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Short Communication | 09-March-2018

Rapid Detection of Bloodstream Pathogens in Oncologic Patients with a FilmArray Multiplex PCR Assay: a Comparison with Culture Methods

The results of the FilmArray® Blood Culture Identification Panel (BCID) (BioFire Diagnostics) and the culture with susceptibility testing of 70 positive blood cultures from oncologic patients were compared. The multiplex PCR assay (BCID) identified 81 of the 83 isolates (97.6%), covered by the panel. The panel produced results in significantly shorter time than standard identification methods, when counted from receiving positive blood cultures bottles to the final results. It is an accurate

Maria Szymankiewicz, Beata Nakonowska

Polish Journal of Microbiology, Volume 67 , ISSUE 1, 103–107

research-article | 30-November-2020

First report and new molecular and morphological characterizations of root-knot nematode, Meloidogyne javanica, infecting ginger and long coriander in Vietnam

slides following Nguyen et al. (2019). For morphological characterization, measurements and pictures were taken from permanent slides using Carl Zeiss Axio Lab. A1 light microscope equipped with a Zeiss Axiocam ERc5s digital camera. For molecular characterization, Multiplex-PCR using primers Mi2F4/Mi2R1, Far/Rar, and Fjav/Rjav was performed following Kiewnick et al. (2013) to quickly identify M. javanica from closely related species in the tropical root-knot nematode group. The D2-D3 region of 28S

Ke Long Phan, Thi Mai Linh LE, Huu Tien Nguyen, Thi Duyen Nguyen, Quang Phap Trinh

Journal of Nematology, Volume 53 , 1–8

research-article | 30-November-2020

First report of morphological and molecular characterization of Moroccan populations of Globodera pallida

at maximum frequency and incubated at 65°C for 1 hr and then at 95°C for 10 min after removal of the beads (Subbotin et al., 1999). The total genomic DNA suspension was centrifuged at 13,000 rpm for 1 min and was quantified and its purity assessed using Thermo Scientific™ NanoDrop spectrophotometers and then stored at −20°C for later use. PCR amplification The multiplex PCR mixture consisted of 25 μl which contained: Taq DNA polymerase buffer 1X, primers 0.64 µM, MgCl2: 2 mM, dNTPs: 0.25 mM

A. Hajjaji, R. Ait Mhand, N. Rhallabi, F. Mellouki

Journal of Nematology, Volume 53 , 1–8

Article | 26-October-2019

An overview of the use of SNaPshot for predicting blood group antigens

Flavia R.M. Latini, Lilian M. Castilho

Immunohematology, Volume 31 , ISSUE 2, 53–57

Report | 25-March-2020

Principles of PCR-based assays

DNA-based assays are powerful tools to predict the blood group of an individual and are rapidly gaining in popularity.  DNA, which can be extracted from various sources using commercial kits, is amplified by PCR to obtain a sufficient amount of the target of interest for analysis.  There are different types of PCR assays: standard single PCR (followed by RFLP or sequencing), allele-specific PCR, multiplex PCR, and real-time PCR.  Microarray platforms are a newer application of

Kim Hue-Roye, Sunitha Vege

Immunohematology, Volume 24 , ISSUE 4, 170–175

research-article | 30-November-2019

First report of the stubby-root nematode Nanidorus minor infecting Paspalum vaginatum, seashore paspalum grass in Georgia, USA

complimentary sequences were assembled into a consensus sequence. Upon nucleotide blast on the National Center for Biotechnology Information (ncbi.nlm.nih.gov) website, they were all confirmed to be N. minor. The top pairwise identity with N. minor references was 100% for 360F/932R (accession no. MT36688) and BL18/5818 (MT372094), and 99.73% for D2A/D3B (MT371258). In contrast, the highest identity to other members of the Nanidorus sp. was in the range of 96% or lower. A one-step multiplex PCR method was

Ganpati B. Jagdale, Fereidoun Forghani, Katherine Martin, Abolfazl Hajihassani, Alfredo Dick Martinez-Espinoza

Journal of Nematology, Volume 52 , 1–3

Report | 26-October-2019

High-resolution melting analysis as an alternative method for human neutrophil antigen genotyping

Human neutrophil antigen (HNA)-typed granulocyte panels are widely used to screen for the presence of HNA antibodies and to determine antibody specificity. Many laboratories screen donors for HNA genotypes using low-throughput methods such as allele-specific polymerase chain reaction (PCR), PCR–restriction fragment–length polymorphism, and multiplex PCR. In the present study, we used a high-resolution melting (HRM) analysis to determine HNA genotypes. For the HRM analysis, purified

Kazuta Yasui, Mitsunobu Tanaka, Tomoya Hayashi, Nobuki Matsuyama, Ayumu Kuroishi, Rika. A. Furuta, Yoshihiko Tani, Fumiya Hirayama

Immunohematology, Volume 31 , ISSUE 1, 7–13

Original Paper | 28-December-2016

Gas Gangrene of Different Origin Associated with Clostridium perfringens Type A in Three Patients Simultaneously Hospitalized in a Single Department of Orthopedics and Traumatology in Poland

April 2015 and 20th April 2015. The three C. perfrin­gens isolates studied had identical biochemical profiles. Two isolates had identical resistance patterns, while the third presented a different profile. Using the multiplex PCR method, all isolates showed the presence of cpa gene encoding α-toxin; furthermore, the presence of the cpb2 gene encoding β2-toxin was confirmed in two isolates. Genotyping with the use of pulsed field gel electrophoresis (PFGE) indicated that the isolates

Monika Brzychczy-Włoch, Dorota Ochońska, Anna Piotrowska, Małgorzata Bulanda

Polish Journal of Microbiology, Volume 65 , ISSUE 4, 399–406

original-paper | 03-September-2019

Evidence for Infections by the Same Strain of Beta 2-toxigenic Clostridium perfringens Type A Acquired in One Hospital Ward

protocol. The presence of the genes encoding toxins of C. perfringens was confirmed using multiplex PCR amplification according to van (Asten et al. 2009) with specific primers (Genomed). The following fragments of the genes were detected (the gene product, length of the fragment): cpa (α-toxin, 324 bp); cpb (β-toxin, 195 bp); cpb2 (β2-toxin, 548 bp); etx (ε-toxin, 376 bp); iap (ζ-toxin, 272 bp); cpe (enterotoxin, 485 bp). The final images from electrophoresis were processed using QuantityOne software

DOMINIKA SALAMON, DOROTA OCHOŃSKA, ILONA WOJAK, EWA MIKOŁAJCZYK, MAŁGORZATA BULANDA, MONIKA BRZYCHCZY-WŁOCH

Polish Journal of Microbiology, Volume 68 , ISSUE 3, 323–329

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