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  • Journal Of Nematology

 

research-article | 06-November-2020

Morphological and molecular analyses of a Meloidogyne mali population with high intragenomic rRNA polymorphism

those in the basal clades. In the present study, we reported that a M. mali population was imported from Japan in Ningbo Port, China, with high intragenomic rRNA polymorphisms. We describe this population by detailed morphology and molecular phylogeny, and compare it with other populations in GenBank by mitochondrial COI haplotype network analysis. Materials and methods Isolation and morphological observation of nematodes Roots and a little soil and rhizosphere medium associated with maple (Acer

Jianfeng Gu, Yiwu Fang, Lele Liu

Journal of Nematology, Volume 52 , 1–11

research-article | 30-November-2019

Characterization of a population of Pelodera strongyloides (Nematoda: Rhabditidae) associated with the beetle Lucanus ibericus (Coleoptera: Lucanidae) from Georgia

. In the present study, a Georgian population of P. strongyloides recovered for the first time from L. ibericus and was fully characterized by morphology and morphometrics. Furthermore, the PCR amplification and sequencing of the D2 to D3 expansion domains of the 28S rRNA gene, the ITS, and the mitochondrial COI were carried out. The phylogenetic relationships of P. strongyloides from Georgia to other Pelodera species and Rhabditidae were also reconstructed. Materials and methods Nematode

O. Gorgadze, A. Troccoli, E. Fanelli, E. Tarasco, F. De Luca

Journal of Nematology, Volume 52 , 1–12

research-article | 24-April-2020

Mitochondrial COI gene is valid to delimitate Tylenchidae (Nematoda: Tylenchomorpha) species

with mitochondrial COI gene. New sequences original to this study are indicated in bold. Branch support is PP value in BI analysis. Figure 5: The maximum likelihood tree interfered on the mitochondrial COI gene. New sequences original to this study are indicated in bold. Branch support is BS value from ML analysis. Although COI phylogeny was unable to reject rRNA phylogenies with full confidence, several COI placements were incongruent with rRNA phylogenies with moderate support in ML

Mengxin Bai, Xue Qing, Kaikai Qiao, Xulan Ning, Shun Xiao, Xi Cheng, Guokun Liu

Journal of Nematology, Volume 52 , 1–12

Research Article | 15-August-2017

Description and Distribution of Three Criconematid Nematodes from Hangzhou, Zhejiang Province, China

Populations of Criconemoides parvus, Discocriconemella hengsungica, and Discocriconemella limitanea, isolated in Hangzhou, China from the rhizosphere soil of woody perennials were characterized morphologically and molecularly. The morphometric data of the Chinese populations were compared with populations from other regions of the world. DNA barcoding with the mitochondrial COI gene confirmed conspecificity of Chinese and Costa Rican populations of D. limitanea. Phylogenetic assessment using a

Maria Munawar, Thomas O. Powers, Zhongling Tian, Timothy Harris, Rebecca Higgins, Jingwu Zheng

Journal of Nematology, Volume 50 , ISSUE 2, 183–206

Research Article | 17-October-2018

Characterization of Meloidogyne indica (Nematoda: Meloidogynidae) Parasitizing Neem in India, with a Molecular Phylogeny of the Species

juveniles, males and females were carried out by light compound and scanning electron microscopy. Gross morphology and measurements were found consistent with the original description of M. indica infecting citrus by Whitehead (1968). The neem population was found to infect and reproduce on citrus. Additionally, evolutionary relationship was deduced by Maximum likelihood method using ITS rRNA, D2D3 expansion segment of 28S rRNA and mitochondrial COI sequences. Phylogenetic analyses based on these

Victor Phani, Satyapal Bishnoi, Amita Sharma, Keith G. Davies, Uma Rao

Journal of Nematology, Volume 50 , ISSUE 3, 387–398

Research Article | 26-September-2018

Occurrence of Sheraphelenchus sucus (Nematoda: Aphelenchoidinae) and Panagrellus sp. (Rhabditida: Panagrolaimidae) Associated with Decaying Pomegranate Fruit in Italy

rRNA gene, D2–D3 expansion domains of the 28S rDNA, the ITS region, and the partial mitochondrial COI were carried out. Sequences of the 18S rRNA gene, the D2–D3 domains, and the ITS were analyzed using several methods for inferring phylogeny to reconstruct the relationships among Sheraphelenchus and Bursaphelenchus species. The bacterial feeder Panagrellus sp. was characterized at the molecular level only. The D2–D3 expansion domains and ITS sequences of this Italian panagrolaimid were determined

ELENA FANELLI, ALBERTO TROCCOLI, NICOLA VOVLAS, GIANLUCA SCARCIA, ANNAMARIA MINCUZZI, SIMONA M. SANZANI, ANTONIO IPPOLITO, FRANCESCA DE LUCA

Journal of Nematology, Volume 49 , ISSUE 4, 418–426

research-article | 30-November-2018

A COI DNA barcoding survey of Pratylenchus species in the Great Plains Region of North America

temporary slides and smashed in an 18 µl drop of sterile deionized distilled water using a sterile, transparent micropipette tip. Smashed specimens were transferred to PCR reaction microfuge tubes and stored at −20°C until PCR amplification. PCR primers and amplification conditions The mitochondrial COI gene was amplified by PCR using primer sets of COI-F7b-Prat (F7bP) (5′-GGDTGRACWTTHTAYCCNCC-3′) developed by the UNL nematology laboratory, and COI-JB5 (5′-AGCACCTAAACTTAAAACATAATGAAAATG-3′) (Derycke

Mehmet Ozbayrak, Tim Todd, Timothy Harris, Rebecca Higgins, Kirsten Powers, Peter Mullin, Lisa Sutton, Thomas Powers

Journal of Nematology, Volume 51 , 1–21

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