original-paper | 08-September-2020
°40′16.68″). Three sampling locations, approximately 1000 m apart, were selected, and four single healthy plant samples, were randomly gathered from each sampling location in September 2018. Samples collected included leaves, stems, and roots. All the plant samples were placed into the aseptic sample box immediately and stored at –80°C. The plant tissues were surface-sterilized following the previously described method (Correa-Galeote et al. 2014).
DNA isolation, PCR amplification, and Illumina
Polish Journal of Microbiology, Volume 69 , ISSUE 3, 283–291