research-article | 29-March-2019
their interaction with the insect-pest.
Hence, in an effort to increase the survival time and infectivity of EPNs on ticks, this study aimed to evaluate: (i) the effect of five vegetable oil emulsions on the survival and infectivity of S. carpocapsae, S. websteri and H. bacteriophora on ticks in laboratory and (ii) the control effectiveness (CE) of application of EPNs in oil emulsion on ticks present in infested dogs (Canis lupus familiaris) in field conditions.
Materials and methods
Description
Teodulfo Aquino-Bolaños,
Jaime Ruiz-Vega,
Yolanda D. Ortiz Hernández,
Julio C. Jiménez Castañeda
Journal of Nematology, Volume 51 , 1–10
research-article | 30-November-2020
) that describes three clades within the genus Heterorhabditis (Fig. 1). We chose at least one species from each Heterorhabditis clade, two from the clade containing H. bacteriophora, and a single Steinernema species (S. feltiae). We assessed the responsiveness of five species to their own macerate, and then tested the responses of all five species to H. bacteriophora-derived macerate in a common experiment. We hypothesized that responsiveness to host cues would be conserved, while species-specific
Hilal Erdogan,
Glen Stevens,
Asa Stevens,
David Shapiro-Ilan,
Fatma Kaplan,
Hans Alborn,
Edwin Lewis
Journal of Nematology, Volume 53 , 1–9
research-article | 30-November-2020
D. balteata, the infectivity varied for the sequestering D. virgifera virgifera. Also, they detected some intraspecific variability in the impact of the benzoxazinoids on the infectivity of H. bacteriophora populations, although most of them were resistant. Finally, a recent study by Levy et al. (2020) revealed intraspecific variability on the tolerance to heat and desiccation. By using several bioassays, Levy et al. (2020) defined which populations performed better under stressing conditions
Raquel Campos-Herrera,
Ignacio Vicente-Díez,
Magda Galeano,
Maryam Chelkha,
María del Mar González-Trujillo,
Miguel Puelles,
David Labarga,
Alicia Pou,
Javier Calvo,
José Eduardo Belda
Journal of Nematology, Volume 53 , 1–14
research-article | 30-November-2020
EPN species like H. bacteriophora and S. carpocapsae (Browning et al., 2004; Glazer et al., 1997; Hara and Kaya, 1982; Rovesti et al., 1988, Rovesti and Deseo, 1990). These studies mostly aimed to assess the compatibility of these compounds with EPNs based on the survival and infectivity of IJs. There, however, is a need to expand on the diversity of nematicides that are tested for impact on EPN, and their effects on the penetration efficacy and reproductive capacity; chemotaxis and longer-term
Mustapha Touray,
Harun Cimen,
Sebnem H. Gulsen,
Derya Ulug,
Dolunay Erdogus,
David Shapiro-Ilan,
Selcuk Hazir
Journal of Nematology, Volume 53 , 1–17
research-article | 30-November-2020
move after probing using a needle, the nymphs were regarded to be dead.
In the second virulence experiment, four EPN species [H. bacteriophora (VS strain), H. floridensis (K22 strain), S. carpocapsae (All strain), and S. rarum (17C&E)] with high virulence to MEAM1 nymphs were selected based on the outcome of the first virulence experiment above. The virulence to MEAM1 third instar nymphs was assessed on snap bean and tomato leaves. The first evaluation was conducted three times (3 trials over time
Yinping Li,
George N. Mbata,
David I. Shapiro-Ilan
Journal of Nematology, Volume 53 , 1–8
research-article | 30-November-2018
. enterolobii to tomato in the presence of Steinernema brazilense PONTO2C, S. feltiae (Filipjev) IBCB47, S. rarum (Doucet) PAM25 (Rhabditida: Steinernematidae), Heterorhabditis amazonensis IBCB10 and H. bacteriophora (Poinar) HB (Rhabditida: Heterorhabditidae) in vitro, in order to verify if the EPNs promote mortality or interfere in the hatching of juveniles of M. enterolobii.
Material and methods
The experiments were carried out in incubator chambers (biochemical oxygen demand—BOD) at 25°C, 70% of
Alixelhe Pacheco Damascena,
Júlio César Antunes Ferreira,
Marylia Gabriella Silva Costa,
Luis Moreira de Araujo Junior,
Silvia Renata Siciliano Wilcken
journal of nematology, Volume 51 , 1–8
research-article | 30-November-2019
, KM016361.1, KM016345.1, JN098451.1, AB243439.1). Similarly, S. feltiae 2 species was found 100% identical to S. feltiae (Accessions: MK294325.1, MK294320.1, KM016352.1, KM016339.1, AF121050). Heterorhabditis bacteriphora was 100% conspecific to a number of H. bacteriophora isolates in NCBI (MK072810.1, MK421482.1, MG551676.1, KT378450.1, KT378448).
Discussion
The purpose of this survey was to see if EPNs were present in the Golden Triangle area of Montana, and if so, to explore the patterns of their
Ramandeep K. Sandhi,
Ratnasri Pothula,
Satyendra K. Pothula,
Byron J. Adams,
Gadi V.P. Reddy
Journal of Nematology, Volume 52 , 1–11
research-article | 30-November-2019
cups and placing them in the incubator, we observed the cups daily for adult emergence until 30 days post treatment application. Adults that successfully emerged were considered to have survived the nematode treatment. All other experimental parameters were the same as described above except we used 10 pupae per replicate (based on the availability of insects).
Pot bioassay
Five nematode species, S. riobrave, S. carpocapsae, S. feltiae, H. indica, and H. bacteriophora were selected for the pot
Muhammad Usman,
Sehrish Gulzar,
Waqas Wakil,
Jaime C. Piñero,
Tracy C. Leskey,
Laura J. Nixon,
Camila Oliveira-Hofman,
Shaohui Wu,
David Shapiro-Ilan
Journal of Nematology, Volume 52 , 1–9
Article | 21-July-2017
, H. bacteriophora demonstrated low virulence on the pest larvae in petri tests with a LC50 = 426.92 IJ/larva. Hence, both Steinernema species were selected for subsequent experiments. The penetration rate for S. feltiae and S. carpocapsae into the hemocoel of the pest was 43% and 31%, respectively, and the corresponding reproduction rate was 15,452 IJ/larva for S. feltiae and 18,456 IJ/larva for S. carpocapsae. The gathered data from those in vitro tests were used for a field assay
ZAHRA MEMARI,
JAVAD KARIMI,
SHOKOOFEH KAMALI,
SEYED HOSSEIN GOLDANSAZ,
MOJTABA HOSSEINI
Journal of Nematology, Volume 48 , ISSUE 4, 261–267