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  • Journal Of Nematology


research-article | 30-November-2018

In vivo production of entomopathogenic nematodes using Galleria mellonella: costs and effect of diets on nematode pathogenicity

trials) according to Shapiro-Ilan et al. (2002). In vivo nematode production yields nematodes with good virulence potential (Shapiro-Ilan et al., 2000). Under field conditions, application of EPN in insect host cadavers can reduce the quantity of nematodes required for control per unit area compared with their application in water or other solvents (Shapiro-Ilan et al., 2012). The typical host used to mass-produced EPN is the greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae) (Testa and

Régina Kotchofa, Hugues Baimey

journal of nematology, Volume 51 , 1–15

Research Article | 03-December-2018

Enhanced entomopathogenic nematode yield and fitness via addition of pulverized insect powder to solid media

(Galleria mellonella, Tenebrio molitor, and Lucillia sericata) at three dose levels (1, 3, and 5%). The impact of insect powder was assessed on infective juvenile (IJ) yield in solid media. Additionally, IJs produced in solid culture were subsequently assessed for virulence, and progeny production in a target insect, Spodoptera litura. The dose level of larval powder had a significant effect on IJ yield in both trials, whereas insect type had significant effect on IJ yield in trial 1 but not in trial 2

Shiyu Zhen, Yang Li, Yanli Hou, Xinghui Gu, Limeng Zhang, Weibin Ruan, David Shapiro-Ilan

Journal of Nematology, Volume 50 , ISSUE 4, 495–506

Research Article | 17-October-2018

Survival and Infectivity of Entomopathogenic Nematodes Formulated in Sodium Alginate Beads

Galleria mellonella and the infective juveniles emerged were subjected to two capture treatments: white traps and plaster of Paris, the latter was utilized as a pre-conditioning treatment. A total of 1,000 infective juveniles were formulated in each sodium alginate bead with or without an alginate coating. The beads were stored at 23 ± 3°C and a bidistilled water suspension of nematodes was set as a control. The survivorship of these formulates and their infectivity on Galleria mellonella larvae were

Jaime Ruiz-Vega, Carlos I. Cortés-Martínez, Cipriano García-Gutiérrez

Journal of Nematology, Volume 50 , ISSUE 3, 273–280

research-article | 30-November-2018

Conspecific pheromone extracts enhance entomopathogenic infectivity

insects: the greater wax moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae) and the citrus root weevil, Diaprepes abbreviatus (L.) (Curculionidae: Coleoptera). G. mellonella is a highly susceptible model host that is used as a model insect used in routine laboratory assays or commercial production of EPNs (Shapiro-Ilan et al., 2012). D. abbreviatus is a major pest of citrus that has been targeted extensively with EPNs on a commercial level (McCoy et al., 2007). Materials and methods EPNs and

David I. Shapiro-Ilan, Fatma Kaplan, Camila Oliveira-Hofman, Paul Schliekelman, Hans T. Alborn, Edwin E. Lewis

Journal of Nematology, Volume 51 , 1–5

research-article | 30-November-2019

Isolation, identification, and pathogenicity of Steinernema carpocapsae and its bacterial symbiont in Cauca-Colombia

equipment (Poinar, 1972; Hazir et al., 2003; Van Zyl and Malan, 2014), and can be propagated in mass either in vivo using host insects such as Galleria mellonella (Lepidoptera: Pyralidae) or in vitro in bioreactors using artificial culture media (Shapiro-Ilan et al., 2014). EPNs life cycle has six states: egg, four juvenile stages, and adults: males, females, or hermaphrodites (Shapiro-Ilan et al., 2014). EPNs of the Steinernematidae family are characterized by symbiotic associations with bacteria of

Esteban Neira-Monsalve, Natalia Carolina Wilches-Ramírez, Wilson Terán, María del Pilar Márquez, Ana Teresa Mosquera-Espinosa, Adriana Sáenz-Aponte

Journal of Nematology, Volume 52 , 1–16

Article | 21-July-2017

Oscheius microvilli n. sp. (Nematoda: Rhabditidae): A Facultatively Pathogenic Nematode from Chongming Island, China

features of the bursa, and molecular data. The new species is facultatively associated with a bacterial strain of Serratia. The LC50 of this novel nematode against Galleria mellonella was 69.1 dauer juveniles per milliliter after 48 hr of infection.


Journal of Nematology, Volume 49 , ISSUE 1, 33–41

Research Article | 03-December-2018

Biological characterization of the entomopathogenic nematode, Steinernema innovationi: a South African isolate

Entomopathogenic nematode species perform differently under different environmental conditions; therefore, the authors investigated the biological and environmental characteristics that could optimize performance of Steinernema innovationi. The authors studied the effect of temperature on infectivity and reproduction, the foraging behavior and host range. Thermal activity was optimal between 22 and 25°C. Highest infective juvenile (IJ) yields in last instar Galleria mellonella were observed at

Tshimangadzo Ramakuwela, Justin Hatting, Mark D. Laing, Nicolene Thiebaut, Selcuk Hazir

Journal of Nematology, Volume 50 , ISSUE 4, 1–10

research-article | 19-March-2020

Natural occurrence and distribution of entomopathogenic nematodes (Steinernematidae, Heterorhabditidae) in Viti Levu, Fiji Islands

verge. To our knowledge, no EPN based products were ever released at any of the 478 sites sampled. Site locations were plotted on base map using software ArcMap version 10.4.1. The soil sampler was sterilized with 70% ethanol before leaving the sampling site. Isolation of entomopathogenic nematodes The soil samples were processed on the site to isolate native EPNs using wax moth Galleria mellonella (Lepidoptera: Pyralidae) larvae as the insect bait (Bedding and Akhurst, 1975). Freshly harvested G

Sumeet Kour, Uma Khurma, Gilianne Brodie, Selcuk Hazir

journal of nematology, Volume 52 , 1–17

Research Article | 31-May-2018

Incidence of Oscheius onirici (Nematoda: Rhabditidae), a potentially entomopathogenic nematode from the marshlands of Wisconsin, USA

. Nonetheless, this nematode is capable of infecting and killing the sparganothis fruitworm Sparganothis sulfureana Clemens (Lepidoptera: Tortricidae), the brown-banded cockroach Supella longipalpa Fabricius (Blattodea: Ectobiidae), and the cranberry fruitworm Acrobasis vaccinii Riley (Lepidoptera: Pyralidae), under laboratory conditions, and each in less than 72 hr. The mealworm Tenebrio molitor Linnaeus (Coleoptera: Tenebrionidae) and the greater wax moth Galleria mellonella Linnaeus (Lepidoptera

Weimin Ye, Shane Foye, Ann E. MacGuidwin, Shawn Steffan

Journal of Nematology, Volume 50 , ISSUE 1, 9–26

research-article | 30-November-2020

Virulence of Steinernema ceratophorum against different pest insects and their potential for in vivo and in vitro culture

clone the ITS1-5.8S-ITS2 rRNA gene, and D2-D3 expansion segment of the 28 S rRNA gene, respectively (Nguyen et al., 2006). The nematode was identified as Steinernema ceratophorum Jian, Reid and Hunt. IJ of S. ceratophorum were reared in last instar larvae of the greater wax moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae), and harvested by using White traps (Kaya and Stock, 1997). IJ suspension was concentrated to 2,000 IJ/ml, stored at 15°C, 100 rpm shaker and used within 2 weeks. Bioassays

Xun Yan, Guimei Chen, Yuqing Chen, Bingjiao Sun, Xinghui Gu, Weibing Ruan, Richou Han

Journal of Nematology, Volume 53 , 1–12

research-article | 18-March-2020

Soil texture, infective juvenile concentration, and soil organic matter influence the efficacy of Steinernema feltiae isolate Lican Ray

of soil OM on soil inhabitants in general (Hoitink and Boehm, 1999; Davey et al., 2019), few have focused on EPN performance (Kapranas et al., 2017). For instance, Herren et al. (2018) observed higher survival and virulence of S. feltiae on larvae of Galleria mellonella (Lepidoptera: Pyralidae) in soils with added mature compost. Some authors propose that the compost could be used as a carrier for EPN application in the field (Herren et al., 2018; Georgis et al., 2006) as it could protect them

Gabriela Lankin, Giselle Vidal-Retes, Geraldine Allende, Carlos Castaneda-Alvarez, Ernesto San-Blas, Erwin Aballay

Journal of Nematology, Volume 52 , 1–11

research-article | 30-November-2019

Oscheius myriophila (Nematoda: Rhabditida) isolated in sugar cane soils in Mexico with potential to be used as entomopathogenic nematode

described by Orozco et al. (2014). Each soil sample was baited by placing 12 fifth-instar Galleria mellonella Linnaeus (Lepidoptera: Pyralidae) wax worms in speech boxes containing sampled soil, and they were stored at room temperature in the dark for a week. Subsequently, the boxes were checked daily until the wax worms began to show signs of infection, at which time the insects were removed from the boxes, rinsed with water and stored in modified white traps (Orozco et al., 2014) until the EPNs

Iveth del Rocio Castro-Ortega, Juan Manuel Caspeta-Mandujano, Ramón Suárez-Rodríguez, Guadalupe Peña-Chora, José Augusto Ramírez-Trujillo, Karina Cruz-Pérez, Iván Arenas Sosa, Víctor Manuel Hernández–Velázquez

Journal of Nematology, Volume 52 , 1–8

research-article | 30-November-2018

Touch-stimulation increases host-seeking behavior in Steinernema Carpocapsae

, 1997). Last-instar Galleria mellonella were placed in a 6 cm petri dish and infected with approximately 30 nematodes per host. They were then incubated at room temperature (approximately 23°C) for 7 to 10 d. After this incubation period, infected and deceased hosts were placed on white traps, which were incubated at room temperature for another 7 to 10 d. IJs were collected from the white traps, rinsed three times with tap water, and placed within cell culture flasks. A portion of these IJs were

Tiffany Baiocchi, Lauren Braun, Adler R. Dillman

journal of nematology, Volume 51 , 1–5

research-article | 30-November-2019

Activity of Steinernema colombiense in plant-based oils

were: (i) to evaluate the survival and virulence of S. colombiense against last instar larvae of Galleria mellonella (Lepidoptera: Pyralidae) when combined with coconut and olive oils as model adjuvants at different temperatures and times, and (ii) to evaluate the efficacy of using these plant-based oil combined with S. colombiense and B. bassiana. Material and methods Organisms, oils, and substrates We conducted the experiments by employing S. colombiense isolated from Mexico and donated by

Gabriela Castruita-Esparza, Francisco Ángel Bueno-Pallero, Rubén Blanco-Pérez, Lídia Dionísio, Teodulfo Aquino-Bolaños, Raquel Campos-Herrera

Journal of Nematology, Volume 52 , 1–12

research-article | 30-November-2020

Ecological Characterisation of Native Isolates of Heterorhabditis indica from Viti Levu, Fiji Islands

Sumeet Kour, Uma Khurma, Gilianne Brodie

Journal of Nematology, Volume 53 , 1–20

research-article | 30-November-2020

The impact of chemical nematicides on entomopathogenic nematode survival and infectivity

0.6 Succinate dehydrogenase inhibitor SC Fosthiazate 150 Versus® 150EC Hektas©, Turkey 10 Organophosphate EC Metam potassium 510 Rotabent® Hektas©, Turkey 1000 Dithiocarbamate Salt SL Fenamiphos 400 Nemacur® 400EC Hektas©, Turkey 75 Organophosphate EC Notes: Formulation; EC: emulsifiable concentrate; SC: suspension concentrate; SL: Soluble liquid. Maintenance of galleria mellonella, EPNs, and PPNs Galleria mellonella (Lepidoptera: Pyralidae), the greater wax moth, was used in

Mustapha Touray, Harun Cimen, Sebnem H. Gulsen, Derya Ulug, Dolunay Erdogus, David Shapiro-Ilan, Selcuk Hazir

Journal of Nematology, Volume 53 , 1–17

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