Article | 21-July-2017
Lilium longiflorum cv. Nellie White, commonly known as Easter lily, is an important floral crop with an annual wholesale value of over $26 million in the United States. The root-lesion nematode, Pratylenchus penetrans, is a major pest of lily due to the significant root damage it causes. In this study, we investigated the cytological aspects of this plant–nematode interaction using bright-field and transmission electron microscopy. We took advantage of an in vitro culture method
PAULO VIEIRA,
JOSEPH MOWERY,
JAMES KILCREASE,
JONATHAN D. EISENBACK,
KATHRYN KAMO
Journal of Nematology, Volume 49 , ISSUE 1, 2–11
Article | 21-July-2017
metabolites, that is acetic acid, (E )-2-decenal, and 2-undecanone. The commercial nematicide fosthiazate acting on acetylcholinesterase was used as control.For this reason, scanning electron microscopy and transmission electron microscopy have been employed. The acetic acid mainly harmed the cuticle, degenerated the nuclei of pseudocoel cells, and vacuolised the cytoplasm. The (E)-2-decenal and 2-undecanone did neither harm to the cuticle nor the somatic muscles but they degenerated the
NIKOLETTA NTALLI,
MARLENA RATAJCZAK,
CHRISOSTOMOS OPLOS,
URANIA MENKISSOGLU-SPIROUDI,
ZBIGNIEW ADAMSKI
Journal of Nematology, Volume 48 , ISSUE 4, 248–260
research-article | 30-November-2020
extend away from the lateral midline.
The nervous system
As with C. elegans and other nematodes, the majority of P. pacificus neurons are located in the head and tail. The anterior nervous system of P. pacificus, including the pharynx and anterior amphid sensory neurons, have been reconstructed from serial-section electron microscopy (Bumbarger et al., 2013; Hong et al., 2019). The nervous system of P. pacificus is remarkably similar to that of C. elegans. Both the anterior amphid sensory sensilla
Nathan E. Schroeder
Journal of Nematology, Volume 53 , 1–9
Research Article | 03-September-2018
Stauratostoma shelleyi n. gen., n. sp. is described from the midgut and hindgut of nine species of the millipede family Xystodesmidae collected in the southern Appalachian regions of North Carolina, Tennessee and Alabama. Specimens of S. shelleyi were morphologically examined with differential interference contrast, phase contrast, and scanning electron microscopy. The head of S. shelleyi differs from other thelastomatid nematodes in having a head region mushroom-shaped in profile; cruciform
Gary Phillips,
Robert J. Pivar,
Xiocaun Sun,
John K. Moulton,
Ernest C. Bernard
Journal of Nematology, Volume 50 , ISSUE 2, 133–146
Article | 21-July-2017
Twenty-four individuals of Narceus gordanus (Diplopoda: Spirobolidae) were collected in Ocala National Forest, FL, between November 2013 and July 2014. Each specimen was dissected to extract the intestine, which was removed and examined for parasitic nematodes. Coronostoma claireae n. sp. was collected from the hindgut and midgut of 10 specimens, and its morphology was examined with brightfield, differential interference contrast, phase contrast, and scanning electron microscopy. This species
GARY PHILLIPS,
ERNEST C. BERNARD,
ROBERT J. PIVAR,
JOHN K. MOULTON,
ROWLAND M. SHELLEY
Journal of Nematology, Volume 48 , ISSUE 3, 159–169
Research paper | 13-December-2017
This paper presents material and gas sensing properties of Pt/SnO2 nanowires/SiC metal oxide semiconductor devices towards hydrogen. The SnO2 nanowires were deposited onto the SiC substrates by vapor-liquid-solid growth mechanism. The material properties of the sensors were investigated using scanning electron microscopy, transmission electron microscopy and X-ray photoelectron spectroscopy. The current-voltage characteristics have been analyzed. The effective change in the barrier height for 1
M. Shafiei,
K. Kalantar-zadeh,
W. Wlodarski,
E. Comini,
M. Ferroni,
G. Sberveglieri,
S. Kaciulis,
L. Pandolfi
International Journal on Smart Sensing and Intelligent Systems, Volume 1 , ISSUE 3, 771–783
Article | 01-September-2012
Nanocrystalline ZrO2 (Zirconia) has been synthesized by a conventional precipitation method. The structural, morphological, microstructural, optical and gas-sensing properties of ZrO2 were investigated by using X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-vis spectroscopy and static gas sensing unit, respectively. X-ray diffraction pattern and TEM of the synthesized product reveal their nano-crystalline nature with grain size
Pratap G. Patil,
D. D. Kajale,
V. P. Patil,
G. E. Patil,
G. H. Jain
International Journal on Smart Sensing and Intelligent Systems, Volume 5 , ISSUE 3, 673–684
Article | 01-March-2012
ZnO nanorods with different sizes and shapes have been successfully synthesized via a simple hydrothermal route, using zinc acetate and Cetyltriammonium bromide (CTAB) as the reactants. The thick films of as prepared ZnO were prepared by screen-printing technique in desired pattern. The films are characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The gas sensing properties of the materials have been investigated for various
Sarika D. Shinde,
G. E. Patil,
D. D. Kajale,
D. V. Ahire,
V. B. Gaikwad,
G. H. Jain
International Journal on Smart Sensing and Intelligent Systems, Volume 5 , ISSUE 1, 57–70
Research Article | 20-May-2019
In this article, the secondary metabolism as a basis for antibiotics production by industrial strains of Streptomyces, Penicillium chrysogenum and Acremonium chrysogenum is discussed. Images from transmission electron microscopy reveal some important features of the mycelial cells which are related to antibiotics biosynthesis. This discovery is important for further industrial strain improvement and has economic significance. Possibilities of new strategies for antimicrobial treatment are
Wiesław Kurzątkowski,
Joanna Kuczerowska
Postępy Mikrobiologii - Advancements of Microbiology, Volume 56 , ISSUE 4, 422–428
Research Article | 15-February-2020
Arrays of freestanding upright palladium nanotubes have been fabricated using a bottom-up method of electrodepositing into anodised alumina pores. Optical transmission measurements carried out with a range of postfabrication treatment methods suggest that hydrogen diffuses into both the inner and outer surface areas of the tube structure. Furthermore, scanning electron microscopy of the same sample before and after cycling hydrogen exposure show that the structures appear undamaged by the
M. B. McAuley,
A. Murphy,
R. Pollard
International Journal on Smart Sensing and Intelligent Systems, Volume 7 , ISSUE 5, 1–4
research-paper | 12-August-2019
removed under a dissecting microscope and perfused with a phosphate buffer solution and fixed in 1% osmium acid for 1.5 h to prepare the samples for scanning electron microscopy (SEM) observation. The S-4800 SEM (Hitachi, Japan) was used and pictures were taken.
The cochleae were isolated, washed with phosphate buffer and fixed with 1% osmium acid for 1 h. Then, the samples were dehydrated by gradient alcohol and embedded in epoxy resin to prepare the area for transmission electron microscopy (TEM
Yanqing Liu,
Hui Wang,
Ziquan Liu,
Yanjun Gu,
Limin Xin,
Jinyang Liu,
Haojun Fan
Acta Neurobiologiae Experimentalis, Volume 79 , ISSUE 2, 155–168
Article | 01-March-2012
In this work, it is presented a novel optical fiber humidity sensor based on silver nanoparticle-loaded polymeric coatings built onto an optical fiber core. The polymeric film was fabricated using the Layer-by-Layer assembly technique. The silver nanoparticles (Ag NPs) were characterized using transmission electron microscopy (TEM and UV-VIS spectroscopy. A Localized Surface Plasmon Resonance (LSPR) attenuation band is observed when the thickness of the coating increases, and showed a very good
Pedro J. Rivero,
Aitor Urrutia,
Javier Goicoechea,
Francisco J. Arregui,
Ignacio R. Matías
International Journal on Smart Sensing and Intelligent Systems, Volume 5 , ISSUE 1, 71–83
Article | 01-September-2012
MoO3 thin films have been prepared by a simple spray pyrolysis technique at substrate temperature 250oC. The structure and morphology of thin films are characterized by X-ray powder diffraction (XRD), scanning electron microscopy and UV-vis spectroscopy. The gas sensing properties of MoO3 thin film is studied at gas concentration 400 ppm and working temperature of 100–400 oC. It was found that the sensitivity depended on the working temperatures and also H2S gas concentration. The results show
D. V. Ahire,
S. D. Shinde,
G. E. Patil,
K. K. Thakur,
V. B. Gaikwad,
V. G. Wagh,
G. H. Jain
International Journal on Smart Sensing and Intelligent Systems, Volume 5 , ISSUE 3, 592–605
Research paper | 01-June-2011
In this paper, composition analysis using scanning electron microscopy/energy-dispersive X-ray spectroscopy analysis (SEM-EDX) and laser-induced breakdown spectroscopy (LIBS) for particulate matter in diesel exhaust is presented. Conventionally, scanning mobility particle sizer (SMPS) has been widely used to obtain particle size distribution of the suspended particles. However, real-time analysis of the composition of diesel particulate matter is difficult because of the small sized particles
S. Ikezawa,
M. Wakamatsu,
T. Ueda
International Journal on Smart Sensing and Intelligent Systems, Volume 4 , ISSUE 2, 174–188
Research Article | 03-December-2018
Munawar Maria,
Ruihang Cai,
Weimin Ye,
Thomas O. Powers,
Jingwu Zheng
Journal of Nematology, Volume 50 , ISSUE 4, 611–622
Article | 21-July-2017
Malenchus is the second specious genus in Tylenchidae. In the presented study, we examined 22 populations including 12 type/paratype species. Detailed morphology was recovered using light microscopy, scanning- and transmission- electron microscopy. All population and type slides were recorded as picture and video vouchers, which are available online. We have compared inter- or intraspecific variations and extracted taxonomically informative traits. Amended definitions of the Malenchus as well
XUE QING,
WIM BERT
Journal of Nematology, Volume 49 , ISSUE 2, 189–206
Article | 28-August-2018
improve fresh concrete properties, density and corrosion resistance of concrete. The chemical reasons of such effect were explained by the means of x-ray diffraction, differential thermogravimetry and scanning electron microscopy. This development should be widely used in several projects in Ukraine, where sulphate-resistant concrete is needed.
Artem PRYYMACHENKO,
Leonid SHEINICH
Architecture, Civil Engineering, Environment, Volume 10 , ISSUE 4, 101–106
Research Article | 15-February-2020
Hybrid materials were produced by mixing CuPcR4 with acidified single-walled carbon nanotubes (SWCNTs) and characterised by UV-Vis absorption spectroscopy, scanning electron microscopy and atomic force microscopy. Thin films of pristine CuPcR4 and SWCNT/CuPcR4 were prepared by spin coating onto gold-coated glass slides and applied as active layers to detect pentachlorophenol (PCP), 2-chlorophenol, diuron and simazine in water utilizing Total Internal Reflection Ellipsometry (TIRE) as an optical
Hikmat Banimuslem,
Aseel Hassan,
Tamara Basova,
Asuman Dakoğlu Gülmez,
Mahmut Durmuş,
Ayşe Gül Gürek,
Vefa Ahsen
International Journal on Smart Sensing and Intelligent Systems, Volume 7 , ISSUE 5, 1–5
research-article | 30-November-2019
Bingjiao Sun,
Fen Li,
Xiaorui He,
Fengqin Cao,
Elizabeth Bandason,
David Shapiro-Ilan,
Weibin Ruan,
Shaoying Wu
Journal of Nematology, Volume 52 , 1–7
research-article | 30-November-2018
:
Scanning electron microscopy of Paratylenchus lepidus (Raski, 1975a, 1975b). (A-C) Lip regions; (D, E) vulval regions; (F, G) posterior region arrows showing position of vulva; (H-K) female tails (Scale bars, A-C = 5 μm; D-K = 10 μm).
Measurements
Measurements of the females of P. lepidus Taizhou population are given in Table 1.
Table 1.
Morphometric data for Paratylenchus lepidus (Raski, 1975a, 1975b).
Type
Additional type pop.
This study
Raski (1975b)
Raski (1975b
Munawar Maria,
Wentao Miao,
Weimin Ye,
Jingwu Zheng
journal of nematology, Volume 51 , 1–13
research-article | 30-November-2018
made from specimens mounted on 5% water agar pads and paralyzed with 0.1 m sodium azide (Eisenback, 2012). Females and J2 were prepared for scanning electron microscopy (SEM) according to Eisenback (1985). Perineal patterns were prepared for SEM according to Charchar and Eisenback (2001). They were observed and photographed by LM with negative contrast as reported by Eisenback (2012). Eggs were measured in fresh tap water mounted on agar pads. All LM observations except for perineal patterns were
J. D. Eisenback,
L. A. Holland,
J. Schroeder,
S. H. Thomas,
J. M. Beacham,
S. F. Hanson,
V. S. Paes-Takahashi,
P. Vieira
journal of nematology, Volume 51 , 1–16
research-article | 18-March-2020
spherical, filled with sperm. Vagina is straight, vulva is closed, and vulval lips do not project above body contour. The ventral side of postvulval annuli is inverted, in majority of individuals. Anus is indistinct and located on the next annuli posterior to vulva. Tail is short and conoid, longitudinal striations are more prominent on the terminal annulus that gives the appearance of forked or branched terminus.
Figure 4:
Scanning electron microscopy of Nothocriconemoides hangzhouensis n. sp. Female
Munawar Maria,
Wentao Miao,
Ruihang Cai,
Pablo Castillo,
Jingwu Zheng
Journal of Nematology, Volume 52 , 1–14
research-article | 16-April-2020
. sp. Male. A: entire body; pharyngeal regions, arrow pointing on the excretory pore (exp); C: tail with bursa; D: gonad; E, F: tail in ventral view; G, I: male tail arrows pointing on phasmid (ph) (Scale bars = A = 100 μm; B-I = 10 μm).
Figure 4:
Scanning electron microscopy of Geocenamus chengi n. sp. A: anterior region; B-C: en face view; D-F: vulval regions; G, H: female tail regions arrows pointing anus (a); I: anal area at higher resolution, arrow pointing on the position of anus; J
Munawar Maria,
Wentao Miao,
Pablo Castillo,
Jingwu Zheng
Journal of Nematology, Volume 52 , 1–13
Research Article | 15-February-2020
. Under a constant flow of Argon gas (200mL/min), the substrates along with a mixture of ZnO and graphite powder are placed in a modified horizontal furnace which uses a 2.54 cm internally mounted quartz tube and are exposed to high temperature (527°C-1000°C) for different growth times. This reaction causes the formation of ZnO nanowires on the substrates. scanning electron microscopy is used to measure the size of the nanowires.
Sushma Nandhyala,
Michael Haji-Sheikh,
Martin Kocanda,
Suma Rajashankar
International Journal on Smart Sensing and Intelligent Systems, Volume 7 , ISSUE 5, 1–5
Research Article | 17-October-2018
juveniles, males and females were carried out by light compound and scanning electron microscopy. Gross morphology and measurements were found consistent with the original description of M. indica infecting citrus by Whitehead (1968). The neem population was found to infect and reproduce on citrus. Additionally, evolutionary relationship was deduced by Maximum likelihood method using ITS rRNA, D2D3 expansion segment of 28S rRNA and mitochondrial COI sequences. Phylogenetic analyses based on these
Victor Phani,
Satyapal Bishnoi,
Amita Sharma,
Keith G. Davies,
Uma Rao
Journal of Nematology, Volume 50 , ISSUE 3, 387–398
Research Article | 03-December-2018
magnetic resonance spectral data analysis. The LC50 values against B. xylophilus in 72 hr were 489.17, 406.74, 430.08, and 435.66 μM, respectively. These compounds also altered the smooth morphology of the B. xylophilus exoskeleton to a rough and pitted appearance as visualized by electron microscopy. The coumarins 1-4 possessed significant acetylcholinesterase inhibitory activities but had negligible effects on amylase and cellulase. This research provides additional clues to the nematotoxic mechanism
Qun-Qun Guo,
Gui-Cai Du,
Yong-Xing Li,
Chen-Yan Liang,
Chao Wang,
Ya-Nan Zhang,
Rong-Gui Li
Journal of Nematology, Volume 50 , ISSUE 4, 559–568
Article | 01-March-2012
ZnO nano-particles have been synthesized by simple chemical route using a starting solution consisting of zinc acetate and citric acid as a surfactant agent. The structural properties of the prepared ZnO nano-particles annealed at different temperatures have been characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM) analyses. The XRD patterns show ZnO-wurtize phase in the nano-powders, and size of crystals increases by increasing the annealing temperatures. The TEM
Sarika D. Shinde,
G. E. Patil,
D. D. Kajale,
V. G. Wagh,
V. B. Gaikwad,
G. H. Jain
International Journal on Smart Sensing and Intelligent Systems, Volume 5 , ISSUE 1, 277–294
research-article | 30-November-2019
known about these properties in H. diversa (Liu and Yu, 2002; Uematsu et al., 2015, 2016). Liu and Yu (2002) observed the morphology of H. diversa using scanning electron microscopy, and Uematsu et al. (2015) described three weed species parasitized by H. diversa in Indian lotus fields. Light and electron microscopy analysis of Indian lotus rootlets revealed all stages of the H. diversa lifecycle, from the egg to the juvenile and adult forms (Uematsu et al., 2016). However, the parasitic behavior of
Shigeru Uematsu,
Tetsuo Yabu,
Mitsuyoshi Yao,
Takayuki Kurihara,
Hironori Koga
Journal of Nematology, Volume 52 , 1–9
research-article | 30-November-2019
nodule-like gall were elucidated by light, scanning, and transmission electron microscopy (Eisenback and Dodge, 2012; Dodge, 2014). The galls induced by M. kikuyensis are unique and more complex than those caused by most root-knot nematode species. The vascular tissues that supply the giant cells with nutrients occur at a right angle to the vascular cylinder in the main root. Unlike most species of root-knot nematodes, feeding cells of M. kikuyensis appear to be formed by the dissolution of cell
J. D. Eisenback,
P. Vieira
Journal of Nematology, Volume 52 , 1–13
research-article | 30-November-2020
:
Scanning electron microscopy (SEM) of Gongylonema sp. (1) Anterior portion of a specimen L3 without cuticular bosses and highlighting the lateral ala (La). (2) Central view of the cephalic end, highlighting the amphids (Am), 4 cervical papillae (Cp), 4 internal papillae (Ip), 6 inner lateral papilla (ILp), peribuccal collar (Pc) and cuticular plates on the margins of the buccal opening. (3) Detail of lateral alae (La) and excretory pore (Ep). (4) Details of excretory pore (Ep). (5) Posterior portion of
Daniel Bravo-Barriga,
Manuel Martín-Pérez,
Jorge M. Lobo,
Ricardo Parreira,
Juan Enrique Pérez-Martín,
Eva Frontera
Journal of Nematology, Volume 53 , 1–9
research-article | 24-April-2020
as template to redraw in CorelDRAW® software version 2018.
Scanning electron microscopy (SEM)
For SEM, the nematodes mounted on permanent slides were cleaned and hydrated in distilled water, dehydrated in a graded ethanol-acetone series, critical point dried, coated with gold, and observed with a Zeiss Merlin microscope (5 kV) (Zeiss, Oberkochen, Germany) (Abolafia, 2015).
DNA extraction, PCR reaction, and gel electrophoresis
DNA extraction and PCR conditions followed Rashidifard et al. (2019
Milad Rashidifard,
Gerhard Du Preez,
Joaquín Abolafia,
Majid Pedram
Journal of Nematology, Volume 52 , 1–10
research-article | 22-February-2021
Kenya. The potential importance of this nematode as a pest of finger millet was evidenced by its dense population in the soil sample and its presence observed in the finger millet root system (revealed by the fuchsin staining method). Nematode characterization was carried out based on morphological information obtained from light microscopy (LM) and scanning electron microscopy (SEM) studies. Illustrations, morphometrics, and molecular information of ITS, 18S, and 28S of rDNA and COI of mtDNA are
Phougeishangbam Rolish Singh,
Gerrit Karssen,
Kelvin Gitau,
Cecilia Wanjau,
Marjolein Couvreur,
Njira Njira Pili,
Godelieve Gheysen,
Wim Bert
Journal of Nematology, Volume 53 , 1–14
research-article | 11-March-2021
drawing tube (camera lucida) attached to an Omax microscope (China). Pictures were taken with a Nikon Eclipse 80i light microscope provided with differential interference contrast optics (DIC) and a Nikon Digital Sight DS-U1 camera (Nikon, Tokyo, Japan). Micrographs were edited using Adobe® Photoshop® CS. The terminology used for the morphology of stoma follows the proposals by Baldwin et al. (2004).
Scanning electron microscopy (SEM)
Specimens preserved in glycerine were selected for observation
Ebrahim Shokoohi
journal of nematology, Volume 53 , 1–13
Research Article | 03-September-2018
was made using light and scanning electron microscopy of the second stage juveniles, females, males and cysts, and the host range study, was performed with nine different plants from five families. The molecular identification was made by sequencing and analysing the ITS rRNA and partial COI genes. It was shown that using presently available molecular tools it is not possible to make an accurate differentiation of H. carotae from H. cruciferae. The host range test allowed to distinguish these
Ilia Mariana Escobar-Avila,
Edgar Óliver López-Villegas,
Sergei A. Subbotin,
Alejandro Tovar-Soto
Journal of Nematology, Volume 50 , ISSUE 2, 229–242
Research paper | 10-October-2018
Bruchpilot (BRP), a presynaptic scaffolding protein that is crucial for neurotransmission in all type of synapses in Drosophila. In addition, other proteins that are involved in synaptic transmission; Rab5, Syntaxin and Wishful Thinking were also decreased in abundance in mutants, except Synaptotagmin. Transmission electron microscopy (TEM) also confirmed less and abnormal synaptic vesicles at tetrad synapses in the visual system of PINK1 mutants. The lower level of BRP and longer day sleep observed was
Bartosz Doktór,
Milena Damulewicz,
Wojciech Krzeptowski,
Barbara Bednarczyk,
Elżbieta Pyza
Acta Neurobiologiae Experimentalis, Volume 78 , ISSUE 3, 231–241
research-article | 30-November-2018
.
Scanning electron microscopy (SEM)
Specimens preserved in glycerine were selected for observation under SEM according to Abolafia (2015). The nematodes were hydrated in distilled water, dehydrated in a graded ethanol-acetone series, critical point dried, coated with gold, and observed with a Zeiss Merlin microscope (5 kV) (Zeiss, Oberkochen, Germany).
Statistical analysis
Principal component analysis and the correlation of morphometric data using the Pearson method was done by XLSTAT (Addinsoft, 2007
Ebrahim Shokoohi,
Joaquín Abolafia,
Phatu William Mashela,
Nafiseh Divsalar
Journal of Nematology, Volume 51 , 1–26
research-article | 30-November-2019
.
Scanning electron microscopy (SEM)
Specimens preserved in glycerin were selected for observation according to Abolafia (2015). They were hydrated in distilled water, dehydrated in a graded mixture of ethanol-acetone series, critical point-dried with liquid carbon dioxide, and coated with gold. The mounts were examined with a Zeiss Merlin microscope (5 kV).
DNA extraction, PCR and sequencing
For molecular analyses, single female specimens were picked out, examined in a drop of distilled water on a
Sedighe Azimi,
Joaquín Abolafia,
Majid Pedram
Journal of Nematology, Volume 52 , 1–19
Research Article | 31-May-2018
In a search for an entomopathogenic nematode to control cranberry insect pests, three Oscheius populations (Rhabditidae) were recovered through the Galleria-bait method from one sample taken in a wild cranberry marsh in Jackson County, Wisconsin, USA. Morphological studies with light microscopy and scanning electron microscopy, as well as molecular analyses of the near-full-length small subunit rDNA gene, D2/D3 expansion segments of the large subunit rDNA gene, internal transcribed spacer, and
Weimin Ye,
Shane Foye,
Ann E. MacGuidwin,
Shawn Steffan
Journal of Nematology, Volume 50 , ISSUE 1, 9–26
research-article | 30-November-2018
Pingelly with typical P. curvicauda features were fixed in 4% formaldehyde solution and sent to an expert taxonomist in the UK for further characterization. The detailed morphometric measurements obtained were compared with those taken in Australia.
Preparation of nematode samples for scanning electron microscopy
Scanning electron microscopy (SEM) was used to further characterize P. curvicauda. To do this, single nematodes were fixed in 3% glutaraldehyde in 0.025 M phosphate buffer (pH 7.0) overnight
Farhana Begum,
John Fosu-Nyarko,
Shashi Sharma,
Bill Macleod,
Sarah Collins,
Michael G. K. Jones
Journal of Nematology, Volume 51 , 1–15
research-article | 30-November-2020
Paratylechus beltsvillensis n. sp. The objective of this study was also to describe this new species using light (LM) and scanning electron microscopy (SEM) and provide its molecular characterization.
Materials and methods
Nematode samples
In September and October of 2020, few soil samples were collected in the Little Paint Branch Park, Beltsville, Prince George’s County, Maryland, USA And sent to the Plant Pest Diagnostic Center, California Department of Food and Agriculture, Sacramento, CA and part of
Mihail R. Kantor,
Zafar A. Handoo,
Sergei A. Subbotin,
Gary R. Bauchan,
Joseph D. Mowery
Journal of Nematology, Volume 53 , 1–10
Research paper | 25-July-2017
remodeling of brain parenchyma, including the presence of multiple cell types of immature phenotypes. The latter, as shown by a variety of light and electron microscopy techniques, included endothelial cell precursors as well as nestin-positive immature neural cells of astrocytic or non-glial characteristics. However, there was no evidence of in situ neurogenesis or a considerable migration of cells from SVZ. The centers of the said repair processes were capillary blood vessels connected with basal
Dorota Sulejczak,
Stanisław J. Chrapusta,
Wojciech Kozłowski,
Małgorzata Frontczak-Baniewicz
Acta Neurobiologiae Experimentalis, Volume 76 , ISSUE 2, 125–141
Original Paper | 28-June-2017
An Egyptian, plant pathogenic Fusarium solani isolate was grown on cobalt concentrations of 0, 50, 200, 500, 800, and 1000 ppm. The isolate survived concentrations up to 800 ppm, however failed to grow at 1000 ppm. Morphology and elemental analysis of the isolate under the investigated Co concentrations were studied using Scanning electron microscopy (SEM) and energy dispersive X-ray microanalysis (EDX). The isolate reserved its morphology up to a concentration of 200 ppm. Morphological
Farrag M. Rasha
Polish Journal of Microbiology, Volume 66 , ISSUE 2, 235–243
Research Article | 26-September-2018
dissected to investigate the presence of nematode larvae. Thirty fish (42.9%) of the total were parasitized by nematode larvae which were morphologically identified as Anisakis spp. Type II (L3) according to light and scanning electron microscopy. The pathogenic potential of oral inoculation of fresh, frozen, and thermally treated larvae into Wistar rats was elucidated by histological examination of their thymus and spleen. Results obtained indicated that neither cooling nor freezing of the parasite
KAREEM MORSY,
ABEER MAHMOUD BADR,
FATHY ABDEL-GHAFFAR,
SOMAYA EL DEEB,
SAMAR EBEAD
Journal of Nematology, Volume 49 , ISSUE 4, 427–436
Research paper | 06-February-2018
-lesion. Anterograde tracing analyses exhibited a progressive decline of TCA fiber density in barrel field for 6 months post-lesion. Due to the abrupt decrease of TCA fiber density at 3 days after compression, we further used electron microscopy to investigate the ultrastructure of TCA fibers at this time. Some TCA fiber terminal profiles with dissolved or darkened mitochondria and fewer synaptic vesicles were distorted and broken. Furthermore, the disruption of mitochondria and myelin sheath was
Tzu-Yin Yeh,
Guo-Fang Tseng,
Chi-Yu Tseng,
Yung-Hsin Huang,
Pei-Hsin Liu
Acta Neurobiologiae Experimentalis, Volume 77 , ISSUE 1, 1–17
research-article | 30-November-2020
E600 light microscope. Photomicrographs were taken using an Olympus DP72 digital camera attached to an Olympus BX51 microscope equipped with differential interference contrast. Drawings were made using a drawing tube attached to the microscope and were redrawn using the CorelDRAW® software version 2017.
Scanning electron microscopy
For the scanning electron microscopy, specimens preserved in glycerine were selected for observation under SEM according to the Abolafia’s (2015) protocol. The
Farahnaz Jahanshahi Afshar,
Milad Rashidifard,
Joaquín Abolafia,
Miloslav Zouhar,
Hendrika Fourie,
Majid Pedram
Journal Of Nematology, Volume 53 , 1–14