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  • Immunohematology

 

Review | 09-November-2020

Review: conditions causing weak expression of Kell system antigens

Ragnhild Øyen, Gregory R. Halverson, Marion E. Reid

Immunohematology, Volume 13 , ISSUE 3, 75–79

Article | 16-October-2019

Dithiothreitol treatment of red blood cells

been excluded in other tests. Quality Control When performing treatment to destroy RBC antigens, K+ RBCs should be DTT-treated with each test batch. The treated and untreated K+ RBCs are then tested with anti-K. The treated RBCs should be nonreactive; otherwise, the DTT treatment was not adequate. Other antigens of the Kell system can also serve as controls.1 If dispersal of spontaneous agglutination is being performed, test the treated RBCs using 6 percent albumin. No agglutination should be

C.B. Bub

Immunohematology, Volume 33 , ISSUE 4, 170–172

Article | 27-December-2020

The elucidation of a Kell-related autoantibody using ZZAP-treated red cells

A 61-year-old, nontransfused, Caucasian male was found to have a positive direct antiglobulin test (DAT) and an autoantibody in his serum prior to total hip replacement. Autoabsorption with the patient's ZZAP-treated red cells failed to absorb the autoantibody, giving a clue to its possible specificity, which was subsequently found to be Kell-system related. In addition, his red cells were found to have a slightly weakened expression of Kell system antigens. The patient was fit and healthy

Graham P. Rowe, Guiseppe G. Tozzo, Joyce Poole, Yew Wah Liew

Immunohematology, Volume 5 , ISSUE 3, 79–82

Article | 16-November-2020

A method to detect McLeod phenotype red blood cells

carrier females (obligate heterozygotes) is even more difficult because only a minor subpopulation of RBCs may express the weakened Kell phenotype. RBCs from 12 sets of mother/son or father/daughter pairs were tested by standard hemagglutination tube tests and by flow cytometry using both monoclonal and polyclonal Kell system antibodies. Monoclonal anti-K14 (G10) in tests with RBCs from McLeod males reacted ± by hemagglutination (control cells 2+) and had a median fluorecence of 6–11 by

Ragnhild Øyen, Marion E. Reid, Pablo Rubinstein, Harold Ralph

Immunohematology, Volume 12 , ISSUE 4, 160–163

Article | 14-December-2020

Typing of normal and variant red cells with ABO, Rh, and Kell typing reagents using a gel typing system

In 1989 Lapierre et al. described a novel method of detecting agglutination reactions by the use of a Sephadex (DiaMed ID Typing System) gel held in a microtube. This report examines the use of gels containing ABO, Rh, and Kell system specific antibodies. The anti-A and -B were monoclonal reagents; anti-A,B, and those for the Rh and Kell systems were polyclonal. Five hundred and fifty-one tests performed for the ABO system detected all but the most weakly reacting variants, a detection rate

Don Tills, Derek J. Ward, Dieter Josef

Immunohematology, Volume 7 , ISSUE 4, 94–97

Case report | 01-December-2019

Red blood cell phenotype matching for various ethnic groups

phenotype distribution of Rh, Kell, Kidd, Duffy, and MNS. A random subsample of 800 donor phenotypes was then evaluated for the probability of obtaining an exact match with respect to phenotype with a randomly selected patient from a particular ethnic group. Overall, there was a greater than 80 percent probability of finding an exact donor-recipient match for the K/k alleles in the Kell system. The probability ranged from 3 percent to 38 percent, depending on the ethnicity and disparities in phenotypic

Karafa S.W. Badjie, Craig D. Tauscher, Camille M. van Buskirk, Clare Wong, Sarah M. Jenkins, Carin Y. Smith, James R. Stubbs

Immunohematology, Volume 27 , ISSUE 1, 12–19

Article | 26-October-2020

EDTA/glycine-acid versus chloroquine diphosphate treatment for stripping Bg antigens from red blood cells

EDTA/glycine-acid (EGA) has been reported to remove IgG-bound antibodies from red blood cells (RBCs) and to denature Kell system and Era antigens. EGA-treated RBCs were tested in parallel with chloroquine diphosphate (CDP)-treated RBCs to evaluate whether EGA would remove Bg antigens from RBCs as efficiently as CDP. Fifty-seven serum/plasma samples containing known Bg antibodies were tested with untreated Bg+ RBCs, EGA-treated Bg+ RBCs, and CDP-treated Bg+ RBCs by an indirect antiglobulin test

Kayla D. Champagne, Peggy Spruell, Jane Chen, Leslie Voll, Gloria Schlanser

Immunohematology, Volume 15 , ISSUE 2, 66–68

Report | 16-October-2019

Rh and Kell blood group antigen prevalence in a multi-ethnic cohort in Nigeria: implications for local transfusion service

Antigens belonging to the Rh and Kell blood group systems are of major clinical significance because of their immunogenicity and the potential of their consequent antibodies to cause in vivo destruction of exogenous red blood cells (RBCs). Despite the widespread use of transfusion, there are sparse data on the prevalence of Rh and Kell system antigens and their ethnic variability in Nigeria. The objective of this study was to determine the prevalence of the five major Rh (D, C, c, E, e) and

Ademola Samson Adewoyin, Grace Ming Lee, Titilope Adenike Adeyemo, Omolade Augustina Awodu

Immunohematology, Volume 34 , ISSUE 2, 61–65

Article | 15-April-2020

Efficacy of murine monoclonal antibodies in RBC phenotyping of DAT-positive samples

Determining the phenotype of patient RBCs that are positive by the DAT may prove problematic. Antigen typing of RBCs coated with IgG requires direct agglutinating reagents or chemical treatment (such as chloroquine diphosphate [CDP] or citric acid) to remove sufficient IgG to permit testing with IAT-reactive reagents. The citric acid elution method is commonly used in the United States; however,antigens in the Kell system are altered to the extent that they may appear to be absent by this

Edmond Lee, Kevin Hart, Gordon Burgess, Gregory R. Halverson, Marion E. Reid

Immunohematology, Volume 22 , ISSUE 4, 161–165

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