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research-article | 30-November-2020

Genetic variation within a species of parasitic nematode, Skrjabingylus chitwoodorum, in skunks

Skrjabingylus. The objective of this study was to use COI sequences to determine the level of genetic variation within S. chitwoodorum, to describe the patterns of variation with respect to geographical sampling locality, and to determine the phylogenetic relationship of S. chitwoodorum to other Skrjabingylus species. Material and methods A total of 19 Skrjabingylus samples that were reported in Hughes et al. (2018) were used for the present study. Only samples that were preserved in ethanol or were frozen

Allie N. Denham, Malorri R. Hughes, Robert C. Dowler, Nicholas J. Negovetich, Loren K. Ammerman

Journal of Nematology, Volume 53 , 1–8

Article | 15-February-2021

An update on the Scianna blood group system

genetic variation seen in ERMAP. Mutations that interfere with such vital functions would likely be deleterious. There continues to be interest and enthusiasm for continuing work on the Scianna system antibodies and their underlying diversity, revealed through continued patient case reports and cutting-edge approaches like “big data” genetics and recombinant protein serology.

P.A.R. Brunker, W.A. Flegel

Immunohematology, Volume 35 , ISSUE 2, 48–50

research-article | 30-November-2020

Phylogenetics and genetic variation of Heligmosomoides thomomyos in Western pocket gophers (Thomomys spp.)

is the result of a negative value for within sample population structure, which is almost certainly driven by low or uneven sample sizes among populations due to sampling limitations. Consequently, it should be interpreted as a statistical artifact indicative of unevenly distributed genetic variation among the sampled populations (Meirmans, 2007). Finally, we also found a modest positive association between geographic and genetic distances for the COI gene (Mantel test, r = 0.472, p = 0.0198

Malorri R. Hughes, Alexandra A. Gibson, Emily R. Wolfe, Cecily D. Bronson, Deborah A. Duffield

Journal of Nematology, Volume 53 , 1–11

research-article | 17-March-2020

Molecular approach to confirm traditional identification of Radopholus similis sampled in Tanzania

Doreen M. Mgonja, Gladness E. Temu, Joseph C. Ndunguru, Magreth F. Mziray, Sylvester L. Lyantagaye, Nessie D. Luambano

Journal of Nematology, Volume 52 , 1–8

Article | 26-October-2019

Multiplex ligation-dependent probe amplification assay for blood group genotyping, copy number quantification, and analysis of  RH variants

the MLPA to facilitate these different types of genetic variation is a prerequisite in blood group typing. An MLPA assay allows the simultaneous detection of up to 50 polymorphisms in a single tube. The blood group MLPA currently consists of three separate probe pools targeting 104 different blood group alleles of 18 blood group systems. The assay is performed in a 96-well plate; therefore, a maximum of 32 genomic DNA samples can be processed simultaneously. Results are available within 24 hours

Barbera Veldhuisen, C. Ellen van der Schoot, Masja de Haas

Immunohematology, Volume 31 , ISSUE 2, 58–61

Article | 17-February-2021

May the FORS be with you: a system sequel

Project.8 These data were used, and genetic variation at the GBGT1 locus was studied by Hult et al.9 The two most common alleles (GBGT1*01N.01, GBGT1*01N.02) constitute 89 percent of all alleles and, of the 66 remaining alleles reported in Erythrogene, there were six different alleles containing c.363C>A (rs35898523), of which GBGT1*02N (allele frequency 3.6%) was the most common. This single nucleotide polymorphism (SNP) gives rise to a severely truncated protein predicted to lack the whole

A.K. Hult, M.L. Olsson

Immunohematology, Volume 36 , ISSUE 1, 14–18

Research Article | 20-May-2019

FRANCISELLA TULARENSIS – REVIEW 

variable intergenic regions may be also applicable to detection, differentiation and determination of genetic variation among F. tularensis strains. In addition, the above methods could be successfully used in molecular characterization of tularaemia strains from humans and animals isolated in screening research, and during epizootic and epidemic outbreaks.

Piotr Cieślik, Józef Knap, Agata Bielawska-Drózd

Postępy Mikrobiologii - Advancements of Microbiology, Volume 57 , ISSUE 1, 58–67

Article | 30-November-2018

PARECHOVIRUSES – UNDERESTIMATED RISK

Arleta Krzysztoszek, Magdalena Wieczorek

Postępy Mikrobiologii - Advancements of Microbiology, Volume 58 , ISSUE 3, 301–315

Report | 16-October-2019

Mixed-field agglutination observed in column agglutination testing is not always associated with the A3 subgroup

antibody was performed, MFA was not observed in 9 of 11 samples with previously observed MFA from routine CAT, which were then interpreted as A2. From PCR-SBT performed in only exon 7 of the ABO gene, 7 of 13 sample results were consistent with ABO*A2 or ABO*AW alleles. Two samples suspected to be A2 or A3 had an ABO*AW allele. In two samples suspected to be Aweak, no mutation was detected in ABO exon 7, suggesting genetic variation elsewhere in the gene. Although other coding exons were not examined

Nampeung Anukul, Nipapan Leetrakool, Praijit Tanan, Poonsub Palacajornsuk, Phennapha Klangsinsirikul

Immunohematology, Volume 34 , ISSUE 2, 49–56

research-article | 30-November-2020

Characterization of Globodera ellingtonae Populations from Chile Utilizing Whole Genome Sequencing

SocaireB cysts. The relationship among the primary G. ellingtonae Talabre, Socaire, and INRAE Antofagasta clades remained unresolved despite the large number of loci used in the phylogeny. Cysts from the G. ellingtonae Oregon population subtended the Chilean clades and placed on a long branch representing approximately twice the genetic variation observed among all Chilean populations. Figure 2: Nuclear single nucleotide polymorphism (SNP) phylogenetic reconstruction of 23 Globodera ellingtonae cysts

C.N. Hesse, I. Moreno, O. Acevedo Pardo, H. Pacheco Fuentes, E. Grenier, L. M. Dandurand, I. A. Zasada

Journal of Nematology, Volume 53 , 1–9

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