mini-review | 05-December-2019
recommended for the treatment of UTIs. The resistance to antibiotics of UPEC isolated in different regions of the world (European countries, North America, Asia, and some countries of Africa) was compared. The increase of resistance to fluoroquinolones, which was significantly higher in developing countries than in developed countries, has been revealed. Also, the resistance mechanisms identified in these pathogens were discussed.
Escherichia coli as a main etiological agent of UTIs
The bacteria
BARBARA KOT
Polish Journal of Microbiology, Volume 68 , ISSUE 4, 403–415
Original Paper | 30-March-2017
Escherichia coli is one of the most frequent causes of urinary tract infections. Efflux system overexpression is reported to contribute to E. coli resistance to several antibiotics. Our aim in this study was to investigate the relation between antibiotic resistance and the expression of the efflux pump genes acrA and mdfA in E. coli by real-time reverse transcription-PCR. We tested the in vitro susceptibilities to 12 antibiotics in 28 clinical isolates of E. coli obtained from urine
Sarah M. Abdelhamid,
Rania R. Abozahra
Polish Journal of Microbiology, Volume 66 , ISSUE 1, 25–30
Original Paper | 26-August-2016
Shiga toxin-producing Escherichia coli (STEC) strains also called verotoxin-producing E. coli (VTEC) represent one of the most important groups of food-borne pathogens that can cause several human diseases such as hemorrhagic colitis (HC) and hemolytic – uremic syndrome (HUS) worldwide. The ability of STEC strains to cause disease is associated with the presence of wide range of identified and putativevirulence factors including those encoding Shiga toxin. In this study, we examined the
Aleksandra Januszkiewicz,
Waldemar Rastawicki
Polish Journal of Microbiology, Volume 65 , ISSUE 3, 261–269
Article | 24-March-2021
pasożytami. Panujące w piaskownicach warunki: podwyższona wilgotność oraz temperatura pomiędzy 22–30°C, sprzyjają wzrostowi bakterii i grzybów w tym dermatofitów. Wśród tych mikroorganizmów mogą być patogeny wywołujące grzybice, które nie tylko zmieniają powierzchnię skóry wywołując dyskomfort ale również są trudne w leczeniu. Coraz częściej w miejscach użyteczności publicznej pojawiają się mikroorganizmy, których obecność wydaje się być niepokojąca. Przykładem są bakterie Escherichia coli, których
Edyta Mazur,
Maria Jolanta Chmiel
Postępy Mikrobiologii - Advancements of Microbiology, Volume 60 , ISSUE 1, 77–89
short-communication | 18-February-2020
AFNAN A. AL-MNASER,
MARTIN J. WOODWARD
Polish Journal of Microbiology, Volume 69 , ISSUE 1, 121–125
research-article | 30-November-2018
, and parasitic protists (protozoa) and worms react in a different way to the disinfection process. Classical disinfection of sewage and by-products that arise during purification processes can be carried out by physical and chemical methods [10]. One of these methods is ultrasonic disintegration.
3.
METHODOLOGY OF LABORATORY TESTS
The aim of the experiments was to determine the effect of low-frequency ultra-sound (20 and 40 kHz) on the survival of Escherichia coli bacteria present in ultrapure
Eliza HAWRYLIK
Architecture, Civil Engineering, Environment, Volume 12 , ISSUE 3, 131–136
Research Article | 15-February-2020
This paper reports on the capabilities of a novel electromagnetic wave sensing method to detect and identify the presence of various pathogenic bacteria in aqueous media. In particular, the change in the electromagnetic wave signal in microwave frequency range is used as an indicator of bacteria presence. The assessment was conducted by recording reflected signal spectra when the sensor was in contact with deionised water, Escherichia coli, sterile nutrient broth and Pseudomonas aeruginosa
I. Nakouti,
O. Korostynska,
A. Mason,
A. I. Al-Shamma’a
International Journal on Smart Sensing and Intelligent Systems, Volume 7 , ISSUE 5, 1–6
Article | 01-March-2015
, designed for the identification and quantification of Escherichia coli O157:H7 bacteria covered by superparamagnetic beads, Dynabeads® MAX E.coli O157. The sensor characteristics, measurement system setup and the properties of the magnetic marker solution are discussed in detail.
Hamidreza Shirzadfar,
Mustapha Nadi,
Djilali Kourtiche,
Sotoshi Yamada,
Payman Shahabi
International Journal on Smart Sensing and Intelligent Systems, Volume 8 , ISSUE 1, 220–234
Mini Review | 30-June-2018
observed. It has also been revealed that depending on the concentration, salicylate induces expression of multi-drug resistance efflux pumps in Escherichia coli, Salmonella enterica subsp. enterica serotype Typhimurium, and Burkholderia cenocepacia. However, salicylate does not affect the expression of the resistance-nodulation-division efflux systems in Stenotrophomonas maltophilia and Acinetobacter baumannii. Most importantly, there were no effects of medicinal products containing some non-antibiotic
AGNIESZKA EWA LAUDY
Polish Journal of Microbiology, Volume 67 , ISSUE 2, 129–135
Original Paper | 10-December-2018
Abstract
A retrospective study was conducted among 498 patients with urinary tract infections (UTI) referred to our department from January 2013 to December 2015. This study was performed to evaluate the etiology of UTI and the antibiotic susceptibility profile of Escherichia coli (E. coli) as the main etiological factor in different age groups. Urine samples were examined using standard microbiological methods. Three hundred sixty-three samples (72.9%) were identified as E. coli, of which 29
MIKOŁAJ MICHNO,
ANTONI SYDOR,
MARTA WAŁASZEK,
WŁADYSŁAW SUŁOWICZ
Polish Journal of Microbiology, Volume 67 , ISSUE 4, 517–524
Original Paper | 04-September-2018
Human nerve growth factor β (β-NGF) is considered a major therapeutic agent for treatment of neurodegenerative diseases. We have previously reported the optimized conditions for β-NGF overproduction in Escherichia coli in a shake-flask culture. In this study the optimal %DO (dissolved oxygen) and post induction temperature values for improved production of β-NGF were found in the bioreactor scale using response surface methodology (RSM) as the most common statistical method. Also, for further
ZAHRA HAJIHASSAN,
POURIA GHOLAMI TILKO,
SEYEDEH MAHDIEH SADAT
Polish Journal of Microbiology, Volume 67 , ISSUE 3, 355–363
Article | 23-March-2020
1.
Wstęp
Pozajelitowe patogenne szczepy Escherichia coli ExPEC (extraintestinal patogenic E. coli) charakteryzują się różnorodnymi genotypami, jak i fenotypami, a w ich obrębie wyróżnia się, m.in. grupę uropatogennych szczepów Escherichia coli (uropathogenic E. coli, UPEC) odpowiedzialnych za 75–95% przypadków zakażeń układu moczowego (ZUM), zarówno u dzieci, jak i osób dorosłych [4, 6, 10, 33, 34]. UPEC mogą kolonizować nabłonek układu moczowego człowieka, lecz o ich chorobotwórczości i
Sylwia Joanna Chmielewska,
Katarzyna Leszczyńska
Postępy Mikrobiologii - Advancements of Microbiology, Volume 59 , ISSUE 1, 25–37
Original Paper | 28-June-2017
surrounding the armA gene. Out of 123 isolates, 66 (53.66%) were resistant to at least oneaminoglycoside antibiotic. Only one Escherichia coli isolate (E9ECMO) which was totally resistant to all tested aminoglycosides, was confirmed to have the armA gene in association with blaTEM-1, blaCTX-M-15, blaCTX-M-14 and aac(6)-Ib genes. The armA gene was found to be carried on a large A/C plasmid. Genetic mapping of the armA surrounding region revealed, for the first time, the association of armA with aac(6)-Ib
Mona T. Kashef,
Omneya M. Helmy
Polish Journal of Microbiology, Volume 66 , ISSUE 2, 163–169
Article | 30-November-2018
1.
Introduction
Escherichia coli is a facultative anaerobe that is part of the human colon’s microbiota. This organism colonizes the intestines of newborns as early as in the first hours of their life. Most E. coli rods are harmless and its presence is limited to the digestive tract. Nevertheless, in weakened people, patients suffering from immunosuppression and as a result of damage to the intestinal epithelial layer, even these non-pathogenic strains of E. coli may cause extraintestinal
Michał Turniak,
Beata Sobieszczańska
Postępy Mikrobiologii - Advancements of Microbiology, Volume 58 , ISSUE 2, 143–152
Article | 04-January-2021
(mikrobiologicznych, biochemicznych, biofizycznych, bioinformatycznych czy proteomicznych) zaowocowało w ostatnich latach szczegółową charakterystyką funkcjonowania sieci Dsb modelowej bakterii Escherichia coli (EcDsb). Dane te przedstawia wiele znakomitych publikacji przeglądowych. Ostatnio opublikowane to między innymi [3, 40, 43]. Doskonalenie metod sekwencjonowania materiału genetycznego ujawniło różnorodność systemów Dsb w świecie mikroorganizmów. Różnice dotyczą liczby białek Dsb konkretnej kate gorii, ich
Anna Marta Banaś,
Anna Petrykowska,
Elżbieta Katarzyna Jagusztyn-Krynicka
Postępy Mikrobiologii - Advancements of Microbiology, Volume 59 , ISSUE 4, 345–355
Article | 05-October-2019
dokładnie dla modelowych bakterii, Gram-ujemnych i Gram-dodatnich. U Escherichia coli poziom cztero- i pięciofosforanu guanozyny kontrolowany jest przez aktywność produktów genów relA i spoT. ppGpp i pppGpp mogą być syntetyzowane przez białka RelA i SpoT, a powstają odpowiednio z GDP lub GTP oraz ATP. Z kolei dwufunkcyjne SpoT ma również zdolność hydrolizy ppGpp do GDP i pirofosforanu (PPi) oraz pppGpp do GTP i PPi. Większość Gammaproteobacteria i część Betaproteobacteria, takich jak E. coli, Salmonella
Klaudyna Krause,
Klaudia Milewska,
Agnieszka Szalewska-Pałasz
Postępy Mikrobiologii - Advancements of Microbiology, Volume 58 , ISSUE 3, 247–258
Short Communication | 30-March-2017
In this study, we describe a method for discriminating pathogenic bacteria with a dye. First, we determined that among several colours tested, the sunset yellow pigment easily coloured Escherichia coli bacteria yellow. Next, we demonstrated that E. coli O157:H7, Shigella flexneri O301, Staphylococcus aureus and Bacillus subtilis could all be well marked by sunset yellow pigment. Finally, we performed bacterial viability assays and found there was no effect on bacterial growth when in co-culture
Peng Gao,
Chengming Sun,
Yuxia Li,
Xiaohua Zou,
Xun Wu,
Yan Ling,
Caifu Luan,
Huipeng Chen
Polish Journal of Microbiology, Volume 66 , ISSUE 1, 113–117
short-communication | 05-December-2019
ASHVEEN BAINS,
JAMES W. WILSON
Polish Journal of Microbiology, Volume 68 , ISSUE 4, 559–563
short-communication | 07-June-2021
been many reports about plasmid resistance to colistin among strains isolated from human infections (Kluytmans 2017; Elbediwi et al. 2019). In Poland, the first Escherichia coli strain with the mcr-1 gene was described in 2016 (Izdebski et al. 2016). However, we do not have more information about the presence of mcr genes in Poland. As β-lactam antibiotics are “first-line” drugs in the treatment of infections caused by Enterobacterales, the susceptibility of strains to this group of antimicrobial
ELŻBIETA M. STEFANIUK,
ALEKSANDRA KOZIŃSKA,
IZABELA WAŚKO,
ANNA BARANIAK,
STEFAN TYSKI
Polish Journal of Microbiology, Volume 70 , ISSUE 2, 283–288
Short Communication | 04-December-2017
The authors studied the in vitro antibacterial activity of the photo-activated porphyrin meso-tri(N-methyl-pyridyl), mono(N-tetradecyl-pyridyl)porphine (C14) against four multidrug-resistant bacteria: Staphylococcus aureus, Enterococcus faecalis (Gram-positive), Escherichia coli, Pseudomonas aeruginosa (Gram-negative). Using 10 μg/ml of porphyrin and 60 sec irradiation we observed the remarkable susceptibility of S. aureus and E. faecalis to treatment while, under the same conditions, E
Moreno Bondi,
Anna Mazzini,
Simona De Niederhäusern,
Ramona Iseppi,
Patrizia Messi
Polish Journal of Microbiology, Volume 66 , ISSUE 4, 533–536
Original Paper | 04-December-2017
antimicrobial activity against the tested strains was demonstrated by black elder (Sambucus nigra L.), black locust (Robinia pseudoacacia L.) and lingonberry (Vaccinium vitis-idaea L.) extracts. The study showed the diverse morphological activity of specific parts of elderberry and quince, which is the effect of different polyphenolic profile of these plants. The yeast Saccharomyces cerevisiae, Escherichia coli, Pseudomonas putida and Bacillus subtilis showed the highest sensitivity to the effect of
Monika Cioch,
Paweł Satora,
Magdalena Skotniczy,
Dorota Semik-Szczurak,
Tomasz Tarko
Polish Journal of Microbiology, Volume 66 , ISSUE 4, 463–472
Research Article | 22-May-2019
Bacterial Dsb (disulfide bond) enzymes are involved in the oxidative folding of many proteins, through the formation of disulfide bonds between thiol groups of cysteine residues. This process is critical for the correct folding and structural stability of many secreted and membrane proteins. The rapidly expanding number of sequenced bacterial genomes has revealed the enormous diversity among bacterial Dsb systems. While the Escherichia coli oxidative protein folding has been studied in great
Elżbieta Katarzyna Jagusztyn-Krynicka,
Anna Marta Banaś,
Magdalena Joanna Grzeszczuk
Postępy Mikrobiologii - Advancements of Microbiology, Volume 56 , ISSUE 3, 326–334
Review | 21-April-2020
the critical C3 convertase step. By this mechanism,DAF acts to protect autologous cells and tissues from complement-mediated damage and hence can play a role in preventing or modulating autoimmune disease and inflammation. The use of recombinant DAF as a therapeutic agent in autoimmunity and inflammation, and of DAF transgenic animals in xenotransplantation, is being actively investigated. Additionally, DAF serves as a receptor for certain strains of Escherichia coli and certain types of
Douglas M. Lublin
Immunohematology, Volume 21 , ISSUE 2, 39–47
research-article | 30-November-2018
extracted from the overnight culture using a QIAprep Spin Miniprep Kit (Qiagen) and the resulting plasmids were sequenced for verification at the University of Illinois Roy J. Carver Biotechnology Center.
Complementation of ΔbioB Escherichia coli with avirulent and virulent alleles of HgBioB
A kanamycin-resistant E. coli vitamin B7 auxotroph (K-12 Keio Collection: JW0758-1) was obtained from the Coli Genetic Stock Center at Yale University and used to prepare electrocompetent cells as described by
Khee Man Kwon,
Sadia Bekal,
Leslie L. Domier,
Kris N. Lambert
Journal of Nematology, Volume 51 , 1–12
original-paper | 05-December-2019
do not exhibit toxicity for mammalian cells (Milic et al. 2015; Zhang et al. 2016a). However, some cytostatic and anti-cancer activity is reported at these concentrations (AshaRani et al. 2009). Antibacterial activity of AgNPs is rather well documented in the literature, mainly against the following species: Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli (Yoon et al. 2007; Li et al. 2010; Guzman et al. 2012, Kanawaria et al. 2018). Some information
BARBARA ŻAROWSKA,
TOMASZ KOŹLECKI,
MICHAŁ PIEGZA,
KATARZYNA JAROS-KOŹLECKA,
MAŁGORZATA ROBAK
Polish Journal of Microbiology, Volume 68 , ISSUE 4, 515–525
Original Paper | 28-June-2017
The caecal chyme of pigs was incubated anaerobically in McDougall buffer with and without fumonisin B1 (5 μg/ml) for 0, 24 and 48 h. The plate count agar technique was applied for enumerating the amount of bacteria including aerobic, anaerobic bacteria, coliform, Escherichia coli and Lactobacillus sp. The quantitative polymerase chain reaction was also performed to estimate the number of copies of the total bacteria, Lactobacillus, Bacteroides and Prevotella. No significant differences in
Huu Anh Dang,
Attila Zsolnai,
Melinda Kovacs,
István Bors,
András Bónai,
Brigitta Bóta,
Judit Szabó-Fodor
Polish Journal of Microbiology, Volume 66 , ISSUE 2, 245–250
Original Research | 04-September-2018
Staphylococcus aureus and Escherichia coli. These results together suggest that supplementation of both proteases and probiotics in SSF improves the nutritional value of fermented soybean meal and this is suitable as a protein source in animal feed.
LI-WEN SU,
YEONG-HSIANG CHENG,
FELIX SHIH-HSIANG HSIAO,
JIN-CHENG HAN,
YU-HSIANG YU
Polish Journal of Microbiology, Volume 67 , ISSUE 3, 297–305
original-paper | 28-June-2019
a more complete picture of bacterial infections and to help clinicians and decision-making departments undertake the proper decisions for patients and antibiotic use.
Experimental
Materials and Methods
Based on the data from a general hospital in Zhengzhou, Henan province, China from 2011 to 2016, five dominant bacteria (Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Acinetobacter baumannii) were investigated in this study. The antibiotic
TINGTING MAO,
HUIJUAN ZHAI,
GUANGCAI DUAN,
HAIYAN YANG
Polish Journal of Microbiology, Volume 68 , ISSUE 2, 225–232
original-paper | 23-November-2020
allow for a numerical evaluation of the ability of bacteria to form biofilms. In this study, the quantitative assays were adopted based on its accuracy, reliability, and potential to enable precise quantification instruments.
Biofilm forming pathogens (Salmonella Typhimurium, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis) have been isolated in food and food processing plants in developed and developing countries (Dourou et al. 2011; Cook et al. 2012
STEPHEN ABIOLA AKINOLA,
MPINDA EDOAURD TSHIMPAMBA,
MULUNDA MWANZA,
COLLINS NJIE ATEBA
Polish Journal of Microbiology, Volume 69 , ISSUE 4, 427–439
Research Article | 24-May-2019
phenomenon. A contact-dependent growth inhibition (CDI), primarily found in Escherichia coli strains, utilizes CdiB/CdiA protein of type Vb secretion system, described also as two-partner secretion (TPS) system, to inhibit growth of non-kin strains, where cell contact is required. Presence of an intracellular small immunity protein (CdiI) protects E. coli cells from autoinhibition. Other bacterial competition system, involved mainly in the nodulation process of Rhizobium leguminosarum bv. Trifolii strain
Dawid Gmiter,
Grzegorz Czerwonka,
Wiesław Kaca
Postępy Mikrobiologii - Advancements of Microbiology, Volume 57 , ISSUE 4, 360–373
Article | 30-November-2018
%). Moreover, 55–64% of the isolates demonstrated cross-resistance to ciprofloxacin and nalidixic acid.
Table II
Percentage of commensal Escherichia coli strains resistant to selected antimicrobials, isolated from fattening pigs
Country/average in EU
Number of strains tested
Percentage of resistant strains
Gentamicin
Chloramphenicol
Ampicillin
Cefotaxime
Ceftazidime
Ciprofloxacin
Colistine
Sulfamethoxazole
Tetracicline
Spain
170
4.7
35.3
77.1
2.9
2.9
44.7
0.6
63.5
88.8
Portugal
142
Marian Binek,
Magdalena Kizerwetter-Świda,
Magdalena Rzewuska,
Dorota Chrobak-Chmiel,
Agnieszka Sałamaszyńska-Guz
Postępy Mikrobiologii - Advancements of Microbiology, Volume 58 , ISSUE 3, 259–270