Article | 01-June-2015
-based classifiers from a binary tree classification as a decision-making system. In our framework for molecular robot development, the system components of molecular robots and simple classifier rules were used to alleviate the computational burden. The design consists of a basic model that generates rule-based classifier gates in several binary tree and cancer classifications based on micro (mi)RNA expression. Simulation results showed that detection and classification were rapid using this system
Wibowo Adi,
Kosuke Sekiyama
International Journal on Smart Sensing and Intelligent Systems, Volume 8 , ISSUE 2, 944–965
Article | 15-April-2020
her serum. No potential donors were identified among family members or within the American Rare Donor Program;therefore,a unit of the patient’s RBCs was collected one week before her planned caesarian section. To improve our ability to supply blood for this patient in the future,molecular testing was performed. The patient was found to be homozygous for an RH haplotype in which a variant RHD*DAR, is linked to a variant RHCE*ceAR. The DAR-ceAR haplotype has been described in Dutch-African
Richard L. Haspel,
Dawn Michelle,
Richard M. Kaufman,
Sunitha Vege,
Connie M. Westhoff
Immunohematology, Volume 22 , ISSUE 3, 132–135
Review | 15-April-2020
chance of recombination, resulting in hybrid molecules that often carry one or more novel antigens. Some of the antigens in the MNS system result from a single nucleotide substitution. The MNS blood group system now consists of more than 40 distinct antigens. This review summarizes the molecular basis associated with some of the antigens in the MNS blood group system.
P. Palacajornsuk
Immunohematology, Volume 22 , ISSUE 4, 171–182
research-article | 19-February-2021
(Farjallah et al., 2008). Recent studies indicated morphological differences between Anisakis species (Chen and Shih, 2015; Sonko et al., 2019; Tunya et al., 2020). In addition, molecular tools allow the accurate identification of anisakid larvae by using sequences of the internal transcribed spacer (ITS) region of ribosomal DNA (D’amelio et al., 2010; Mattiucci and Nascetti, 2008).
In Vietnam, data on Anisakis nematodes are scarce. There have been a few reports on Anisakis larvae without morphological
Hoang Van Hien,
Bui Thi Dung,
Ha Duy Ngo,
Pham Ngoc Doanh
Journal of Nematology, Volume 53 , 1–9
research-article | 30-November-2020
the four subfamilies classified under the family Leptonchidae (Thorne, 1935), but its relationships with the others leptonchid genera have not been analyzed using molecular data yet, due to lack of DNA sequences of the Xiphinemellinae members. This study was conducted with the objectives to (i) characterize a population of Xiphinemella esseri (Chitwood, 1957), recently collected in Florida, with molecular and morphological taxonomic approach, and (ii) provide new insights in the phylogeny and
Sergio Álvarez-Ortega,
Sergei A. Subbotin,
Renato N. Inserra
Journal of Nematology, Volume 53 , 1–9
Case report | 01-December-2019
Whether or not patients whose red blood cells (RBCs) carry certain weak D types produce anti-D, and if they do whether it is allo- or autoanti-D, remains controversial. The aim of this study was to determine the serologic features of anti-D in individuals expressing a weak D other than type 1 or type 2 and to assess whether the anti-D was an allo- or autoantibody. Serologic D typing and molecular analyses were performed on 748 individuals. Serologic characterization of anti-D
Bach-Nga Pham,
Michèle Roussel,
Dominique Gien,
Maryline Ripaux,
Carine Auxerre,
Pierre-Yves Le Pennec,
Christine Andre-Botte
Immunohematology, Volume 29 , ISSUE 2, 55–62
research-article | 30-November-2019
and processed to glycerin by the formalin glycerin method (Golden, 1990; Hooper, 1970). Photomicrographs of the specimens were made with a Nikon Eclipse Ni compound microscope using a Nikon DS-Ri2 camera. Measurements were made with an ocular micrometer on a Leica WILD MPS48, Leitz DMRB compound microscope. All measurements are in micrometers unless otherwise stated.
The molecular identification was performed using DNA extracted from single nematodes as template in PCR reactions. The internal
Zafar A. Handoo,
Andrea M. Skantar,
Mihail R. Kantor,
Saad L. Hafez,
Maria N. Hult
Journal of Nematology, Volume 52 , 1–5
research-article | 11-January-2021
den Berg et al. (2014) provided a detailed molecular and morphological characterization of several populations identified as P. nanus from South Africa and California, USA. This study showed that the populations identified as P. nanus might indeed contain two sibling species (molecular types A and B), which were well separated using molecular criteria, but had very similar morphometrics. These authors also noticed intraspecific variation in shapes of lip region and tail. Thus, the designation of
Sergei A. Subbotin,
Guiping Yan,
Mihail Kantor,
Zafar Handoo
Journal of Nematology, Volume 52 , 1–7
research-article | 30-November-2018
Oscheius as well the two monophyletic species groups within the latter.
Molecular data of Oscheius sensu lato species, many of them described during the last years, have been matter of analyses by several authors (Félix et al., 2001; Darby et al., 2011; Darsouei et al., 2014; Campos-Herrera et al., 2015; Torrini et al., 2015; Tabassum et al., 2016; Lima de Brida et al., 2017; Valizadeh et al., 2017; Zhou et al., 2017), resulting in the confirmation of the monophyly of the Insectivorus- and the
Joaquín Abolafia,
Reyes Peña-Santiago
journal of nematology, Volume 51 , 1–21
research-article | 30-November-2020
obvious symptoms on the potato especially for the first months of field infestation. Then it damages the roots and reduces the production yields. Damages can appear to be symptoms of water stress or mineral deficiencies.
Morphological identification of Globodera species is an initial means of differentiation of Globodera species that are morphologically and morphometrically closely related that ultimately requires molecular confirmation. Molecular diagnosis by the polymerase chain reaction (PCR) test
A. Hajjaji,
R. Ait Mhand,
N. Rhallabi,
F. Mellouki
Journal of Nematology, Volume 53 , 1–8
research-article | 30-November-2018
, 1980) in the order of Tylenchida (Thorne, 1949). Siddiqi (2000) considered the family Paurodontidae a junior synonym of Sphaerulariidae, since the genera included in these families are morphologically similar and have similar life cycles. However, there is not enough available molecular data to support these statements. Biologically, in Paurodontidae, a fungus-feeding generation is well known and nothing is known about entomoparasitic forms, whereas in Sphaerulariidae, an entomoparasitic form is
Mehrab Esmaeili,
Ramin Heydari,
Ahmad Kheiri,
Weimin Ye
Journal of Nematology, Volume 51 , 1–12
research-article | 30-November-2020
et al., 2012, 2016; Zhao, 2009, 2011).
The present work focuses on morphological and molecular characterisation of the Tripylina gorganensis found during an ecological study, on the impact of natural disturbance on soil nematode communities’ structures in a natural beech forest (Fagus sylvatica L.) in the Slovak Republic. Re-examination of the paratypes of T. gorganensis has pointed out the necessity to redescribe this species. T. gorganensis happens to be another European species following the
Marek Renčo,
Katarzyna Rybarczyk-Mydłowska,
Łukasz Flis,
Magdalena Kubicz,
Grażyna Winiszewska
Journal of Nematology, Volume 53 , 1–10
Article | 03-December-2017
, Samastipur grouped with M. oryzae and M. graminis. Molecular phylogenetic analysis using internal transcribed spacer (ITS) suggested that in spite of morphological differences, these populations belonged to M. graminicola.
RAJAN SALALIA,
R. K. WALIA,
VISHAL SINGH SOMVANSHI,
PUNEET KUMAR,
ANIL KUMAR
Journal of Nematology, Volume 49 , ISSUE 3, 254–267
research-article | 28-April-2020
and cultivated soils of northwest Iran (East–West Azarbaijan and Kurdistan provinces) to explore the dorylaimid diversity of this region. Their study revealed that they belonged to one new and one known species. The objective of this work was to report Pungentus sufiyanensis n. sp. using morphology, morphometric, and molecular methods and provide new data about P. engadinensis.
Materials and methods
Extraction and processing of nematodes
Soil samples were collected from the rhizosphere of
Nasir Vazifeh,
Gholamreza Niknam,
Habibeh Jabbari,
Reyes Peña-Santiago
Journal of Nematology, Volume 52 , 1–12
Report | 26-October-2019
and maintain adequate rare inventory of each. Molecular red blood cell genotyping allows transfusion services to increase their availability of rare phenotypes for chronically transfused patients.
Lorena I. Aranda,
Linda A. Smith,
Scott Jones,
Rachel Beddard
Immunohematology, Volume 31 , ISSUE 4, 166–173
research-article | 26-March-2021
from vermicompost in Iran and presented the first molecular characterization of the species.
In South Africa the genus Butlerius was first reported in 1961 (Heyns, 1961) and are commonly found in soil samples (Dr Marais, ARC-PHP, personal communication). However, populations of the genus are very rarely formally reported in the country. Species of the genus are yet to be reported or described from South Africa. During sampling of compost matter from two different localities in Potchefstroom, North
Chantelle Girgan,
Gerhard Du Preez,
Hendrika Fourie,
Milad Rashidifard
Journal of Nematology, Volume 53 , 1–12
Report | 25-March-2020
Because of the scarcity of anti-Hy and anti-Joa, hemagglutination typing for the Dombrock blood group system antigens, Hy and Joa, is not feasible. The molecular bases associated with these antigens have been determined, making it possible to distinguish HY and JO from wild-type DO. This provides a tool to predict the probable phenotype of patients and to screen for antigen-negative donors. PCR-RFLP assays and a microchip assay were used to determine the frequency of HY and JO
Lilian Castilho,
Wilson Baleotti,
Edith Tossas,
Kim Hue-Roye,
Karina R. Ribeiro,
Christine Lomas-Francis,
Daisy Charles-Pierre,
Marion E. Reid
Immunohematology, Volume 24 , ISSUE 4, 135–137
Article | 04-December-2017
terminus observed in some specimens. The matrix code of P. haiduongensis sp. n. is: A2, B1, C4, D(1,3), E1, F(5,6), G(1,2), H(1,4); I(1,2,3,4), J1, K(1,2) according to Castillo and Vovlas (2007). The LSU–D2D3 segment and the ITS–rDNA region of this species were amplified and sequenced. The morphological characters and molecular phylogenetic analyses confirmed that this is a new species of the genus Pratylenchus in Vietnam.
THI DUYEN NGUYEN,
THI MAI LINH LE,
HUU TIEN NGUYEN,
THI ANH DUONG NGUYEN,
GRACIA LIEBANAS,
QUANG PHAP TRINH
Journal of Nematology, Volume 49 , ISSUE 3, 276–285
Report | 25-March-2020
The molecular background of variant forms of GYPB is not well studied in Brazilians of African descent. The present study was carried out to determine the molecular bases of the S–s– phenotype and the frequency of GYPB*S silent gene for the S–s+ phenotype in a blood donor population of African Brazilians. In this study, 165 blood samples from African Brazilians (Northeastern Brazil) who phenotyped as S–s– (n = 17) and S–s+ (n = 148) by
Ricardo Omoto,
Marion E. Reid,
Lilian Castilho
Immunohematology, Volume 24 , ISSUE 4, 148–153
Research Article | 23-May-2019
The molecular typing methods are used to identify specific genetic targets and relationships between microbial isolates. In order to understand clonal relatedness between the microbial strains, classic phenotypic methods are used in line with modern molecular biology techniques. The development of genetics, especially new techniques like molecular typing, have revolutionized microbial research. After 1970, the development techniques, especially those referring to DNA sequencing, established
Daria Artyszuk,
Tomasz Wołkowicz
Postępy Mikrobiologii - Advancements of Microbiology, Volume 57 , ISSUE 2, 179–193
research-article | 14-December-2020
Jacob, 2012, and Oscheius amsactae Ali, Pervez, Andrabi, Sharma and Verma, 2011 and Metarhabditis longicaudata Tabassum, Salma and Nasir, 2019. Most of these studies, however, have characterized the species morphologically and morphometrically. Regarding molecular analysis, several Internal Transcribed Spacer (ITS) rDNA sequences, obtained from M. amsactae isolated in India, Philippines, and Pakistan have been deposited in the GenBank, but none of the nematode specimens used to obtain the sequences
Aashaq Hussain Bhat,
Shreyansh Srivastava,
Aasha Rana,
Ashok Kumar Chaubey,
Ricardo A. R. Machado,
Joaquín Abolafia
Journal of Nematology, Volume 52 , 1–23
research-article | 30-November-2019
corms and roots of banana where they were likely to cause damage to the crop (Sikora et al., 2018). Phylogenetic analysis of Hoplolaimus spp. using D2–D3 expansion of 28 S and internal transcribed spacer (ITS1) ribosomal DNA sequences resolved the phylogeny of the genus and were useful in molecular identification of Hoplolaimus spp. (Bae et al., 2008). In addition, the PCR-RFLP method was applied by different researchers to evaluate the genetic diversity of Hoplolaimus spp. (Robbins et al., 2009
Mariette Marais,
Esther van den Berg,
Hendrika Fourie,
Milad Rashidifard
Journal of Nematology, Volume 52 , 1–12
Article | 26-October-2020
A chimeric individual possesses two or more genetically distinct cell populations. Although the chimerism may not be evident in all gene systems, various loci display greater numbers of alleles than genetically "normal" individuals. The proposita was referred for further laboratory investigation due to a rnixed-field ABO blood group reaction following routine antenatal testing. Various molecular (HLA class Il, ABO genotyping, and 10 short tandem repeat [STR] microsatellites) and
Nicole A. Mifsud,
Albert P. Haddad,
Cathie F. Hart,
Jennifer A. Condon,
Michael Swain,
Rosemary L. Sparrow
Immunohematology, Volume 15 , ISSUE 3, 100–104
research-article | 18-March-2020
isolated by the modified Baermann funnel technique for 24 hr. Permanent slides were prepared by heat-killed and fixed nematodes with FA 4:1 and ethanol-glycerin dehydration according to Seinhorst (1959) as modified by De Grisse (1969). Morphometrics, drawings, and light micrographs of nematodes were done with the aid of a Zeiss microscope equipped with a Zeiss AxioCam MRm CCD camera.
Molecular and phylogenetic analyses
DNA samples were prepared according to Li et al. (2008). Three sets of primers
Jianfeng Gu,
Munawar Maria,
Lele Liu,
Majid Pedram
Journal of Nematology, Volume 52 , 1–11
Original Research | 11-December-2017
and diagnostic features of Pseudacrobeles species and molecular sequence data from the D2-D3 regions of the 28S ribosomal DNA (rDNA) and ITS1-5.8S-ITS2 region of rDNA from the new species, which can be used as molecular barcode sequences.
Jiyeon Kim,
Taeho Kim,
Joong-Ki Park
Journal of Nematology, Volume 49 , ISSUE 2, 162–167
Research Article | 03-December-2018
Juvenile, female and male nematodes were discovered in wood chips of white pine Pinus strobus from Ashley Falls, MA. Initial observations suggested these nematodes might be PWN, but closer morphological and molecular characterization proved otherwise. Comparison of measured features with those in the literature indicated this nematode population had some unique characteristics. The specimens were identified as Bursaphelenchus antoniae Penas et al., 2006 based on 18S rDNA molecular sequence vs
Lynn K. Carta,
R. L. Wick
Journal of Nematology, Volume 50 , ISSUE 4, 473–478
Review | 18-October-2020
Marion E. Reid,
Agnes Hallie Lee
Immunohematology, Volume 16 , ISSUE 1, 1–6
Research Article | 03-December-2018
The 18S small subunit (SSU) ribosomal DNA sequence is one of the most useful molecular loci for identification and phylogeny reconstruction of agriculturally important nematodes. Various pairs of universal primers have been developed in the past to amplify short and long nematode sequences. However, certain nematode taxa were not readily amplified and/or sequenced with the existing primer tools. Frequently, the center region of a roughly 1,000 nucleotide segment would be lost. Therefore new
L. K. Carta,
S. Li
Journal of Nematology, Volume 50 , ISSUE 4, 533–542
research-article | 30-November-2020
. sphaerocephalum were deposited in GenBank under the accession numbers MW254991-MW254992 (18S rDNA) and MW256823-MW256824 (28S rDNA), respectively.
Results and discussion
Morphological characterization
The morphological and molecular analyses confirmed that the species was M. sphaerocephalum. Measurements of M. sphaerocephalum in this study are in agreement with the measurement of M. sphaerocephalum in Geraert (2010) and Nguyen et al. (2019) (Table 1). Females of M. sphaerocephalum are characterized by
Ebrahim Shokoohi
Journal of Nematology, Volume 53 , 1–5
Article | 03-December-2017
Plant-parasitic nematodes such as Longidorus euonymus and Helicotylenchus multicintctus are species widely distributed in central Europe as well as in Mediterranean area. In Greece, both species have been previously reported but no morphometrics or molecular data were available for these species. Nematode surveys in the rhizosphere of grapevines in Athens carried out in 2016 and 2017, yielded a Longidorus species identified as Longidorus euonymus. Similarly, a population of Helicotylenchus
EMMANUEL A. TZORTZAKAKIS,
CAROLINA CANTALAPIEDRA-NAVARRETE,
PABLO CASTILLO,
JUAN E. PALOMARES-RIUS,
ANTONIO ARCHIDONA-YUSTE
Journal of Nematology, Volume 49 , ISSUE 3, 233–235
Review | 01-December-2019
The Lutheran blood group system currently consists of 20 antigens that have been assigned ISBT numbers. Of these, all but LU7 have been associated with one or more nucleotide changes in LU. The purpose of this study was to determine the molecular basis associated with the LU:–7 phenotype. We obtained a stored sample from one proband with this phenotype and sequenced LU. Using genomic DNA, exons 1 through 15, and their flanking intronic regions, of LU were amplified by polymerase-chain
Kim Hue-Roye,
Marion E. Reid
Immunohematology, Volume 28 , ISSUE 4, 130–131
Original Paper | 09-October-2019
Alloimmunization to red blood cell antigens is unpredictable and poorly understood. Patients who are negative for highincidence antigens (HIAs) are at risk for developing the corresponding antibodies. Molecular methods can easily predict the lack of an antigen and thus, the risk of an individual to become immunized. We examined the prevalence and risk factors for HIA alloimmunization in patients at risk based on genotyping results. Genotyping using a molecular method (HEA BeadChip&trade
Patricia A.R. Brunker,
Keerthana Ravindran,
R. Sue Shirey
Immunohematology, Volume 33 , ISSUE 1, 9–14
Case report | 01-December-2019
Rh discrepancies are a problem during routine testing because of partial D or weak D phenotypes. Panels of monoclonal antibodies (MoAb) are being developed to identify D variants such as partial D and weak D when there are anomalous D typing results; however, molecular characterization offers a more specific classification of weak and partial D. The weak D and partial D phenotypes are caused by many different RHD alleles encoding aberrant D proteins, resulting in distinct serologic phenotypes
Débora Castilho Credidio,
Jordão Pellegrino Jr.,
Lilian Castilho
Immunohematology, Volume 27 , ISSUE 1, 6–11
Research Article | 03-September-2018
Longidorus azarbaijanensis n. sp. is described and illustrated using morphological and molecular data. It was recovered in West Azarbaijan province, northwestern Iran, from the rhizospheric soil of foxtail weed. The new species is characterized by having 5.4 to 6.8 mm long females, offset, anteriorly flat lip region and separated from the rest of the body by a shallow constriction, funnel-shaped amphidial pouches, the guiding ring at 23 to 27 µm from the anterior end, 73 to 81 and 44 to 50 µm
Farshad Gharibzadeh,
Ebrahim Pourjam,
Majid Pedram
Journal of Nematology, Volume 50 , ISSUE 2, 207–218
Research paper | 06-February-2018
Alexandra A. Gorbunova,
Irina V. Kudryashova,
Anna O. Manolova,
Margarita R. Novikova,
Mikhail Yu. Stepanichev,
Natalia V. Gulyaeva
Acta Neurobiologiae Experimentalis, Volume 77 , ISSUE 3, 244–253
research-article | 24-April-2020
, 20 km north of the town Lichtenburg (North-West Province, South Africa). In-depth investigations revealed that it belongs to an unknown species, being described herein as C. driekieae n. sp. Therefore, the present study aims to characterize this species using both traditional and molecular techniques and discuss its phylogenetic affinities using SSU and LSU markers.
Materials and methods
Nematode extraction and morphological studies
Nematodes were extracted from the soil using the modified
Milad Rashidifard,
Gerhard Du Preez,
Joaquín Abolafia,
Majid Pedram
Journal of Nematology, Volume 52 , 1–10
research-article | 17-April-2019
record of this species in Russia.
Materials and Methods
Nematodes were extracted from the soil samples using a modification of the decanting and sieving method (Flegg, 1967). For morphological studies, the nematodes were killed with hot water, fixed in 5 % formalin solution, and mounted in glycerin slides using the Seinhorst technique (Seinhorst, 1959).
For molecular studies, nematodes were fixed with 96% ethanol. Their total DNA was extracted using the K-Sorb kit (Syntol LLC, Russia) according to
Sergei Tabolin,
Irina Markina
Journal of Nematology, Volume 51 , 1–4
research-article | 30-November-2020
Cristiano Bellé,
Paulo Sergio dos Santos,
Tiago Edu Kaspary
Journal of Nematology, Volume 53 , 1–4
research-article | 06-November-2020
, 1991) and populus in Henan province (Li and Yu, 1991), both descriptions lack detailed morphological or molecular data, and neither citrus nor populus are known to be the host of M. mali. Thus, the presence of M. mali in China remains questionable, as does the suitability of citrus and populus as hosts. Due to its wide host and broad distribution, M. mali was placed on the EPPO Alert List in 2014.
Intragenomic polymorphisms have been found in various plant–parasitic nematode taxa, including genera
Jianfeng Gu,
Yiwu Fang,
Lele Liu
Journal of Nematology, Volume 52 , 1–11
research-article | 30-March-2020
time in Italy, and, in particular, associated with C. sativus corms. Morphometrical and molecular characterization of the nematode is given. In addition, a review of nematodes associated with plants of C. sativus worldwide is provided.
Material and methods
Collecting corms and nematodes extraction
In September 2018, 20 corms of Crocus sativus were collected by the Plant Protection Service of Lazio Region on the farm “Arte Zafferano” located in Terracina (Latina, Lazio, Central Italy), in the
Giulia Torrini,
Agostino Strangi,
Stefania Simoncini,
Maria Luppino,
Pio Federico Roversi,
Leonardo Marianelli
Journal of Nematology, Volume 52 , 1–11
research-article | 30-November-2018
population of Xiphinema lapidosum was identified in association with Olea europaea subsp. europaea L. Lamberti et al. (1993a, 1993b) and Roca and Bravo (1993) described female and male stages of X. histriae and X. lapidosum, respectively, but in both species no juvenile stages were detected and described. The objectives of this study were: (i) to provide updated morphological descriptions of juvenile stages of X. histriae and X. lapidosum, (ii) to characterize the molecular data of both species using the
Ruihang Cai,
Antonio Archidona-Yuste,
Carolina Cantalapiedra-Navarrete,
Juan E. Palomares-Rius,
Jingwu Zheng,
Pablo Castillo
Journal of Nematology, Volume 51 , 1–21
Research Article | 03-September-2018
Nothotylenchus andrassy n. sp. is described and illustrated from moss (Sphagnum sp.) based on morphology and molecular analyses. Morphologically, this new species is characterized by a medium body size, six incisures in the lateral fields, and a delicate stylet (8–9 µm long) with clearly defined knobs. Pharynx with fusiform, valveless, non-muscular and sometimes indistinct median bulb. Basal pharyngeal bulb elongated and offset from the intestine; a long post-vulval uterine sac (55% of vulva to
Parisa Jalalinasab,
Mohsen Nassaj Hosseini,
Ramin Heydari
Journal of Nematology, Volume 50 , ISSUE 2, 219–228
research-article | 30-November-2019
, covered vulva by a protuding anterior vulvar lip, presence of a long post-uterine sac, a narrow and elongated tail with a clindroid and rounded terminus (Opperman, 1995).
Currently, DNA sequences of segment D2-D3, Internal Transcribed Spacer-(ITS) of ribosomal RNA and Cytochrome oxidase subunit I-COI of mitochondrial DNA have been obtained for B. cocophilus in coconut palm and deposited in public molecular databases, which are used as a reference for the identification of the nematode (Ye et al., 2007
Greicy Andrea Sarria,
Donald Riascos-Ortiz,
Hector Camilo Medina,
Yuri Mestizo,
Gerardo Lizarazo,
Francia Varón De Agudelo
Journal of Nematology, Volume 52 , 1–12
Article | 21-April-2020
daughter was also studied. Sequence analysis revealed a substitution of 508C>T in exon 4 of DAF in the proband. The proband’s daughter was heterozygous for 508C/T. This study describes an Inab phenotype in which the 508C>T nonsense mutation is predicted to change arginine at amino acid residue 136 to a stop codon. This change is in SCR 3 of DAF . This study reports on the molecular basis of a new proband with the Inab phenotype who had no history of intestinal disorders.
Kim Hue-Roye,
Vivien E. Powell,
Gita Patel,
Debra Lane,
Mariska Maguire,
Amy Chung,
Marion E. Reid
Immunohematology, Volume 21 , ISSUE 2, 53–55
Research Article | 03-December-2018
The root-lesion nematode of the genus Pratylenchus Filipjev (1936) has a worldwide distribution and cause severe production constraints on numerous important crops. In 2013-14, during a survey of the apple nurseries and orchards in center of Tunisia (Kairouan, Zaghouan, Monastir and Kasserine), 70 different roots and soil samples were collected. The populations of root-lesion nematode were identified on the basis of their morphological and morphometric characters, and by molecular methods
Noura Chihani-Hammas,
Lobna Hajji-Hedfi,
Hajer Regaieg,
Asma Larayedh,
Ahmed Badiss,
Yu Qing,
Horrigue-Raouani Najet
Journal of Nematology, Volume 50 , ISSUE 4, 579–586
Research Article | 03-September-2018
During 2008 to 2016 in several nematological surveys in the Tepeaca Valley, Puebla, Mexico, carrot cyst nematode, Heterodera carotae was found parasitizing carrots, Daucus carota. The nematode was present in 61% of the sampled fields with high population densities, causing severe carrot yield losses in the Tepeaca Valley. The aim of this work was to study morphology, morphometrics, host range, and molecular characterization of the nematode. The morphological and morphometric characterization
Ilia Mariana Escobar-Avila,
Edgar Óliver López-Villegas,
Sergei A. Subbotin,
Alejandro Tovar-Soto
Journal of Nematology, Volume 50 , ISSUE 2, 229–242
research-article | 30-November-2020
.) murithi (Altherr, 1950) as follow.
From L. (E.) deliblatica by a longer body (788-874 vs 602-700 μm), slightly longer odontostyle (32-33 vs 26.8-31.4 μm) and odontophore (33-38 vs 22.4-30.0 μm), narrower tail tip (vs wider) and almost slender spiclues (vs with deep hollow).
From L. (E.) murithi by a cephalic region separated from the rest body by a sharp constriction (vs not), shorter odontostyle (32-33 vs 40-44 μm) and tail tip not remarkably narrowing (Andrássy, 2009).
Molecular phylogenetic
Fariba Heydari,
Mohammad Reza Atighi,
Ebrahim Pourjam,
Majid Pedram
Journal of Nematology, Volume 53 , 1–11
Article | 03-December-2017
The taxonomy and the systematics of the genus Makatinus are discussed by means of the characterization of its morphological pattern and the first molecular (D2–D3 expansion segments of 28S rDNA) analysis of a representative of this taxon, Makatinus crassiformis from Costa Rica. The presence of two or more pairs of male ad-cloacal genital papillae is the most characteristic autapomorphy of the genus, but the status of its species on this concern differ among them. Both morphological and
REYES PENA-SANTIAGO,
INGRID VARELA
Journal of Nematology, Volume 49 , ISSUE 3, 245–253
Article | 26-October-2020
Accurate blood group antigen typing of red blood cells with a positive direct antiglobulin test or from a recently transfused patient has been a long-standing problem. To overcome this problem, we evaluated the feasibility of using somatic cells as a source of DNA for molecular genotyping. Two sources of cells that could be obtained by noninvasive procedures were chosen for analysis: urine samples, which were already available in the clinical laboratory, and buccal epithelial cells collected
Marion E. Reid,
Maria J. Rios,
Kevin L. Cash,
Annie M. Strupp,
Joan M. Uehlinger
Immunohematology, Volume 15 , ISSUE 2, 61–65
research-article | 24-April-2020
, 1993). Descriptions have been based on morphology and host preference and Trichuris from geomyid hosts have never been sequenced (Eberhardt et al., 2019). The aim of this study was to serve as the first molecular report for T. fossor. The 18S rRNA gene was sequenced for four specimens identified putatively as T. fossor collected from separate Thomomys (western pocket gophers) species hosts.
Materials and methods
Thomomys bulbivorus (Richardson, 1829; Brandt, 1855) were salvaged from trappers
Malorri R. Hughes,
Deborah A. Duffield,
Dana K. Howe,
Dee R. Denver
Journal of Nematology, Volume 52 , 1–6
Article | 21-July-2017
QING YU,
WEIMIN YE,
TOM POWERS
Journal of Nematology, Volume 48 , ISSUE 3, 203–213
original-paper | 31-October-2019
, V. fluvialis, and V. alginolyticus are reported as the causative agents of vibriosis in shrimp (de la Pena et al. 1993; Karunasagar et al. 1994; Lee et al. 1996; Lightner 1996; Austin and Zhang 2006; Chatterjee and Halder 2012). No molecular level study has so far been conducted to precisely identify the causal agent of vibrio sis in shrimp in Bangladesh and the required effective management for this worrisome disease.
A number of antibiotics and chemotherapeutic agents have been used in shrimp
MD. ABDUL HANNAN,
MD. MAHBUBUR RAHMAN,
MD. NURUNNABI MONDAL,
DEB SUZAN CHANDRA,
GAZLIMA CHOWDHURY,
MD. TOFAZZAL ISLAM
Polish Journal of Microbiology, Volume 68 , ISSUE 4, 429–438
Report | 25-March-2020
Molecular diagnostics is the fastest growing area of clinical laboratory medicine. The ability to rapidly amplify genes of bacterial, viral, or human origin, and the development of DNA array platforms, are driving a technology revolution in the clinical laboratory. A DNA-based testing approach is particularly applicable to blood bank and transfusion medicine for rapid, cost-effective antigen typing. Experience with DNA-based methods during the past decade has shown that these
Connie M. Westhoff
Immunohematology, Volume 24 , ISSUE 4, 190–195
research-article | 30-November-2019
were prepared according to Taylor and Netscher (1974). The determination of the esterase profile was made according to Carneiro and Almeida (2001), using 20 female.
For molecular identification, the D2 to D3 region of 28S rDNA segment was amplified and sequenced using the primers D2A (5′-ACAAGTACCGTGAGGGAAAGTTG-3′) and D3B (5′-TCGGAAGGAACCAGCTACTA-3′) (De Ley et al., 1999) and ITS primers with VRAIN2F (5′-CTTTGTACACACCGCCCGTCGCT-3′) and VRAIN2R (5′-TTTCACTCGCCGTTACTAAGGGAATC-3′) (Vrain et al., 1992
Francisco Jorge Carlos Souza Junior,
Mayara Castro Assunção
Journal of Nematology, Volume 52 , 1–4
Article | 21-July-2017
MEHRAB ESMAEILI,
RAMIN HEYDARI,
MOZHGAN ZIAIE,
JIANFENG GU
Journal of Nematology, Volume 48 , ISSUE 1, 34–42
Report | 25-March-2020
molecular testing, popular because they analyze multiple nucleotides in a single assay and have a high-throughput potential. This review briefly describes the principles of PCR-based assays that are commonly used in transfusion medicine.
Kim Hue-Roye,
Sunitha Vege
Immunohematology, Volume 24 , ISSUE 4, 170–175
Article | 04-December-2017
Abstract: Anguillonema amolensis n. sp. is described and illustrated based on its morphological, morphometric, and molecular characters. The new species is characterized by its 575 to 820 mm long and wide body (body width at vulva = 30 to 59 mm), irregularly ventrally curved after fixation, five to six lines in lateral fields, 6.0 to 7.5 mm long stylet with small rounded knobs, pharynx lacking median bulb, pharyngo-intestinal junction anterior to nerve ring and excretory pore, females with
MAHYAR MOBASSERI,
MAJID PEDRAM,
EBRAHIM POURJAM
Journal of Nematology, Volume 49 , ISSUE 3, 286–294
research-article | 31-August-2020
Chunhui Ni,
Shuling Zhang,
Huixia Li,
Yonggang Liu,
Wenhao Li,
Xuefen Xu,
Zhipeng Xu
Journal of Nematology, Volume 52 , 1–2
Research Article | 26-September-2018
Two different nematode species were recovered from pomegranate decaying fruit in two localities in Southern Italy: the mycetophagus nematode Sheraphelenchus sucus and a bacterial feeder nematode belonging to the Panagrolaimidae (Rhabditida) family. Morphometrics of the Italian population of S. sucus closely resemble that of the type population, whereas some differences were found when compared with another population from Iran. Molecular characterization of the Italian S. sucus using the 18S
ELENA FANELLI,
ALBERTO TROCCOLI,
NICOLA VOVLAS,
GIANLUCA SCARCIA,
ANNAMARIA MINCUZZI,
SIMONA M. SANZANI,
ANTONIO IPPOLITO,
FRANCESCA DE LUCA
Journal of Nematology, Volume 49 , ISSUE 4, 418–426
Article | 14-October-2020
MNS antigens are carried on glycophorin A (GPA), glycophorin B (GPB), or their variants. Antigens at the N-terminus of GPA are sensitive to cleavage by ficin, papain, and trypsin but are resistant to α-chymotrypsin. Antigens at the N-terminus of GPB are sensitive to cleavage by ficin, papain, and α-chymotrypsin but are resistant to trypsin treatment. These characteristics have been used to aid in the identification of blood group alloantibodies. Recent molecular analyses have
Marion E. Reid,
Jill Storry
Immunohematology, Volume 17 , ISSUE 3, 76–81
research-article | 30-November-2019
natural forests of Mazandaran province in north Iran. The comparisons with two currently valid species under the genus revealed that it belongs to an unknown species, described herein as Oostenbrinkia pedrami n. sp. The molecular phylogenetic analysis for the new species, as the first representative of the family, was also performed.
Materials and methods
Sampling, nematode extraction, mounting, and drawing
Several soil samples were collected from natural forests and grasslands of Mazandaran
Farahnaz Jahanshahi Afshar
Journal of Nematology, Volume 52 , 1–7
research-article | 30-November-2020
, and Pilosocereus gounellei in the municipality of Iguatu, CE (6° 25′18.7″S 39° 19′57.8″W). The samples were processed to extract the nematodes from the roots (Coolen and D´Herde, 1972) and soil (Jenkins, 1964).
For morphological characterization, the specimens were killed in a water bath (55°C) and fixed in formalin-acetic acid (Goodey, 1957). The morphometric characteristics and De Man (1880) (V%, a, b, b′, c, and c′) were obtained from adult females (Castillo and Vovlas, 2007).
The molecular
Francisco Jorge Carlos Souza Junior,
Mayara Castro Assunção
Journal of Nematology, Volume 53 , 1–5
research-article | 06-November-2020
. Species identification using morphological characters alone is very difficult and does not give correct results. A combination of both morphological and molecular approaches is very crucial for proper and accurate identification (Subbotin et al., 2015). The use of mitochondrial DNA cytochrome c oxidase subunit I (mtCOI) gene, ITS region, and D2-D3 expansion segments of 28S rDNA region sequences are trending today in molecular and phylogenetic studies (Hajibabaei et al., 2007; Subbotin et al., 2007
Rose Mwesige,
Eun-Hwa Kim,
Eun-Hyung Park,
Hyoung-Rai Ko
Journal of Nematology, Volume 52 , 1–12
research-article | 30-March-2020
Globally, cyst nematodes species cause serious damage to a wide variety of economically important crops. The need for information on cyst-forming nematode species has been instrumental in stimulating growth of nematology worldwide. The cyst nematode group currently contains eight genera, with a total of 121 valid species (Handoo and Subbotin, 2018). The general morphology and molecular taxonomy and phylogeny of cyst nematodes have been given in detail in two recent review articles (Baldwin and
Andrea M. Skantar,
Zafar A. Handoo,
Mihail R. Kantor,
Lynn K. Carta,
Jamal Faghihi,
Virginia Ferris
Journal of Nematology, Volume 52 , 1–8
Research Article | 17-October-2018
Labrys filiformis n. sp., the second species of the rare genus Labrys, was recovered from natural forests of Gilan province and is described based upon morphological and molecular characters. The new species is characterized by its smooth cuticle under light microscopy, lateral field with two incisures forming a single plain band, lip region continuous with body contour, dorso-ventrally flattened and forming four poorly prominent lobes, having a dorso-ventrally narrower protuberant labial plate
Yousef Panahandeh,
Joaquín Abolafia,
Ebrahim Pourjam,
Robin M. Giblin-Davis,
Farahnaz Jahanshahi Afshar,
Majid Pedram
Journal of Nematology, Volume 50 , ISSUE 3, 343–354
Short Communication | 28-June-2017
The paper concerns the fungus Phoma complanata, isolated for the first time in Poland, from the roots and umbels of angelica (Archangelica officinalis) in 2009. The morphology of fungal isolates was tested on standard culture media. Moreover, the sequence analysis of ITS regions was conducted. Morphological similarity of P. complanata Polish isolates to the reference isolate obtained from CBS culture collection was determined and together with the molecular analysis confirmed the affiliation of
Beata Zimowska,
Ewa Dorota Zalewska,
Ewa Dorota Król,
Agnieszka Furmańczyk
Polish Journal of Microbiology, Volume 66 , ISSUE 2, 281–285
research-article | 13-April-2020
Goldansaz, 1998; Pakniyat and Taghizadeh, 2000; Namjoo et al., 2019) with no morphological and morphometric data. However, the morphological characters and morphometric data were provided for an Iranian population of the species by Kolaei et al. (2016) from Kerman. The genera Aprutides and Schistonchus were not characterized from Iran with molecular data, unlike the other previously mentioned genera. Based on a recovered population of S. caprifici from syconia of Ficus carica collected from a fig garden
Hadi Karimipour Fard,
Hamid Zare
Journal of Nematology, Volume 52 , 1–3
research-article | 30-November-2019
overlapping morphometrics of some species groups in the genus Xiphinema make quarantine regulations and protection methods more difficult. Therefore, accurate identification of Xiphinema species using integrate approach is strongly recommended to create a basis for plant pest management.
In Vietnam, eight species of the genus Xiphinema have been reported, however, molecular identification are not available for most of them (Nguyen and Nguyen, 2000), and thus, a higher diversity of Xiphinema spp. is
Huu Tien Nguyen,
Thi Duyen Nguyen,
Thi Mai Linh Le,
Quang Phap Trinh
Journal of Nematology, Volume 52 , 1–4
research-article | 17-March-2020
report of the alfalfa cyst nematode in North America was reported in Kansas by Powers et al. (2019). Previously, unpublished records of this cyst species reproducing on alfalfa indicated the presence of this nematode in the Great Plains states of Kansas and Montana (Powers et al., 2019).
The soil sample was collected from Delta, Utah (Millard county) and sent to University of Idaho for initial evaluation, after which it was submitted to USDA-ARS in Beltsville, MD for morphological and molecular
Zafar A. Handoo,
Andrea M. Skantar,
Saad L. Hafez,
Mihail R. Kantor,
Maria N. Hult,
Stephen A. Rogers
Journal of Nematology, Volume 52 , 1–4
research-article | 30-November-2018
Province, southwestern Iran. Voucher specimens including 12 females were deposited in the collection of the Department of Plant Protection, College of Agriculture, University of Zanjan, Zanjan, Iran.
Molecular phylogenetic status
The amplification of D2-D3 expansion fragments of 28S rRNA gene sequences of C. paramaritus n. sp. and C. cancellatus yielded a single fragment of 736 (after editing 635) and 751 (after editing 642) bp, respectively. The D2-D3 expansion fragment of 28S rRNA alignment
Manouchehr Hosseinvand,
Ali Eskandari,
Reza Ghaderi
journal of nematology, Volume 51 , 1–10
research-article | 30-November-2019
has two entomoparasitic and free-living mycetophagous generations (Siddiqi, 2000). The list of the valid species under the genus is given by several authors (Siddiqi, 2000; Miraeiz et al., 2017; Yu et al., 2017; Morris et al., 2018). Almost all old species under the genus have been established based upon traditional approaches. On the contrary, several recently described species include molecular data (e.g., Yu et al., 2009, 2011, 2013, 2014; Morris et al., 2013; Tomar et al., 2015; Kanzaki et al
Fariba Heydari,
Joaquín Abolafia,
Majid Pedram
Journal of Nematology, Volume 52 , 1–13
research-article | 30-November-2020
county, Colorado, USA. A, B: J2 anterior ends; C, D: J2 tails; E: J2 lateral field; F: Cyst posterior (ventral view); G: Cyst cone mount.
Representative J2 from two CO isolates and three MN isolates were used for molecular confirmation of the species, using two ribosomal genes (internal transcribed spacer, ITS 1 and 2 and large ribosomal subunit, 28S), one nuclear gene (partial heat shock protein 90, Hsp90), and one mitochondrial gene (partial cytochrome oxidase I, COI). Markers were amplified
Andrea M. Skantar,
Zafar A. Handoo,
Mihail R. Kantor,
Saad L. Hafez,
Maria N. Hult,
Kathryn Kromroy,
Kimberly Sigurdson,
Michelle Grabowski
Journal of Nematology, Volume 53 , 1–7
Original Paper | 15-March-2016
Carotenoids are known to possess immense nutraceutical properties and microorganisms are continuously being explored as natural source for production of carotenoids. In this study, pigmented bacteria belonging to Flavobacteriaceae family were isolated using kanamycin-containing marine agar and identified using the molecular techniques and their phenotypic characteristics were studied along with their potential to produce carotenoids. Analysis of random amplification of polymorphic DNA (RAPD
Rama Sowmya,
Nakkarike M. Sachindra
Polish Journal of Microbiology, Volume 65 , ISSUE 1, 77–88
research-article | 06-November-2020
). From the Russian species E. rivalis, it differs in being longer (2.16-2.46 vs 1.46-2.29 mm, n = 15), the lip region is less differentiated (weak vs deep constriction), and it has a longer odontostyle (32-35 vs 28-30 µm). Males of E. rivalis are as abundant as females, whereas they are unknown in E. procerus n. sp. and thus likely to be rare or absent.
Evolutionary relationships, as derived from the analysis of D2-D3 28S-rRNA gene sequences, are presented in a molecular tree (Fig. 3). The new
Thi Anh Duong Nguyen,
Reyes Peña-Santiago
Journal of Nematology, Volume 52 , 1–8
research-article | 30-November-2019
glycerin using De Grisse’s (1969) method. The characteristics perineal patterns of the second-stage juvenile (J2) were used to identify the test Meloidogyne species (Perry et al., 2009).
Figure 1:
Meloidogyne hapla Chitwood, 1949. (A) Roots of kiwi tree affected. (B) Mature female on the root.
The molecular characterization followed the methods described in Álvarez-Ortega et al. (2019). The ribosomal ITS and LSU sequences were analyzed and aligned using the program BioEdit (Hall, 1999), aligned
Ebrahim Shokoohi,
Phatu W. Mashela
Journal of Nematology, Volume 52 , 1–5
original-paper | 22-October-2019
concluded that the overall HBsAg positivity rate for the TRNC is 1.2% (Arikan et al. 2016).
There is a high demand for genotype information and investigation regarding HBV infected individuals. This importance rules to be informed regarding molecular and epidemiological specifications. As with the aim of this importance, we have aimed to analyze the dispersion of genotype/subgenotype/serotype together with pol gene mutations, which are related to NA therapy, and S gene mutations.
Experimental
UNAL SUMER,
MURAT SAYAN
Polish Journal of Microbiology, Volume 68 , ISSUE 4, 449–456
research-article | 30-November-2019
removed and, as wet gas, enters condenser (5). In this place gas is cooled down and water vapor condenses. Liquid is taken out in water knock out (6). After that gas is compressed in a compressor (7) to compensate losses in the unit and equalize pressure with raw gas. At the end it is reinjected to the stream coming from well [15].
Figure 3.
Scheme of adsorption drying process (source: [15])
The most commonly used adsorbents in gas drying processes are silica gel (SiO2), molecular sieves (zeolites
Natalia GENEROWICZ
Architecture, Civil Engineering, Environment, Volume 13 , ISSUE 3, 73–83
Article | 21-July-2017
Enchodorus yeatsi n. sp., a new species of the rare genus Enchodorus is described and illustrated based on its morphological and molecular characters. It was recovered from southern Iran. Females of the new species are characterized by having 1,511.3- to 1,792.5-mm long slender body, angular lip region having prominent papillae, 12- to 13-mm long odontostyle, double guiding ring, simple rod-like odontophore, didelphic–amphidelphic reproductive system, and 102- to 160-mm
MAJID PEDRAM
Journal of Nematology, Volume 49 , ISSUE 1, 21–26
Original Paper | 28-December-2016
The objective of the study was to perform a comparative analysis of phenotypic and genetic similarity, determination of resistance profiles, detection of toxin-encoding genes and molecular typing of Clostridium perfringens isolates originating from patients with gas gangrene. The study encompassed three patients with a clinical and microbiological diagnosis of gas gangrene who were hospitalized in one of the hospitals of the Kujawsko-Pomorskie province in the same period of time between 8th
Monika Brzychczy-Włoch,
Dorota Ochońska,
Anna Piotrowska,
Małgorzata Bulanda
Polish Journal of Microbiology, Volume 65 , ISSUE 4, 399–406
Article | 04-January-2021
production, breeding failure, and abortion in the domestic animals infected with Brucella spp. [12].
The development of DNA markers and molecular biology techniques are important tools for genomics-based studies in animal biotechnology [13]. It has led to genetic improvement and markers now help in the selection of best quality breeds of animals. Over the last 20 years, DNA markers have resulted in tremendous genetic improvement in farm animals. Recent developments in molecular biology techniques have
Sulaiman Mohammed Abu Sulayman,
Roop Singh Bora,
Jamal S.M. Sabir,
Mohamed Morsi M. Ahmed
Postępy Mikrobiologii - Advancements of Microbiology, Volume 59 , ISSUE 4, 337–344
research-article | 28-April-2020
Rafiqius to include A. saeedi and other morphological related species as A. bodenheimeri (Steiner, 1936; Thorne, 1937). This newly proposed genus was differentiated from Acrobeloides (Cobb, 1924) according to the morphology of the lip region, having seta-like processes at labial primary axils. However, the creation of this new genus was considered unjustified by De Ley et al. (1999). Unfortunately, none of these studies provided molecular study.
Figure 1:
Acrobeloides saeedi (isolate KMW) (Siddiqi
Aasha Rana,
Aashaq Hussain Bhat,
Suman Bhargava,
Ashok Kumar Chaubey,
Joaquín Abolafia
Journal of Nematology, Volume 52 , 1–21
Original Paper | 04-September-2018
To understand the molecular epidemiology and antibiotic resistance of air and clinical isolates of Acinetobacter baumannii, the intensive care unit settings of a hospital in Northern China were surveyed in 2014. Twenty non-duplicate A. baumannii isolates were obtained from patients and five isolates of airborne A. baumannii were obtained from the wards’ corridors. Pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to analyze the homology relationships of
MEIJIE JIANG,
YUNQING MU,
NING LI,
ZHIJUN ZHANG,
SHULIN HAN
Polish Journal of Microbiology, Volume 67 , ISSUE 3, 333–338
research-article | 30-November-2018
., 2004; Jones et al., 2013).
While numerous reports of Rotylenchulus associated with Musa spp. in Colombia exist (Zuñiga et al., 1979; Barriga and Cubillos, 1980; Curiel and Ospino, 2001; Gómez, 2001; Guzmán et al., 2012), detailed morphological, morphometric, and molecular data were not included. Thus, in Colombia, there is limited knowledge as to which species of Rotylenchulus are associated with Musa spp. which impedes the deployment of effective management strategies to control the species. To
Donald Riascos-Ortiz,
Ana Teresa Mosquera-Espinosa,
Francia Varón De Agudelo,
Claudio Marcelo Gonçalves de Oliveira,
Jaime Eduardo Muñoz-Flórez
Journal of Nematology, Volume 51 , 1–13
research-article | 30-November-2020
. Morphologically, they all have at least one tylenchid-like character, such as small spherical median bulb, tylenchid-type bursa, and elongate posteriorly directed stylet knobs (Mirzaie Fouladvand et al., 2019a). Phylogenetically, although molecular data are unavailable for the two genera Tylaphelenchus and Albiziaphelenchus, recent phylogenetic analysis based on rRNA small subunit (SSU) and D2-D3 expansion domains of large subunit (LSU D2-D3) confirmed that Pseudaphelenchus and Basilaphelenchus form a
Bashiy Akol,
Qiuling Huang,
Borong Lin,
Honghong Wang,
Jinling Liao,
Kan Zhuo
Journal of Nematology, Volume 53 , 1–12
Article | 21-July-2017
results once the animals entered their hosts’ roots. We have compared several extraction procedures to ascertain whether a single protocol was capable of recovering high-quality, high-molecular-weight RNA from newly hatched J2 larva as well as from larva embedded in roots of both potatoes (Solanum tuberosum L. cv. Desiree) and a very distantly related species, Solanum sisymbriifolium. Although it was possible to recover large amounts of RNA from J2 larvae using Proteinase K
N. CAROL CASAVANT,
JOSEPH C. KUHL,
FANGMING XIAO,
ALLAN B. CAPLAN,
LOUISE-MARIE DANDURAND
Journal of Nematology, Volume 49 , ISSUE 1, 12–20
Research Article | 03-September-2018
study. Molecular characterization based on 18SSU rDNA sequencing performed to confirm the taxonomic position of this species and to documents the morphological data. Sequence alignment detects a percent of identity up to 88.0% with other Heteroxynematidae species. Phylogenetic analysis showed that the present recorded is a putative sister taxon to A. tetraptera recorded in a previous study. The SSU rDNA sequence has been deposited in the GenBank under the accession no. MG019400.
Rewaida Abdel-Gaber,
Fathy Abdel-Ghaffar,
Saleh Al Quraishy,
Kareem Morsy,
Rehab Saleh,
Heinz Mehlhorn
Journal of Nematology, Volume 50 , ISSUE 2, 117–132
Review | 20-March-2020
between the glycophorin genes. Antigens in the MNS system are fully developed at birth. This review summarizes aspects of the MNS system, including the molecular basis of some antigens in the MNS blood group system. Readers are referred to existing excellent reviews for background information.1–9 Throughout this document, information given without references can be found in the reviews listed previously, and the reader is referred to these reviews for references to original reports.
Marion E. Reid
Immunohematology, Volume 25 , ISSUE 3, 95–101
research-article | 26-March-2021
parasites, and opportunities to influence the changes taking place in their ethology, pathogenesis, evolution, and divergence. A solid and clear taxonomic framework is necessary for examine the basic biology of the parasites, and establish the control system in epidemiology and medicine. Thus, the scope of this study was to accurately identify species of nematodes found in minced salmon O. nerka using the molecular genetics methods and reconstruct phylogenetic relationships within the family Anisakidae
Alina E. Safonova,
Anastasia N. Voronova,
Konstantin S. Vainutis
Journal of Nematology, Volume 53 , 1–10
Article | 21-July-2017
northwestern North America were discovered. Nematodes were identified and characterized microscopically and molecularly with 28S ribosomal RNA (rRNA) and 18S rRNA molecular markers. From P. angustifolia, Aphelenchoides saprophilus was inferred to be closest to another population of A. saprophilus among sequenced taxa in the 18S tree. From P. trichocarpa, Laimaphelenchus heidelbergi had a 28S sequence only 1 bp different from that of a Portuguese population, and 1 bp different from the
LYNN K. CARTA,
SHIGUANG LI,
ANDREA M. SKANTAR,
GEORGE NEWCOMBE
Journal of Nematology, Volume 48 , ISSUE 1, 28–33
research-article | 25-May-2020
) analysis (Powers et al., 2016). Mesocriconema nebraskense (Olson et al., 2017) was described as a monosexual, cryptic species sympatrically distributed with its cryptic counterpart, M. curvatum (Raski, 1952; Loof and De Grisse, 1989; Olson et al., 2017). In this paper, we describe the new species M. abolafiai n. sp., based on morphological and molecular characteristics.
Material and methods
Nematode populations and morphological characterization
The specimens were recovered from two localities in
Hossein Mirbabaei Karani,
Ali Eskandari,
Reza Ghaderi,
Akbar Karegar
Journal of Nematology, Volume 52 , 1–17
Research Article | 31-May-2018
opening large, and relative proximity of vulva to the anus. Detailed morphological and molecular characterization of the new genus/species is presented along with the comparison of the related genera.
Qing Yu,
Maria Munawar,
Jianfeng Gu,
Weimin Ye
Journal of Nematology, Volume 50 , ISSUE 1, 69–76
research-article | 17-March-2020
., 2016). Thus, additional identification methods, particularly molecular-based identification, which requires a small amount of nucleic acid extracted from a single individual, will allow more accurate nematode identification, regardless of developmental stage (Handoo et al., 2016). The present study aimed to characterize R. similis using a combined morphological and morphometric approach and to confirm its identity using rDNA sequencing.
Materials and methods
Nematode populations
We collected 314
Doreen M. Mgonja,
Gladness E. Temu,
Joseph C. Ndunguru,
Magreth F. Mziray,
Sylvester L. Lyantagaye,
Nessie D. Luambano
Journal of Nematology, Volume 52 , 1–8
Original Paper | 26-August-2016
using microarrays technology confirmed high effectiveness of this method in determining STEC virulotypes which provides data suitable for molecular risk assessment of the potential virulence of this bacteria.
Aleksandra Januszkiewicz,
Waldemar Rastawicki
Polish Journal of Microbiology, Volume 65 , ISSUE 3, 261–269
research-article | 30-November-2020
morphological features only (Nguyen and Nguyen, 2000), except for the recent report of M. javanica on black pepper (Piper nigrum L.) applied specific primers in identifying this species (Nguyen et al., 2020).
In the past, Meloidogyne species were identified using morphological and morphometric characterizations, and consequently, a large number of Meloidogyne spp. were described without molecular data (Karssen, 2002; Perry et al., 2009). Although molecular approach has facilitated the identification process
Ke Long Phan,
Thi Mai Linh LE,
Huu Tien Nguyen,
Thi Duyen Nguyen,
Quang Phap Trinh
Journal of Nematology, Volume 53 , 1–8
Article | 01-April-2020
resolve blood donor typing problems caused by B(A) alleles,a serologic and molecular study of nine unrelated Chinese individuals and three families carrying B(A) alleles was conducted. Allele B(A)02 with a 700C>G mutation,allele B(A)04 with a single 640A>G substitution, and allele B(A)05 with a 641T>C mutation were detected in multigenerational families and unrelated blood donors. Neither the B(A)01 nor B(A)03 alleles with 703A>G substitutions were observed in this study. In addition, a
Zhong-Hui Guo,
Dong Xiang,
Zi-Yan Zhu,
Xi Liu,
He-Ping Chen,
Jian-Lian Wang,
Da-Zhuang Liu,
Tong-Mao Zhao
Immunohematology, Volume 23 , ISSUE 2, 69–74
Research Article | 03-September-2018
molecular analysis of many D. weischeri specimens from Canada is presented. Individuals from 41C. arvense or yellow pea grain samples with seeds of C. arvense from the Prairie Provinces were sequenced for the internal transcribed spacer (ITS rDNA), large subunit (LSU) D2D3 28S rDNA, partial segment of small subunit (SSU) 18S rDNA, and the heat shock protein Hsp90 gene. The analysis also included D. weischeri individuals from C. arvense from Russia and garlic with D. dipsaci from the Provinces of Ontario
Mehrdad Madani,
Mario Tenuta
Journal of Nematology, Volume 50 , ISSUE 2, 163–182
research-article | 30-November-2019
, before DNA extraction (see Table 1).
Molecular characterization
Following morphological identification, the same individual nematodes were picked from temporary slides and used for extraction of genomic DNA using a quick alkaline lysis protocol (Janssen et al., 2016). DNA was amplified by preparing 24 μl PCR master mix comprising 16 μl double sterilized distilled water, 2.5 μl 10x buffers, 2 μl MgCl2, 0.05 μl of dNTP (10 mM), 1 μl of reverse and forward primers, 0.05 μl of Toptaq and 2 μl of
Yao A. Kolombia,
Oluwadamilola Ogundero,
Emmanuel Olajide,
Nicole Viaene,
P. Lava Kumar,
Danny L. Coyne,
Wim Bert
Journal of Nematology, Volume 52 , 1–25
Case report | 25-March-2020
primers designed to amplify RHD exons 3, 4, or 7. Two assays that detect the hybrid Rhesus box showed deletion of RHD. Amplification of RHCE in the patient’s DNA was as efficient as that of control samples, and multiplex and PCR-RFLP assays predicted her RBCs would be C–E–c+e+. Based on finding a hybrid Rhesus box and absence of D-specific exons, we conclude that DNA from the patient’s WBCs carries a deleted RHD. This explains the molecular mechanism
Ann Murdock,
Deborah Assip,
Kim Hue-Roye,
Christine Lomas-Francis,
Zong Hu,
Sunitha Vege,
Connie M. Westhoff,
Marion E. Reid
Immunohematology, Volume 24 , ISSUE 4, 160–164
research-article | 16-April-2019
the new species, according to the polytomous key of Loof and Luc (1990), are: A4, B3, C5a, D6, E5, F4, G3, H2, I3, J4, K2, L1.
Based on the molecular and morphological similarities, the new species is closely related to X. aceri (Chizhov et al., 1986); X. granatum (Pedram, Pourjam, Palomares-Rius, Ghaemi, Cantalapiedra-Navarrete and Castillo, 2012); X. herakliense (Tzortzakakis et al., 2015); X. zagrosense (Ghaemi et al., 2012), and X. vuittenezi (Luc et al., 1964), but it can be separated using
Nasir Vazifeh,
Gholamreza Niknam,
Habibeh Jabbari,
Arezoo Naghavi
Journal of Nematology, Volume 51 , 1–17
research-article | 30-November-2020
glands opening.
To date, among predaceous nematodes of the order Mononchida in Vietnam, 17 Iotonchus species have been recorded from several provinces (Vu, 2015). In the present study, Iotonchus lotilabiatus n. sp. is described based on morphological, morphometric, and molecular data from the Bat Xat Natural Reserve in Lao Cai Province, Vietnam. An updated key to species based on female characteristics and a compendium of all the known species are also provided.
Material and methods
Nematode
Tam T. T. Vu,
Thi Mai Linh Le,
Thi Duyen Nguyen
Journal of Nematology, Volume 53 , 1–22