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  • Immunohematology


Case report | 01-December-2019

Molecular RH blood group typing of serologically D–/CE+ donors: the use of a polymerase chain reaction–sequence-specific primer test kit with pooled samples

affiliated with the Transfusion Department of Udine (Northern Italy) led to the use of molecular genetic RH blood group typing with PCR-SSP test kits and DNA samples mixed in pools. From a population of 35,000 blood donors screened for D antigen by serologic typing, a total of 235 samples, distributed in pools of 5 DNA samples, were investigated. Positive results were reevaluated by opening the pools and retesting single samples. Validation of DNA-pool typing with commercial kits was done. Among 235

Donatella Londero, Mauro Fiorino, Valeria Miotti, Vincenzo de Angelis

Immunohematology, Volume 27 , ISSUE 1, 25–28

Article | 18-October-2020

The Rh blood group system: the first 60 years of discovery

Christine Lomas-Francis, Marion E. Reid

Immunohematology, Volume 16 , ISSUE 1, 7–17

Report | 12-March-2020

RHCE*ceAR encodes a partial c (RH4) antigen

The Rh blood group system is highly complex both in the number of discrete antigens and in the existence of partial antigens, especially D and e.  Recently, several partial c antigens have been reported. Here we report findings on an African American man with sickle cell disease whose RBCs typed C+c+ and whose plasma contained anti-c. Hemagglutination tests, DNA extraction, PCR-RFLP, reticulocyte RNA isolation, RT-PCR cDNA analyses, cloning, and sequencing were performed by standard

Marion E. Reid, Christine Halter Hipsky, Christine Lomas-Francis, Akiko Fuchisawa

Immunohematology, Volume 26 , ISSUE 2, 57–59

Review | 28-April-2020

Review: the Rh blood group system:an historical calendar

Peter D. Issitt

Immunohematology, Volume 21 , ISSUE 4, 141–145

Editorial | 30-November-2020

EDITORIAL: The Rh blood group system: additional complexities

Peter D. Issitt

Immunohematology, Volume 10 , ISSUE 4, 109–116

Review | 01-May-2020

Review: the molecular basis of the Rh blood group phenotypes

Franz F. Wagner, Willy A. Flegel

Immunohematology, Volume 20 , ISSUE 1, 23–36

Review | 01-May-2020

Review: the Rh blood group D antigen ...dominant, diverse, and difficult

Connie M. Westhoff

Immunohematology, Volume 21 , ISSUE 4, 155–163

Article | 01-April-2020

A confusion in antibody identification:anti-D production after anti-hrB

Christine Lomas-Francis, Rosyln Yomtovian, Claire McGrath, Phyllis S. Walker, Marion E. Reid

Immunohematology, Volume 23 , ISSUE 4, 158–160

Article | 20-April-2020

Rh antigens and phenotype frequencies of the Ibibio, Efik, and Ibo ethnic nationalities in Calabar, Nigeria

This report forms part of the study on the Rh phenotypes within the various ethnic nationalities in the south-south region of Nigeria. The aim is to demonstrate the Rh polymorphisms among the people of African descent. The frequencies of Rh blood group antigens and phenotypes of the Ibibio, Efik, and Ibo ethnic nationalities in Calabar municipality, Nigeria, were determined using standard serologic techniques. Of the 720 Calabar individuals tested, the frequencies of the Rh antigens within the

Z. Awortu Jeremiah, Chris Odumody

Immunohematology, Volume 21 , ISSUE 1, 21–24

Case report | 09-October-2019

Two cases of the variant RHD*DAU5 allele associated with maternal alloanti-D  

Jennifer A. Duncan, Susan Nahirniak, Rodrigo Onell, Gwen Clarke

Immunohematology, Volume 33 , ISSUE 2, 60–63

Article | 15-February-2021

Albumin-indirect antiglobulin test

J.R. Hamilton

Immunohematology, Volume 35 , ISSUE 2, 63–64

Article | 14-October-2020

Confirmation that the JAHK antigen is associated with the rG haplotype

Joanne Kosanke, Jill Storry, Marion Reid

Immunohematology, Volume 18 , ISSUE 2, 46–47

case-report | 25-June-2021

Severe perinatal hemolytic disease due to anti-e

G. Soler-Noda, Y. Romero-Díaz, L. Orbeal-Aldama, S. Aquino-Rojas

Immunohematology, Volume 37 , ISSUE 2, 72–77

Case report | 01-December-2019

A case of autoimmune hemolytic anemia with anti-D specificity in a 1-year-old child

Although antibodies to antigens in the Rh blood group system are common causes of warm autoimmune hemolytic anemia, specificity for only the D antigen is rare in autoimmune hemolysis in pediatric patients. This case reports an anti-D associated with severe hemolytic anemia (Hb = 2.1 g/dL) in a previously healthy 14-month-old child who presented with a 3-day history of low-grade fevers and vomiting. Because of his severe anemia, on admission to the hospital he was found to have altered mental

Rachel S. Bercovitz, Margaret Macy, Daniel R. Ambruso

Immunohematology, Volume 29 , ISSUE 1, 15–18

Article | 03-November-2020

Use of LOR-15C9 monoclonal anti-D to differentiate erythrocytes with the partial DVI antigen from those with other partial D antigens or weak D antigens

Marion E. Reid, Gregory R. Halverson, Francis Roubinet, P.A. Apoil, Antoine Blancher

Immunohematology, Volume 14 , ISSUE 3, 89–93

Review | 20-March-2020

MNS blood group system: a review

The MNS blood group system is second only to the Rh blood group system in its complexity. Many alloantibodies to antigens in the MNS system are not generally clinically significant although antibodies to low-prevalence and high-prevalence MNS antigens have caused hemolytic disease of the fetus and newborn. The MNS antigens are carried on glycophorin A (GPA), glycophorin B (GPB), or hybrids thereof, which arise from single-nucleotide substitution, unequal crossing over, or gene conversion

Marion E. Reid

Immunohematology, Volume 25 , ISSUE 3, 95–101

Article | 14-October-2020

An algorithm to locate hrB – donors for individuals with sickle cell disease

Many African Americans with sickle cell disease (SCD) develop alloantibodies to antigens in the Rh blood group system.Others have shown that from D– individuals, those lacking the high-incidence hrB antigen (> 98% prevalence) may be found among r'r African Americans. We describe an algorithm to locate units for African Americans with SCD and anti-hrB and -D. From 46,539 donations, 5136 listed African American as race. Our primary reference laboratory performed Rh phenotyping (D, C

Richard R. Gammon, Norberto D. Velasquez Jr.

Immunohematology, Volume 18 , ISSUE 3, 82–84

Case report | 14-October-2020

Moderate hemolytic disease of the newborn (HDN) due to anti-Rh17 produced by a black female with an e variant phenotype

The Rh blood group antigen e is of high incidence and has many epitopes. Partial expression may occur, more commonly in black persons. Individuals with e variant phenotypes can make antibodies to epitopes they lack. While some of these antibodies may be specific for an antigen, e.g., hrB, others, like anti-Rh17 (anti-Hro), show broader specificity, compatible only with D– – and Rhnull red blood cells (RBCs). Anti-Rh17 in persons of the D– – phenotype has been reported to

Marla C. Brumit, Gary E. Carnahan, James R. Stubbs, Jill R. Storry, Marion E. Reid

Immunohematology, Volume 18 , ISSUE 2, 40–42

Report | 01-December-2019

Should blood donors be routinely screened for irregular antibodies?

was used for calculating the probability of antigen-antibody reaction from donors in this blood bank. Total alloantibodies (438) and CSAA frequency (138) were significantly higher in women than men (p < 0.01). Seventy-four percent of CSAA found in women came from the Rh blood group system. Calculated probability of generating antigen-antibody reaction using plasma only from male donors was estimated as 20.55 episodes for every 100,000 donations, and the probable number of events per year was

Michel Andrés García, Leonardo Bautista, Fernando Palomino

Immunohematology, Volume 28 , ISSUE 2, 60–66

Case report | 14-October-2020

Red blood cell antigen changes in malignancy: case report and review

Jeffrey L. Winters, Dianna S. Howard

Immunohematology, Volume 17 , ISSUE 1, 1–9

Article | 17-February-2021

Concordance of two polymerase chain reaction–based blood group genotyping platforms for patients with sickle cell disease

assays for antigens in the LW and Scianna blood group systems, whereas ID CORE XT does not. Similarly, ID CORE XT includes an assay for antigens in the Cartwright blood group system, whereas HEA BeadChip does not. More importantly, the assays interrogate some different single nucleotide variants (SNVs), especially in the Rh blood group system. The predicted phenotype result takes into account the presence or absence of variant sequences at polymorphic positions as well as population frequency of

C.A. Sheppard, N.L. Bolen, G. Meny, M. Kalvelage, G. Ochoa-Garay

Immunohematology, Volume 36 , ISSUE 4, 123–128

Article | 22-January-2021

Routine indirect antiglobulin testing of blood donors—a further step toward blood safety: an experience from a tertiary care center in northern India

antibodies among these blood donors was found to be 0.18 percent (19 of 10,390). Of the 19 alloimmunized donors, 16 (84.2%) were male (alloimmunization rate 0.16%, 16 of 9959) and 3 (15.8%) were female (alloimmunization rate 0.69%, 3 of 431) (p = 0.01; chi-square test). In our study, the most frequent alloantibodies identified were of the Lewis blood group system (17 of 25 [68%] in 14 of the 19 alloimmunized donors). The second most common alloantibodies belonged to the Rh blood group system (4 of 25 [16

S. Malhotra, G. Negi, D. Kaur, S.K. Meinia, A.K. Tiwari, S. Mitra

Immunohematology, Volume 36 , ISSUE 3, 93–98

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