish these

Ilia Mariana Escobar-Avila, Edgar Óliver López-Villegas, Sergei A. Subbotin, Alejandro Tovar-Soto

Journal of Nematology, Volume 50 , ISSUE 2, 229–242

Research paper | 10-October-2018

Effects of PINK1 mutation on synapses and behavior in the brain of Drosophila melanogaster

Bruchpilot (BRP), a presynaptic scaffolding protein that is crucial for neurotransmission in all type of synapses in Drosophila. In addition, other proteins that are involved in synaptic transmission; Rab5, Syntaxin and Wishful Thinking were also decreased in abundance in mutants, except Synaptotagmin. Transmission electron microscopy (TEM) also confirmed less and abnormal synaptic vesicles at tetrad synapses in the visual system of PINK1 mutants. The lower level of BRP and longer day sleep observed was

Bartosz Doktór, Milena Damulewicz, Wojciech Krzeptowski, Barbara Bednarczyk, Elżbieta Pyza

Acta Neurobiologiae Experimentalis, Volume 78 , ISSUE 3, 231–241

research-article | 30-November-2018

New data on known species of Hirschmanniella and Pratylenchus (Rhabditida, Pratylenchidae) from Iran and South Africa

. Scanning electron microscopy (SEM) Specimens preserved in glycerine were selected for observation under SEM according to Abolafia (2015). The nematodes were hydrated in distilled water, dehydrated in a graded ethanol-acetone series, critical point dried, coated with gold, and observed with a Zeiss Merlin microscope (5 kV) (Zeiss, Oberkochen, Germany). Statistical analysis Principal component analysis and the correlation of morphometric data using the Pearson method was done by XLSTAT (Addinsoft, 2007

Ebrahim Shokoohi, Joaquín Abolafia, Phatu William Mashela, Nafiseh Divsalar

Journal of Nematology, Volume 51 , 1–26

research-article | 30-November-2019

Hemicycliophora ahvasiensis n. sp. (Nematoda: Hemicycliophoridae), and data on a known species, from Iran

. Scanning electron microscopy (SEM) Specimens preserved in glycerin were selected for observation according to Abolafia (2015). They were hydrated in distilled water, dehydrated in a graded mixture of ethanol-acetone series, critical point-dried with liquid carbon dioxide, and coated with gold. The mounts were examined with a Zeiss Merlin microscope (5 kV). DNA extraction, PCR and sequencing For molecular analyses, single female specimens were picked out, examined in a drop of distilled water on a

Sedighe Azimi, Joaquín Abolafia, Majid Pedram

Journal of Nematology, Volume 52 , 1–19

Research Article | 31-May-2018

Incidence of Oscheius onirici (Nematoda: Rhabditidae), a potentially entomopathogenic nematode from the marshlands of Wisconsin, USA

In a search for an entomopathogenic nematode to control cranberry insect pests, three Oscheius populations (Rhabditidae) were recovered through the Galleria-bait method from one sample taken in a wild cranberry marsh in Jackson County, Wisconsin, USA. Morphological studies with light microscopy and scanning electron microscopy, as well as molecular analyses of the near-full-length small subunit rDNA gene, D2/D3 expansion segments of the large subunit rDNA gene, internal transcribed spacer, and

Weimin Ye, Shane Foye, Ann E. MacGuidwin, Shawn Steffan

Journal of Nematology, Volume 50 , ISSUE 1, 9–26

research-article | 30-November-2020

Pratylenchus smoliki, a new nematode species (Pratylenchidae: Tylenchomorpha) from the Great Plains region of North America

. Both adult females and males, as well as juveniles, were subjected to morphological and molecular analyses. Voucher specimens were fixed in 4% formaldehyde plus 2% glycerol, and the slow evaporation method was used prior to mounting. Nematodes were prepared for scanning electron microscopy (SEM) by fixation in 4% glutaraldehyde followed by post-fixation with 2% Osmium Tetroxide, dehydration in a graded series of alcohol to 100% ethyl alcohol, critical point drying, mounting on SEM specimen stubs

Thomas Powers, Timothy Todd, Tim Harris, Rebecca Higgins, Ann MacGuidwin, Peter Mullin, Mehmet Ozbayrak, Kirsten Powers, Kanan Sakai

Journal of Nematology, Volume 53 , 1–23

research-article | 30-November-2018

Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western Australia

Pingelly with typical P. curvicauda features were fixed in 4% formaldehyde solution and sent to an expert taxonomist in the UK for further characterization. The detailed morphometric measurements obtained were compared with those taken in Australia. Preparation of nematode samples for scanning electron microscopy Scanning electron microscopy (SEM) was used to further characterize P. curvicauda. To do this, single nematodes were fixed in 3% glutaraldehyde in 0.025 M phosphate buffer (pH 7.0) overnight

Farhana Begum, John Fosu-Nyarko, Shashi Sharma, Bill Macleod, Sarah Collins, Michael G. K. Jones

Journal of Nematology, Volume 51 , 1–15

research-article | 30-November-2020

Morphological and molecular characterization of Paratylenchus beltsvillensis n. sp. (Tylenchida: Paratylenchidae) from the rhizosphere of pine tree (Pinus virginiana Mill) in Maryland, USA

Paratylechus beltsvillensis n. sp. The objective of this study was also to describe this new species using light (LM) and scanning electron microscopy (SEM) and provide its molecular characterization. Materials and methods Nematode samples In September and October of 2020, few soil samples were collected in the Little Paint Branch Park, Beltsville, Prince George’s County, Maryland, USA And sent to the Plant Pest Diagnostic Center, California Department of Food and Agriculture, Sacramento, CA and part of

Mihail R. Kantor, Zafar A. Handoo, Sergei A. Subbotin, Gary R. Bauchan, Joseph D. Mowery

Journal of Nematology, Volume 53 , 1–10

Research paper | 25-July-2017

Surgical injury-induced early neocortical microvascular changes and characteristics of the cells populating the peri-lesion zone

remodeling of brain parenchyma, including the presence of multiple cell types of immature phenotypes. The latter, as shown by a variety of light and electron microscopy techniques, included endothelial cell precursors as well as nestin-positive immature neural cells of astrocytic or non-glial characteristics. However, there was no evidence of in situ neurogenesis or a considerable migration of cells from SVZ. The centers of the said repair processes were capillary blood vessels connected with basal

Dorota Sulejczak, Stanisław J. Chrapusta, Wojciech Kozłowski, Małgorzata Frontczak-Baniewicz

Acta Neurobiologiae Experimentalis, Volume 76 , ISSUE 2, 125–141

Original Paper | 28-June-2017

Intracellular Siderophore Detection in an Egyptian, Cobalt-Treated F. solani Isolate Using SEM-EDX with Reference to its Tolerance

An Egyptian, plant pathogenic Fusarium solani isolate was grown on cobalt concentrations of 0, 50, 200, 500, 800, and 1000 ppm. The iso­late survived concentrations up to 800 ppm, however failed to grow at 1000 ppm. Morphology and elemental analysis of the isolate under the investigated Co concentrations were studied using Scanning electron microscopy (SEM) and energy dispersive X-ray microanalysis (EDX). The isolate reserved its morphology up to a concentration of 200 ppm. Morphological

Farrag M. Rasha

Polish Journal of Microbiology, Volume 66 , ISSUE 2, 235–243

Research Article | 26-September-2018

Pathogenic Potential of Fresh, Frozen, and Thermally Treated Anisakis spp. Type II (L3) (Nematoda: Anisakidae) after Oral Inoculation into Wistar Rats: A Histopathological Study

dissected to investigate the presence of nematode larvae. Thirty fish (42.9%) of the total were parasitized by nematode larvae which were morphologically identified as Anisakis spp. Type II (L3) according to light and scanning electron microscopy. The pathogenic potential of oral inoculation of fresh, frozen, and thermally treated larvae into Wistar rats was elucidated by histological examination of their thymus and spleen. Results obtained indicated that neither cooling nor freezing of the parasite


Journal of Nematology, Volume 49 , ISSUE 4, 427–436

Research paper | 06-February-2018

Effects of epidural compression on stellate neurons and thalamocortical afferent fibers in the rat primary somatosensory cortex

-lesion. Anterograde tracing analyses exhibited a progressive decline of TCA fiber density in barrel field for 6 months post-lesion. Due to the abrupt decrease of TCA fiber density at 3 days after compression, we further used electron microscopy to investigate the ultrastructure of TCA fibers at this time. Some TCA fiber terminal profiles with dissolved or darkened mitochondria and fewer synaptic vesicles were distorted and broken. Furthermore, the disruption of mitochondria and myelin sheath was

Tzu-Yin Yeh, Guo-Fang Tseng, Chi-Yu Tseng, Yung-Hsin Huang, Pei-Hsin Liu

Acta Neurobiologiae Experimentalis, Volume 77 , ISSUE 1, 1–17

research-article | 30-November-2020

Laimaphelenchus africanus n. sp. (Tylenchomorpha: Aphelenchoididae) from South Africa, a morphological and molecular phylogenetic study, with an update to the diagnostics of the genus

E600 light microscope. Photomicrographs were taken using an Olympus DP72 digital camera attached to an Olympus BX51 microscope equipped with differential interference contrast. Drawings were made using a drawing tube attached to the microscope and were redrawn using the CorelDRAW® software version 2017. Scanning electron microscopy For the scanning electron microscopy, specimens preserved in glycerine were selected for observation under SEM according to the Abolafia’s (2015) protocol. The

Farahnaz Jahanshahi Afshar, Milad Rashidifard, Joaquín Abolafia, Miloslav Zouhar, Hendrika Fourie, Majid Pedram

Journal Of Nematology, Volume 53 , 1–14

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