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mini-review | 05-December-2019

Antibiotic Resistance Among Uropathogenic Escherichia coli

recommended for the treatment of UTIs. The resistance to antibiotics of UPEC isolated in different regions of the world (European countries, North America, Asia, and some countries of Africa) was compared. The increase of resistance to fluoroquinolones, which was significantly higher in developing countries than in developed countries, has been revealed. Also, the resistance mechanisms identified in these pathogens were discussed. Escherichia coli as a main etiological agent of UTIs The bacteria

BARBARA KOT

Polish Journal of Microbiology, Volume 68 , ISSUE 4, 403–415

Original Paper | 30-March-2017

Expression of the Fluoroquinolones Efflux Pump Genes acrA and mdfA in Urinary Escherichia coli Isolates

Escherichia coli is one of the most frequent causes of urinary tract infections. Efflux system overexpression is reported to contribute to E. coli resistance to several antibiotics. Our aim in this study was to investigate the relation between antibiotic resistance and the expres­sion of the efflux pump genes acrA and mdfA in E. coli by real-time reverse transcription-PCR. We tested the in vitro susceptibilities to 12 antibiotics in 28 clinical isolates of E. coli obtained from urine

Sarah M. Abdelhamid, Rania R. Abozahra

Polish Journal of Microbiology, Volume 66 , ISSUE 1, 25–30

Original Paper | 26-August-2016

Molecular Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated in Poland

Shiga toxin-producing Escherichia coli (STEC) strains also called verotoxin-producing E. coli (VTEC) represent one of the most important groups of food-borne pathogens that can cause several human diseases such as hemorrhagic colitis (HC) and hemolytic – uremic syndrome (HUS) worldwide. The ability of STEC strains to cause disease is associated with the presence of wide range of identified and putativevirulence factors including those encoding Shiga toxin. In this study, we examined the

Aleksandra Januszkiewicz, Waldemar Rastawicki

Polish Journal of Microbiology, Volume 65 , ISSUE 3, 261–269

Article | 24-March-2021

SANDBOXES AS A POTENTIAL SOURCE OF DANGEROUS DRUG-RESISTANT ESCHERICHIA COLI AND STAPHYLOCOCCUS AUREUS STRAINS

pasożytami. Panujące w piaskownicach warunki: podwyższona wilgotność oraz temperatura pomiędzy 22–30°C, sprzyjają wzrostowi bakterii i grzybów w tym dermatofitów. Wśród tych mikroorganizmów mogą być patogeny wywołujące grzybice, które nie tylko zmieniają powierzchnię skóry wywołując dyskomfort ale również są trudne w leczeniu. Coraz częściej w miejscach użyteczności publicznej pojawiają się mikroorganizmy, których obecność wydaje się być niepokojąca. Przykładem są bakterie Escherichia coli, których

Edyta Mazur, Maria Jolanta Chmiel

Postępy Mikrobiologii - Advancements of Microbiology, Volume 60 , ISSUE 1, 77–89

short-communication | 18-February-2020

Sub-lethal Concentrations of Phytochemicals (Carvacrol and Oregano) Select for Reduced Susceptibility Mutants of Escherichia coli O23:H52

AFNAN A. AL-MNASER, MARTIN J. WOODWARD

Polish Journal of Microbiology, Volume 69 , ISSUE 1, 121–125

research-article | 30-November-2018

THE USAGE OF ULTRASOUNDS TO DISINTEGRATE ESCHERICHIA COLI BACTERIA CONTAINED IN TREATED WASTEWATER

, and parasitic protists (protozoa) and worms react in a different way to the disinfection process. Classical disinfection of sewage and by-products that arise during purification processes can be carried out by physical and chemical methods [10]. One of these methods is ultrasonic disintegration. 3. METHODOLOGY OF LABORATORY TESTS The aim of the experiments was to determine the effect of low-frequency ultra-sound (20 and 40 kHz) on the survival of Escherichia coli bacteria present in ultrapure

Eliza HAWRYLIK

Architecture, Civil Engineering, Environment, Volume 12 , ISSUE 3, 131–136

Research Article | 15-February-2020

Detection of Pathogenic Bacteria in Aqueous Media: Assessing the Potential of Real-Time Electromagnetic Wave Sensing

This paper reports on the capabilities of a novel electromagnetic wave sensing method to detect and identify the presence of various pathogenic bacteria in aqueous media. In particular, the change in the electromagnetic wave signal in microwave frequency range is used as an indicator of bacteria presence. The assessment was conducted by recording reflected signal spectra when the sensor was in contact with deionised water, Escherichia coli, sterile nutrient broth and Pseudomonas aeruginosa

I. Nakouti, O. Korostynska, A. Mason, A. I. Al-Shamma’a

International Journal on Smart Sensing and Intelligent Systems, Volume 7 , ISSUE 5, 1–6

Article | 01-March-2015

CHARACTERIZATION OF A NEEDLE-TYPE GIANT MAGNETORESISTANCE SENSOR FOR DETECTION OF ESCHERICHIA COLI’S MAGNETIC MARKER

, designed for the identification and quantification of Escherichia coli O157:H7 bacteria covered by superparamagnetic beads, Dynabeads® MAX E.coli O157. The sensor characteristics, measurement system setup and the properties of the magnetic marker solution are discussed in detail.

Hamidreza Shirzadfar, Mustapha Nadi, Djilali Kourtiche, Sotoshi Yamada, Payman Shahabi

International Journal on Smart Sensing and Intelligent Systems, Volume 8 , ISSUE 1, 220–234

Mini Review | 30-June-2018

Non-antibiotics, Efflux Pumps and Drug Resistance of Gram-negative Rods

observed. It has also been revealed that depending on the concentration, salicylate induces expression of multi-drug resistance efflux pumps in Escherichia coli, Salmonella enterica subsp. enterica serotype Typhimurium, and Burkholderia cenocepacia. However, salicylate does not affect the expression of the resistance-nodulation-division efflux systems in Stenotrophomonas maltophilia and Acinetobacter baumannii. Most importantly, there were no effects of medicinal products containing some non-antibiotic

AGNIESZKA EWA LAUDY

Polish Journal of Microbiology, Volume 67 , ISSUE 2, 129–135

Original Paper | 10-December-2018

Microbiology and Drug Resistance of Pathogens in Patients Hospitalized at the Nephrology Department in the South of Poland

Abstract A retrospective study was conducted among 498 patients with urinary tract infections (UTI) referred to our department from January 2013 to December 2015. This study was performed to evaluate the etiology of UTI and the antibiotic susceptibility profile of Escherichia coli (E. coli) as the main etiological factor in different age groups. Urine samples were examined using standard microbiological methods. Three hundred sixty-three samples (72.9%) were identified as E. coli, of which 29

MIKOŁAJ MICHNO, ANTONI SYDOR, MARTA WAŁASZEK, WŁADYSŁAW SUŁOWICZ

Polish Journal of Microbiology, Volume 67 , ISSUE 4, 517–524

Original Paper | 04-September-2018

Improved Production of Recombinant Human β-NGF in Escherichia coli – a Bioreactor Scale Study

Human nerve growth factor β (β-NGF) is considered a major therapeutic agent for treatment of neurodegenerative diseases. We have previously reported the optimized conditions for β-NGF overproduction in Escherichia coli in a shake-flask culture. In this study the optimal %DO (dissolved oxygen) and post induction temperature values for improved production of β-NGF were found in the bioreactor scale using response surface methodology (RSM) as the most common statistical method. Also, for further

ZAHRA HAJIHASSAN, POURIA GHOLAMI TILKO, SEYEDEH MAHDIEH SADAT

Polish Journal of Microbiology, Volume 67 , ISSUE 3, 355–363

Article | 23-March-2020

THE ROLE AND IMPORTANCE OF SELECTED VIRULENCE FACTORS DETERMINING THE PATHOGENICITY OF UROPATHOGENIC ESCHERICHIA COLI STRAINS

1. Wstęp Pozajelitowe patogenne szczepy Escherichia coli ExPEC (extraintestinal patogenic E. coli) charakteryzują się różnorodnymi genotypami, jak i fenotypami, a w ich obrębie wyróżnia się, m.in. grupę uropatogennych szczepów Escherichia coli (uropathogenic E. coli, UPEC) odpowiedzialnych za 75–95% przypadków zakażeń układu moczowego (ZUM), zarówno u dzieci, jak i osób dorosłych [4, 6, 10, 33, 34]. UPEC mogą kolonizować nabłonek układu moczowego człowieka, lecz o ich chorobotwórczości i

Sylwia Joanna Chmielewska, Katarzyna Leszczyńska

Postępy Mikrobiologii - Advancements of Microbiology, Volume 59 , ISSUE 1, 25–37

Original Paper | 28-June-2017

Genetic Characterization of a Novel Composite Transposon Carrying armA and aac(6)-Ib Genes in an Escherichia coli Isolate from Egypt

surrounding the armA gene. Out of 123 isolates, 66 (53.66%) were resistant to at least oneaminoglycoside antibiotic. Only one Escherichia coli isolate (E9ECMO) which was totally resistant to all tested aminoglycosides, was confirmed to have the armA gene in association with blaTEM-1, blaCTX-M-15, blaCTX-M-14 and aac(6)-Ib genes. The armA gene was found to be carried on a large A/C plasmid. Genetic mapping of the armA surrounding region revealed, for the first time, the association of armA with aac(6)-Ib

Mona T. Kashef, Omneya M. Helmy

Polish Journal of Microbiology, Volume 66 , ISSUE 2, 163–169

Article | 30-November-2018

DIFFUSELY ADHERING ESCHERICHIA COLI

1. Introduction Escherichia coli is a facultative anaerobe that is part of the human colon’s microbiota. This organism colonizes the intestines of newborns as early as in the first hours of their life. Most E. coli rods are harmless and its presence is limited to the digestive tract. Nevertheless, in weakened people, patients suffering from immunosuppression and as a result of damage to the intestinal epithelial layer, even these non-pathogenic strains of E. coli may cause extraintestinal

Michał Turniak, Beata Sobieszczańska

Postępy Mikrobiologii - Advancements of Microbiology, Volume 58 , ISSUE 2, 143–152

Article | 04-January-2021

GENEROWANIE MOSTKÓW DISIARCZKOWYCH W BIAŁKACH – RÓŻNORODNOŚĆ STRUKTURALNA I FUNKCJONALNA BIAŁEK DSBA

(mikrobiologicznych, biochemicznych, biofizycznych, bioinformatycznych czy proteomicznych) zaowocowało w ostatnich latach szczegółową charakterystyką funkcjonowania sieci Dsb modelowej bakterii Escherichia coli (EcDsb). Dane te przedstawia wiele znakomitych publikacji przeglądowych. Ostatnio opublikowane to między innymi [3, 40, 43]. Doskonalenie metod sekwencjonowania materiału genetycznego ujawniło różnorodność systemów Dsb w świecie mikroorganizmów. Różnice dotyczą liczby białek Dsb konkretnej kate gorii, ich

Anna Marta Banaś, Anna Petrykowska, Elżbieta Katarzyna Jagusztyn-Krynicka

Postępy Mikrobiologii - Advancements of Microbiology, Volume 59 , ISSUE 4, 345–355

Article | 05-October-2019

THE ROLE OF THE STRINGENT COMPONENTS IN THE REGULATION OF VIRULENCE

dokładnie dla modelowych bakterii, Gram-ujemnych i Gram-dodatnich. U Escherichia coli poziom cztero- i pięciofosforanu guanozyny kontrolowany jest przez aktywność produktów genów relA i spoT. ppGpp i pppGpp mogą być syntetyzowane przez białka RelA i SpoT, a powstają odpowiednio z GDP lub GTP oraz ATP. Z kolei dwufunkcyjne SpoT ma również zdolność hydrolizy ppGpp do GDP i pirofosforanu (PPi) oraz pppGpp do GTP i PPi. Większość Gammaproteobacteria i część Betaproteobacteria, takich jak E. coli, Salmonella

Klaudyna Krause, Klaudia Milewska, Agnieszka Szalewska-Pałasz

Postępy Mikrobiologii - Advancements of Microbiology, Volume 58 , ISSUE 3, 247–258

Short Communication | 30-March-2017

Vital Staining of Bacteria by Sunset Yellow Pigment

In this study, we describe a method for discriminating pathogenic bacteria with a dye. First, we determined that among several colours tested, the sunset yellow pigment easily coloured Escherichia coli bacteria yellow. Next, we demonstrated that E. coli O157:H7, Shigella flexneri O301, Staphylococcus aureus and Bacillus subtilis could all be well marked by sunset yellow pigment. Finally, we performed bacterial viability assays and found there was no effect on bacterial growth when in co-culture

Peng Gao, Chengming Sun, Yuxia Li, Xiaohua Zou, Xun Wu, Yan Ling, Caifu Luan, Huipeng Chen

Polish Journal of Microbiology, Volume 66 , ISSUE 1, 113–117

short-communication | 05-December-2019

Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation

ASHVEEN BAINS, JAMES W. WILSON

Polish Journal of Microbiology, Volume 68 , ISSUE 4, 559–563

short-communication | 07-June-2021

Occurrence of Beta-Lactamases in Colistin-Resistant Enterobacterales Strains in Poland – a Pilot Study

been many reports about plasmid resistance to colistin among strains isolated from human infections (Kluytmans 2017; Elbediwi et al. 2019). In Poland, the first Escherichia coli strain with the mcr-1 gene was described in 2016 (Izdebski et al. 2016). However, we do not have more information about the presence of mcr genes in Poland. As β-lactam antibiotics are “first-line” drugs in the treatment of infections caused by Enterobacterales, the susceptibility of strains to this group of antimicrobial

ELŻBIETA M. STEFANIUK, ALEKSANDRA KOZIŃSKA, IZABELA WAŚKO, ANNA BARANIAK, STEFAN TYSKI

Polish Journal of Microbiology, Volume 70 , ISSUE 2, 283–288

Short Communication | 04-December-2017

Inhibition of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria by a Photoactivated Porphyrin

The authors studied the in vitro antibacterial activity of the photo-activated porphyrin meso-tri(N-methyl-pyridyl), mono(N-tetradecyl-pyridyl)porphine (C14) against four multidrug-resistant bacteria: Staphylococcus aureus, Enterococcus faecalis (Gram-positive), Escherichia coli, Pseudomonas aeruginosa (Gram-negative). Using 10 μg/ml of porphyrin and 60 sec irradiation we observed the remarkable suscep­tibility of S. aureus and E. faecalis to treatment while, under the same conditions, E

Moreno Bondi, Anna Mazzini, Simona De Niederhäusern, Ramona Iseppi, Patrizia Messi

Polish Journal of Microbiology, Volume 66 , ISSUE 4, 533–536

Original Paper | 04-December-2017

Characterisation of Antimicrobial Properties of Extracts of Selected Medicinal Plants

antimicrobial activity against the tested strains was demonstrated by black elder (Sambucus nigra L.), black locust (Robinia pseudoacacia L.) and lingonberry (Vaccinium vitis-idaea L.) extracts. The study showed the diverse morphological activity of specific parts of elderberry and quince, which is the effect of different polyphenolic profile of these plants. The yeast Saccharomyces cerevisiae, Escherichia coli, Pseudomonas putida and Bacillus subtilis showed the highest sensitivity to the effect of

Monika Cioch, Paweł Satora, Magdalena Skotniczy, Dorota Semik-Szczurak, Tomasz Tarko

Polish Journal of Microbiology, Volume 66 , ISSUE 4, 463–472

Research Article | 22-May-2019

STRATEGIES FOR THE ANALYSIS OF THIOLOXIDORDUCTASES

Bacterial Dsb (disulfide bond) enzymes are involved in the oxidative folding of many proteins, through the formation of disulfide bonds between thiol groups of cysteine residues. This process is critical for the correct folding and structural stability of many secreted and membrane proteins. The rapidly expanding number of sequenced bacterial genomes has revealed the enormous diversity among bacterial Dsb systems. While the Escherichia coli oxidative protein folding has been studied in great

Elżbieta Katarzyna Jagusztyn-Krynicka, Anna Marta Banaś, Magdalena Joanna Grzeszczuk

Postępy Mikrobiologii - Advancements of Microbiology, Volume 56 , ISSUE 3, 326–334

Review | 21-April-2020

Review: Cromer and DAF: role in health and disease

the critical C3 convertase step. By this mechanism,DAF acts to protect autologous cells and tissues from complement-mediated damage and hence can play a role in preventing or modulating autoimmune disease and inflammation. The use of recombinant DAF as a therapeutic agent in autoimmunity and inflammation, and of DAF transgenic animals in xenotransplantation, is being actively investigated. Additionally, DAF serves as a receptor for certain strains of Escherichia coli and certain types of

Douglas M. Lublin

Immunohematology, Volume 21 , ISSUE 2, 39–47

research-article | 30-November-2018

Active and inactive forms of biotin synthase occur in Heterodera glycines

extracted from the overnight culture using a QIAprep Spin Miniprep Kit (Qiagen) and the resulting plasmids were sequenced for verification at the University of Illinois Roy J. Carver Biotechnology Center. Complementation of ΔbioB Escherichia coli with avirulent and virulent alleles of HgBioB A kanamycin-resistant E. coli vitamin B7 auxotroph (K-12 Keio Collection: JW0758-1) was obtained from the Coli Genetic Stock Center at Yale University and used to prepare electrocompetent cells as described by

Khee Man Kwon, Sadia Bekal, Leslie L. Domier, Kris N. Lambert

Journal of Nematology, Volume 51 , 1–12

original-paper | 05-December-2019

New Look on Antifungal Activity of Silver Nanoparticles (AgNPs)

do not exhibit toxicity for mammalian cells (Milic et al. 2015; Zhang et al. 2016a). However, some cytostatic and anti-cancer activity is reported at these concentrations (AshaRani et al. 2009). Antibacterial activity of AgNPs is rather well documented in the literature, mainly against the following species: Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli (Yoon et al. 2007; Li et al. 2010; Guzman et al. 2012, Kanawaria et al. 2018). Some information

BARBARA ŻAROWSKA, TOMASZ KOŹLECKI, MICHAŁ PIEGZA, KATARZYNA JAROS-KOŹLECKA, MAŁGORZATA ROBAK

Polish Journal of Microbiology, Volume 68 , ISSUE 4, 515–525

Original Paper | 28-June-2017

In vitro Interaction between Fumonisin B1 and the Intestinal Microflora of Pigs

The caecal chyme of pigs was incubated anaerobically in McDougall buffer with and without fumonisin B1 (5 μg/ml) for 0, 24 and 48 h. The plate count agar technique was applied for enumerating the amount of bacteria including aerobic, anaerobic bacteria, coliform, Escherichia coli and Lactobacillus sp. The quantitative polymerase chain reaction was also performed to estimate the number of copies of the total bacteria, Lactobacillus, Bacteroides and Prevotella. No significant differences in

Huu Anh Dang, Attila Zsolnai, Melinda Kovacs, István Bors, András Bónai, Brigitta Bóta, Judit Szabó-Fodor

Polish Journal of Microbiology, Volume 66 , ISSUE 2, 245–250

Original Research | 04-September-2018

Optimization of Mixed Solid-state Fermentation of Soybean Meal by Lactobacillus Species and Clostridium butyricum

Staphylococcus aureus and Escherichia coli. These results together suggest that supplementation of both proteases and probiotics in SSF improves the nutritional value of fermented soybean meal and this is suitable as a protein source in animal feed.

LI-WEN SU, YEONG-HSIANG CHENG, FELIX SHIH-HSIANG HSIAO, JIN-CHENG HAN, YU-HSIANG YU

Polish Journal of Microbiology, Volume 67 , ISSUE 3, 297–305

original-paper | 28-June-2019

Patterns of Drug-Resistant Bacteria in a General Hospital, China, 2011–2016

a more complete picture of bacterial infections and to help clinicians and decision-making departments undertake the proper decisions for patients and antibiotic use. Experimental Materials and Methods Based on the data from a general hospital in Zhengzhou, Henan province, China from 2011 to 2016, five dominant bacteria (Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Acinetobacter baumannii) were investigated in this study. The antibiotic

TINGTING MAO, HUIJUAN ZHAI, GUANGCAI DUAN, HAIYAN YANG

Polish Journal of Microbiology, Volume 68 , ISSUE 2, 225–232

original-paper | 23-November-2020

Biofilm Production Potential of Salmonella Serovars Isolated from Chickens in North West Province, South Africa

allow for a numerical evaluation of the ability of bacteria to form biofilms. In this study, the quantitative assays were adopted based on its accuracy, reliability, and potential to enable precise quantification instruments. Biofilm forming pathogens (Salmonella Typhimurium, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis) have been isolated in food and food processing plants in developed and developing countries (Dourou et al. 2011; Cook et al. 2012

STEPHEN ABIOLA AKINOLA, MPINDA EDOAURD TSHIMPAMBA, MULUNDA MWANZA, COLLINS NJIE ATEBA

Polish Journal of Microbiology, Volume 69 , ISSUE 4, 427–439

Research Article | 24-May-2019

TYPE VB AND VI SECRETION SYSTEMS AS COMPETITION AGENTS OF GRAM-NEGATIVE BACTERIA

phenomenon. A contact-dependent growth inhibition (CDI), primarily found in Escherichia coli strains, utilizes CdiB/CdiA protein of type Vb secretion system, described also as two-partner secretion (TPS) system, to inhibit growth of non-kin strains, where cell contact is required. Presence of an intracellular small immunity protein (CdiI) protects E. coli cells from autoinhibition. Other bacterial competition system, involved mainly in the nodulation process of Rhizobium leguminosarum bv. Trifolii strain

Dawid Gmiter, Grzegorz Czerwonka, Wiesław Kaca

Postępy Mikrobiologii - Advancements of Microbiology, Volume 57 , ISSUE 4, 360–373

Article | 30-November-2018

ANTIBIOTIC RESISTANCE OF BACTERIA A GROWING THREAT FOR ANIMALS AND PUBLIC HEALTH

%). Moreover, 55–64% of the isolates demonstrated cross-resistance to ciprofloxacin and nalidixic acid. Table II Percentage of commensal Escherichia coli strains resistant to selected antimicrobials, isolated from fattening pigs Country/average in EU Number of strains tested Percentage of resistant strains Gentamicin Chloramphenicol Ampicillin Cefotaxime Ceftazidime Ciprofloxacin Colistine Sulfamethoxazole Tetracicline Spain 170 4.7 35.3 77.1 2.9 2.9 44.7 0.6 63.5 88.8 Portugal 142

Marian Binek, Magdalena Kizerwetter-Świda, Magdalena Rzewuska, Dorota Chrobak-Chmiel, Agnieszka Sałamaszyńska-Guz

Postępy Mikrobiologii - Advancements of Microbiology, Volume 58 , ISSUE 3, 259–270

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