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original-paper | 31-October-2019

Molecular Identification of Vibrio alginolyticus Causing Vibriosis in Shrimp and Its Herbal Remedy

the shrimp farms. Sample No. Symptoms Site of isolation 01–04 Deformed and yellowish colored hepatopancreas Hepatopancreas 05–06 Blackish colored hepatopancreas Hepatopancreas 07–11 Discolored hepatopancreas Hepatopancreas 12–13 Yellowish colored hepatopancreas Hepatopancreas 14–15 No visible symptom Hepatopancreas Molecular identification of Vibrio isolates. Of a total of 20 isolates, genomic DNA of four representatives of Vibrio isolates (2A1a, 2A3, 2A11 and 2V21) was


Polish Journal of Microbiology, Volume 68 , ISSUE 4, 429–438

Research Article | 03-September-2018

Molecular Identification of Entomopathogenic Nematode Isolates from the Philippines and their Biological Control Potential Against Lepidopteran Pests of Corn

most susceptible to H. indica PBCB (21.90 hr) and S. abbasi (21.18 hr). Our results suggest that H. indica PBCB and S. abbasi MBLB are good candidates as biological control agents against these insect pests of corn. Moreover, O. furnacalis as alternative bait for EPN was discussed. To date, this is the most extensive research on Philippine EPN, comprised of wide sampling coverage, molecular identification and bioefficacy assays.

Barbara L. Caoili, Romnick A. Latina, Regina Faye C. Sandoval, Joey I. Orajay

Journal of Nematology, Volume 50 , ISSUE 2, 99–110

Research Article | 03-December-2018

First report of Pratylenchus vulnus associated with apple in Tunisia

Noura Chihani-Hammas, Lobna Hajji-Hedfi, Hajer Regaieg, Asma Larayedh, Ahmed Badiss, Yu Qing, Horrigue-Raouani Najet

Journal of Nematology, Volume 50 , ISSUE 4, 579–586

Research Article | 03-December-2018

First report of Bursaphelenchus antoniae from Pinus strobus in the U.S.

Lynn K. Carta, R. L. Wick

Journal of Nematology, Volume 50 , ISSUE 4, 473–478

research-article | 24-April-2020

Cephalenchus driekieae n. sp. (Nematoda: Tylenchidae) from South Africa, a new member of the genus with a long pharyngeal overlap

Milad Rashidifard, Gerhard Du Preez, Joaquín Abolafia, Majid Pedram

Journal of Nematology, Volume 52 , 1–10

research-article | 30-November-2020

First report of morphological and molecular characterization of Moroccan populations of Globodera pallida

A. Hajjaji, R. Ait Mhand, N. Rhallabi, F. Mellouki

Journal of Nematology, Volume 53 , 1–8

research-article | 30-November-2019

First report of Xiphinema hunaniense Wang & Wu, 1992 (Nematoda: Longidoridae) in Vietnam

overlapping morphometrics of some species groups in the genus Xiphinema make quarantine regulations and protection methods more difficult. Therefore, accurate identification of Xiphinema species using integrate approach is strongly recommended to create a basis for plant pest management. In Vietnam, eight species of the genus Xiphinema have been reported, however, molecular identification are not available for most of them (Nguyen and Nguyen, 2000), and thus, a higher diversity of Xiphinema spp. is

Huu Tien Nguyen, Thi Duyen Nguyen, Thi Mai Linh Le, Quang Phap Trinh

Journal of Nematology, Volume 52 , 1–4

research-article | 30-November-2019

Morphological and molecular characterization of Hoplolaimus pararobustus (Schuurmans Stekhoven and Teunissen, 1938) Sher 1963 with its first report on Zea mays roots in Namibia

corms and roots of banana where they were likely to cause damage to the crop (Sikora et al., 2018). Phylogenetic analysis of Hoplolaimus spp. using D2–D3 expansion of 28 S and internal transcribed spacer (ITS1) ribosomal DNA sequences resolved the phylogeny of the genus and were useful in molecular identification of Hoplolaimus spp. (Bae et al., 2008). In addition, the PCR-RFLP method was applied by different researchers to evaluate the genetic diversity of Hoplolaimus spp. (Robbins et al., 2009

Mariette Marais, Esther van den Berg, Hendrika Fourie, Milad Rashidifard

Journal of Nematology, Volume 52 , 1–12

Article | 03-December-2017

Morphological and Molecular Identification of Longidorus euonymus and Helicotylenchus multicinctus from the Rhizosphere of Grapevine and Banana in Greece


Journal of Nematology, Volume 49 , ISSUE 3, 233–235

Short Communication | 09-March-2018

The Prevalence of Campylobacter spp. in Polish Poultry Meat

The prevalence, count and molecular identification of Campylobacter spp. in Polish poultry meat were analysed. 181 samples of meat from chicken (70), turkey (47), duck (54) and goose (10) were studied. Campylobacter spp. was found in 64% of meat samples. The highest prevalence of this pathogen was detected for duck meat. On average 80% of duck samples were contaminated with Campylobacter spp. The counts of Campylobacter spp. in positive samples remained under ten colony forming units per gram

Anna Szosland-Fałtyn, Beata Bartodziejska, Joanna Królasik, Beata Paziak-Domańska, Dorota Korsak, Magdalena Chmiela

Polish Journal of Microbiology, Volume 67 , ISSUE 1, 117–120

research-article | 17-March-2020

Molecular and morphological characterization of the alfalfa cyst nematode, Heterodera medicaginis, from Utah

report of the alfalfa cyst nematode in North America was reported in Kansas by Powers et al. (2019). Previously, unpublished records of this cyst species reproducing on alfalfa indicated the presence of this nematode in the Great Plains states of Kansas and Montana (Powers et al., 2019). The soil sample was collected from Delta, Utah (Millard county) and sent to University of Idaho for initial evaluation, after which it was submitted to USDA-ARS in Beltsville, MD for morphological and molecular

Zafar A. Handoo, Andrea M. Skantar, Saad L. Hafez, Mihail R. Kantor, Maria N. Hult, Stephen A. Rogers

Journal of Nematology, Volume 52 , 1–4

research-article | 30-November-2019

Molecular identification of Bursaphelenchus cocophilus associated to oil palm (Elaeis guineensis) crops in Tibu (North Santander, Colombia)

Siddiqi (1975), Mai and Lyon (1975), Gerber et al. (1989). The morphometric data were taken using a compound microscope ZEISS Axio (China, microscope reference: 3136006171 Axio.A1). Molecular identification DNA extraction was performed by the proteinase K method (Riascos-Ortiz et al., 2019). A specimen was divided into three parts with a sterile scalpel, transferring the sections to Eppendorf tubes with 15 µL lysis buffer (50 mM KCl, 10 mM Tris pH 8.0, 15 mM MgCl2, 0.5% Triton x – 100, 4.5% Tween

Greicy Andrea Sarria, Donald Riascos-Ortiz, Hector Camilo Medina, Yuri Mestizo, Gerardo Lizarazo, Francia Varón De Agudelo

Journal of Nematology, Volume 52 , 1–12

original-paper | 05-August-2020

Molecular Identification and Prevalence of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in Erbil City, Northern Iraq


Polish Journal of Microbiology, Volume 69 , ISSUE 3, 263–272

research-article | 19-February-2021

First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water

Hoang Van Hien, Bui Thi Dung, Ha Duy Ngo, Pham Ngoc Doanh

Journal of Nematology, Volume 53 , 1–9

research-article | 26-March-2021

First report on molecular identification of Anisakis simplex in Oncorhynchus nerka from the fish market, with taxonomical issues within Anisakidae

Alina E. Safonova, Anastasia N. Voronova, Konstantin S. Vainutis

Journal of Nematology, Volume 53 , 1–10

research-article | 30-November-2020

Pathogen identification of Gentiana macrophylla root-knot nematode disease in Yulong, China

Wentao Wu, Shanshan Xu, Zewen Gao, Shusheng Zhu, Youyong Zhu, Yang Wang, Xiahong He

Journal of Nematology, Volume 53 , 1–3

Research Article | 03-September-2018

First Report of Carrot Cyst Nematode Heterodera carotae in Mexico: Morphological, Molecular Characterization, and Host Range Study

was made using light and scanning electron microscopy of the second stage juveniles, females, males and cysts, and the host range study, was performed with nine different plants from five families. The molecular identification was made by sequencing and analysing the ITS rRNA and partial COI genes. It was shown that using presently available molecular tools it is not possible to make an accurate differentiation of H. carotae from H. cruciferae. The host range test allowed to distinguish these

Ilia Mariana Escobar-Avila, Edgar Óliver López-Villegas, Sergei A. Subbotin, Alejandro Tovar-Soto

Journal of Nematology, Volume 50 , ISSUE 2, 229–242

research-article | 30-November-2018

Impact of a conservation agriculture system on soil characteristics, rice yield, and root-parasitic nematodes in a Cambodian lowland rice field

-parasitic nematodes In conjunction with the molecular identification of the RPN species, the population dynamics of the RPN were studied using the RPN population densities at four rice development stages: seedling, tillering, milky and harvest. Eggs extracted from infected roots were hatched under controlled conditions (Bellafiore et al., 2015). For the tillering stage in 2014 and the harvest stage in both 2014 and 2015, the hatching was compromised and we were not able to collect J2. The average number

Malyna Suong, Elodie Chapuis, Vira Leng, Florent Tivet, Dirk De Waele, Huế Nguyễn Thị, Stéphane Bellafiore

Journal of Nematology, Volume 51 , 1–15

research-article | 30-March-2020

Characterization of Vittatidera zeaphila (Nematoda: Heteroderidae) from Indiana with molecular phylogenetic analysis of the genus

recovered. Soil samples were submitted to one of us (JF), who extracted the nematode cysts and sent them to the USDA-ARS, Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL), Beltsville, MD for morphological and molecular identification. Cysts and second-stage juveniles (J2) conformed morphologically and morphometrically to V. zeaphila. We report here the first occurrence of this species in Indiana, thus representing the third state after Tennessee, and Kentucky in the United

Andrea M. Skantar, Zafar A. Handoo, Mihail R. Kantor, Lynn K. Carta, Jamal Faghihi, Virginia Ferris

Journal of Nematology, Volume 52 , 1–8

research-article | 30-November-2020

Description of Longidorella (Saevadorella) caspica n. sp. (Dorylaimida: Nordiidae) from north Iran

Fariba Heydari, Mohammad Reza Atighi, Ebrahim Pourjam, Majid Pedram

Journal of Nematology, Volume 53 , 1–11

original-paper | 05-December-2019

Diversity, Virulence Factors, and Antifungal Susceptibility Patterns of Pathogenic and Opportunistic Yeast Species in Rock Pigeon (Columba livia) Fecal Droppings in Western Saudi Arabia

HIV patients (Cogliati 2013; Hagen et al. 2015; Rajasingham et al. 2017; Esher et al. 2018; Magalhães Pinto et al. 2019). Molecular identification tools such as real-time PCR, multiplex PCR, and RFLP PCR have been successful in detecting and identifying various yeast species in clinical and environmental samples. Furthermore, the identification of yeast-based on fungal ribosomal DNA (rDNA) has become popular as an accurate molecular tool. This tool allows for the detection of 18S and 26S subunits


Polish Journal of Microbiology, Volume 68 , ISSUE 4, 493–504

research-article | 30-November-2019

Oscheius myriophila (Nematoda: Rhabditida) isolated in sugar cane soils in Mexico with potential to be used as entomopathogenic nematode

obtain single colonies. The bacteria were identified by biochemical methods using Gram staining, motility tests, and assays of protease activity and lecithinase and antibiotic production as suggested by Boemare et al. (1997) and Akhurst (1980). For molecular identification of the isolated bacteria, the cells were incubated in LB liquid medium for 12 h at 30 °C, and DNA extraction was then carried out with the Easy-DNA™ kit following the instructions recommended by the manufacturer. Then

Iveth del Rocio Castro-Ortega, Juan Manuel Caspeta-Mandujano, Ramón Suárez-Rodríguez, Guadalupe Peña-Chora, José Augusto Ramírez-Trujillo, Karina Cruz-Pérez, Iván Arenas Sosa, Víctor Manuel Hernández–Velázquez

Journal of Nematology, Volume 52 , 1–8

Research Article | 17-October-2018

First Report of Bitylenchus hispaniensis, Pratylenchoides alkani, and Helicotylenchus vulgaris in Association with Cultivated and Wild Olives in Crete, Greece and Molecular Identification of Helicotylenchus microlobus and Merlinius brevidens

Emmanuel A. Tzortzakakis, Carolina Cantalapiedra-Navarrete, Maria Kormpi, Maria S. Lazanaki, Pablo Castillo, Antonio Archidona-Yuste

Journal of Nematology, Volume 50 , ISSUE 3, 413–418

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